Performing Department
(N/A)
Non Technical Summary
Within the last decade, obesity has increased dramatically on a global scale. Poor diet habit is one of the most common causes of excessive weight gain in today?s society and dietary intervention has been proven to be an effective approach to reduce the risk of obesity and related type 2 diabetes. We and others have found that rosemary extract has the antiobesity and antidiabetic effects. The objectives of this project are to identify the active components in rosemary extract and the effective dose of the active components and rosemary extract to reduce weight gain and type 2 diabetes in high-fat-fed mice, and to further understand the underlying molecular mechanisms. The development of this proposal is to build North Carolina A&T State University?s research capacity in the area of bioactive food components for prevention of obesity and related type 2 diabetes. New research activities created in this project will strengthen North Carolina A&T State University?s research capacity to study the beneficial health effects of functional foods and their bioactive components and to work with food industries to develop value-added functional foods to prevent chronic diseases, such as obesity and related type 2 diabetes.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Goals / Objectives
The goals of this project are to identify the active components in rosemary extract and the effective dose of the active components and rosemary extract to reduce weight gain and type 2 diabetes in high-fat-fed mice, and to further understand the underlying molecular mechanisms. Our specific objectives are as follows: Objective 1. To determine the dose-dependent inhibitory effects of rosemary extract and its major components on obesity and type 2 diabetes in high-fat-fed mice by assessing a) Body weight and body fat gain b) Fasting blood glucose levels and plasma insulin levels c) Liver triglyceride levels Objective 2. Elucidate the mechanisms of inhibition of body weight gain and type 2 diabetes by rosemary extract and its active components in high-fat-fed mice. Using samples from Aim 1, we will examine the effects of the different treatments on a) Fecal lipids; b) Plasma inflammatory markers; c) Plasma leptin and adiponectin levels; d) Adipose tissue adiponectin and peroxisome-proliferator-activated-receptor (PPAR)-gamma levels. The expected ourputs from this project will be to observe inhibitory effects of carnosic acid enriched rosemary extract and its three major components, carnosic acid, carnosol and methoxy carnosic acid, on body weight and body fat gain and type 2 diabetes in high-fat-fed mice. We also expect to present our results at scientific meetings and publish them in peer-reviewed journals.
Project Methods
We will purify the three major components in rosemary extract for the proposed animal study in objective 1 and confirm their structures using MS and NMR. Then we will conduct the proposed animal study. We will recording the diet and body weight of the mice every week, and collecting fecal samples at 0, 5, 10, 12, 14, and 16 weeks of treatment. At 0, 5, 10, 12, 14, and 16 weeks of treatment, we will measure fasting blood glucose levels. After 16 weeks of treatment, we will sacrifice the mice and collect plasma and tissue samples for measurement of biomarkers discussed in Objective 2. For objective 2, we will identify the metabolites of the major components in rosemary extract and develop an LC/MS method to quantify their plasma and tissue levels. By accomplishing this project, we will present our results at scientific meetings and publish them in peer-reviewed journals. The accomplishment of stated objectives, results and measurable outcomes will be evaluated by the formed review committee focusing on the following questions: a) Does this project indeed build the research capacity of NCA&TSU b) Are students at NCA&TSU trained in this project as planned c) Is the proposed research conducted as planned