Progress 10/01/10 to 09/30/15
Outputs
Target Audience:Common bean growers of Idaho and the US at large. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?One student graduated with a doctoral degree and one post-doc was trained. How have the results been disseminated to communities of interest?Through the publication of research articles, distribution of improved germplasm, and presentations in national and international conferences. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Resistance to two major diseases (bean common mosaic virus and bean rust) and soil zinc deficiency was incorporated into three new pinto bean cultivars. While doing that we broaden the genetic base of this most important market class by using multiple-parent inter-gene pool and inter-specific crosses. Furthermore, we demonstrated that for breeding for high levels of white mold resistance it was essential to use highly disease conducive environment in the greenhouse, diverse sources of resistant germplasm, multiple inoculations per plant with both less-aggressive and aggressive pathogen isolates of diverse origins/compatibility groups, multiple evaluations between 7 and 60 days post the first inoculation, and verification of the resistance response at harvest.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Singh, S.P., and Miklas, P.N. 2015. Breeding common bean for resistance to common blight: A review. Crop Sci. 55:971-984.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Viteri, D., Otto, K., Ter�n, H., Schwartz, H., and Singh, S.P. 2015. Use of four Sclerotinia sclerotiorum isolates of different aggressiveness with multiple inoculations and evaluations to select common beans with high levels of white mold resistance. Euphytica 204:457-472.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Viteri, D., and Singh, S.P. 2015. Inheritance of white mold resistance in an Andean common bean A 195 and its relationship with G122. Crop Sci. 55:44-49.
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Dr. Diego Viteri completed his Ph.D. degree in May 2014. He worked on breeding and genetics of common blight and white mold resistance in common bean. How have the results been disseminated to communities of interest? Through articles published in refereed journals. What do you plan to do during the next reporting period to accomplish the goals? Seed of five white mold resistant breeding lines will be multiplied and distributed upon request to public and private researchers. Also, two manuscripts will be prepared for registration in the Plant Registration Journal alog with seed deposited in the National Plant Germplasm facilities at Ft. Collins, Colorado.
Impacts
What was accomplished under these goals?
White Mold Resistance. Seed of one interspecific pinto bean breeding line, namely VCP 13 derived from a recurrent backcross of pinto 'UI 320*2/PI 439534 (Phaseolus coccineus, a member of the secondary gene pool), and one pinto (PRP 153) and three Andean (PRA 152, PRA 154, PRA 155) breeding lines with pyramided high levels of white mold (caused by Sclerotinia sclerotiorum) resistance from across Phaseolus species was multiplied in the field at Kimberly, Idaho in 2014. They also were simultaneously screened on the same plants against the pathogen isolates ARS12D, CO467, ND710, and NY133 in the greenhouse at Kimberly, Idaho. Furthermore, approval for the public release from the Idaho Foundation Seed Program Committee and the Director of Idaho and Colorado Agricultural Experiment Stations has been requested. Hopefully, these will be released for public use in December 2014, and registered in the Journal of Plant Registration in the spring of 2015. Their seed for research purposes should be available some time in 2015. Common Blight Resistance. An advanced tepary bean (P. acutifolius, a member of the tertiary gene pool of the common bean) derived common blight (caused by Xanthomonas campestris pv. phaseoli) resistant pinto PTRXa11.4 breeding line was developed. This breeding line has partial resistance in leaves and pods to less aggressive bacterial strain (e.g., ARX8AC), but confers a high level of resistance against aggressive (e.g., Xcp25) strain in leaves. We also helped develop, in collaboration with Dr. Tim Porch Andean breeding lines 08SH-840 and CXR 1, and in collaboration with Drs. Mari Carmen Asensio of Spain RCS 63 with high levels of pyramided common blight resistance. Seed of these breeding lines will be multiplied in 2015 for public release and registration in the Journal of Plant Registration.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Duncan, R.W., Gilbertson, R.L., Lema, M., and Singh, S.P. 2014. Inheritance of resistance to the widely distributed race 6 of Pseudomonas syringae pv. phaseolicola in common bean pinto US14HBR6. Can. J. Plant Sci. 94:923-928.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Sikora, E.J., et al. 2014. A coordinated effort to manage soybean rust in North America: A success story in soybean disease monitoring. Plant Dis. 98:865-875.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Singh, S.P., Schwartz, H.F., Teran, H., Viteri, D.M., and Otto, K. 2014. Pyramiding white mold resistance between and within common bean gene pools. Can. J. Plant Sci. 94:947-953.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Singh, S.P., Schwartz, H.F., Viteri, D.M., Teran, H., and Otto, K. 2014. Introgressing white mold resistance from Phaseolus coccineus PI 433246 into common pinto bean. Crop Sci. 54:1026-1032.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Teran, H., Jara, C., Mahuku, G., Beebe, S., and Singh, S. P. 2013. Simultaneous selection for resistance to five bacterial, fungal, and viral diseases in three Andean x Middle American inter-gene pool common bean populations. Euphytica 189:283-292.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Viteri, D.M., Cregan, P.B. Trapp, J., Miklas, P.N., and Singh, S.P. 2014. A new common bacterial blight resistance QTL in VAX 1 common bean and interaction of the new QTL, SAP6, and SU91 with bacterial strains. Crop Sci. 54:1598-1608.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Viteri, D.M., and Singh, S.P. 2014. Response of 21 common beans of diverse origins to two strains of the common bacterial blight pathogen, Xanthomonas campestris pv. phaseoli. Euphytica 200:379-388.
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Progress 01/01/13 to 09/30/13
Outputs
Target Audience: Common bean researchers from around the country and participants in the W2150 Western Regional Project. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Mr. Diego Viteri, a doctoral student, completed his research projects on white mold and common bacterial blight resistance. How have the results been disseminated to communities of interest? The research results were presented during the Biennial Meeting of the Bean Improvement Cooperative and the annual meeting of the W-2150 meeting held in Portland, OR in October. What do you plan to do during the next reporting period to accomplish the goals? Seed of two pinto and three Andean beans with high levels of white mold resistance will be multiplied, registration and germplasm release documents will be prepared, and research results published in refereed journals.
Impacts
What was accomplished under these goals?
White Mold Resistance. Only partial or low levels of resistance to white mold caused by Sclerotinia sclerotiorum are found in Common bean. A two-pronged strategy, namely (1) introgression of resistance from the Phaseolus species of the secondary gene pool, and (2) pyramiding of resistance from across Phaseolus species was undertaken in collaboration with researchers at Colorado State University in 2003. We are very pleased to report that both projects were successfully completed in 2013 despite the fact that we did not have any external funding for the past three years. One interspecific breeding line derived from P. coccineus, and three breeding lines with pyramided high levels of resistance from across Phaseolus species were developed in 2013. Their seed will be multiplied for registration and public release in 2014. The inheritance of white mold resistance in Othello (susceptible) x A 195 (resistant) and A 195 x G 122 (resistant) crosses against the less aggressive and aggressive isolates of S. sclerotiorum was completed in 2013. The F1 was resistant and two complementary dominant genes controlled resistance against each isolate in Othello x A 195. The F1 also was resistant and there was no segregation in the F2 in response to the less aggressive isolate, and a single dominant gene controlled resistance in response to the aggressive isolate in A 195 x G 122. Common Bacterial Blight Resistance. The identification of new common bacterial blight (caused by Xanthomonas campestris pv. phaseoli) resistance QTL (quantitative trait loci) in VAX 1 interspecific breeding line was completed in collaboration with researchers at USDA-ARS, Prosser, Washington and Beltsville, Maryland in 2013. A new tepary (P. acutifolius, a member of the tertiary gene pool of the common bean) bean derived resistance QTL located on the Pv11 linkage group was identified in VAX 1, and its presence verified in VAX 3 of which VAX 1 was a parent. The new resistance QTL confers partial resistance in leaves and pods to less aggressive strain, but confers a high level of resistance against aggressive strain in leaves. Also, it interacts positively with other known resistance QTL from the common and tepary beans. Western Regional Bean Trial (WRBT) and National Cooperative Dry Bean Nursery (CDBN). We evaluated the 2013 WRBT and CDBN in replicated trials in the field under high inputs at Kimberly for general adaptation, growth habit, days to maturity, 100-seed weight, and seed yield. Seed is being cleaned for measuring yield, seed weight, and post-harvest seed coat color darkening. Data analyses and reporting will be done subsequently. National Bean White Mold Nursery (BWMN). We screened in the greenhouse at Kimberly the 2012 BWMN comprising approximately 35 common bean genotypes from public and private researchers in North America, using the modified cut-stem inoculation method. Data from the replicated trial was processed and sent to Dr. Jim Steadman at University of Nebraska, Lincoln. Release of Halo Blight Resistant Pinto Bean Breeding Line US14HBR6. Sufficient seed of halo blight resistant pinto bean breeding line, US14HBR6, the first of its kind, was produced at Kimberly under high input. Necessary document was prepared for release in December 2012. US14HBR6 also was registered in the Journal of Plant Registration.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Schwartz, H.F. and Singh, S.P. 2013. Breeding common bean for resistance to white mold: A review. Crop Science 53:1-13. Doi: 10.2135/cropsci2013.02.0081.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Ter�n, H., Jara, C., Mahuku, G., Beebe, S. and Singh, S.P. 2013. Simultaneous selection for resistance to five bacterial, fungal, and viral diseases in three Andean x Middle American inter-gene pool common bean populations. Euphytica 189:283-292.
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Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: White Mold Resistance. Forty-two breeding lines with high levels of white mold resistance and seven susceptible and resistant controls were evaluated against an aggressive (ND) and less-aggressive (AR) isolates of S. sclerotiorum in the greenhouse at Kimberly. The 42 genotypes included interspecific breeding lines derived from Phaseolus coccineus and P. costaricensis, and breeding lines derived from crosses among white mold resistant large-seeded Andean germplasm and from multiple-parent crosses involving Phaseolus species of the primary and secondary gene pools. A randomized complete block design with three replicates was used. Each plant consisted of 6 plants, which were inoculated three times beginning at the fifth inter-node with 48 hours old culture of S. sclerotiorum and evaluated at 7, 14, 21, 28, and 35 days post the first inoculation. Three interspecific and four breeding lines with pyramided resistance were selected for further evaluations and release to public and private researchers. Parents, F1, and F2 of pinto Othello x A 195 and A 195 x G 122 were evaluated against the less aggressive and aggressive isolates of S. sclerotiorum in the greenhouse. Othello is highly susceptible to white mold, and A 195 and G 122 are highly resistant. Data is being compiled and analyzed. Common Bacterial Blight Resistance. We screened several dozen F6-derived F7 recombinant inbred lines from Othello x VAX 1 and Othello x VAX 3 crosses to identify new common bacterial blight resistance QTL derived from the tepary bean, and to determine the response of SU91 in the pod. Our results indicate that while SU91 linked QTL confers high levels of resistance in leaves, it has no effects on pods when inoculated with aggressive as well as less aggressive bacterial isolates. We have identified several recombinant inbred lines resistant to common bacterial blight that do not carry known markers. These should be of immense value for breeding common bean of all colored as well as various white market classes. The search for new tepary bean derived resistance QTL is being carried out in collaboration with researchers at Prosser, Washington, Beltsville, Maryland, and Fargo, North Dakota. Western Regional Bean Trial (WRBT) and National Cooperative Dry Bean Nursery (CDBN). We evaluated the 2012 WRBT with 24 dry bean cultivars and breeding lines and the 2012 CDBN with 20 genotypes. The WRBT and CDBN were evaluated in replicated trials in the field under high inputs at Kimberly for general adaptation, growth habit, days to maturity, 100-seed weight, and seed yield. Seed is being cleaned for measuring yield, seed weight, and post-harvest seed coat color darkening. Data analyses and reporting will be done subsequently. PARTICIPANTS: S.P. Singh, Diego Viteri and Carlos Centeno, University of Idaho, Kimberly R & E Center, Idaho. H.F. Schwartz, Colorado State University, Fort Collins, Colorado. P.N. Miklas, USDA-ARS, Prosser, Washington. TARGET AUDIENCES: Public and Private Researchers in the United States and dry bean producers nationwide. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
None yet.
Publications
- No publications reported this period
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Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: The major research activities during the year included (1) evaluation of the regional and national cooperative nurseries, and (2) breeding for white mold resistance. A brief account of these activities is reported here. The evaluation of the regional and national cooperative nurseries in 2011 included the Western Regional Bean Trial (WRBT) with 25 dry bean cultivars and breeding lines, national Cooperative Dry Bean Nursery (CDBN) with 32 genotypes, and National Bean White Mold Nursery (BWMN) comprising 13 dry and green bean breeding lines and cultivars. The WRBT and CDBN were evaluated in replicated trials in the field under high inputs at Kimberly for general adaptation, growth habit, days to maturity, 100-seed weight, and seed yield. Seed is being cleaned for measuring yield, seed weight, and post-harvest seed coat color darkening. The BWMN with three replicates was evaluated under severe disease pressure in the greenhouse at Kimberly. Dry bean genotype A 195 exhibited the highest level of resistance to white mold. For breeding for white mold resistance, approximately 145 breeding lines and families derived from over a dozen inter-gene pool and interspecific crosses along with five controls were screened in the greenhouse, using the ND710 pathogen isolate and multiple inoculations and evaluations. With increasing number of inoculations (from 1 to 3) and delaying evaluations (evaluated at 7, 14, 21, 28, and 35 days post inoculation) the disease severity index increased. Thus, our preliminary results indicate that for breeding for higher levels of white mold resistance it may be worth considering use of multiple inoculations and evaluations. PARTICIPANTS: Singh, S.P., Centeno, C., Viteri, D., Duncan, R.W., Gilbertson, R.L., Miklas, P.N., Teran, H., Kelly, J.D., Smith, J.R., Schwartz, H.F., Lema, M., and Hayes. R. TARGET AUDIENCES: Dry bean researchers, producers and other clienteles of Idaho, western U.S., North America and worldwide. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
None
Publications
- Duncan, R.W., Singh, S.P., and Gilbertson, R.L. 2011. Interaction of common bacterial blight bacteria with disease resistance quantitative trait loci in common bean. Phytopathology 101:425-435.
- Miklas, P.N., Singh, S.P., Teran, H., Kelly, J.D., and Smith, J.R. 2011. Registration of common bacterial blight resistant cranberry dry bean germplasm line USCR-CBB-20. J. Plant Reg. 5:98-102.
- Singh, S.P., and Schwartz, H.F. 2010. Breeding common bean for resistance to insect pests and nematodes: A Review Can. J. Plant Sci. 91: 239-250.
- Singh, S.P., Teran, H., Lema, M., and Hayes. R. 2011. Selection for dry bean yield on-station versus on-farm conventional and organic production systems. Crop Sci. 51: 621-630.
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