Source: PENNSYLVANIA STATE UNIVERSITY submitted to NRP
JDIP: JOHNE`S DISEASE INTEGRATED PROGRAM IN RESEARCH, EDUCATION AND EXTENSION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
AFRI COMPETITIVE GRANT
Reporting Frequency
Annual
Accession No.
0224408
Grant No.
2011-85204-30025
Cumulative Award Amt.
$1,200,000.00
Proposal No.
2010-04784
Multistate No.
(N/A)
Project Start Date
Feb 15, 2011
Project End Date
Feb 14, 2015
Grant Year
2011
Program Code
[A5111]- Global Food Security: Minimizing Losses from Dairy Diseases with Major Impact on Production, Marketing, and/or Trade
Recipient Organization
PENNSYLVANIA STATE UNIVERSITY
208 MUELLER LABORATORY
UNIVERSITY PARK,PA 16802
Performing Department
Veterinary & Biomedical Sciences
Non Technical Summary
We propose to continue an integrated program in Johne's disease (JD) research focused on developing a strong translational pipeline of new diagnostic tests, vaccine candidates, and strategies to manage, prevent and control the disease. Four major research themes are organized into projects led by internationally renowned scientists. Project 1 focuses on understanding JD epidemiology and the process of disease transmission; Project 2 seeks to develop and implement new generations of diagnostic tests and methods for M. paratuberculosis (MAP) strain differentiation; Project 3 focuses on improving our understanding MAP basic biology and elucidating pathogenesis mechanisms; Project 4 identifies basic strategies to enhancing the T and B cell immune response to MAP and initiates programs to develop new vaccines. These projects are supported by Scientific Cores in Biostatistics and Epidemiology; Diagnostics and Strain Differentiation; Gene and Protein Expression; and Animal Experimentation. JDIP promotes linkages with public and private stakeholders to foster transitional research and promote maximal use of core facilties, as facilitated by an Administrative Core and a Communications Extension Core. An External Advisory Board advises JDIP on programmatic maters and ensures JDIP stays true to its mission and capitalizes on recent scientific advances. This group reviews scientific programs and provides advice to the Steering Committee. Overall, JDIP serves to bring together scientists and stakeholders with a shared vision and well defined plan to support and facilitate research, extension and education activities to enhance animal bio-security by addressing well-documented and emerging needs in JD.
Animal Health Component
40%
Research Effort Categories
Basic
40%
Applied
40%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3113310110010%
3113410109010%
3113410110020%
3113410117010%
3113410302020%
3114010110010%
3114010117010%
3114010302010%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3310 - Beef cattle, live animal; 4010 - Bacteria; 3410 - Dairy cattle, live animal;

Field Of Science
1090 - Immunology; 3020 - Education; 1100 - Bacteriology; 1170 - Epidemiology;
Goals / Objectives
Members of the JDIP consortium subscribe to three basic assumptions regarding the program: First, we believe that the success of JDIP depends on the creation and maintenance of functional and flexible research teams that are interdisciplinary and multi-institutional, and use cutting-edge technology. Second, we believe that JDIP must help reduce timelines for translating basic science research in JD into useful products and procedures by bringing together teams of interdisciplinary scientists who are supported by enabling core facilities. Third, we believe that JDIP must strive to provide real-world solutions prevent or mitigate the losses associated with JD based on our cumulative scientific excellence. With this background, six Strategic Objectives for JDIP were developed and endure: (i) Organize JDIP as a dynamic research and development consortium with the aim of supporting and facilitating investigator-directed research on JD; (ii) Create and maintain comprehensive scientific core facilities to support JD research and training activities; (iii) Help develop translational research capacity for developing and validating diagnostic tests, vaccines, and disease management concepts for JD; (iv) Serve as a resource for qualified investigators from academia, regulatory agencies, and industry so as to enable basic and applied research for testing and evaluating diagnostics, vaccines, and disease management concepts for JD; (v) Help create a mechanism to provide developmental support to JD investigators; and, (vi) Provide information and scientific support for the development of a strong education and extension program for JD prevention and control.
Project Methods
During the current cycle of funding of JDIP, we propose to work towards achieving our programmatic objectives by continuing to identify and prioritize key needs and opportunities and charging the leading experts in the field with the responsibility of developing collaborative work plans to realize these goals. We will continue the series of research projects and core facilities that were vetted through a rigorous process of prioritization and a scientific peer-review process by the leaders in the field and external stakeholder review. Importantly, each project and core facility is evaluated on an annual basis through the same rigorous process of scientific and external stakeholder review. Based on documentation of progress towards achieving goals and programmatic needs, funding support is provided per original plan, modified, or redirected to a higher priority program or project. Overall, we believe that JDIP and the CAP program has empowered the JD community, enabled programmatic integration on an unprecedented scale, and provided flexibility in making funding decisions through regular peer-review and stakeholder input. Together, these not only differentiates us from the other USDA-NIFA competitive programs, but also enables an extraordinary leveraging of funds and return on investment for the USDA not typically possible through traditional funding mechanisms. We propose to continue our five research projects. Project 1 will focus on understanding JD epidemiology and process of disease transmission; Project 2 will seek to develop and implement new generations of diagnostic tests for JD; Project 3 will focus on improving our understanding of Map biology and mechanisms of pathogenesis; Project 4 will investigate host genetic mechanisms of disease susceptibility, elucidate the early immune response to Map, and initiate programs to evaluate and develop new generations of vaccines; and Project 5 will focus on developing and delivering education and extension material for JD. We note that the work plans proposed for the project are dependent upon continued relevance to programmatic needs, documented progress to achieving project goals and milestones, and subject to competitive peer-review. We propose to continue our four state-of-the-art scientific core facilities. Core 1, the Epidemiology and Biostatistics Consulting Core, providing the community with consulting services and training programs for JD related biostatistics and epidemiological study design; Core 2, the Diagnostics and Strain Differentiation Core, providing services, reagents, and standardized protocols for diagnosis and strain differentiation of Map; Core 3, the Genomics, Antibodies, and Proteomics Core, providing state-of-the-art genomics and proteomics reagents and resources, as well as services and facilities for the production of antibodies and other immunological reagents for Map and JD research; Core 4, the Animal Models and Facilities Core, developing and providing access to standardized animal models as well as access to state-of-the-art animal facilities for JD research; and Core 5, the Administrative Core, responsible for the oversight of the JDIP program.

Progress 02/15/11 to 02/14/15

Outputs
Target Audience: JDIP targets the following audiences: scientists, veterinarians, producers, regulatory agencies, educators, policy makers, and the general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Drs. Ken Olson and Jeannette McDonald have worked on extension aspects of JDIP, and have provided education, certification and recertification for veterinarians in collaboration with the Voluntary Bovine Johne's Disease Control Program (VBJDCP), providing tools for producers, as well as effective communication between scientists, producers, industry partners, and USDA. Moreover, in collaboration with DHIA, three on-line modules covering milk sampling and ELISA testing for MAP were developed and are currently used by field and lab technicians. Tools for producer programs include an educational module on JD for dairy (English and Spanish), beef and small ruminant producers, and have been expanded to work in Italy. How have the results been disseminated to communities of interest? Results have been distributed through peer-reviewed publications, presentations at national and international conferences, extension meetings, communications in the lay press and radio shows, and through our web-site, www.mycobacterialdiseases.org. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Throughout the project, JDIP continued to meet and surpass its project objectives. Our research endeavors have focused on strategies to manage, prevent and control the disease, and have continued improvement of our understanding of Mycobacterium avium subsp. paratuberculosis (MAP). Some of the JDIP's accomplishments are as follows: (1) Development of the epidemiologic mathematical models to help understand the dynamics of MAP transmission on-farm in order to identify critical control points in the transmission chain; (2) Development and validation of alternative sampling and testing strategies for detection of infected animals and herds that are being adopted by the national voluntary control program for JD; (3) Development and evaluation of standard laboratory protocols for MAP culture, and PCR for reducing timelines for rapid detection of infected animals; (4) Advancement in better understanding of the molecular mechanisms of infection; (5) Establishment and evaluation of standard approaches for the validation of vaccine candidates and vaccine efficacy; (6) Detection of key genes, proteins and lipids in MAP for development of the next generation of diagnostic tests and vaccines; (7) Establishment of an on-line JD veterinary certification program; (8) Development of community resources including MAP isolates, serum samples and other clinical material for the development and validation of diagnostic tests, genomic microarrays, recombinant proteins, and mutant strain banks of MAP in order to identify potential vaccine candidates; (9) Application of an established three-step framework of a Bayesian mathematical model of infection in data; (10) Establishment of benchmarks for diagnostic assays and usage of STandards for Reporting of Animal Diagnostic Accuracy Studies - paratuberculosis (STRADAS) guidelines for transparent and complete reporting, and continued research on the newly identified MAP-specific monoclonal antibody 17A2, which for the first time can differentiate MAP from other Mycobacterium species and subspecies; (11) Development of a better understanding of the molecular mechanisms of infection with emphasis on the role of super-shedders; (12) Development of the extension, outreach, and distribution of education and outreach materials to stakeholders; (13) Establishment of a sample repository from JDIP samples for further testing and evaluation; (14) Establishment of a multi-institute vaccine efficacy trial; (15) Finally, expansion of the JDIP model to include bovine tuberculosis; JDIP investigators helped develop a new Mycobacterial Diseases in Animals multistate initiative (MDA MI). MDA functions to provide information and tools to producers and make science-based recommendations to policy makers. MDA hosts its annual meeting of the Multistate Research Project, drawing close to a total of 80 interested researchers, students, and industry participants yearly. The 1st annual meeting of the Multistate Research Project was held in December 2012 in conjunction with the Conference of Research Workers and Animal Diseases in Chicago. The 2nd annual meeting of the Multistate Research Project was held in October 2013 in conjunction with The United States Animal Health Association (USAHA) and The American Association of Veterinary Laboratory Diagnosticians (AAVLD) in San Diego, CA; and the 3rd annual meeting was held in association with the annual meeting of the US Animal Health Association (USAHA) and the American Association of Veterinary Laboratory Diagnosticians (AAVLD) at the Sheraton Crown Center Hotel in Kansas City, MO in October 2014.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Kuenstner JT, Chamberlin W, Naser SA, Collins MT, Dow CT, Aitken JM, Weg S,Telega G, John K, Haas D, Eckstein TM, Kali M, Welch C, Petrie T. Resolution of Crohn's disease and complex regional pain syndrome following treatment of paratuberculosis. World J Gastroenterol. 2015 Apr 7;21(13):4048-62. doi:10.3748/wjg.v21.i13.4048. PubMed PMID: 5852293; PubMed Central PMCID: PMC4385555.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lamont EA, Talaat AM, Coussens PM, Bannantine JP, Grohn YT, Katani R, Li LL,Kapur V, Sreevatsan S. Screening of Mycobacterium avium subsp. paratuberculosis mutants for attenuation in a bovine monocyte-derived macrophage model. Front Cell Infect Microbiol. 2014 Jun 30;4:87. doi: 10.3389/fcimb.2014.00087. eCollection 2014. PubMed PMID: 25072030; PubMed Central PMCID: PMC4075333.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Ghosh P, Steinberg H, Talaat AM. Virulence and immunity orchestrated by the global gene regulator sigL in Mycobacterium avium subsp. paratuberculosis. Infect Immun. 2014 Jul;82(7):3066-75. doi: 10.1128/IAI.00001-14. Epub 2014 May 5. PubMed PMID: 24799632; PubMed Central PMCID: PMC4097628.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Gurung RB, Begg DJ, Purdie AC, de Silva K, Bannantine JP, Whittington RJ. Lymphoproliferative and gamma interferon responses to stress-regulated Mycobacterium avium subsp. paratuberculosis recombinant proteins. Clin Vaccine Immunol. 2014 Jun;21(6):831-7. doi: 10.1128/CVI.00775-13. Epub 2014 Apr 2. PubMed PMID: 24695774; PubMed Central PMCID: PMC4054239.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Hines ME 2nd, Turnquist SE, Ilha MR, Rajeev S, Jones AL, Whittington L, Bannantine JP, Barletta RG, Gr�hn YT, Katani R, Talaat AM, Li L, Kapur V. Evaluation of novel oral vaccine candidates and validation of a caprine model of Johne's disease. Front Cell Infect Microbiol. 2014 Mar 4;4:26. doi: 10.3389/fcimb.2014.00026. eCollection 2014. PubMed PMID: 24624365; PubMed Central PMCID: PMC3941644.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Cooney MA, Steele JL, Steinberg H, Talaat AM. A murine oral model for Mycobacterium avium subsp. paratuberculosis infection and immunomodulation with Lactobacillus casei ATCC 334. Front Cell Infect Microbiol. 2014 Feb 5;4:11. doi: 10.3389/fcimb.2014.00011. eCollection 2014. PubMed PMID: 24551602; PubMed CentralPMCID: PMC3914629.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Murray HL, Yabsley MJ, Keel MK, Manning EJ, Wilmers TJ, Corn JL. Persistence of Mycobacterium avium subspecies paratuberculosis in endangered Florida Key deer and Key deer habitat. J Wildl Dis. 2014 Apr;50(2):349-53. doi: 10.7589/2013-04-081. Epub 2014 Feb 7. PubMed PMID: 24506424.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Bannantine JP, Li L, Mwangi M, Cote R, Raygoza Garay JA, Kapur V. Complete Genome Sequence of Mycobacterium avium subsp. paratuberculosis, Isolated from Human Breast Milk. Genome Announc. 2014 Feb 6;2(1). pii: e01252-13. doi:10.1128/genomeA.01252-13. PubMed PMID: 24503996; PubMed Central PMCID: PMC3916490.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Bannantine JP, Bermudez LE. No holes barred: invasion of the intestinal mucosa by Mycobacterium avium subsp. paratuberculosis. Infect Immun. 2013 Nov;81(11):3960-5. doi: 10.1128/IAI.00575-13. Epub 2013 Aug 12. Review. PubMed PMID: 23940208; PubMed Central PMCID: PMC3811823.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Bannantine JP, Li LL, Sreevatsan S, Kapur V. How does a Mycobacterium change its spots? Applying molecular tools to track diverse strains of Mycobacterium avium subspecies paratuberculosis. Lett Appl Microbiol. 2013 Sep;57(3):165-73. doi: 10.1111/lam.12109. Epub 2013 Jun 19. PubMed PMID: 23721475.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Bradner L, Robbe-Austerman S, Beitz DC, Stabel JR. Chemical decontamination with N-acetyl-L-cysteine-sodium hydroxide improves recovery of viable Mycobacterium avium subsp. paratuberculosis organisms from cultured milk. J Clin Microbiol. 2013 Jul;51(7):2139-46. doi: 10.1128/JCM.00508-13. Epub 2013 May 1. PubMed PMID: 23637290; PubMed Central PMCID: PMC3697694.


Progress 02/15/13 to 02/14/14

Outputs
Target Audience: JDIP targets the following audiences: scientists, veterinarians, producers, regulatory agencies, educators, policy makers, and the general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? The 2nd annual meeting of the Multistate Research Project was held on October 19, 2013 in conjunction with The United States Animal Health Association (USAHA) and The American Association of Veterinary Laboratory Diagnosticians (AAVLD) in San Diego, CA. This year’s conference drew close to a total of 70 interested researchers, students, and industry participants. The Multistate Initiative Mycobacterial Diseases in Animals (MDA) has been active and has recently joined with 35 other scientific and animal agriculture focused organizations in urging Congress to pass a five-year farm bill. How have the results been disseminated to communities of interest? Results have been distributed through peer-reviewed publications, presentations at national and international conferences, extension meetings, communications in the lay press and radio shows, and through our web-site. What do you plan to do during the next reporting period to accomplish the goals? Continue our efforts to transition to the MDA program, and expand opportunities for additional extramural support.

Impacts
What was accomplished under these goals? JDIP has been able to facilitate research and education by focusing on developing new strategies to manage, prevent, and control JD disease. JDIP supports an inter-disciplinary communication among scientists, producers, and industry partners through our workshops, newsletters, and weekly conference calls. The efforts of JDIP scientists have led to the creation of new knowledge and the development a translational pipeline of new diagnostic tests, vaccine candidates together with the formulation of an outstanding education and training program. Our research and developments have resulted in (1) Application of an established 3-step framework of a Bayesian mathematical model of infection in data; (2) Establishment of benchmarks for diagnostic assays and usage of STandards for Reporting of Animal Diagnostic Accuracy Studies – paratuberculosis (STRADAS) guidelines for transparent and complete reporting, and continued research on the newly identified MAP specific monoclonal antibody, 17A2 that can for the first time differentiate MAP from other Mycobacterium; (3) Continued development on understanding of biology and pathogenesis of mycobacterial diseases in animals by emphasizing our understanding of paratuberculosis pathogenesis from the descriptive level to the mechanistic level; (4) Development of a better understanding of the molecular mechanisms of infection with emphasis on the role of super-shedders; (5) Development of new generations of vaccines for mycobacterial diseases in animals and identification of key genomics and proteomics candidates, vaccine efficacy studies; (6) Development on the extension, outreach, and development and distribution of education and outreach materials to stakeholders; (7) Establishment of a sample repository from JDIP samples for further testing and evaluation; (8) Finally, to expands the JDIP model to include bovine tuberculosis, JDIP investigators helped develop a new multistate initiative (Mycobacterial Diseases in Animals) MDA MI. The MDA seek to provide information and tools to producers and make science- based recommendations to policy makers, and importantly, provide a platform for collaborative research and the sharing of intellectual and physical resources amongst scientists, industry and government leaders. The 2nd annual meeting of the Multistate Research Project was held on October 19, 2013 in conjunction with The United States Animal Health Association (USAHA) and The American Association of Veterinary Laboratory Diagnosticians (AAVLD) in San Diego, CA. This year’s conference drew close to a total of 70 interested researchers, students, and industry participants. The Multistate Initiative Mycobacterial Diseases in Animals (MDA) has been active and has recently joined with 35 other scientific and animal agriculture focused organizations in urging Congress to pass a five-year farm bill.

Publications

  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Fecteau ME, Hovingh E, Whitlock RH, Sweeney RW (2013) Persistence of Mycobacterium avium subsp. paratuberculosis in soil, crops, and ensiled feed following manure spreading on infected dairy farms. Can Vet J 54:1083-1085.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Gurung RB, Begg DJ, Purdie AC, Bannantine JP, Whittington RJ (2013) Antigenicity of recombinant maltose binding protein-Mycobacterium avium subsp. paratuberculosis fusion proteins with and without factor Xa cleaving. Clin Vaccine Immunol 20:1817-1826.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Hines ME, 2nd, Turnquist SE, Ilha MR, Rajeev S, Jones AL, et al. (2014) Evaluation of novel oral vaccine candidates and validation of a caprine model of Johne's disease. Front Cell Infect Microbiol 4:26.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Hughes V, Denham S, Bannantine JP, Chianini F, Kerr K, et al. (2013) Interferon gamma responses to proteome-determined specific recombinant proteins: potential as diagnostic markers for ovine Johne's disease. Vet Immunol Immunopathol 155:197-204.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Khalifeh MS, Stabel JR (2013) Clinical disease upregulates expression of CD40 and CD40 ligand on peripheral blood mononuclear cells from cattle naturally infected with Mycobacterium avium subsp. paratuberculosis. Clin Vaccine Immunol 20:1274-1282.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Knust B, Patton E, Ribeiro-Lima J, Bohn JJ, Wells SJ (2013) Evaluation of the effects of a killed whole-cell vaccine against Mycobacterium avium subsp paratuberculosis in 3 herds of dairy cattle with natural exposure to the organism. J Am Vet Med Assoc 242:663-669.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Koop G, Collar CA, Toft N, Nielen M, van Werven T, et al. (2013) Risk factors for subclinical intramammary infection in dairy goats in two longitudinal field studies evaluated by Bayesian logistic regression. Prev Vet Med 108:304-312.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Kuo CJ, Ptak CP, Hsieh CL, Akey BL, Chang YF (2013) Elastin, a novel extracellular matrix protein adhering to mycobacterial antigen 85 complex. J Biol Chem 288:3886-3896.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lamont EA, Janagama HK, Ribeiro-Lima J, Vulchanova L, Seth M, et al. (2014) Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection. J Clin Microbiol 52:536-543.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Lamont EA, Xu WW, Sreevatsan S (2013) Host-Mycobacterium avium subsp. paratuberculosis interactome reveals a novel iron assimilation mechanism linked to nitric oxide stress during early infection. BMC Genomics 14:694.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Leite FL, Stokes KD, Robbe-Austerman S, Stabel JR (2013) Comparison of fecal DNA extraction kits for the detection of Mycobacterium avium subsp. paratuberculosis by polymerase chain reaction. J Vet Diagn Invest 25:27-34.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Li L, Gardner I, Rose R, Jamei M (2014) Incorporating Target Shedding Into a Minimal PBPK-TMDD Model for Monoclonal Antibodies. CPT Pharmacometrics Syst Pharmacol 3:e96.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Lu Z, Mitchell RM, Smith RL, Karns JS, van Kessel JA, et al. (2013) Invasion and transmission of Salmonella Kentucky in an adult dairy herd using approximate Bayesian computation. BMC Vet Res 9:245
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Lu Z, Schukken YH, Smith RL, Grohn YT (2013) Using vaccination to prevent the invasion of Mycobacterium avium subsp. paratuberculosis in dairy herds: a stochastic simulation study. Prev Vet Med 110:335-345.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Malley TJ, Stasiewicz MJ, Grohn YT, Roof S, Warchocki S, et al. (2013) Implementation of statistical tools to support identification and management of persistent Listeria monocytogenes contamination in smoked fish processing plants. J Food Prot 76:796-811.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: McNamara M, Tzeng SC, Maier C, Wu M, Bermudez LE (2013) Surface-exposed proteins of pathogenic mycobacteria and the role of cu-zn superoxide dismutase in macrophages and neutrophil survival. Proteome Sci 11:45.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Pithua P, Espejo LA, Godden SM, Wells SJ (2013) Is an individual calving pen better than a group calving pen for preventing transmission of Mycobacterium avium subsp paratuberculosis in calves? Results from a field trial. Res Vet Sci 95:398-404.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Rosa FB, Roussey J, Coussens PM, Langohr IM (2013) Pathology in practice. Johne's disease. J Am Vet Med Assoc 242:1655-1657.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Rose SJ, Bermudez LE (2014) Mycobacterium avium biofilm attenuates mononuclear phagocyte function by triggering hyperstimulation and apoptosis during early infection. Infect Immun 82:405-412.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Schwabenlander MD, Culhane MR, Hall SM, Goyal SM, Anderson PL, et al. (2013) A case of chronic wasting disease in a captive red deer (Cervus elaphus). J Vet Diagn Invest 25:573-576.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Settles EW, Kink JA, Talaat A (2014) Attenuated strains of Mycobacterium avium subspecies paratuberculosis as vaccine candidates against Johne's disease. Vaccine 32:2062-2069.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Silva CA, Danelishvili L, McNamara M, Berredo-Pinho M, Bildfell R, et al. (2013) Interaction of Mycobacterium leprae with human airway epithelial cells: adherence, entry, survival, and identification of potential adhesins by surface proteome analysis. Infect Immun 81:2645-2659.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Smith RL, Tauer LW, Schukken YH, Lu Z, Grohn YT (2013) Minimization of bovine tuberculosis control costs in US dairy herds. Prev Vet Med 112:266-275.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Sorge US, Kurnick S, Sreevatsan S (2013) Detection of Mycobacterium avium subspecies paratuberculosis in the saliva of dairy cows: a pilot study. Vet Microbiol 164:383-386.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Sorge US, Molitor T, Linn J, Gallaher D, Wells SW (2013) Cow-level association between serum 25-hydroxyvitamin D concentration and Mycobacterium avium subspecies paratuberculosis antibody seropositivity: a pilot study. J Dairy Sci 96:1030-1037.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Souza C, Davis WC, Eckstein TM, Sreevatsan S, Weiss DJ (2013) Mannosylated lipoarabinomannans from Mycobacterium avium subsp. paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of Mycobacterium avium subsp. avium organisms. PLoS One 8:e75924.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Stabel JR, Waters WR, Bannantine JP, Palmer MV (2013) Disparate host immunity to Mycobacterium avium subsp. paratuberculosis antigens in calves inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii, and M. bovis. Clin Vaccine Immunol 20:848-857.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Volkova VV, Lu Z, Lanzas C, Grohn YT (2013) Evaluating targets for control of plasmid-mediated antimicrobial resistance in enteric commensals of beef cattle: a modelling approach. Epidemiol Infect 141:2294-2312.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Volkova VV, Lu Z, Lanzas C, Scott HM, Grohn YT (2013) Modelling dynamics of plasmid-gene mediated antimicrobial resistance in enteric bacteria using stochastic differential equations. Sci Rep 3:2463.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Wadhwa A, Johnson RE, Mackintosh CG, Griffin JF, Waters WR, et al. (2013) Use of ethanol extract of Mycobacterium bovis for detection of specific antibodies in sera of farmed red deer (Cervus elaphus) with bovine tuberculosis. BMC Vet Res 9:256.


Progress 02/15/12 to 02/14/13

Outputs
OUTPUTS: Members of the JDIP consortium have continued to make notable progress in Johnes disease research, education and extension: Dr. Yrjo Grohn has been leading a group of investigators in the area of disease transmission and epidemiology. His team of scientists has developed and modified mathematical models and algorithms, and has implemented strategies to help improve diagnostic testing for MAP. Dr. John Bannantine has been leading a team of investigators developing diagnostics tests for MAP detection. Recently, the group has identified a MAP specific monoclonal antibody (17A2), which can differentiate MAP from other mycobacteria. This antibody has immense potential to help develop the next generation of diagnostic tests for JD, and is currently being evaluated in the laboratories of several JDIP investigators. Dr. Ray Sweeney and Ian Gardner groups continue to make progress in developing standardized approaches for the development of JD diagnostics. Samples have been collected from over 2000 cows located in herds in CA, PA, GA, TN, and MN, and a subset have been identified for retention in the repository. The samples are coded and frozen for storage. Results from these head-to-head comparisons are expected to be released during the summer of 2013. Dr. Murray Hines and his team have been leading Phase III of the JDIP Vaccine Efficacy Project, involving 5 mutant strains provided by JDIP investigators and 3 controls in groups of 10 goat kids. The goats were vaccinated and challenged with a K10-like strain. Monthly AGID, ELISA, fecal culture and PCR, periodic PPD skin testing with M. bovis, M. avium and MAP were performed. Data analyses from this tremendous investigation will be released by June 2013, and would provide a platform for community based evaluation of the most promising vaccine candidates and help shorten the timelines for translation of basic science discoveries into practical used. Drs. Ken Olson and Jeannette McDonald have worked on extension aspects of JDIP, and have provided education, certification and recertification for veterinarians in collaboration with VBJDCP, providing tools for producers, as well as effective communication between scientists, producers, industry partners, and USDA. Moreover, in collaboration with DHIA, three on-line modules covering milk sampling and ELISA testing for MAP were developed and are currently used by field and lab technicians. Tools for producer programs include an educational module on Johnes disease for dairy (English and Spanish), beef and small ruminant producers. JDIP also has had education information at producer meetings, including the World Dairy Expo, NIAG, USDA, and DMI events. JDIP also has a regular newsletter, and collaborates with ADSA by providing proceedings for the Searchable Proceedings of Animal Conferences. Other outreach efforts include the National Johnes Education Initiative, a website by the UW vet school (johnes.org), ADSA-ASAS Joint Annual Meeting, ICP, and USAHA/NJWG presentations among others. Representatives also attend meetings with USDA leader, partner organizations, and Congressional staff, as well as take part in priority setting at USDA stakeholder meetings. PARTICIPANTS: Dr. Vivek Kapur is the principal investigator for JDIP. The following members participate on the JDIP Executive Committee: John Bannantine, Ian Gradner, Yrio Grohn, Vivek Kapur, Ken Olson, Scott Wells, and Donald Lein. The following members participate on the JDIP Scientific Advisory Board: Beth Harris, Paul coussens, luiz Bermudez, Murry HinesII, Mike Paustian, Srinand Sreevatsan, Ynte Schukken, Yrio Grohn, Donald Lein, Elisabeth Patton, Ernest Hovingh, Ian Gardner, Jeannette McDonald, John Bannantine, Kenneth Olson, Suelee Robbe Austerman, Scott Wells, and Vivek Kapur. The following members participate on the JDIP External Advisory Board: Douwe Bakker, Michael Carter, Jamie Jonker, Cynthia Wolf, Donald Lein, Elisabeth Patton, Jamie Jonker, Ryan Ruppert, Michael Payne, David Kennedy, and Thomas Gomez. TARGET AUDIENCES: JDIP targets the following audiences: scientists, veterinarians, producers, regulatory agencies, educators, policy makers, and the general public. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Through collaborative research and shared intellectual and physical resources, JDIP has been able to facilitate research, education, and extension, and our efforts have led to the development of real-world solutions to mitigate losses associated with Johnes Disease (JD). With a focus on developing new strategies to manage, prevent, and control this disease, the efforts of JDIP scientists have led to the creation of new knowledge and the development a translational pipeline of new diagnostic tests, vaccine candidates together with the formulation of an outstanding education and training program. JDIP supports an inter-disciplinary communication among scientists, producers, and industry partners through our workshops, newsletters, regular conference calls, and a widely attended annual conference of JD researchers held in association with the ICP in February 2012 in Sydney, Australia, and in November 2012 in Chicago in association with the CRWAD. As stated in previous years, current year outcomes include: (1) development of epidemiologic mathematical models to help understanding of the dynamics of Mycobacterium avium subsp. paratuberculosis transmission on farm. The model also predicts and assists in the identification of critical control points in the transmission chain; (2) establishment of benchmarks for diagnostic assays with continuous validation of alternative sampling and testing strategies for detection of infected animals and herds; (3) development of protocols aimed at rapid detection of infected animals; (4) identification of the molecular mechanisms of infection, and the implications of spore formation related to super-shedders; (5) development and validation of vaccine candidates and evaluation of vaccine efficacy using standard approaches; (6) identification of key genomics and proteomics in MAP strains for development of the next generation of diagnostic tests and vaccines; and (7) continued development and widespread use of an on-line JD veterinary certification program. Finally, JDIP investigators helped develop a new multistate initiative (Mycobacterial Diseases in Animals) that expands the JDIP model to include bovine tuberculosis, and seeks to provide an important platform for collaborative research and the sharing of intellectual and physical resources amongst scientists, industry and government leaders, as well as for providing information and tools to producers and make science-based recommendations to policy makers. The 1st annual meeting of the Multistate Research Project was held on December 2, 2012 in conjunction with the Conference of Research Workers and Animal Diseases in Chicago. Since its inception, the Multistate Initiative has been active and has recently joined with 35 other scientific and animal agriculture focused organizations in urging Congress to pass a five-year farm bill. We also joined the AFRI Coalition Letter to Congress supporting the President FY 2013 budget proposal, and have joined 30 other industry and science organizations in the Animal Agriculture Coalition in a letter to congress relative to FY14 Agriculture Appropriations recommendations.

Publications

  • Allen, A. J., J. R. Stabel, S. Robbe-Austerman, K. T. Park, M. V. Palmer, G. M. Barrington, K. K. Lahmers, M. J. Hamilton, and W. C. Davis. 2012. Depletion of CD4 T lymphocytes at the time of infection with M. avium subsp. paratuberculosis does not accelerate disease progression. Vet Immunol Immunopathol. Oct 15, 2012. 149(3-4):286-91. http://dx.doi.org/10.1016/j.vetimm.2012.07.010. Epub Jul 27, 2012. PubMed PMID: 22898538.
  • Almeida, M. Q., M. F. Azevedo, P. Xekouki, E. L. Bimpaki, A. Horvath, M. T. Collins, L. P. Karaviti, G. S. Jeha, N. Bhattacharyya, C. Cheadle, T. Watkins, I. Bourdeau, M. Nesterova, and C. A. Stratakis. 2012. Activation of cyclic AMP signaling leads to different pathway alterations in lesions of the adrenal cortex caused by germline PRKAR1A defects versus those due to somatic GNAS mutations. J Clin Endocrinol Metab. Apr 2012. 97(4):E687-93. http://dx.doi.org/10.1210/jc.2011-3000. Epub Jan 18, 2012. PubMed PMID: 22259056.
  • Aly, S. S., R. J. Anderson, R. H. Whitlock, T. L. Fyock, S. C. McAdams, T. M. Byrem, J. Jiang, J. M. Adaska, and I. A. Gardner. 2012. Cost-effectiveness of diagnostic strategies to identify Mycobacterium avium subspecies paratuberculosis super-shedder cows in a large dairy herd using antibody enzyme-linked immunosorbent assays, quantitative real-time polymerase chain reaction, and bacterial culture. J Vet Diagn Invest. Sept 2012. 24(5):821-32. http://dx.doi.org/10.1177/1040638712452107. Epub Jul 17, 2012. PubMed, PMID: 22807510.
  • Arens, A. M., S. M. Puchalski, M. B. Whitcomb, R. Bell, I. A. Gardner, and S. M. Stover. 2013. Comparison of the use of scapular ultrasonography, physical examination, and measurement of serum biomarkers of bone turnover versus scintigraphy for detection of bone fragility syndrome in horses. J Am Vet Med Assoc. Jan 1, 2013. 242(1):76-85. http://dx.doi.org/10.2460/javma.242.1.76. PubMed PMID: 23234285.
  • Bannantine, J. P., C. K. Lingle, J. R. Stabel, K. X. Ramyar, B. L. Garcia, A. J. Raeber, P. Schacher, V. Kapur, and B. V. Geisbrecht. 2012. MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease. Clin Vaccine Immunol. July 2012. 19(7):1083-92. http://dx.doi.org/10.1128/CVI.00195-12. Epub May 16, 2012. PubMed PMID: 22593240; PubMed Central PMCID: PMC3393375.
  • Barlow, J. W., R. N. Zadoks, and Y. H. Schukken. 2013. Effect of lactation therapy on Staphylococcus aureus transmission dynamics in two commercial dairy herds. BMCVet Res. Feb 11, 2013. 9:28. http://dx.doi.org/10.1186/1746-6148-9-28. PubMed PMID: 23398676; PubMed Central PMCID: PMC3576258.
  • Stabel, J. R., A. Barnhill, J. P. Bannantine, Y. F. Chang, and M. A. Osman. 2012. Evaluation of protection in a mouse model after vaccination with Mycobacterium avium subsp. paratuberculois protein cocktails. Vaccine. Dec 17, 2012. 31(1):127-34. http://dx.doi.org/10.1016/j.vaccine.2012.10.090 . Epub Nov 6, 2012. PubMed PMID: 23137840.
  • Stiglbauer, K. E., K. M. Cicconi-Hogan, R. Richert, Y. H. Schukken, P. L. Ruegg, and M. Gamroth. 2013. Assessment of herd management on organic and conventional dairy farms in the United States. J Dairy Sci. Feb, 2013. 96(2):1290-300. http://dx.doi.org/10.3168/jds.2012-5845 . Epub Dec 6, 2012. PubMed PMID: 23219118.
  • Strawn, L. K., E. D. Fortes, E. A. Bihn, K. K. Nightingale, Y. T. Grohn, R. W. Worobo, M. Wiedmann, and P. W. Bergholz. 2013. Landscape and meteorological factors affecting prevalence of three food-borne pathogens in fruit and vegetable farms. Appl Environ Microbiol. Jan 2013. 79(2):588-600. http://dx.doi.org/10.1128/AEM.02491-12. Epub Nov 9. 2012. PubMed PMID: 23144137; PubMed Central PMCID: PMC3553790.
  • Sweeney, R. W., M. T. Collins, A. P. Koets, S. M. McGuirk, and A. J. Roussel. 2012. Paratuberculosis (Johne's disease) in cattle and other susceptible species. J Vet Intern Med. Nov-Dec 2012. 26(6):1239-50. http://dx.doi.org/10.1111/j.1939-1676.2012.01019.x. Epub Oct 28, 2012. PubMed PMID: 23106497.
  • Vallance, S. A., R. C. Entwistle, P. L. Hitchens, I. A. Gardner, and S. M. Stover. 2013. Case-control study of high-speed exercise history of Thoroughbred and Quarter Horse racehorses that died related to a complete scapular fracture. Equine Vet J. May 2013. 45(3):284-92. http://dx.doi.org/10.1111/j.2042-3306.2012.00644.x. Epub Dec 13, 2012. PubMed PMID: 23240828.
  • Van Kessel, J. A., J. S. Karns, D. R. Wolfgang, E. Hovingh, and Y. H. Schukken. 2012. Dynamics of Salmonella serotype shifts in an endemically infected dairy herd. Foodborne Pathog Dis. Apr 2012. 9(4):319-24. http://dx.doi.org/10.1089/fpd.2011.1054. Epub Feb 2, 2012. PubMed PMID: 22300223.
  • Volkova, V. V., C. Lanzas, Z. Lu, and Y. T. Grohn. 2012. Mathematical model of plasmid-mediated resistance to ceftiofur in commensal enteric Escherichia coli of cattle. PLoS One. 7(5):e36738. http://dx.doi.org/10.1371/journal.pone.0036738. Epub May 16, 2012. PubMed PMID: 22615803; PubMed Central PMCID: PMC3353932.
  • Vortmeyer, A. O., S. Glsker, G. U. Mehta, M. S. Abu-Asab, J. H. Smith, Z. Zhuang , M. T. Collins, and E. H. Oldfield. 2012. Somatic GNAS mutation causes widespread and diffuse pituitary disease in acromegalic patients with McCune-Albright syndrome. J Clin Endocrinol Metab. Jul 2012. 97(7):2404-13. http://dx.doi.org/10.1210/jc.2012-1274. Epub May 7, 2012. PubMed PMID: 22564667.
  • Wadhwa, A., J. P. Bannantine, T. M. Byrem, T. L. Stein, A. M. Saxton, C. A. Speer, and S. Eda. 2012. Optimization of serum EVELISA for milk testing of Johne's disease. Foodborne Pathog Dis. Aug 2012. 9(8):749-54. http://dx.doi.org/10.1089/fpd.2011.1115. Epub Jul 30, 2012. PubMed PMID: 22845627.
  • Ward, S. K., J. A. Heintz, R. M. Albrecht, and A. M. Talaat. 2012. Single-Cell Elemental Analysis of Bacteria: Quantitative Analysis of Polyphosphates in Mycobacterium tuberculosis. Front Cell Infect Microbiol. 2:63. http://dx.doi.org/10.3389/fcimb.2012.00063. Epub May 24, 2012. PubMed PMID: 22919654; PubMed Central PMCID: PMC3417655.
  • Watters, R. D., N. Schuring, H. N. Erb, Y. H. Schukken, and D. M. Galton. 2012. The effect of premilking udder preparation on Holstein cows milked 3 times daily. J Dairy Sci. Mar 2012. 95(3):1170-6. PubMed PMID: 22365201.
  • Waybrant, B., T. R. Pearce, P. Wang, S. Sreevatsan, and E. Kokkoli. 2012. Development and characterization of an aptamer binding ligand of fractalkine using domain targeted SELEX. Chem Commun (Camb). Oct 14, 2012. 48(80):10043-5. http://dx.doi.org./10.1039/c2cc34217k. PubMed PMID: 22936337.
  • Wongphatcharachai, M., P. Wang, S. Enomoto, R. J. Webby, M. R. Gramer, A. Amonsin, and S. Sreevatsan. 2013. Neutralizing DNA aptamers against swine influenza H3N2 viruses. J Clin Microbiol. Jan 2013. 51(1):46-54. http://dx.doi.org./10.1128/JCM.02118-12. Epub Oct17, 2012. PubMed PMID: 23077124; PubMed Central PMCID: PMC3536225.
  • Yavuz, S., W. F. Simonds, L. S. Weinstein, M. T. Collins, E. Kebebew, N. Nilubol, G. Q. Phan, S. K. Libutti, A. T. Remaley, M. Van Deventer, and S. J. Marx. 2012. Sleeping parathyroid tumor: rapid hyperfunction after removal of the dominant tumor. J Clin Endocrinol Metab. 2012 Jun;97(6):1834-41. http://dx.doi.org./10.1210/jc.2011-3030. Epub Apr 16, 2012. PubMed PMID: 22508712; PubMed Central PMCID: PMC3387414.
  • Zhao, W., E. Petit, R. I. Gafni, M. T. Collins, P. G. Robey, M. Seton, K. K. Miller, and M. Mannstadt. 2013. Mutations in NOTCH2 in patients with Hajdu-Cheney syndrome. Osteoporos Int. Feb 7, 2013. PubMed PMID: 23389697.
  • Lee, J. S., E. J. FitzGibbon, Y. R. Chen, H. J. Kim, L. R. Lustig, S. O. Akintoye, M. T. Collins, and L. B. Kaban. 2012. Clinical guidelines for the management of craniofacial fibrous dysplasia. Orphanet J Rare Dis. May 24, 2012. 7 Suppl 1:S2. http://dx.doi.org/10.1186/1750-1172-7-S1-S2. Epub May 24, 2012. PubMed PMID: 22640797; PubMed, Central PMCID: PMC3359960.
  • Lee, P., R. J. Brychta, M. T. Collins, J. Linderman, S. Smith, P. Herscovitch, C. Millo, K. Y. Chen, and F. S. Celi. 2012. Cold-activated brown adipose tissue is an independent predictor of higher bone mineral density in women. Osteoporos Int. Apr 2013. 24(4):1513-8. http://dx.doi.org/10.1007/s00198-012-2110-y. Epub Aug 14, 2012. PubMed PMID: 22890364.
  • Leite, F. L., K. D. Stokes, S. Robbe-Austerman, and J. R. Stabel. 2013. Comparison of fecal DNA extraction kits for the detection of Mycobacterium avium subsp. paratuberculosis by polymerase chain reaction. J Vet Diagn Invest. Jan 2013. 25(1):27-34. http://dx.doi.org/10.1177/1040638712466395. Epub Nov 19, 2012. PubMed PMID: 23166181.
  • Lim, A., J. P. Steibel, P. M. Coussens, D. L. Grooms, and S. R. Bolin. 2012. Differential gene expression segregates cattle confirmed positive for bovine tuberculosis from antemortem tuberculosis test-false positive cattle originating from herds free of bovine tuberculosis. Vet Med Int. 2012;2012:192926. http://dx.doi.org/10.1155/2012/192926. Epub Jun 4, 2012. PubMed PMID: 22701814; PubMed Central PMCID: PMC3373196.
  • Lombard, J. E., I. A. Gardner, S. R. Jafarzadeh, C. P. Fossler, B. Harris, R. T. Capsel, B. A. Wagner, and W. O. Johnson. 2013. Herd-level prevalence of Mycobacterium avium subsp. paratuberculosis infection in United States dairy herds in 2007. Prev Vet Med. Feb 1, 2013. 108(2-3):234-8. http://dx.doi.org/10.1016/j.prevetmed.2012.08.006. Epub Sep 12, 2012. PubMed PMID: 22979969.
  • Mayo, C. E., I. A. Gardner, B. A. Mullens, C. M. Barker, A. C. Gerry, A. J. Guthrie, and N. J. MacLachlan. 2012. Anthropogenic and meteorological factors influence vector abundance and prevalence of bluetongue virus infection of dairy cattle in California. Vet Microbiol. Mar 23, 2012. 155(2-4):158-64. http://dx.doi.org/10.1016/j.vetmic.2011.08.029 . Epub Sep 3, 2011. PubMed PMID: 21930352.
  • Mayo, C. E., B. A. Mullens, A. C. Gerry, C. M. Barker, P. P. Mertens, S. Maan, N. Maan, I. A. Gardner, A. J. Guthrie, and N. J. MacLachlan. 2012. The combination of abundance and infection rates of Culicoides sonorensis estimates risk of subsequent bluetongue virus infection of sentinel cattle on California dairy farms. Vet Parasitol. Jun 8, 2012. 187(1-2):295-301. http://dx.doi.org/10.1016/j.vetpar.2012.01.004 . Epub Jan. 8, 2012. PubMed PMID: 22281150.
  • McNamara, M., S. C. Tzeng, C. Maier, L. Zhang, and L. E. Bermudez. 2012. Surface proteome of Mycobacterium avium subsp. hominissuis during the early stages of macrophage infection. Infect Immun. May 2012. 80(5):1868-80. http://dx.doi.org/10.1128/IAI.06151-11 . Epub Mar 5, 2012. PubMed PMID: 22392927; PubMed Central PMCID: PMC3347459.
  • McNamara, M., L. Danelishvili, and L. E. Bermudez. 2012. The Mycobacterium avium ESX-5 PPE protein, PPE25-MAV, interacts with an ESAT-6 family Protein, MAV_2921, and localizes to the bacterial surface. Microb Pathog. Apr. 2012. 52(4):227-38. http://dx.doi.org/10.1016/j.micpath.2012.01.004 . Epub Jan 15, 2012. PubMed PMID: 22265661.
  • Lu, Z., Y. H. Schukken, R. L. Smith, R. M. Mitchell, and Y. T. Grohn. 2013. Impact of imperfect Mycobacterium avium subsp. paratuberculosis vaccines in dairy herds: a mathematical modeling approach. Prev Vet Med. Feb 1, 2013. 108(2-3):148-58. http://dx.doi.org/10.1016/j.prevetmed.2012.08.001 . Epub Aug 22, 2012. PubMed PMID: 22921715.
  • Nesterova, G., M. C. Malicdan, K. Yasuda, T. Sakaki, T. Vilboux, C. Ciccone, R. Horst, Y. Huang, G. Golas, W. Introne, M. Huizing, D. Adams, C. F. Boerkoel, M. T. Collins, and W. A. Gahl. 2013. 1,25-(OH)2D-24 Hydroxylase (CYP24A1) Deficiency as a Cause of Nephrolithiasis. Clin J Am Soc Nephrol. 2013 Apr 2013. 8(4):649-657. Epub Jan 4, 2013. PubMed PMID: 23293122.
  • Minozzi, G., J. L. Williams, A. Stella, F. Strozzi, M. Luini, M. L. Settles, J. F. Taylor, R. H. Whitlock, R. Zanella, and H. L. Neibergs. 2012. Meta-analysis of two genome-wide association studies of bovine paratuberculosis. PLoS One. 7(3):e32578. http://dx.doi.org/10.1371/journal.pone.0032578 . Epub Mar 2, 2012. PubMed PMID: 22396781; PubMed Central PMCID: PMC3292576.
  • Molla, B., M. Byrne, M. Abley, J. Mathews, C. R. Jackson, P. Fedorka-Cray, S. Sreevatsan, P. Wang, and W. A. Gebreyes. 2012. Epidemiology and genotypic characteristics of methicillin-resistant Staphylococcus aureus strains of porcine origin. J Clin Microbiol. Nov 2012. 50(11):3687-93. http://dx.doi.org/10.1128/JCM.01971-12 . Epub Sep 12, 2012. PubMed PMID: 22972820; PubMed Central PMCID: PMC3486229.
  • Morton, J. M., R. J. McCoy, R. K. Kann, I. A. Gardner, and J. Meers. 2012. Validation of real-time polymerase chain reaction tests for diagnosing feline immunodeficiency virus infection in domestic cats using Bayesian latent class models. Prev Vet Med. Apr 1, 2012. 104(1-2):136-48. http://dx.doi.org/10.1016/j.prevetmed.2011.10.009 . Epub Nov 17, 2011. PubMed PMID: 22098681.
  • Oikonomou, G., V. S. Machado, C. Santisteban, Y. H. Schukken, and R. C. Bicalho. 2012. Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomic 16s rDNA. PLoS One. 2012. 7(10):e47671. http://dx.doi.org/10.1371/journal.pone.0047671 . Epub Oct 17, 2012.. PubMed PMID: 23082192; PubMed Central PMCID: PMC3474744.
  • Phillips, G. E., M. J. Lavelle, J. W. Fischer, J. J. White, S. J. Wells, and K. C. Vercauteren. 2012. A novel bipolar electric fence for excluding white-tailed deer from stored livestock feed. J Anim Sci. Nov 2012. 90(11):4090-7. http://dx.doi.org/10.2527/jas.2011-4619 . Epub Aug 2, 2012. PubMed PMID: 22859758.
  • Reksen, O., Y. T. Grohn, J. W. Barlow, and Y. H. Schukken. 2012. Transmission dynamics of intramammary infections with coagulase-negative staphylococci. J Dairy Sci. 2012 Sep 2012. 95(9):4899-910. http://dx.doi.org/10.3168/jds.2011-4675 . PubMed PMID: 22916894.
  • Reyner, L. A., S. J. Wells, V. Mortlock, and J. A. Horne. 2012. 'Post-lunch' sleepiness during prolonged, monotonous driving - effects of meal size. Physiol Behav. Feb 28, 2012. 105(4):1088-91. http://dx.doi.org/10.1016/j.physbeh.2011.11.025 . Epub Dec 6, 2011. PubMed PMID: 22155490.
  • Cummings, K. J., L. D. Warnick, M. A. Davis, K. Eckmann, Y. T. Grohn, K. Hoelzer, K. MacDonald, T. P. Root, J. D. Siler, S. M. McGuire, M. Wiedmann, E. M. Wright, S. M. Zansky, and T. E. Besser. 2012. Farm animal contact as risk factor for transmission of bovine-associated Salmonella subtypes. Emerg Infect Dis. Dec 2012. 18(12):1929-36. http://dx.doi.org/10.3201/eid1812.110831. PubMed PMID: 23171627; PubMed Central PMCID: PMC3557873.
  • Dogan, B., M. Rishniw, G. Bruant, J. Harel, Y. H. Schukken, and K. W. Simpson. 2012. Phylogroup and lpfA influence epithelial invasion by mastitis associated Escherichia coli. Vet Microbiol. Sep 14, 2012. 159(1-2):163-70. http://dx.doi.org/10.1016/j.vetmic.2012.03.033. Epub Mar 30, 2012. PubMed PMID: 22510704.
  • Donahue, M., S. M. Godden, R. Bey, S. Wells, J. M. Oakes, S. Sreevatsan, J. Stabel, and J. Fetrow. 2012. Heat treatment of colostrum on commercial dairy farms decreases colostrum microbial counts while maintaining colostrum immunoglobulin G concentrations. J Dairy Sci. May 2012. 95(5):2697-702. http://dx.doi.org/10.3168/jds.2011-5220. PubMed PMID: 22541498.
  • England, E. M., K. D. Fisher, S. J. Wells, D. A. Mohrhauser, D. E. Gerrard, and A. D. Weaver. 2012. Postmortem titin proteolysis is influenced by sarcomere length in bovine muscle. J Anim Sci. Mar 2012. 90(3):989-95. http://dx.doi.org/10.2527/jas.2011-4278. Epub Oct 7, 2011. PubMed PMID: 21984717.
  • Espejo, L. A., S. Godden, W. L. Hartmann, and S. J. Wells. 2012. Reduction in incidence of Johne's disease associated with implementation of a disease control program in Minnesota demonstration herds. J Dairy Sci. Jul 2012. 95(7):4141-52. http://dx.doi.org/10.3168/jds.2011-4550. PubMed PMID: 22720971.
  • Gafni, R. I., J. S. Brahim, P. Andreopoulou, N. Bhattacharyya, M. H. Kelly, B. A. Brillante, and J. C. Reynolds. 2012. Hypoparathyroidism induces marked changes in bone turnover and structure. J Bone Miner Res. Aug 2012. 27(8):1811-20. http://dx.doi.org/10.1002/jbmr.1627. PubMed PMID: 22492501; PubMed Central PMCID: PMC3399961.
  • Gara, N., X. Zhao, M. T. Collins, W. H. Chong, D. E. Kleiner, T. Jake Liang, M. G. Ghany, and J. H. Hoofnagle. 2012. Renal tubular dysfunction during long-term adefovir or tenofovir therapy in chronic hepatitis B. Aliment Pharmacol Ther. Jun 2012. 35(11):1317-25. http://dx.doi.org/10.1111/j.1365-2036.2012.05093.x. Epub Apr 16, 2012. PubMed PMID: 22506503; PubMed Central PMCID: PMC3443969.
  • Joshi, D., N. B. Harris, R. Waters, T. Thacker, B. Mathema, B. Krieswirth, and S. Sreevatsan. 2012. Single nucleotide polymorphisms in the Mycobacterium bovis genome resolve phylogenetic relationships. J Clin Microbiol. Dec 2012. 50(12):3853-61. http://dx.doi.org/10.1128/JCM.01499-12. Epub Sep 19, 2012. PubMed PMID: 22993186; PubMed Central PMCID: PMC3502966.
  • Ghosh, P., C. Hsu, E. J. Alyamani, M. M. Shehata, M. A. Al-Dubaib, A. Al-Naeem, M. Hashad, O. M. Mahmoud, K. B. Alharbi, K. Al-Busadah, A. M. Al-Swailem, and A. M. Talaat. 2012. Genome-wide analysis of the emerging infection with Mycobacterium avium subspecies paratuberculosis in the Arabian camels (Camelus dromedarius). PLoS One. 7(2):e31947. http://dx.doi.org/10.1371/journal.pone.0031947. Epub Feb 29, 2012. PubMed PMID: 22393374; PubMed Central PMCID: PMC3290536.
  • Godden, S. M., D. J. Smolenski, M. Donahue, J. M. Oakes, R. Bey, S. Wells, S. Sreevatsan, J. Stabel, and J. Fetrow. 2012. Heat-treated colostrum and reduced morbidity in preweaned dairy calves: results of a randomized trial and examination of mechanisms of effectiveness. J Dairy Sci. Jul 2012. 95(7):4029-40. http://dx.doi.org/10.3168/jds.2011-5275. PubMed PMID: 22720957.
  • Johnson, A. L., R. W. Sweeney, S. C. McAdams, and R. H. Whitlock. 2012. Quantitative real-time PCR for detection of the neurotoxin gene of Clostridium botulinum type B in equine and bovine samples. Vet J. Oct 2012. 194(1):118-20. http://dx.doi.org/10.1016/j.tvjl.2012.03.018. Epub Apr 24, 2012. PubMed PMID: 22537645.
  • Bartpho, T., T. Wongsurawat, S. Wongratanacheewin, A. M. Talaat, N. Karoonuthaisiri, and R. W. Sermswan. 2012. Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei. PLoS One. 2012;7(6):e37762. http://dx.doi.org/10.1371/journal.pone.0037762. Epub Jun 1, 2012. PubMed PMID: 22675491; PubMed Central PMCID: PMC3365882.
  • Bell, L. W., M. H. Ryan, R. G. Bennett, M. T. Collins, and H. J. Clarke. 2012. Growth, yield and seed composition of native Australian legumes with potential as grain crops. J Sci Food Agric. 2012 May 2012. 92(7):1354-61. http://dx.doi.org/10.1002/jsfa.4706. Epub Nov 14, 2011. PubMed, PMID: 22083564.
  • Bermudez, L. E., C. B. Inderlied, P. Kolonoski, C. B. Chee, P. Aralar, M. Petrofsky, T. Parman, C. E. Green, A. H. Lewin, W. Y. Ellis, and S. Young. 2012. Identification of (+)-erythro-mefloquine as an active enantiomer with greater efficacy than mefloquine against Mycobacterium avium infection in mice. Antimicrob Agents Chemother. Aug 2012. 56(8):4202-6. http://dx.doi.org/10.1128/AAC.00320-12. Epub May 21, 2012. PubMed PMID: 22615290; PubMed Central PMCID: PMC3421598.
  • Kabara, E. and P. M. Coussens. 2012. Infection of Primary Bovine Macrophages with Mycobacterium avium Subspecies paratuberculosis Suppresses Host Cell Apoptosis. Front Microbiol. 3:215. http://dx.doi.org/10.3389/fmicb.2012.00215. Epub Jul 20, 2012. PubMed PMID: 22833736; PubMed Central PMCID: PMC3400940.
  • Guthrie, A. J., N. J. Maclachlan, C. Joone, C. W. Lourens, C. T. Weyer, M. Quan, M. S. Monyai, and I. A. Gardner. 2013. Diagnostic accuracy of a duplex real-time reverse transcription quantitative PCR assay for detection of African horse sickness virus. J Virol Methods. Apr 2013. 189(1):30-5. http://dx.doi.org/10.1016/j.jviromet.2012.12.014. Epub Jan 3, 2013. PubMed PMID: 23291102.
  • Haran, K. P., S. M. Godden, D. Boxrud, S. Jawahir, J. B. Bender, and S. Sreevatsan. 2012. Prevalenceand characterization of Staphylococcus aureus, including methicillin-resistantStaphylococcus aureus, isolated from bulk tank milk from Minnesota dairy farms. JClin Microbiol. 2012 Mar;50(3):688-95. http://dx.doi.org/10.1128/JCM.05214-11. Epub Dec14, 2011. PubMed PMID: 22170937; PubMed Central PMCID: PMC3295154.
  • Heuer, C., R. M. Mitchell, Y. H. Schukken, Z. Lu, C. Verdugo, and P. R. Wilson. 2012. Modelling transmission dynamics of paratuberculosis of red deer under pastoral farming conditions. Prev Vet Med. Sep 1, 2012. 106(1):63-74. http://dx.doi.org/10.1016/j.prevetmed.2012.02.021. Epub Apr 3, 2012. PubMed PMID: 22480407.
  • Jeske, J. M., S. J. Spier, M. B. Whitcomb, N. Pusterla, and I. A. Gardner. 2013. Use of antibody titers measured via serum synergistic hemolysis inhibition testing to predict internal Corynebacterium pseudotuberculosis infection in horses. J Am Vet Med Assoc. Jan 1, 2013. 242(1):86-92. http://dx.doi.org/10.2460/javma.242.1.86. PubMed PMID: 23234286.
  • Kim, H. B., K. Borewicz, B. A. White, R. S. Singer, S. Sreevatsan, Z. I. Tu, and R. E. Isaacson. 2012. Microbial shifts in the swine distal gut in response to the treatment with antimicrobial growth promoter, tylosin. Proc Natl Acad Sci U S A. Sep 18, 2012. 109(38):15485-90. Epub Sep 6, 2012. PubMed PMID: 22955886; PubMed Central PMCID: PMC3458334.
  • Lamont, E. A., S. M. O'Grady, W. C. Davis, T. Eckstein, and S. Sreevatsan. 2012. Infection with Mycobacterium avium subsp. paratuberculosis results in rapid interleukin-1 release and macrophage transepithelial migration. Infect Immun. Sep 2012. 80(9):3225-35. http://dx.doi.org/10.1128/IAI.06322-11. Epub Jul 9, 2012. PubMed PMID: 22778093; PubMed Central PMCID: PMC3418758.
  • Bhattacharyya, N., M. Wiench, C. Dumitrescu, B. M. Connolly, T. H. Bugge, H. V. Patel, R. I. Gafni, N. Cherman, M. Cho, G. L. Hager, and M. T. Collins. 2012. Mechanism of FGF23 processing in fibrous dysplasia. J Bone Miner Res. May 2012. 27(5):1132-41. http://dx.doi.org/10.1002/jbmr.1546. PubMed PMID: 22247037.
  • Bhattacharyya, N., W. H. Chong, R. I. Gafni, and M. T. Collins. 2012. Fibroblast growth factor 23: state of the field and future directions. Trends Endocrinol Metab. Dec 2012. 23(12):610-8. http://dx.doi.org/10.1016/j.tem.2012.07.002. Epub 2012 Aug 24. PubMed PMID: 22921867; PubMed Central PMCID: PMC3502714.
  • Boyce, A. M., M. Glover, M. H. Kelly, B. A. Brillante, J. A. Butman, E. J. Fitzgibbon, C. C. Brewer, C. K. Zalewski, C. M. Cutler Peck, H. J. Kim, and M. T. Collins. 2013. Optic neuropathy in McCune-Albright syndrome: effects of early diagnosis and treatment of growth hormone excess. J Clin Endocrinol Metab. Jan 2013. 98(1):E126-34. http://dx.doi.org/10.1210/jc.2012-2111. Epub Oct 23, 2012. PubMed PMID: 23093488; PubMed Central PMCID: PMC3537097.
  • Boyce, A. M., W. H. Chong, T. H. Shawker, P. A. Pinto, W. M. Linehan, N. Bhattacharryya, M. J. Merino, F. R. Singer, and M. T. Collins. 2012. Characterization and management of testicular pathology in McCune-Albright syndrome. J Clin Endocrinol Metab. Sep 2012. 97(9):E1782-90. http://dx.doi.org/10.1210/jc.2012-1791. Epub Jun 28, 2012. PubMed PMID: 22745241; PubMed Central PMCID: PMC3431566.
  • Bannantine, J. P., C. W. Wu, C. Hsu, S. Zhou, D. C. Schwartz, D. O. Bayles, M. L. Paustian, D. P. Alt, S. Sreevatsan, V. Kapur, and A. M. Talaat. 2012. Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association. BMC Genomics. March 12, 2012. 13:89. http://dx.doi.org/10.1186/1471-2164-13-89. PubMed PMID: 22409516; PubMed Central PMCID: PMC3337245.
  • Boyce, A. M., W. H. Chong, J. Yao, R. I. Gafni, M. H. Kelly, C. E. Chamberlain, C. Bassim, N. Cherman, M. Ellsworth, J. Z. Kasa-Vubu, F. A. Farley, A. A. Molinolo, N. Bhattacharyya, and M. T. Collins. 2012. Denosumab treatment for fibrous dysplasia. J Bone Miner Res. Jul 2012. 27(7):1462-70. http://dx.doi.org/10.1002/jbmr.1603. PubMed PMID: 22431375; PubMed Central, PMCID: PMC3377825.
  • Chander, Y., N. Jindal, S. Sreevatsan, D. E. Stallknecht, and S. M. Goyal. 2012. Molecular and phylogenetic analysis of matrix gene of avian influenza viruses isolated from wild birds and live bird markets in the USA. Influenza Other Respi Viruses. Sep 8, 2012. http://dx.doi.org/10.1111/irv.12003. [Epub ahead of print] PubMed PMID: 22958470.
  • Chen, J. W., S. M. Faisal, S. Chandra, S. P. McDonough, M. A. Moreira, J. Scaria, C. F. Chang, J. P. Bannantine, B. Akey, and Y. F. Chang. 2012. Immunogenicity and protective efficacy of the Mycobacterium avium subsp. paratuberculosis attenuated mutants against challenge in a mouse model. Vaccine. Apr 19, 2012. 30(19):3015-25. http://dx.doi.org/10.1016/j.vaccine.2011.11.029. Epub Nov 19, 2011. PubMed PMID: 22107851.
  • Cho, J., L. W. Tauer, Y. H. Schukken, M. I. Gomez, R. L. Smith, Z. Lu, and Y. T. Grohn. 2012. Economic analysis of Mycobacterium avium subspecies paratuberculosis vaccines in dairy herds. J Dairy Sci. Apr 2012. 95(4):1855-72. http://dx.doi.org/10.3168/jds.2011-4787. PubMed PMID: 22459833.
  • Coussens, P. M., S. Sipkovsky, B. Murphy, J. Roussey, and C. J. Colvin. 2012. Regulatory T cells in cattle and their potential role in bovine paratuberculosis. Comp Immunol Microbiol Infect Dis. May 2012. 35(3):233-9. http://dx.doi.org/10.1016/j.cimid.2012.01.004. Epub Jan 27, 2012. Review. PubMed PMID: 22285689.
  • Crafford, J. E., C. W. Lourens, I. A. Gardner, N. J. Maclachlan, and A. J. Guthrie. 2012. Passive transfer and rate of decay of maternal antibody against African horse sickness virus in South African Thoroughbred foals. Equine Vet J. Nov 7, 2012. http://dx.doi.org/10.1111/evj.12015. PubMed PMID: 23294121.
  • Cummings, K. J., L. D. Warnick, Y. T. Grohn, K. Hoelzer, T. P. Root, J. D. Siler, S. M. McGuire, E. M. Wright, S. M. Zansky, and M. Wiedmann. 2012. Clinical features of human salmonellosis causedby bovine-associated subtypes in New York. Foodborne Pathog Dis. Sep 2012. 9(9):796-802. http://dx.doi.org/10.1089/fpd.2012.1158. Epub 2012 Aug 7. PubMed PMID: 22870888; PubMed Central PMCID: PMC3497887.
  • Chong, W. H., P. Andreopoulou, C. C. Chen, J. Reynolds, L. Guthrie, M. Kelly, R. I. Gafni, N. Bhattacharyya, A. M. Boyce, D. El-Maouche, D. Ovejero Crespo, R. Sherry, R. Chang, F. M. Wodajo, G. B. Kletter, A. Dwyer, and M. T. Collins. 2013. Tumor localization and biochemical response to cure in tumor-induced osteomalacia. J Bone Miner Res. Jan 29, 2013. http://dx.doi.org/10.1002/jbmr.1881. [Epub ahead of print] PubMed PMID: 23362135.
  • Collins, M. T. 2013. Canine inflammatory bowel disease: current and prospective biomarkers for diagnosis and management. Compend Contin Educ Vet. 35(3):E1-7. PubMed PMID: 23532922.
  • Collins, M. T., F. R. Singer, and E. Eugster. 2012. McCune-Albright syndrome and the extraskeletal manifestations of fibrous dysplasia. Orphanet J Rare Dis. May 24, 2012. 7 Suppl 1:S4. http://dx.doi.org/10.1186/1750-1172-7-S1-S4. Epub May 24, 2012. Review. PubMed, PMID: 22640971; PubMed Central PMCID: PMC3359955.


Progress 02/15/11 to 02/14/12

Outputs
OUTPUTS: JDIP investigators continue to make considerable progress despite significant delays (of up to 8 months or more in several cases) in processing of awards by the USDA. Some examples of the impressive progress by JDIP investigators in the area of epidemiology, diagnostics, vaccine development, and education and extension programming include: Dr. Yrjo Grohn's group working with several collaborators has developed mathematical models, algorithms, and testing strategies that help improve diagnostic testing for MAP and better understand disease transmission and epidemiology. Dr. John Bannantine's group has been working with several other JDIP investigators and has identified MAP antigens that are reactive with monoclonal antibodies that have been previously prepared as highly specific and sensitive diagnostic tools. This involves both screening a phage expression library and using the protein array developed in the laboratory and will help in developing the next generation of diagnostic tests for JD and MAP infection. Dr. Ray Sweeneys' group in collaboration with several other JDIP investigators (Srinand Sreevatsan, Ernest Hovingh, Murray Hines and Ian Gardner) continues to make progress in JD diagnostics. Samples have been collected from herds in CA, PA, GA, TN, and MN. Close to 2000 cows have been sampled, and just over the target number of 900 (500 from infected herds, 400 from Status 4 "uninfected" herds) have been identified for retention in the repository. The samples are coded, separated into individual testing aliquots, and frozen for storage. Dr. Murray Hines group in collaboration with several other JDIP investigators continues to make good progress on the final phase (Phase 3) of the JDIP vaccine trial. Studies have been initiated to rank the top vaccine candidates identified from Phase 2 in 80 goat kids in groups of 10 each, with five (5) vaccine groups and three (3) controls similar to those in the recently concluded mouse trials. Drs. Jeannette McDonald and Ken Olson, together with other JDIP investigators continue to make a major impact on the Education/Outreach project. Their main goal has been the development of the Johne's Disease Risk Assessment for Producers (JD-RAP). This interactive, web-based simulation has been designed to help producers explore the economic costs of low and high prevalence Johne's disease, including the role of testing and management. The current structure for the module was selected as a result of finding in JDIP "National Dairy Producer Survey," that highlighted the fact that many producers don't fully understand the impact of management options available for use with testing and culling. Therefore, it was decided to use the Risk Assessment as a framework for producers to learn the importance, and explore the impact, of management and testing changes. Initial funding for the effort was provided by JDIP and additional funding was provided by the National Milk Producers Federation (NMPF), a major dairy producer organization in partnership with JDIP for completing the effort and in making it widely available to producers. It is anticipated that the final version will be available by early summer 2012. PARTICIPANTS: Dr. Vivek Kapur is the principal investigator for JDIP. The following members participate on the JDIP Executive Committee: John Bannantine, Ian Gardner, Yrjo Grohn, Vivek Kapur, Ken Olson, and Scott Wells. The following members participate on the JDIP Scientific Advisory Board: Luiz Bermudez, Paul Coussens, Suelee Robbe-Austerman, Murray Hines II, Ernest Hovingh, Jeannette McDonald, Michael Paustian, Ynte Schukken, and Srinand Sreevatsan. The following members participate on the JDIP External Advisory Board: Douwe Bakker, Marcel Behr, Terry Bowersock, Michael Carter, Thomas Gomez, Lawrence Hutchinson, Jamie Jonker, David Kennedy, Donald Lein, Carol Nacy, Elisabeth Patton, Michael Payne, Alex Raeber, Ryan Ruppert, Cynthia Wolf, and Zuidhof, Sjoert. Other funded co-investigators include: Randall J. Anderson, Sandra Godden, Lee Jones, Bob Patrick, Raymond Sweeney, and Brian Whitlock TARGET AUDIENCES: JDIP targets the following audiences: scientists, veterinarians, producers, regulatory agencies, educators, policy makers, and the general public. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
JDIP facilitates Johne's disease (JD) research, education, and extension in a manner that would not have usually been available through traditional USDA or other funding agency sources. Our research endeavors have focused on strategies to manage, prevent and control the disease; a translational pipeline of new diagnostic tests; vaccine candidates; and the formulation of an outstanding education and training program. JDIP also offers the community strong communication outlets and extension plans through our workshops, newsletters, standard conference calls, and a widely attended and very successful annual conference of JD researchers called "JDIP at JAM," which was held in association with the Joint Annual Meeting of the Animal Societies in July 2011 in New Orleans, Louisiana. JDIP also held a meeting in association with the 11th International Colloquium on Paratuberculosis in February 2012 in Sydney, Australia. Over the course of the program, through the direct achievements of JDIP members and investigators, our research and developments have resulted in (1) continued improvement of our understanding of Mycobacterium avium subsp. paratuberculosis (MAP) on-farm transmission dynamics that is helping identify critical control points in the transmission chain; (2) continued development and validation of alternative sampling and testing strategies for detection of infected animals and herds that are being adopted by the national voluntary control program for JD; (3) development and evaluation of standard laboratory protocols for MAP culture and PCR for reducing timelines for rapid detection of infected animals; (4) development of a better understanding of the molecular mechanisms of infection; (5) development of standard approaches for the validation of vaccine candidates and evaluation of vaccine efficacy; (6) identification of key genes, proteins and lipids in MAP for development of the next generation of diagnostic tests and vaccines; (7) continued development and widespread use of an on-line JD veterinary certification program; and, (8) development of community resources including MAP isolates, serum samples and other clinical material for the development and validation of diagnostic tests, genomic microarrays, recombinant proteins, and mutant strain banks of MAP for identification of potential vaccine candidates. JDIP facilitates collaborations by hosting meetings for both scientists and stakeholders and helps improve animal health through focused research, extension and education activities on JD. Overall, JDIP continues to meet and surpass its project objectives.

Publications

  • Whittington, R. J., I. B. Marsh, V. Saunders, I. R. Grant , R. Juste, I. A. Sevilla, E. J. Manning, and R. H. Whitlock. 2011 Culture Phenotypes of Genomically and Geographically Diverse Mycobacterium avium subsp. paratuberculosis Isolates from Different Hosts. J Clin Microbiol. May; 49(5):1822-30.
  • Raizman, E. A., M. Y. Habteselassie, C. C. Wu, T. L. Lin, M. Negron, and R. F. Turco. 2011. Leaching of Mycobacterium avium Subsp paratuberculosis in Soil under In Vitro Conditions. Vet Med Int. 2011; 2011:506239.
  • Raizman, E. A., L. A. Espejo, and S. J. Wells. 2011. Long-Term Survival of Mycobacterium avium subsp. paratuberculosis in Fecal Samples Obtained from Naturally Infected Cows and Stored at -18 degrees C and -70 degrees C. Vet Med Int. 2011; 2011:341691.
  • Smith, R. L., Y. H. Schukken, A. K. Pradhan, J. M. Smith, R. H. Whitlock, J. S. Van Kessel, D. R. Wolfgang, and Y. T. Grohn. 2011. Environmental contamination with Mycobacterium avium subsp. paratuberculosis in endemically infected dairy herds. Prev Vet Med. 2011 Oct; 102(1):1-9.
  • Allen, A. J., K. T. Park, G. M. Barrington, K. K. Lahmers, G. S. Abdellrazeq, H. M. Rihan, S. Sreevatsan, C. Davies, M. J. Hamilton, and W. C. Davis. 2011. Experimental infection of a bovine model with human isolates of Mycobacterium avium subsp. paratuberculosis. Vet Immunol Immunopathol 2011 Jun 15; 141(3-4):258-66.
  • Bannantine, J. P., J. R. Stabel, E. A. Lamont, R. E. Briggs, and S. Sreevatsan. 2011. Monoclonal Antibodies Bind A SNP-Sensitive Epitope that is Present Uniquely in Mycobacterium avium Subspecies Paratuberculosis. Front Microbiol. 2:163.
  • Smith, R. L., Schukken, Y. H., Pradhan, A. K., Smith, J. M., Whitlock, R. H., Van Kessel, J. S., Wolfgang, D. R., Grohn, Y. T. 2011. Environmental contamination with Mycobacterium avium subsp. paratuberculosis in endemically infected dairy herds. Preventive Veterinary Medicine 2011 Oct 1; 102(1):1-9.
  • Stabel, J. R. and S. Robbe-Austerman. 2011. Early Immune Markers Associated with Mycobacterium avium subsp. paratuberculosis Infection in a Neonatal Calf Model. Clin Vaccine Immunol. 2011 Mar; 18(3):393-405.
  • Stabel, J. R., J. P. Bannantine, S. Eda, and S. Robbe-Austerman. 2011. Induction of B Cell Responses upon Experimental Infection of Neonatal Calves with Mycobacterium avium subsp. paratuberculosis. Clin Vaccine Immunol. 2011 Jul; 18(7):1139-49.
  • Teose, M. and K. Ahmadizadeh, et al. 2011 Embedding system dynamics in agent based models for complex adaptive systems. Int. Joint Conf. Artificial Intelligence. AAAI Press Proceedings 7:19-22.
  • Wang, C., B. W. Turnbull, S. S. Nielsen, Y. T. Grohn. 2011. Bayesian analysis of longitudinal Johnes disease diagnostic data without a gold standard test. Journal of Dairy Science 2011 94:2320-2328.
  • Fisher, C. A., E. K. Bhattarai, J. B. Osterstock, S. E. Dowd, P. M. Seabury, M. Vikram, R. H. Whitlock, Y. H. Schukken, R. D. Schnabel, J. F. Taylor, J. E. Womack, and C. M. Seabury. 2011. Evolution of the Bovine TLR Gene Family and Member Associations with Mycobacterium avium Subspecies paratuberculosis Infection. PLoS One 6(11):e27744.
  • Gardner, I. A., S. S. Nielsen, R. J. Whittington. 2011. Consensus-based reporting standards for diagnostic test accuracy studies for paratuberculosis in ruminants. Prev Vet Med. 2011 Aug 1; 101(1-2):18-34.
  • Hsu, C. Y., C. W. Wu, and A. M. Talaat. 2011. Genome-Wide Sequence Variation among Mycobacterium avium Subspecies paratuberculosis Isolates: A Better Understanding of Johnes Disease Transmission Dynamics. Front Microbiol. 2:236.
  • Kirkpatrick, B. W., X. Shi, G. E. Shook, M. T. Collins. 2011. Genetic markers associated with susceptibility to paratuberculosis in Holsteins. Animal Genetics 42:149-160.
  • Nielsen, S. S., N. Toft, and I. A. Gardner. 2011. Structured approach to design of diagnostic test evaluation studies for chronic progressive infections in animals. Vet Microbiol. 150(1-2):115-25.
  • Park, K. T., A. J. Allen, J. P. Bannantine, K. S. Seo, M. J. Hamilton, G. S. Abdellrazeq, H. M. Rihan, A. Grimm, and W. C. Davis. 2011. Evaluation of two mutants of Mycobacterium avium subsp. paratuberculosis as candidates for a live attenuated vaccine for Johne's disease. Vaccine. 2011 Jun 24; 29(29-30):4709-19.
  • Pithua, P., S. J. Wells, S. M. Godden, and J. R. Stabel. 2011. Evaluation of the association between fecal excretion of Mycobacterium avium subsp paratuberculosis and detection in colostrum and on teat skin surfaces of dairy cows. J Am Vet Med Assoc. 2011 Jun 1; 238(11):1440.
  • Pradhan, A. K., R. M. Mitchell, A. J. Kramer, M. J. Zurakowski, T. L. Fyock, R. H. Whitlock, J. M. Smith, E. Hovingh, J. A. Van Kessel , J. S. Karns, and Y. H. Schukken. 2011. Molecular Epidemiology of Mycobacterium avium subsp. paratuberculosis in a Longitudinal Study of Three Dairy Herds. J Clin Microbiol. 2011 Mar; 49(3):893-901.
  • Raizman, E. A., S. J. Wells, C. A. Munoz-Zanzi , and S. Tavornpanich. 2011. Estimated within-herd prevalence (WHP) of Mycobacterium avium subsp. paratuberculosis in a sample of Minnesota dairy herds using bacterial culture of pooled fecal samples. Can J Vet Res. 2011 April; 75(2):112-116.