Progress 10/01/14 to 09/30/15
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Germplasm release of Fla. 8923, Fla. 8624 and Fla. 8638B will provide breeders with additional resources for development of TYLCV-resistant cultivars.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Hutton, S.F., Y. Ji, and J.W. Scott. 2015. Fla. 8923; a tomato breeding line with begomovirus resistance gene Ty-3 in a 70kb Solanum chilense introgression. HortScience 50:1257-1259.
Scott, J.W., S.F. Hutton, and J.H. Freeman. 2015. Fla. 8638B and Fla. 8624 tomato breeding lines with begomovirus resistance genes ty-5 plus Ty-6 and Ty-6, respectively. HortScience 50:1405-1407.
Caro, M., M.G. Verlaan, O.Julian, R. Finkers, A.-M. A Wolters, S.F. Hutton, J.W. Scott, R. Kormelink, R.G.F. Visser, M.J. Diez, A. Perez-de-Castro, Y. Bai. 2015. Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm. Mol. Breed. 35:132.
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?5 presentations (2 postdoc, 2 grad students, PI) at the 2014 Tomato Breeders Roundtable, Mills River, NC. 2 presentations (1 grad student, PI) at the 2014 annual meeting of the American Society of Horticultural Sciences What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Improved molecular markers with tight linkage to the I-3 gene are allowing more efficient selection for fusarium wilt race 3 resistance. Likewise, better markers linked to the Ty-2 gene have been developed and are being utilized. A minimal Ty-3 introgression continues to be advanced into elite germplasm.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Yang, X., M.Caro, S.F. Hutton, J.W. Scott, Y. Guo, X. Wang, H. Rashid, D. Szinay, H. de Jong, R.G.F. Visser, Y. Bai, and Y. Du. 2014. Fine Mapping of the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-2 on Chromosome 11 of Tomato. Molecular Breeding. DOI 10.1007/s11032-014-0072-9
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Hutton, S.F., J.W. Scott, and G.E. Vallad. 2014. Association of the fusarium wilt race 3 resistance gene, I-3, on chromosome 7 with increased susceptibility to bacterial spot race T4 in tomato. J. Amer. Soc. Hort. Sci. 139:282-289.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Li, J. and S.F. Hutton. 2014. Indel markers associated with the I-3 introgression for resistance to fusarium wilt race 3. Rept. Tomato Genet. Coop. 64:19-24.
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Progress 10/01/12 to 09/30/13
Outputs
Target Audience: Tomato growers and packers in Florida. Tomato Breeders Changes/Problems: One of the objectives was to develop freeze tolerant tomatoes. This was based on some early 1980 work with a line of tomato that appeared to have freeze tolerance. Experimentation in 2011-2012 did not indicate that this line did indeed have freeze tolerance so this objective has been dropped. What opportunities for training and professional development has the project provided? Aline Frasca is doing a MS thesis on CGH tomato evaluation. Matt Mattia is working onthe effects of immature fruit color on soluble solids and yellow top. How have the results been disseminated to communities of interest? Sam Hutton gave a talk on CGH and other tomato breeding projects to the Florida tomato industry in Naples Florida inSeptember at the Florida Tomato Institute. He also presented research on our TYLCV resistance work at; the Tomato Breeders Roundtable meeting in Thailand in February, at the International Tomato Disease Symposium in Orlando, Florida in June, and at the American Society of Horticultural Science in Palm Desert California in July. J Scott presented talks on bacterial spot resistance breeding at the Tomato Breeders Roundtable in Thailand in February and at the International Tomato Disease Symposium in Orlando, Florida in June. Scott also gave a talk on CGH tomatoes and bacteraial spot resistant tomatoes at the Florda Seed Association meeting at Inverness Florida in June. He presented a tomato breeding talk on breeding tomaotfor disease resistanceat a soil borne disease symposiumin Stellenbough, South Africa in September and anotheron fruit qualityat an International Plant Breeding Congress in Antalya, Turkey in November. What do you plan to do during the next reporting period to accomplish the goals? Major breeding line trials will be conducted on 3 farms in Dade County and in the spring and fall at GCREC. The best inbreds and hybrids will be tested in replicated yield trials in the spring and fall crop. Crosses will be made between elite parents for the various projects to assess new hybrids and/or recombine traits for inbred development. Emphasis will be placed on development of TYLCV resistant lines with good horticultural characteristics, bacterial spot race T4 and T3 tolerance, and improved fruit quality. MAS of several disease resistance genes into elite parents will continue. Our new source of Ty-3 TYLCV resistance with a small introgression is being emphasized as a donor and some of these lines will be released to the seed industry this year. We will test lines with various combinations of the resistance genes Ty-3, Ty-4, and ty-5 for resistance to tomato mottle virus (ToMoV). We will continue to fine map the Ty-4 gene. Tomato spotted wilt experiments will be repeated at NFREC with Josh Freeman as disease incidence was low again in 2013. We are doing some fine mapping of Sw-7 and elucidating the inheritance along with determining if there is no fruit blotch of Sw-7 lines as there is with Sw-5. In Dade County we are repeating a large experiment to determine the inheritance of graywall and find molecular markers linked to resistance. We will finish incorporation of late blight genes Ph-2 and Ph-3 into elite inbreds. Late blight resistant hybrids will be evaluated at GCREC and off-site. A major and even greater emphasis will be on developing compact growth habit (CGH) tomato inbreds with jointless pedicels. Some CGH lines such as Fla. 8924 appear to be getting closer to having commercial parental potential and can be used as open-pollinated lines for experiments. Hybrids of these will be tested vigorously and the best lines will be used as recurrent parents to begin incorporating resistance to TYLCV, bacterial spot and fusarium crown rot. This may be a year off. Crosses will eventually be done with these CGH lines to incorporate crimson and improved flavor. The bacterial wilt resistant program continues to be of interest as some lines also have tolerance to bacterial spot and the fruit size is very large in most the lines. Line Fla. 8872 came out of this project and although it does not have bacterial wilt resistance, it has looked good as a hybrid parent in combinations tested in 2012 and 2013.Recent emphasis has been placed on developing improved heat-tolerant inbreds and some hybrid crossing was done last fall with some interesting new inbreds, especially Fla. 8925. Hybrids with these will be tested further in 2014. There is still not an outstanding heat tolerant hybrid released and such a hybrid could greatly benefit the Florida tomato industry. We also have great interest in the genetically modified hybrids carrying the Bs2 pepper gene. Some hybrids that have Bs2 and resistance to TYLCV, fusarium crown rot, or fusarium wilt race 3 looked outstanding in fall 2012 and 2013. The bacterial spot resistance and yield of many Bs2 hybrids is outstanding. Our freeze tolerance experiments did not show that we had a good source of resistance and this objective has been dropped. I have the MS student (Matt Mattia) who worked on that working on another project dealing with immature fruit color and possible associations with fruit sugar production. Recently, we have found some interesting results in that project. Two major experiments involve trying to find molecular markers linked to graywall resistance and to bacterial wilt resistance. For the former hundreds of RILs are being grown on two farms in Dade county and if graywall expression is good we should be able to locate the resistance genes based on target regions we presently have in comparing polymorphisms between resistant and susceptible varieties. This is part of an objective of the Specialty Crops Grant. For bacterial wilt we have target regions for two populations via SNPs from the SolCAP project. Over 600 RILs will be screened for resistance by Mathews Paret of NFREC. We are looking into field tests in Taiwan and India through a colleague at United Genetics Seed Co. If these trials are successful we should be able to identify the QTL that are important to having resistance.
Impacts
What was accomplished under these goals?
Fla. 8872B, an inbred with outstanding fruit size and a strong vine performed very well in inbred trials as it did last year. It was also a parent in several hybrids which did well in spring 2013 as was the case last year. A hybrid with Fla. 8870, a bacterial wilt tolerant inbred, looked good in spring 2013 and further testing of Fla. 8870 and hybrids derived from it will continue. Fla. 8923 has outstanding fruit size and yielded very well in spring 2013. It has tolerance to TYLCV provided by the Ty-3 gene that was backcrossed from our short introgression source. Fla. 8923 may be released as a breeding line next year. Fla. 8925 is a new heat-tolerant (HT) parent line that may have potential in producing HT hybrids for the Florida fall market. It also hasvery good flavor.Fla. 8916 is a jointless compact growth habit (CGH) inbred that yielded over 1400 boxes per acre in a single pick in a grower trial. However, the firmness was not acceptable. Also in spring 2013 it and many other inbreds had off shapes and rough blossom scars due to cold weather. Our best advanced inbred under these severe conditions was Fla. 8924. Fla. 8924 also looked good in fall 2013. Several early generation CGH selections were made that had good shape and smoothness in the spring. Hybrids are being made between the best CGH parents in this program that is being emphasized more than ever.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Verlaan, Maarten G., Samuel F. Hutton, Ragy M. Ibrahem, Richard Kormelink,
Richard G. F. Visser, John W. Scott, Jeremy D. Edwards, and Yuling Bai. 2013.
The Tomato Yellow Leaf Curl Virus Resistance Genes Ty-1 and Ty-3 Are Allelic and Code for
DFDGD-Class RNADependent RNA Polymerases. PloS Genetics http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003399
Ozores-Hampton, M., A. Coelho Frasca, J. Scott, and S. Hutton. 2013. Compact growth habit tomatoes. EDIS HS1231. Sept. 2013.
http://edis.ifas.ufl.edu/hs1231
Scott, J.W. 2013. Fla. 8111B; a large-fruited, globe shaped tomato breeding line. Rpt. Tomato Genet. Coop. 63:31.
J.W. Scott and S.F. Hutton. 2013. Fla. 8233; a germplasm line with partial resistance to
bacterial spot races T1, T2, T3, T4 and Xanthomonas gardneri. Rpt. Tomato Genet. Coop. 63:32.
Scott, J.W. 2013. Breeding for soil-borne disease resistance in tomato; the bitter and the sweet.
Proc. Soilborne plant diseases symposium, Stellenbosch, South Africa 44-53.
Hutton, S.F., J.W. Scott, and B.M. Santos. 2013. Developing machine harvestable tomatoes; and other highlights from the UF breeding program. Proc. Florida Tomato Institute. 8-9.
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Progress 10/01/11 to 09/30/12
Outputs
OUTPUTS: Testing of Florida adapted tomato hybrids containing the Bs2 gene from pepper was done for two seasons and the yields of Bs2 containing hybrid Fla. 8314 was twice that of Fla. 8314 without Bs2 and Florida 47 a widely grown variety. Advances were made in developing tomatoes that do not require staking and tying due to their lack of apical dominance due to the brychytic gene. These breeding lines also have the jointless pedicel gene that can allow for mechanical harvest and thus overall significant monetary savings for the Florida tomato growers. Hybrids of the best lines are presently being made. An emerging inbred Fla. 8872 was tested in six hybrids in the spring and all six were in the top ten yielding varieties in the trial. Further testing is now underway. A new TYLCV resistance gene was located on chromosome 10 and will now allow for marker assisted selection to be used in backcrossing resistance into elite inbred lines. Several potential QTL for bacterial spot resistance were identified in populations derived from PI114490. Further testing will verify which QTL to use to develop improved tolerant breeding lines. PARTICIPANTS: The following have worked on this project: Dr. Supaporn Ieamkhang, a plant pathologist from a university in Thailand did a study leave for 6 months; Postdoctoral scientists Sarah Smith and Reza Shekate-band; Agricultural technicians Jose Diaz, Rudy Jones, Heather Hammons,Ashley Martin, Kerri Druffel,Dolly Cummings, and Tim Davis; and graduate students Matthew Mattia and Jian Lee.We have partnered with the Two Blades Foundation on the pepper Bs2 gene work. University of Florida collaborators are: plant pathologists Gary Vallad, Jerry Bartz and Jeffrey Jones and Horticulturalist Steve Olson. Dr. Elisabeth Balswin of the USDA has helped with our flavor work. TARGET AUDIENCES: Florida tomato growers, tomato growers outside of Florida in the US and around the world, tomato breeders, Agricultural Extension agents, and tomato consumers are primary audiences. Efforts include release of improved tomato germplasm, published papers of research results, and presentation of research findings at academic and grower oriented meetings. PROJECT MODIFICATIONS: Our approach has not altered drastically. With the availability of genomic information we are doing even more molecular work than previously. This is because we now have the ability to find genes of interest due to more saturated marker coverage that we had previously. We can also do more efficient fine mapping to precisely locate genes and remove unneeded chromosome segments that may cause linkage drag.
Impacts
The previously released tomato hybrid TASTI-LEE is expanding in supermarkets across the Eastern US and began in California. Acreage is increasing and the volume of tomatoes being sold is increasing. This tomato has good flavor and color due to increased lycopene that has numerous health benefits as an antioxidant that helps prevent several cancers and heart disease. These traits are important to consumers. Several tomato growers in Florida are making profits growing the variety because prices are consistently high unlike general tomato varieties that are grown.
Publications
- Sung-Chur Sim, Allen Van Deynze, Kevin Stoffel, David S. Douches, Daniel Zarka, Martin W. Ganal, Roger T. Chetelat, Samuel F. Hutton, John W. Scott, Randolph G. Gardner, Dilip R. Panthee, Martha Mutschler, Jim Myers, David M. Francis. 2012. High-density SNP genotyping of tomato (Solanum lycopersicum L.) reveals patterns of genetic variation due to breeding. PLoS ONE. 7:e45520.
- Horvath DM, Stall RE, Jones JB, Pauly MH, Vallad GE, Dahlbeck, D, Staskawicz, BJ, Scott, JW. 2012. Transgenic Resistance Confers Effective Field Level Control of Bacterial Spot Disease in Tomato. PLoS ONE 7(8): e42036. doi:10.1371/journal.pone.0042036
- Hutton, Samuel F., Jay W. Scott, and David J. Schuster. 2012. Recessive resistance to Tomato yellow leaf curl virus from the tomato cultivar Tyking is located in the same region as Ty-5 on chromosome 4. HortScience 47(3):324-327.
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