Recipient Organization
UNIV OF PENNSYLVANIA
(N/A)
PHILADELPHIA,PA 19104
Performing Department
School Of Veterinary Medicine
Non Technical Summary
Colitis is a common disease of the horse, and often results in endotoxemia that leads to intravascular fluid derangement, lactic acidosis, hemodynamic collapse, and death. Despite the prevalence of this disease, there are still no commonly used stall-side assays to identify endotoxemia, and therapy of horses is often complicated by the lack of goal-directed therapy for hypovolemia and hypoperfusion.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Goals / Objectives
To validate two stall-side assays for plasma endotoxin: the neutrophil chemiluminescence assay, and viscolelastic coagulation testing as a surrogate marker for endotoxin. To compare exogenous 13C-labelled lactate clearance in healthy horses and those with endotoxemia. To validate non-invasive echocardiographic measures of stroke volume and cardiac output in horses with endotoxemia.
Project Methods
All 3 objectives will be met using data from a crossover design study using 6 healthy adult horses. After acquisition of institutional approval, the horses will either undergo endotoxemia induction using purified E. coli LPS at 50ng/kg infused intravenously over 30 minutes, or receive a placebo infusion of normal saline. There will be a minimum 2 week wash-out period, and each horse will then undergo the same procedures in a cross-over fashion. Investigators will be blinded to group assignment until data analysis.