Source: UNIVERSITY OF WYOMING submitted to
TRICHOMONIASIS IN WYOMING BEEF CATTLE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0224559
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jan 1, 2011
Project End Date
Sep 30, 2013
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF WYOMING
1000 E UNIVERSITY AVE DEPARTMENT 3434
LARAMIE,WY 82071-2000
Performing Department
Veterinary Sciences
Non Technical Summary
Bovine trichomoniasis is a worldwide disease caused by the parasitic protozoan Tritrichomonas foetus. This sexually-transmitted disease leads to infertility, abortion, and womb infections in female cattle. It is been found in many US states, especially in the Midwest and West, including Wyoming. It results in large economic losses for the cattle industry, particularly in cow-calf operations that depend on natural service. The Wyoming State Veterinary Laboratory (WSVL) has been providing trichomoniasis testing for over three decades. The state has regulated the disease for the last 10 years through the Wyoming Livestock Board (WLSB)-mandated testing of bulls. A total of 14 of 23 Wyoming counties in the state had positive herds in the three years between 2007 and 2009. In spite of the state-testing program, the disease persists and important questions remain unanswered. What is the true prevalence of the disease in Wyoming What risk factors affect persistence of the disease, in spite of mandatory testing, as well its prevalence and spread to new ranches What additional effective measures need to be taken to control the disease in our state Our long-term goal is to eradicate the disease in Wyoming. The purpose of this proposal is to comprehensively investigate trichomoniasis status in Wyoming beef cattle and develop an integrated, yet affordable and acceptable, approach to control the disease. Two specific aims are included in the proposal. AIM 1: To identify risk factors associated with beef cattle trichomoniasis. This is achieved by a survey of Wyoming beef cattle ranchers using a questionnaire. AIM 2: To perform a longitudinal investigation of trichomoniasis on production facilities of various sizes. Sampling of beef cattle ranches of various operations from small through medium to large scales will be carried out. Bulls will be tested annually prior to and post breeding season in all ranches, and cows will be monitored for pregnancy status and infections on the ranches where infected bulls have been identified. The significance of the project is to provide an epidemiological snapshot of trichomoniasis in Wyoming and to provide WLSB with better information so the state rules governing the disease will be refined so that it can be eradicated in Wyoming.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3133310111030%
3133310117020%
3134050111030%
3134050117020%
Goals / Objectives
Objectives of this current grant application are to better define the epidemiology of trichomoniasis in beef cattle in Wyoming and refine the state rules governing the disease so that it is both affordable and integrated with production practices in the state. Two specific aims are included. AIM 1: To identify risk factors associated with the spread and persistence of beef cattle trichomoniasis. This will be achieved by a survey of Wyoming beef cattle ranchers using a questionnaire. AIM 2: To undertake a longitudinal investigation of trichomoniasis using a two-year testing program on representative properties of varying sizes. Bulls will be tested yearly prior to and post breeding season in all ranches, and cows will be monitored for pregnancy status and infections on ranches with infected herds.
Project Methods
AIM 1: To identify risk factors associated with the spread of trichomoniasis on Wyoming ranches. This will be approached through a questionnaire survey of ranchers. It will address trichomoniasis status in the last three years, bull ages and testing, vaccination of cows against venereal diseases and fence repair. The operations with 1-49 head, 50-99 head, 100-499 head and >500 head cows statistically required for the survey will be 104, 62, 122 and 26, respectively. These represent 4.5%, 9.3%, 8.1% and 7.9% of all operations for 2009, the latest data available. Once questionnaires are collected, they will be assessed for completeness. A telephone follow-up will be done for each incomplete questionnaire. Only complete ones will be used for analyses. Answers to each question will be statistically analyzed, and the correlation with trichomoniasis calculated. We anticipate some factors, i.e., risk factors, will be correlated with this disease in beef cattle. This will indicate risk factors contributing to the prevalence and spread of trichomoniasis. AIM 2: Longitudinal investigation for trichomoniasis on selected properties of varying sizes. Representative ranches of small, medium and large operations will be picked at random across the state in a field study. All bulls included in the study will be tested. In contrast, cows will be randomly sampled and tested in trichomoniasis-positive ranches. Positive females will be separated from the remainder of herds, and their status will be monitored in the next two years by collecting samples in the alternate months.

Progress 01/01/11 to 09/30/13

Outputs
Target Audience: Beef producers and researchers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The main objective of this study was to identify herd-level risk factors for trichomoniasis in Wyoming beef cattle. A questionnaire was sent to all Wyoming beef cattle producers. The overall response proportion was 23.4%. Questionnaires were returned from producers throughout the state in different geographic regions and with various herd sizes. 863 questionnaires were analyzed for correlation between the disease endemism and 25 variables. Tritrichomonas foetus infections were found to be significantly (P<0.05) associated with neighboring a positive herd(s), grazing on public allotments and commingling with other herds. In addition a delay in fixing broken fences approached statistical significance (P=0.078). This study provides producers with valuable information and useful suggestions on how to effectively control and reduce the risks of bovine trichomoniasis.

Publications

  • Type: Theses/Dissertations Status: Published Year Published: 2013 Citation: Risk factors associated with bovine trichomoniasis in beef cattle determined by a questionnaire survey. Jin, Yinzhu. University of Wyoming, ProQuest, UMI Dissertations Publishing, 2013. 1539837.


Progress 01/01/12 to 12/31/12

Outputs
OUTPUTS: 1. A questionnaire along with a consent form was mailed to all cattle ranchers in Wyoming, which totaled 5,498. A follow-up postcard was sent two weeks later. 1,288 responses were collected, which is 23.4%. 2. Analysis of the data of the questionnaire survey is done. 3. Invitations and contracts were sent to all seven ranchers with positive herds in the last three years identified by the questionnaire survey. This was intended for the breeding season 2011-2012 of the 2nd phase of longitudinal follow-up of the positive herds by testing all bulls and some open cows. Three decided not to participate. 4. Three ranchers tested all their bulls three times. The numbers of bulls tested are 48, 22 and 10 bulls, respectively. One rancher had his 23 bulls tested once. All bulls were negative. 5. Invitations and contracts were sent to the four ranchers again this past October by certified mail with return receipt. This was for the breeding season of 2012-2013. PARTICIPANTS: Yao, Chaoqun (PI). Jin, Yinzhu-graduate student,data analysis. TARGET AUDIENCES: Cattle ranchers, over 5,000 alone in Wyoming. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Bovine trichomoniasis has been endemic in many western US states for decades. The main objective of this study was to identify risk factors associated with positive herds with Tritrichomonas foetus in the Wyoming beef cattle operations. This cross-sectional study was conducted by a questionnaire-based survey, targeted at all Wyoming beef cattle operations. In all, 863 questionnaires from ranchers in various geographic regions and having various herd sizes were included to analyze the relationship between the disease and 25 variables. Tritrichomonas foetus infection was associated with several factors including using allotments fenced off with positive herds, public allotments, commingling with other herds and a delay in fixing broken fences for more than a week. This study provides ranchers with useful information and suggestions on how to better control and reduce the risk of the bovine trichomoniasis, a disease that is influencing profitability of the beef cattle industry.

Publications

  • Yao, C. 2013. Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle Journal of Medical Microbiology, 62:1-9
  • Yao, C. 2012. Opportunistic human infections caused by Tritrichomonas species: A mini-review. Clinical Microbiology Newsletter, 34:127-131
  • Yao, C., Bardsley KD, Litzman EA, Hall ML, and Davidson MR. 2011. Tritrichomonas foetus infection in beef bull populations in Wyoming. Journal of Bacteriology & Parasitology, 2:117. doi:10.4172/2155-9597.1000117