Recipient Organization
IOWA STATE UNIVERSITY
2229 Lincoln Way
AMES,IA 50011
Performing Department
Veterinary Medicine
Non Technical Summary
Oral fluids have become a good sample to test for pathogens within a population of pigs and are increasingly used for surveillance and diagnostic purposes. One of the main advantages of using oral fluids is that a higher percentage of pigs can be sampled at a reduced cost. If successful, the proposed project will allow producers to test oral fluid samples for antibodies to a variety of important swine pathogens with the ability to expand and custom fit this menu to specific needs of individual producers and production systems at a reduced cost in comparison to testing for the same pathogens on multiple enzyme-linked immunosorbent assays (ELISAs). Moreover, the Luminex MIA protocol is thought of as being slightly more sensitive compared to regular ELISA assays which will likely be an additional advantage of the Luminex technology on oral fluid samples which contain lower levels of antibodies.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Goals / Objectives
The objective of this project is to evaluate the Luminex MIA for simultaneous detection of antibodies against common swine pathogens in oral fluid samples.
Project Methods
Samples known to be positive for certain swine pathogens will be used from previous swine studies done in our research lab. In this specific preliminary study, we will partner with a commercial company that specializes in large scale production of coated beads for commercial use and also on improvement of assay performance. The experience of this company with human applications of the Luminex platform will help with pursuing our overall goal, to have a validated, fast, reliable, and inexpensive assay available for detection of antibodies against important swine pathogens in a single oral fluid sample with the ability to expand the set of pathogens detected in the near future. Our goal is to have preliminary test results available to apply for subsequent funding. Descriptive statistics will be generated using JMP 7.0.2 to check for normal distribution of the data. Where possible, e.g., influenza virus, the evaluation will include a full factorial analysis at the level of isolate, subtype, treatment (vaccinate or non-vaccinate), and day post inoculation.