Source: UNIVERSITY OF FLORIDA submitted to NRP
DEVELOPMENT OF BIOSENSORS FOR MEASURING METABOLISM AND BIOPHYSICAL ANALYTE TRANSPORT IN AGRICULTURAL SYSTEMS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
0223613
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Sep 1, 2010
Project End Date
Sep 1, 2015
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
Agricultural and Biological Engineering
Non Technical Summary
Technologies are needed which are capable of measuring metabolism and analyte transport in agricultural and bioenvironmental life science applications. Traditional design of these tools is conducted by experts in the technology and/or physical science fields. Due to the complex nature of living systems, these tools often suffer from many technical problems when being used by researchers in the life sciences. Thus, the goal of this research is to design, build, and apply sensor and biosensor technologies driven by specific hypothesis driven research. Using this approach, the technology is designed to be a "living" part of the system, while relaying real time data quantifiying system performance.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
4041440100015%
4041440101015%
4041440202020%
4044010100015%
4044010101015%
4044010202020%
Knowledge Area
404 - Instrumentation and Control Systems;

Subject Of Investigation
1440 - Cole crops; 4010 - Bacteria;

Field Of Science
2020 - Engineering; 1010 - Nutrition and metabolism; 1000 - Biochemistry and biophysics;
Goals / Objectives
The overall goal of the proposed research is to develop and apply biosensor technologies for measuring metabolism and analyte transport in agricultural and bioenvironmental life science applications. To accomplish this, the following objectives are proposed: Objective 1 (years 1 and 2): Develop advanced microsensors for measuring metabolic oxygen transport and metabolism. Highly sensitive sensors are needed which can measure oxygen concentration in both the liquid and gas phase, provide real time monitoring, and be used in a non-invasive (non-destructive) modality. Thes can be achieved via development of lifetime-based luminescent fiber optic sensors, which has numerous advantages over commercially available sensors. Objective 2 (years 2 and 3): Glucose biosensor development for measuring cellular viability. Glucose is the central molecule in many biochemical pathways, and numerous approaches have been developed for fabricating biosensors designed to measure glucose concentration in/near cells and/or tissues. A novel enzyme-based glucose biosensor will be developed for measuring cell viability. The biosensor will be based on previous research by the PI, and will have high spatial resolution, excellent selectivity, rapid prototyping, low cost, and a simple operating scheme. Objective 3 (years 4 and 5): Develop a multi-analyte microsensor system for measuring ion concentration/flux. Many techniques for measuring electrophysiological ion transport lack sufficient temporal resolution, require pharmacological screening for quantifying ion transport in living cells, and/or have cytotoxicity issues. The advantages of the proposed design include: multiplexing capability, increased response time, improved sensitivity, and the ability to account for buffering artifacts. The ability to sense molecules related to metabolism and active biophysical tranrpot will improve our understanding of plant gravitropism, bioenergy processes, and many other applications by providing real time, dynamic analysis of living cells/organisms under physiological conditions. Results from this research will be used to enhance our understanding of the dynamics of analyte transport in engineered and natural plant/microbial systems (including design and modeling efforts). The proposed research aims to develop a platform of tools designed to support life science research, which will be expanded to include additional analytes and also support other interdisciplinary research within the life sciences.
Project Methods
Using simple recognition-transduction schemes, biosensors can be fabricated for detecting many different analytes related to life sciences research (Bakker and Qin, 2006; Porterfield, 2007; McLamore et al., 2010a). This proposal will focus on the development of biosensors for measuring oxygen, glucose, pH, and calcium. Oxygen microsensors will be fabricated based on previously published techniques using silica glass fiber optic cables (Porterfield et al., 2007; Chatni and Porterfield 2009; McLamore et al., 2010b). Tapered fiber optic cables will be decorated with a novel metal nanoparticle/fluorescent dye matrix and housed within a needle-type syringe. Glucose micro biosensors will be constructed from 2-5 μm tip diameter platinum/iridium wires containing immobilized carbon nanotubes, metal nanospheres, and enzymes (glucose oxidase) (McLamore et al., 2010c). Ion-selective microelectrodes for measuring H+ flux (Porterfield et al., 2009), and Ca2+ (Smith et al., 1999) will be constructed based on Porterfield et al. (2009) and Smith et al (1999). The PI has successfully and consistently constructed each of these microsensors for specialty applications, and will use this as a base of knowledge to develop implantable sensors for the proposed research. To demonstrate the applicability of the autonomous microsensor technologies, the sensors will be used for: (i) measuring oxygen/ion concentration near roots/leafs of radishes under conditions simulating growth on the lunar surface in low pressure plant growth chambers (Fig 2A), and (ii) measuring oxygen/glucose/ion transport at the surface of a microbial biofilm used for methane extraction within a hollow fiber silicone membrane based on McLamore et al (2007) and McLamore et al (2010d). Applications of sensors will be conducted under relatively harsh operating conditions, and will demonstrate the applicability of the sensing platform to support a wide range of life sciences applications.

Progress 09/01/10 to 09/01/15

Outputs
Target Audience:1. Extension agents 2. Pre-collegiate teachers 3. Pre-collegiate students 4. Collegiate students not majoring in engineering or science Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A total of 38 students participated in the accomplishments; including graduate and undergraduate students. How have the results been disseminated to communities of interest?Peer reviewed publications and presentations at scientific conferences What do you plan to do during the next reporting period to accomplish the goals?Although this CRIS project will be terminated, I will continue my program in collaboration with a multistate project (NC-1194) The planned activities are described below Research Goals Plans for continuation of current projects: I hired a new MS student in Jan 2016 to work on my NSF project I will continue to develop momentum using my current grants from NSF and NASA Publishing manuscripts (8 planned and 6 in review) Assisting students with writing dissertation/thesis Planned proposals and collaborations: I am continuing to strengthen my program in the area of food safety, and beginning to expand into food security by developing technologies to be used in humanitarian/refugee crises I have been working extensively in South America (primarily Colombia) developing biosensors for food safety with Universidad del Valle I completed a MOU with Universidad del Valle (pending review by UV) ties (Colombia, Ecuador, and Honduras) I applied for a teaching Fulbright program to strengthen my research in food safety and collaboration with UV I am drafting 7 manuscripts with colleagues from UV I will continue my research with Peking Univ. and Tsinghua Univ. in Beijing related to nutrition, food, and diabetes I am drafting 2 papers with colleagues from Beijing related to plant physiology and food/nutrition I was invited to be a keynote speaker at IFT (Chicago, 2016) I was invited to be a keynote speaker in Malaysia for the International Conference on Biotechnology Engineering, specifically focusing on humanitarian aid related to food/water safety and natural medicines Other research plans: I am talking with National Instruments (Planet NI program) to develop a global biosensor program related to humanitarian aid (NI funds equipment and training programs overseas) Extension Plans for continuation of existing programs: Continue 4H summer camp Plans for new programs: I will be developing biological engineering camp activities in Spanish I will write and submit an article to resource magazine In January, I have expanded the biosensors camp to pre-K and K-6 children in Gainesville Planned Extension outcomes/outputs/impacts: Build larger pool of group learning participants by expanding to K-6 children and K-6 teachers (not related to 4H) Other Extension plans: none Teaching Current courses Biosensors (split G/UG) Grant Writing (split G/UG) Senior Design 2 Proposals for new courses or changes/revisions to existing courses: I have spoken with Jim and Melanie about the idea of revamping the Intro class and converting the space behind my lab to a state of the art teaching lab as the "home" Graduate committees: I currently chair 1 PhD student and 1 MS student Plans for new students: None at this time Plans for graduations: None Publications: Planned: 8 total Taguchi, M., H. Hahn, Kiernan-Lewis, J., Byers, K., E.S. McLamore. A rapid point of care biosensor for measuring catecholamines in urine as an indicator of PTSD. Analyst, In review. Rong, Y., S. Chi, S.H. Ying, J.X. Zhang, N.O. Keyhani, E.S. McLamore. Nanometal-decorated electrodes for studying protein-protein interactions using impedance spectroscopy. Analyst, in review Rong, Y., Samyal, Byers, K. Ishika, E.S. McLamore. Insect derived chemosensory proteins for rapid monitoring of biomarkers of diabetic ketoacidosis in human saliva. Biosensors and Bioelectronics, In review Groszman, K., D.C. Vanegas, S.L. Burrs, C. Gomes, E.S. McLamore. A nanocellulose-bioflavanoid-nanoceria hybrid composite for rapid point of care biosensing. Analyst, In review Vanegas, D.C. J.C. Claussen, C. Gomes, E.S. McLamore. Emerging technologies for rapid monitoring of bacteria and bacterial biomarkers in food. Inter. J. Food Sci. In review Wang, J., D.C. Vanegas, E.S. McLamore, Y. Shen. A non-invasive microsensor for measuring insulin transport in pancreatic islets. J. Diabetes Sci. Tech. In review Taguchi, M., Kiernan-Lewis, J., N. Schwalb, C. Gomes, J.C. Claussen, E.S. McLamore. Synthesis of nanobrush polymers for biosensing using pulsed sonoelectrodeposition. Analyst. In review Burrs, S.L., M. Bhargava, J. Kiernan-Lewis, N. Schwalb, C. Gomes, J. Claussen, E.S. McLamore. A paper based graphene-nanocauliflower hybrid composite for point of care biosensing. Biosensors and Bioelectronics, In review In review: 6 total In Review: 6 total Taguchi, M., N. Schwalb, D.C. Vanegas, N. Garland, M. Tan, H. Yamaguchi, J.C. Claussen, E.S. McLamore. pulSED: Pulsed sonoelectrodeposition of nanometal for enhancing electrochemical biosensor performance. Analyst, In review Chaturvedi, P., D.C. Vanegas, J. Foster, B.A. Hauser, M.S. Sepulveda, E.S. McLamore. A graphene-nanoceria hybrid microsensor for physiological studies of nitric oxide flux. Analyst, In review Gillooly, J.F., J.P. Gomez, E. Mavrodiev, Y. Rong, E.S. McLamore. Structural Constraints on Oxygen Diffusion Explain Body Mass Scaling of Metabolic Rate. Proc. Nat. Acad. Sci. USA, In review Chi, S., Y. Rong, E.S. McLamore, S.H. Ying, J.X. Zhang, N.O. Keyhani. Interaction between TATA-binding protein (TBP) and multiprotein bridging factor-1 (MBF1) from the filamentous insect pathogenic fungus Beauveria bassiana. J. Biol. Chem., in review Yan, S., S. Dong, E.S. McLamore, T. Zhang, N. Wang, H. Yao, Y. Shen. Jasmonate as a long distance signal affected the auxin flux along the root tip in Arabidopsis thaliana upon herbivory, Plant Physiology and Biochemistry, In review Wang, N., E.S. McLamore, Y. Shen. Effect of drought stress on photosynthesis, water use efficiency and stable isotope composition in wild Davidia involucrata Baill seedling leaves, PLoS One, In review

Impacts
What was accomplished under these goals? The major accomplishments are primarily related to objective 3, although some accomplishments were related to objective 2 Sensors related to glucose measurement as an indicator of cellular activity were developed using various nanomaterial/biomaterial combinations. Some of these designs were applied in cancer research related to food/nutrition in collaboration with research labs at Purdue University (Teegarden, Donkin, Porterfield) Burrs, S.L., D.C. Vanegas, P. Hendershot, H. Greenslet, C. Gomes, E.S. McLamore (2015). A Comparitive Study of Graphene-Hydrogel Hybrid Nanocomposites for Biosensing. Analyst, 140: 1466-1476 Zheng, W., F. Tayyari, N. Gowda, D. Raftery, E.S. McLamore, D.M. Porterfield, B. Bequette, S. Donkin, D. Teegarden (2015). Altered glucose metabolism in Harvey-ras transformed MCF10A cells, Molecular Carcinogenesis, 54(2):111-120. Biosensor technologies were developed for monitoring plant cell signaling and used to study the electrical signals involved with stress response related to herbivory and heat stress/drought. Deng, W.H., B. Chen, W. Deng; Y.Q. Zhang; S.L. Yan; H.B. Gao; J.Q. Zhang; E.S. McLamore; D.C. Vanegas; Y.B. Shen; B. Wu (2016) Extracts of Artemisia ordosica can influnce the growth of associated plants by regulating the absorption of NH4+ and K+. Allelopathy Journal, In press Yan, S., T. Zhang, S. Dong, E.S. McLamore, N. Wang, X. Shan, Y. Wan, Y. Shen. MeJA affected root growth by modulation of transmembrane auxin flux in transition zone (2015). Journal of Plant Growth Regulation, In press; DOI 10.1007/s00344-015-9530-9 Yan, S., E.S. McLamore, D. Shanshan, H. Gao, M. Taguchi, N. Wang, T. Zhang, X. Su, Y. Shen (2015) The role of plasma membrane H+-ATPase in jasmonate-induced ion fluxes and stomatal closure in Arabidopsis thaliana. The Plant Journal. 83(4): 638-649. Vanegas, D.C., A. Cannon, G. Clark, S. Roux, E.S. McLamore (2015). A self referencing microbiosensor for measuring ATP flux. Biosensors and Bioelectronics. 74:37-44 Wright, S. B. Welt, P. Pratap, E.S. McLamore, A. Wilkie (2015). A Review on the Current State of Autotrophic Phytoplankton Growth and Lipid Synthesis Modeling. Trans. ASABE, 7(4): 143-168 Marvasi, M., D.C. Vanegas,. P. Chaturvedi, E.S. McLamore, M. Tepliski (2015). Salmonella enterica biofilm-mediated dispersal by nitric oxide donors in association with cellulose nanocrystal hydrogels. AMB Express, 5:28

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: 1) Deng, W.H., B. Chen, W. Deng; Y.Q. Zhang; S.L. Yan; H.B. Gao; J.Q. Zhang; E.S. McLamore; D.C. Vanegas; Y.B. Shen; B. Wu (2015) Extracts of Artemisia ordosica can influnce the growth of associated plants by regulating the absorption of NH4+ and K+. Allelopathy Journal, In press 2) Yan, S., T. Zhang, S. Dong, E.S. McLamore, N. Wang, X. Shan, Y. Wan, Y. Shen. MeJA affected root growth by modulation of transmembrane auxin flux in transition zone (2015). Journal of Plant Growth Regulation, In press; DOI 10.1007/s00344-015-9530-9 3) Yan, S., E.S. McLamore, D. Shanshan, H. Gao, M. Taguchi, N. Wang, T. Zhang, X. Su, Y. Shen (2015) The role of plasma membrane H+-ATPase in jasmonate-induced ion fluxes and stomatal closure in Arabidopsis thaliana. The Plant Journal. 83(4): 638-649. 4) Vanegas, D.C., A. Cannon, G. Clark, S. Roux, E.S. McLamore (2015). A self referencing microbiosensor for measuring ATP flux. Biosensors and Bioelectronics. 74:37-44 5) Wright, S. B. Welt, P. Pratap, E.S. McLamore, A. Wilkie (2015). A Review on the Current State of Autotrophic Phytoplankton Growth and Lipid Synthesis Modeling. Trans. ASABE, 7(4): 143-168 6) Marvasi, M., D.C. Vanegas,. P. Chaturvedi, E.S. McLamore, M. Tepliski (2015). Salmonella enterica biofilm-mediated dispersal by nitric oxide donors in association with cellulose nanocrystal hydrogels. AMB Express, 5:28 7) Burrs, S.L., D.C. Vanegas, P. Hendershot, H. Greenslet, C. Gomes, E.S. McLamore (2015). A Comparitive Study of Graphene-Hydrogel Hybrid Nanocomposites for Biosensing. Analyst, 140: 1466-1476 8) Zheng, W., F. Tayyari, N. Gowda, D. Raftery, E.S. McLamore, D.M. Porterfield, B. Bequette, S. Donkin, D. Teegarden (2015). Altered glucose metabolism in Harvey-ras transformed MCF10A cells, Molecular Carcinogenesis, 54(2):111-120.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: 1) Vanegas, D.C.g, Y. Rongg, N. Schwalbu, K.D. Hills, C. Gomes ; E.S. McLamore (2015) Rapid detection of Listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures. Proceedings of SPIE-International Society of Optical Engineering. Baltimore, MD. Paper No. 9487-08 2) Vanegas, D.C.g, Y. Rongg, N. Schwalbu, K.D. Hills, C. Gomes ; E.S. McLamore (2015) Rapid detection of Listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures. International ASABE Meeting, New Orleans, LA 3) 2. Vanegas, D.C.g, Y. Rongg, N. Schwalbu, K.D. Hills, C. Gomes ; E.S. McLamore (2015) Rapid detection of Listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures. International Food Technology Conference, Chicago, IL 4) Rong, Y.g, N.O. Keyhani, A. Ortiz, E.S. McLamore (2015) Chemosensory proteins based electrochemical biosensor for diagnosis of diabetic ketoacidosis by detecting acetone. 4th International Conference on Bio-Sensing Technology, Lisbon, Portugal. 5) Vanegas, D.C.g, Y. Rongg, N. Schwalbu, K.D. Hills, C. Gomes, J.C. Claussen, E. S. McLamore (2015) Rapid detection of Listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures. SPIE-International Society of Optical Engineering. Baltimore, MD. 6) Rong, Y.g, N.O. Keyhani, A. Ortiz, E.S. McLamore (2015) Chemosensory proteins based electrochemical biosensor for diagnosis of diabetic ketoacidosis by detecting acetone. ABEGSO Graduate Student Poster Competition, Gainesville, FL
  • Type: Theses/Dissertations Status: Published Year Published: 2015 Citation: 1) Vanegas, D. (2015) NANOMATERIAL MEDIATED BIOSENSORS FOR AGRICULTURAL AND FOOD SAFETY APPLICATIONS, PhD Dissertation, University of Florida 2) Burrs, S.L. (2015) DEVELOPMENT OF PLATINUM GRAPHENE NANOCOMPOSITE BIOSENSORS FOR AMPEROMETRIC SENSING, PhD Dissertation, University of Florida 3) Taguchi, M. (2015) DEVELOPMENT OF PULSED SONOELECTRODEPOSITION TECHNIQUES FOR SENSOR AND BIOSENSOR APPLICATIONS, PhD Dissertation, University of Florida
  • Type: Book Chapters Status: Accepted Year Published: 2015 Citation: 1) McLamore, E.S., M. Convertino, I. Oksoy, M. Taguchi, D.C. Vanegas, C. Gomes, J.C. Claussen. Book Chapter: Biomimetic Fractal Nanometals as a Transducer Layer in Electrochemical Biosensing. In: Semiconductor Device-Based Sensors for Gas, Chemical, and Biomedical Applications. Ed by Fan Ren and Stephen J Pearton, CRC Press; ISBN: 1439813876


Progress 10/01/13 to 09/30/14

Outputs
Target Audience:1. Extension agents 2. Pre-collegiate teachers 3. Pre-collegiate students 4. Collegiate students not majoring in engineering or science Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A total of 29 students participated in the accomplishments; including graduate and undergraduate students. How have the results been disseminated to communities of interest?Peer reviewed publications and presentations at scientific conferences What do you plan to do during the next reporting period to accomplish the goals?Research Goals I will be graduating 3 of my 4 PhD students in the summer. Publishing manuscripts and assisting students with writing the dissertation will be the main focus of my time for the first half of 2015 (currently preparing 5 manuscripts with my students) Planned proposals and collaborations: I have shifted a large portion of my focus to food safety. I will continue to strengthen these relationships through collaborators around the U.S. I am planning international work in South America involving biosensors for food safety with Universities (Colombia, Ecuador, and Honduras) I am starting the process to apply for sabbatical trips to South America to strengthen my research in food safety (Fulbright, UF Prof. Dev., and Colciencias) I will continue my research with Peking Univ. and Tsinghua Univ. in Beijing related to nutrition, food, and diabetes I am drafting 6 papers with colleagues from Beijing related to plant physiology and food/nutrition Other research plans: Extension Plans for continuation of existing programs: Continue 4H summer camp Plans for new programs: I will be developing biological engineering camp activities in Spanish I will write and submit an article to resource magazine Planned Extension outcomes/outputs/impacts: none Other Extension plans: none Teaching Current courses Biosensors (split G/UG) Grant Writing (split G/UG) Senior Design 2 Proposals for new courses or changes/revisions to existing courses: none Graduate committees: I currently chair 4 PhD students Plans for new students: None at this time Plans for graduations: Diana Vanegas (Summer), Stephanie Burrs (Summer), Masashige Taguchi (Fall) Publications: Planned: Preparing 16 manuscripts for submission in 2015 In review: 5 currently in review

Impacts
What was accomplished under these goals? The accomplishments are primarily related to objective 2, although progress in objective one and three was also made. In regards to objective one, a sensor system was developed for monitoring soil oxygen levels related to biological activity in collaboration with the EPA (J. Garland) and researchers in IFAS (McLamore, Mackowiak, Ullman) McLamore, E.S., J.L. Garland, C. Mackowiak, A. Desaunay, N. Garlandu, P. Chaturvedig, M. Taguchig, K. Dreadeng, J. Catechis, J.L. Ullman (2014), Development and validation of an open source O2-sensitive gel for physiological profiling of microbial communities. J. Micro. Meth., 96: 62-67. Sensors related to glucose measurement as an indicator of cellular activity were developed using various nanomaterial/biomaterial combinations. Some of these designs were applied in cancer research related to food/nutrition in collaboration with research labs at Purdue University (Teegarden, Donkin, Porterfield) Chaturvedi, P.g, J. Foster, Hauser B., E. Karplus, E.S. McLamore (2014). A Multiplexing Fiber Optic Microsensor System for Monitoring Oxygen in Biological Samples. Sens. Actuat. B: Chemical, 196: 71-79. Zheng, W., F. Tayyari, N. Gowda, D. Raftery, E.S. McLamore, D.M. Porterfield, B. Bequette, S. Donkin, D. Teegarden (2014). Altered glucose metabolism in Harvey-ras transformed MCF10A cells, Molecular Carcinogenesis, 54(2): 111-120, Zhang, M.g, B. Gao, D.C. Vanegasg, E.S. McLamore, J. Fang, L. Liu, L. Wu, H. Chen, M. Inyangg, Y. Yao, Y. Zhou, Y. Sun (2014). Simple approach for large-scale production of reduced graphene oxide films. Chemical Engineering Journal, 243: 340-346. Zhang, W., E.S. McLamore, D.M. Porterfield, M.K. Banks (2014) Glutathione gated potassium efflux as a mechanism for active biofilm detachment. Water Environment Research. 86(5): 462-469. Vanegas, D.C.g, M. Taguchi g, P. Chaturvedi g, S.L. Burrs g, M. Tang, H. Yamaguchi, E.S. McLamore (2014). A comparative study of carbon-platinum hybrid nanostructure architecture for amperometric biosensing. Analyst, 39: 660-667. Chaturvedi, P.g, D.C. Vanegasg, S.L. Burrsg, M. Taguchig, P. Sharma, E.S. McLamore (2014). A nanoceria-platinum-graphene nanocomposite for electrochemical biosensors. Biosensors & Bioelectronics, 58: 179-185. Biosensor technologies were developed for monitoring ecotoxicity based on the physiological behavior of an aquatic organism. These multi-faceted response mechanisms were applied in lake water in collaboration with researchers at Purdue University (Sepulveda) Stensberg M.C.g, Zeitchek M.A., Inn K., McLamore E.S., Porterfield D.M., Sepulveda M.S. (2014) Comparative study of non-invasive methods for assessing Daphnia magna embryo toxicity. Environ Sci Pollut Res Int. (18): 10803-10814. Stensberg, M.C.g, R. Madangopal, G. Yaleg, Q. Wei, H. Ochoa-Acuna, A. Wei, E.S. McLamore, J.L. Rickus, D.M. Porterfield, M.S. Sepulveda (2014). Silver nanoparticle-specific mitotoxicity in Daphnia magna. Nanotoxicology, 8(8): 833-842.

Publications

  • Type: Book Chapters Status: Published Year Published: 2014 Citation: 1) Claussen, J.C., Campuzano, Pedrero, D.C. Vanegasg, E.S. McLamore, Kuralay, Daniele, A.W.A. Salim (2014) Chapter 11: Hybrid Metallic Nanoparticles: Enhanced Bioanalysis and Biosensing via Carbon Nanotubes, Grapene, and Organic Conjugation, In: Nanobiosensing and Nanobioanalyses (ed: M.C. Vestergaard, K. Kerman, I.M. Hsing, E. Tamiya. Springer Verlag
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: 1) Zheng, W., F. Tayyari, N. Gowda, D. Raftery, E.S. McLamore, D.M. Porterfield, B. Bequette, S. Donkin, D. Teegarden (2014). Altered glucose metabolism in Harvey-ras transformed MCF10A cells, Molecular Carcinogenesis, 54(2): 111-120, 2) Stensberg M.C.g, Zeitchek M.A., Inn K., McLamore E.S., Porterfield D.M., Sepulveda M.S. (2014) Comparative study of non-invasive methods for assessing Daphnia magna embryo toxicity. Environ Sci Pollut Res Int. (18): 10803-10814. 3) Zhang, M.g, B. Gao, D.C. Vanegasg, E.S. McLamore, J. Fang, L. Liu, L. Wu, H. Chen, M. Inyangg, Y. Yao, Y. Zhou, Y. Sun (2014). Simple approach for large-scale production of reduced graphene oxide films. Chemical Engineering Journal, 243: 340-346. 4) Stensberg, M.C.g, R. Madangopal, G. Yaleg, Q. Wei, H. Ochoa-Acuna, A. Wei, E.S. McLamore, J.L. Rickus, D.M. Porterfield, M.S. Sepulveda (2014). Silver nanoparticle-specific mitotoxicity in Daphnia magna. Nanotoxicology, 8(8): 833-842. 5) Vanegas, D.C.g, M. Taguchi g, P. Chaturvedi g, S.L. Burrs g, M. Tang, H. Yamaguchi, E.S. McLamore (2014). A comparative study of carbon-platinum hybrid nanostructure architecture for amperometric biosensing. Analyst, 39: 660-667. 6) Chaturvedi, P.g, D.C. Vanegasg, S.L. Burrsg, M. Taguchig, P. Sharma, E.S. McLamore (2014). A nanoceria-platinum-graphene nanocomposite for electrochemical biosensors. Biosensors & Bioelectronics, 58: 179-185. 7) Chaturvedi, P.g, J. Foster, Hauser B., E. Karplus, E.S. McLamore (2014). A Multiplexing Fiber Optic Microsensor System for Monitoring Oxygen in Biological Samples. Sens. Actuat. B: Chemical, 196: 71-79. 8) Zhang, W., E.S. McLamore, D.M. Porterfield, M.K. Banks (2014) Glutathione gated potassium efflux as a mechanism for active biofilm detachment. Water Environment Research. 86(5): 462-469. 9) McLamore, E.S., J.L. Garland, C. Mackowiak, A. Desaunay, N. Garlandu, P. Chaturvedig, M. Taguchig, K. Dreadeng, J. Catechis, J.L. Ullman (2014), Development and validation of an open source O2-sensitive gel for physiological profiling of microbial communities. J. Micro. Meth., 96: 62-67.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: 1) Vanegas, D.C.g, E.S. McLamore (2014). Real-time monitoring of food threats. Proceedings of SPIE-International Society of Optical Engineering. Baltimore, MD. Paper 9107-31. 2) Taguchi, M.g, N. Garlandu, N. Schwalbu, D.C. Vanegasg, S.L. Burrsg, E.S. McLamore (2014). A Pulsed Sonoelectrodeposition Platform for Nanomaterial-Mediated Biosensor Development. Proceedings of SPIE-International Society of Optical Engineering. Baltimore, MD. Paper 9107-29 3) Vanegas, D.C.g, G. Clark, S. Roux, E.S. McLamore (2014). A micro-biosensor for real-time monitoring of extracellular ATP. 19th Annual IBE Conference, Lexington, KY. 4) Burrs, S.L.g, D.C. Vanegasg, E.S. McLamore (2014). Development of Nanomaterial Mediated Biochips for Biofuel Research. 19th Annual IBE Conference, Lexington, KY. 5) Taguchi, M.g., N. Schwalbu, N. Garlandu, E.S. McLamore (2014). Development of a sonoelectrodeposition platform for nanometal deposition. 19th Annual IBE Conference, Lexington, KY. 6) Vanegas, D.C.g, C. Gomes, E.S. McLamore (2014) Real time monitoring of food threats. SPIE-International Society of Optical Engineering. Baltimore, MD. 7) Taguchi, M.g, N. Garlandu, N. Schwalbu, D.C. Vanegasg, S.L. Burrsg, E.S. McLamore (2014) A Pulsed Sonoelectrodeposition Platform for Nanomaterial-Mediated Biosensor Development 8) Cannon, A.E., D.C. Vanegas, G. Clark, E.S. McLamore, S.J. Roux (2014) A Gravity-Directed Gradient of Extracellular Nucleotides Directs Polarization in Ceratopteris Spores. American Society of Gravitation Space Research. New Orleans, LA.
  • Type: Theses/Dissertations Status: Published Year Published: 2014 Citation: 1) Chaturvedi, P (2014) DEVELOPMENT OF SENSORS FOR MONITORING OXYGEN AND FREE RADICALS IN PLANT PHYSIOLOGY, PhD Dissertation, University of Florida


Progress 10/01/12 to 09/30/13

Outputs
Target Audience: Biological engineering research labs Biological engineering teachers (collegiate) K-12 FL youth who attended the summer biosensors camp Precollegiate educators and students (website) Changes/Problems: Our group has initiated new projects that explore the fundamental concepts in more depth. Specifically, we are using common equipment such as DLP projectors to recreate nanostructures in my lab, rather than fabricating the structures in a controlled clean room. This is the first step towards creating user-friendly biosensors (and related electronics) that can be built anywhere in the world (on the farm, in a high school classroom, etc) What opportunities for training and professional development has the project provided? The principles from research activities were integrated into formal teaching activities in the following courses at UF: -ABE 4033: Fundamentals and Applications of Biosensors (3 undergrad students) -ABE 5038: Advanced Biosensors (6 PhD students) -ABE 4043C: Capstone Senior Design II (31 undergraduate students) Additionally, concepts were integrated into the following informal STEM activities -A biosensors summer camp (40 precollegiate FL youth and 4 high school teachers) -An Art/Science summer camp directed by E.S. McLamore (22 UF students and 13 community members) How have the results been disseminated to communities of interest? For formal teaching, results were disseminated by discussing the published manuscripts during classroom group work. For informal activities, participants constructed biosensors using household items based on the fundamental research concepts What do you plan to do during the next reporting period to accomplish the goals? New nanomaterials and biomaterials are being developed to expand the library of biosensors that can be used in agricltural sciences. Additionally, new hardware is being developed so that others can recreate the technology outside of the University where specialty equipment is available.

Impacts
What was accomplished under these goals? Objective 2: Two peer reviewed manuscripts were published describing glucose biosensor technology (Zheng et al., 2013; Shi et al., 2013) A book chapter was also submitted describing the technology (Claussen et al., in review)

Publications

  • Type: Book Chapters Status: Published Year Published: 2013 Citation: McLamore, E.S., and D.M. Porterfield (2013) Chapter 43: Non-invasive tools for measuring metabolism and biophysical analyte transport at the root-rhizosphere interface: Self referencing electrochemical and optical sensors, In: Plant Roots: The Hidden Half, 4th Edition (ed: A. Eshel), Marcel Dekker, Inc.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Xin, X., Y. Wan, W. Wang, G. Yin, E.S. McLamore, X. Lu (2013) A real-time, non-invasive, micro-optrode technique for detecting seed viability by using oxygen influx. Nature: Scientific Reports, 3(3057): 1-6
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Zhang, W., E.S. McLamore, N.T. Garland, J. L.C. Leon, M. Katherine Banks (2013) A Simple Method for Quantifying Biomass Cell and Polymer Distribution in Detached Biofilms, J. Microbio Methods, 94(3):367-374.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Chaturvedi, P., M. Taguchi, S.L. Burrs, B. Hauser, J. Claussen, E.S. McLamore (2013). Emerging technologies for non-invasive quantification of physiological oxygen transport, Planta, 238(3): 599-614
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Zheng, W., F. Tayyari, G.A.N. Gowda, D. Raftery, E.S. McLamore, J. Shi, D.M. Porterfield, B. Bequette, S. Donkin, D. Teegarden (2013). 1,25 Dihydroxyvitamin D Regulation of Glucose Metabolism in Harvey-ras Transformed MCF10A Human Breast Epithelial Cells. Journal of Steroid Chemistry and Molecular Biology, 138: 81-89
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Shi, J., E.S. McLamore, D.M. Porterfield (2013) Nanomaterial Based Self-referencing Microbiosensors for Cell and Tissue Physiology Research, Biosensors and Bioelectronics, 40(1): 127-134.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Huang, Z., E.S. McLamore, W. Zhang, H.S. Chang, S.T. Wereley, J.V.C. Leon, M.K. Banks (2013). Shear induced passive biofilm detachment from hollow fiber membranes. Biotechnology and Bioengineering, 110(2): 525-534.
  • Type: Book Chapters Status: Under Review Year Published: 2014 Citation: Claussen, J.C., Campuzano, Pedrero, D.C. Vanegas, E.S. McLamore, Kuralay, Daniele, A.W.A. Salim (2014) Chapter 11: Hybrid Metallic Nanoparticles: Enhanced Bioanalysis and Biosensing via Carbon Nanotubes, Grapene, and Organic Conjugation, In: Nanobiosensing and Nanobioanalyses (ed: M.C. Vestergaard, K. Kerman, I.M. Hsing, E. Tamiya). Springer Verlag


Progress 10/01/11 to 09/30/12

Outputs
OUTPUTS: Provided online (interactive) guest lectures for graduate courses in plant physiology to Beijing Forestry University and Capital Normal University (Beijing, China); total of graduate 129 students Assessed 42 participants in the UF Summer Biosensors Camp 36 primary school students 6 4H Extension Agents and high school teachers Activities Directed and garnered funding for a K-12 summer camp in collaboration with local 4H Extension agents Attended eight scientific national conferences related to biosensors and life sciences research Conducted 12 experiments related to development of biosensors for educational purposes using common household items Conducted 18 experimental studies that enhance training and development of four PhD graduate students at UF. Tested a soil nutrient assay using soil samples from a non-profit environmental company in Vermont. Mentor for one graduate teaching assistant Mentor for 4 PhD students attain career goals Mentor for 3 undergraduate scholars Mentor for one summer intern high school student Taught 3 classes to undergraduate and graduates (at the University of Florida (main campus); total of 17 students Services Provided a total of 97 consultations in the form of field, office, telephone, and email Products Conducted experiments to develop a soil nitrogen assay that will be used to improve water quality monitoring in agriculture. Developed a new methanol biosensor for biofuel monitoring Developed a new calcium sensor for monitoring plant physiology Developed a xanthine, hypoxanthine, and ATP biosensors for monitoring meat and seafood quality Developed new oxygen and nitric oxide sensors for studying stress in developing plants and seeds PARTICIPANTS: E.S. McLamore was the principal investigator of the project, and was responsible for all project management, lead sensor development efforts, mentored students regarding sensor development, and trained/recruited graduate students for future research. P. Chutaverdi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Mrs. Chutaverdi also started the inaugural UF student chapter of the Institute of Biological Engineering and helped recruit students for future sensor development work. In fall 2012, Mrs. Chaturvedi successfully completed her PhD qualifying examination and is expected to graduate in 2013. S. Burrs is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. M. Taguchi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. D. Vanegas is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. K. Ward is an ABE undergraduate student who took sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. P. Pullammanappallil is an Associate professor in ABE collaborating on the start of an bioenergy project with Dr. Garland on the development and construction of membrane aerated bioreactors for methane extraction. S. Wilson is an extension specialist at the Walton County Extension Office who assisted in the development of the inaugural Biosensors Summer Camp in collaboration with the 4H summer camps. TARGET AUDIENCES: The target audience is graduate and undergraduate students, collaborators, co-investigators, and interested/concerned citizens. S. Wilson was responsible for organizing a target audience of K-12 students for the Biosensors camp, which will be lead by PI McLamore. This group was made up of a diverse mix of K-12 students from a variety of social, economic, or educational backgrounds. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Biosensors developed by our research group were used in 14 hypothesis driven research projects in the environmental and agricultural research area. My research group hosted a K-12 summer camp at UF for teaching youth and primary school educators how to build, design and use biosensors and sensors. Participants E.S. McLamore was the principal investigator of the project, and was responsible for all project management, lead sensor development efforts, mentored students regarding sensor development, and trained/recruited graduate students for future research. P. Chutaverdi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Mrs. Chutaverdi also started the inaugural UF student chapter of the Institute of Biological Engineering and helped recruit students for future sensor development work. In fall 2012, Mrs. Chaturvedi successfully completed her PhD qualifying examination and is expected to graduate in 2013. S. Burrs is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. M. Taguchi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. D. Vanegas is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. K. Ward is an ABE undergraduate student who took sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. P. Pullammanappallil is an Associate professor in ABE collaborating on the start of an bioenergy project with Dr. Garland on the development and construction of membrane aerated bioreactors for methane extraction. S. Wilson is an extension specialist at the Walton County Extension Office who assisted in the development of the inaugural Biosensors Summer Camp in collaboration with the 4H summer camps. Target Audience The target audience is graduate and undergraduate students, collaborators, co-investigators, and interested/concerned citizens. S. Wilson was responsible for organizing a target audience of K-12 students for the Biosensors camp, which will be lead by PI McLamore. This group was made up of a diverse mix of K-12 students from a variety of social, economic, or educational backgrounds. Awards and Honors Students in my group won multiple competitive awards Featured Spotlight Article in Biotechnology and Bioengineering NSF Research Experience for Undergraduates: Best Presentation Runner up (S. Frey, 2012) UF Assistant Professor Excellence Award (one annual award per college), 2012 IBE Annual Conference, Undergrad. Student Poster Competition Grand Prize (M. Porterfield, 2012) UF Marine Biology Symposium, Best Graduate Student Poster Award Runner Up (M. Taguchi, 2012)

Publications

  • Scholtz, R.V. III, E.S. McLamore, W. Porter (2012). A Systems View Towards More Sustainable Irrigation Design, Irrigation & Drainage Systems Engineering, 1(1): 1-2.
  • Sun, X., E.S. McLamore, D.M. Porterfield, and O. Akkus (2012). Bone matrix as a mechanochemical signal transducer--Mechanical Stretch Induced Calcium Efflux from Bone Matrix Stimulates Osteoblasts, Bone In Press.
  • Sun, X., E.S. McLamore, D.M. Porterfield, and O. Akkus (2012). Mechanical Stretch Induced Calcium Efflux from Bone Matrix Stimulates Osteoblasts. Proceedings of the European Society of Biophysics Annual Conference.
  • Ye, B., W. Wang, S.L. Burrs, E.S. McLamore, Y. Xu (2012). Real Time Detection of IAA Fluxes Near Root Surfaces of Cotton, Maize, Rice and Wheat. Auxin 2012, Waikoloa Beach, HI.
  • Zhang, W., E.S. McLamore, R. Wu, M. Stensberg, D.M. Porterfield, M.K. Banks (2012) An Active Biofilm Detachment Mechanism in Pseudomonas aeruginosa: Glutathione-gated Potassium Efflux. 112th General Meeting of the American Society of Microbiology, San Francisco, CA.
  • Ihara, I., J. Schueller, E.S. McLamore, E. Nakano, K. Toyoda, K. Umetsu, H. Yamaguchi (2012). Effect of surface roughness on cleanability of milk fouling deposits on inner stainless steel tubing. International Conference of Agricultural Engineering. Valencia, Spain.
  • Justinvil, G., S.L. Burrs, D. Vanegas, E.S. McLamore (2012). A Nanomaterial-Mediated Biosensor for Measuring Sarcosine in Human Urine. McNair Undergraduate Research Symposium.
  • Shupler, M., K. Ward, Y. Zhang, E.S. McLamore (2012). Effects of Laundry-Derived Silver Nanoparticles on Microbes in a Hollow-Fiber Membrane Bioreactor Treating Household Graywater. University of Florida Journal of Undergraduate Research. University of Florida Honors Undergraduate
  • McLamore, E.S., P. Pullammanappallil, S.L. Burrs, W. Ji, J. Garland (2012) Biogas production from a hollow fiber membrane bioreactor. UF Innovation Grant Final Report
  • McLamore, E.S. (2012). Self-Referencing Microsensors for Studying Plant Physiology. Beijing Forestry University; College of Forestry, Beijing, China. McLamore, E.S. (2012). Non-Invasive Microsensor Technique (NMT): Theory, Methods, and Recent Advancements. Beijing Forestry University; College of Forestry, Beijing, China.
  • McLamore, E.S. (2012). Sensors and Biosensors: Tools for Quantifying Physiology. University of Georgia, College of Veterinary Medicine and UGA STEM Academy, Athens, GA.
  • Justinvil, G., S.L. Burrs, D. Vanegas, E.S. McLamore (2012). A Nanomaterial-Mediated Biosensor for Measuring Sarcosine in Human Urine. McNair Undergraduate Research Symposium. Gainesville, FL
  • Ward, K., M. Shupler, Y. Zhang, S.L. Burrs, D. Jaroch, J.L. Rickus, E.S. McLamore (2012) Effects of Silver Nanoparticles on a Graywater Hollow Fiber Membrane Bioreactor. University of Florida Microbiology Symposium. Gainesville, FL.
  • Burrs, S.L., W. Ji, P. Chaturvedi, M. Taguchi, E.S. McLamore (2012) Hollow Fiber Membrane Anaerobic Bioreactors for Methanogenic Biofilm Development. 17th Annual Institute of Biological Engineering Conference, Indianapolis, IN.
  • McLamore, E.S. (2012). Nanomaterial-mediated biosensors for physiological sensing. University of Florida/Florida State University Joint Chemosensory Symposium, Gainesville, FL.
  • Ward, K., M. Shupler, Y. Zhang, D. Jaroch, J.L. Rickus, E.S. McLamore (2012). Decentralized Graywater Recovery Using Bioreactors: Effects of Laundry-Derived Nanoparticles. National Meeting of the American Water Resources Association, Jacksonville, FL.
  • Justinvil, G., D. Vanegas, S.L. Burrs, K. Ward, P. Chaturvedi, M. Taguchi, T.H. Boyer, E.S. McLamore (2012). Nanomaterial-mediated enzymatic biosensors for monitoring urine chemistry. National Meeting of the American Water Resources Association, Jacksonville, FL.
  • Chaturvedi P., Hauser B.A., Allen L.H., Boote K.J., Karplus E., McLamore E.S. (2012). A Multiplexing Fiber Optic Microsensor System for Monitoring Oxygen Concentration in Plants during Simulated Climate Change. 17th Annual Institute of Biological Engineering Conference, Indianapolis, IN.
  • McLamore, E.S. (2012) Self referencing sensors: Non-invasive tools for transport at the single cell to tissue level. 17th Annual Institute of Biological Engineering Conference, Indianapolis, IN.
  • Porterfield, D.M. and E.S. McLamore (2012). Self-referencing microbiosensors for cell and tissue physiology research. 22nd Anniversary World Congress on Biosensors. Cancun, Mexico.
  • Stensberg, M., M. Zeitchek, E.S. McLamore, M.S. Sepulveda, D.M. Porterfield (2012) Oxygen consumption in Daphnia magna as a bioindicator for early detection of environmental contaminants . 17th Annual Institute of Biological Engineering Conference, Indianapolis, IN.
  • Zhang, W., E.S. McLamore, M. Stensberg, R. Wu, D.M. Porterfield, M.K. Banks (2012). Passive and Active Detachment of Pseudomonas aeruginosa Biofilm at an Early Developing Stage. 17th Annual Institute of Biological Engineering Conference, Indianapolis, IN. McLamore, E.S. (2012). Non-invasive tools for measuring metabolism and biophysical analyte transport: self-referencing physiological sensing. UF Marine Biology Symposium. St. Augustine, FL.
  • Groszman, K., S.L.Burrs, D. Vanegas, A. Texeira, E.S. McLamore (2012). Methods for Teaching Biosensors Using Anthocyanin from Cabbage Juice. Indian River High School, Student Science Training Program.
  • Justinvil, G., S.L. Burrs, D. Vanegas, M. Taguchi, P. Chaturvedi, E.S. McLamore (2012). A Nanomaterial-Mediated Biosensor for Measuring Sarcosine. 10th Annual National McNair Scholars Research Conference, Newark, Delaware.
  • Ward, K., Y. Zhang, S. Burrs, M. Shupler, D. Jaroch, J.L. Rickus, E.S. McLamore (2012) A Hollow Fiber Membrane Bioreactor for Treating Household Graywater: Challenges and opportunities. Silver Nanoparticles in Graywater: Effect on Microbes in a Hollow Fiber Membrane Bioreactor. University of Florida Undergraduate University Scholar Symposium, UF Center for Undergraduate Research.
  • Neal, A., S. Burrs, E.S. McLamore (2012) Encapsulation of biofilms on hollow fiber membranes for decentralized graywater treatment. 17th Annual Institute of Biological Engineering Annual Conference, Indianapolis, IN.
  • Porterfield, M., Chaturvedi, P., and E.S. McLamore (2012) Nanoparticle-mediated detection of cyanotoxins. 17th Annual Institute of Biological Engineering Annual Conference, Indianapolis, IN. Undergraduate Poster Presentation Grand Prize
  • Stensberg, M., E.S. McLamore, G. Yale, Q. Wei, A. Wei, H. Ochoa-Acuna, M.S. Sepulveda, D.M. Porterfield (2012) Non-invasive, real-time methods for the detection of physiological changes in Daphnia magna embryos exposed to ionic silver and silver nanoparticles. 17th Annual Institute of Biological Engineering Annual Conference, Indianapolis, IN.
  • Taguchi, M., P. Chaturvedi, S.L. Burrs, E.S. McLamore (2012) Facile electrocatalytic nanomaterial deposition techniques for amperometric sensors and biosensors. 17th Annual Institute of Biological Engineering Annual Conference, Indianapolis, IN.
  • Taguchi, M., J. Foster, E.S. McLamore (2012). Real time, spatially resolved fiber optic microsensors for studying ocean acidification. UF Marine Biology Symposium. St. Augustine, FL. Best Graduate Poster Presentation
  • Ward, K., Y. Zhang, S.L. Burrs, M. Shupler, E.S. McLamore (2012). A Hollow Fiber Membrane Bioreactor for Treating Household Graywater. 17th Annual Institute of Biological Engineering Annual Conference, Indianapolis, IN.


Progress 10/01/10 to 09/30/11

Outputs
OUTPUTS: Developed novel nanomaterial-mediated biotechnology Data was collected on the development of nanomaterial-mediated biosensors, and graduate and undergraduate students in my lab are working together to prepare a manuscript for submission to a peer review journal. Developed novel water processing biotechnology Built, monitored and tested a novel bioreactor for potential household graywater treatment. Students are currently preparing a document for submission to a peer review journal. PARTICIPANTS: E.S. McLamore was the principal investigator of the project, and was responsible for all project management, lead sensor development efforts, mentored students regarding sensor development, and trained/recruited graduate students for future research. P. Chutaverdi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Mrs. Chutaverdi also started the inaugural UF student chapter of the Institute of Biological Engineering and helped recruit students for future sensor development work. S. Burrs is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. M. Taguchi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, and analytical electrophysiology training. N. Garland is an ABE undergraduate student who took sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Mr. Garland is currently working part time in our laboratory and is primarily focused on cell culture for bioenergy research. K. Ward is an ABE undergraduate student who took sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Y. Zhang is an ABE undergraduate student who took sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. P. Pullammanappallil is an Associate professor in ABE collaborating on the start of an bioenergy project with Dr. Garland on the development and construction of membrane aerated bioreactors for methane extraction. B. Welt is an Associate professor in ABE collaborating on the start of an algal-based bioenergy project. B. Gao is an Associate professor in ABE collaborating on the start of an project investigating the fate and transport of nanosilver in microbial biofilm systems. A proposal was prepared and submitted to NSF on this topic together with Dr. Gao. S. Wilson is an extension specialist at the Walton County Extension Office who assisted in the development of the inaugural Biosensors Summer Camp in collaboration with the 4H summer camps. TARGET AUDIENCES: The target audience has been graduate and undergraduate students, collaborators, co-investigators, and interested/concerned citizens. S. Wilson was responsible for organizing a target audience of K-12 students for the Biosensors camp, which will be lead by PI McLamore. This group was made up of a diverse mix of K-12 students from a variety of social, economic, or educational backgrounds. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Biosensors developed by our research group were used in a number of hypothesis driven research projects, including environmental, agricultural and biomedical research. My research group hosted a K-12 summer camp at UF for teaching youth and primary school educators how to build, design and use biosensors and sensors.

Publications

  • Claussen, J.C., M.S. Artiles, E.S. McLamore, S. Mohanty, J. Shi, J.L. Rickus, T.S. Fisher, D. M. Porterfield (2011). Electrochemical glutamate biosensing with nanocube and snanosphere augmented single-walled carbon nanotube networks: a comparative study. Journal of Materials Chemistry, 21: 11224-11231.
  • Jaroch, D. E.S. McLamore, W. Zhang, J. Shi, J.L. Garland, M.K. Banks, D.M. Porterfield, J.L. Rickus (2011). Cell-mediated Silica Entrapment of Bacterial Biofilms. Biotechnology and Bioengineering, 108(10): 2249-2260.
  • McLamore, E.S., and D.M. Porterfield (2011). Non-invasive tools for measuring metabolism and biophysical analyte transport: Self-referencing physiological sensing. Chemical Society Reviews, 40: 5308-5320.
  • McLamore, E.S., M.C. Stensberg, M.S. Sepulveda, W. Zhang, M.K. Banks, D.M. Porterfield (2011). A self-referencing microelectrode for real time measurements of silver flux. Sensors and Actuators B: Chemical, 153: 445-452.
  • McLamore, E.S., J. Shi, D. Jaroch, C. Barcus, J. Osbourne, A. Uchida, Y. Jiang, K.K. Buhman, M.K. Banks, D. Teegarden, J.L. Rickus, D. M. Porterfield (2011). A self referencing enzyme-based microbiosensor for real time measurement of physiological glucose transport. Biosensors and Bioelectronics, 26(5): 2237-2245.
  • Shi, J., J.C. Claussen, E.S. McLamore, A. ul Haque, D. Jaroch, A.R. Diggs, P. Calvo-Marzal, J.L. Rickus, D.M. Porterfield (2011) A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors. Nanotechnology, 22: 1-10.
  • Shi, J., E.S. McLamore, D. Jaroch, J.C. Claussen, J.L. Rickus, D.M. Porterfield (2011). Oscillatory glucose flux in INS 1 pancreatic β cells: A self-referencing microbiosensor study. Journal of Analytical Biochemistry, 411(2): 185-193.
  • Stensberg, M., Q. Wei, E.S. McLamore, D.M. Porterfield, A. Wei, M.S. Sepulveda (2011). Toxicological Studies on Silver Nanoparticles: Challenges and Opportunities in Assessment, Monitoring and Imaging. Nanomedicine, 6(5): 879-898.
  • Wan, Y., E.S. McLamore, L. Fan, H. Hao, D.M. Porterfield , Z. Zhang, W. Wang, Y. Xu, J. Lin (2011). Non-invasive measurement of real-time oxygen flux in plant systems with a self-referencing optrode. Nature Protocol Exchange, doi:10.1038/protex.2011.266.
  • Zheng, W., E.S. McLamore, D.M. Porterfield, S. Donkin, D. Teegarden (2011). Effect of 1,25 dihydroxyvitamin D on energy metabolism in MCF10A breast epithelial cells. Experimental Biology 2011, Washington, DC.
  • McLamore, E.S., D. Marshall Porterfield, R.B. Borgens, M.K. Banks (2011). Self-referencing luminescent optrodes for non-invasive, real time measurement of extracellular flux. Proceedings of SPIE-International Society of Optical Engineering. Orlando, FL. Paper No. 8025:802505.
  • Sun, X., E.S. McLamore, D.M. Porterfield, and O. Akkus (2011). Calcium efflux from bone matrix in response to mechanical loading. Proceedings of the American Society of Mechanical Engineering Bioengineering conference. SBC2011-53625.


Progress 01/01/10 to 09/30/10

Outputs
OUTPUTS: 1) Preliminary data was collected on the development of novel based methanol biosensors using combinations of immobilized alcohol oxidase enzymes and nanomaterials such as multiwalled carbon nanotubes and amorphous platinum nanoclusters. 2) Seminars were given to various societies and courses on campus to gain student interests in the field of biosensors, including: The American Society of Agricultural and Biological Engineering (ASABE), Engineering in Medicine and Biology Society (EMBS), and Introduction to Biomedical Engineering (BME1008). 3) Presented research at the Annual conference for the American Society of Gravitation and Space Biology (ASGSB), Institute of Biological Engineering (IBE), and Society for Optical Engineering (SPIE). Chaired sessions at the IBE and SPIE meetings. 4) A Biosensors Summer Camp was developed in collaboration with the Walton County 4H Extension Office. The camp is to be held July 25-29 2011 in Frazier Rogers Hall. PARTICIPANTS: E.S. McLamore was the principal investigator of the project, and was responsible for all project management, lead sensor development efforts, mentored students regarding sensor development, and trained/recruited graduate students for future research. P. Chutaverdi is an ABE graduate student who underwent sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Mrs. Chutaverdi also started the inaugural UF student chapter of the Institute of Biological Engineering and helped recruit students for future sensor development work. N. Garland is an ABE undergraduate student who took sensor development training, chemical and biological hazard training, cell culture training, and analytical electrophysiology training. Mr. Garland is currently working part time in our laboratory and is primarily focused on cell culture for bioenergy research. J. Garland is the Chief Life Scientist at NASA's Kennedy Space Center (Life Science Laboratory), and has been involved in collaborating on the development and construction of membrane aerated bioreactors for methane extraction. P. Pullammanappallil is an Associate professor in ABE collaborating on the start of an bioenergy project with Dr. Garland on the development and construction of membrane aerated bioreactors for methane extraction. B. Welt is an Associate professor in ABE collaborating on the start of an algal-based bioenergy project. B. Gao is an Associate professor in ABE collaborating on the start of an project investigating the fate and transport of nanosilver in microbial biofilm systems. A proposal was prepared and submitted to NSF on this topic together with Dr. Gao. S. Wilson is an extension specialist at the Walton County Extension Office who assisted in the development of the inaugural Biosensors Summer Camp in collaboration with the 4H summer camps. TARGET AUDIENCES: The target audience has been graduate and undergraduate students in terms of recruiting researchers for future work. S. Wilson was responsible for organizing a target audience of K-12 students for the Biosensors camp, which will be lead by PI McLamore. This group was made up of a diverse mix of K-12 students from a variety of social, economic, or educational backgrounds. PROJECT MODIFICATIONS: n/a

Impacts
1)Developed a new technique for quantifying distributions of suspended cells in collaboration with the Space Life Sciences Laboratory at NASA's Kennedy Space Center and Purdue University. We are also including this information as part of a peer reviewed manuscript currently in preparation. Prepared and will submit an equipment proposal requesting funds for the developed technology to be used by researchers at UF. 2)Based on networking at annual IBE, ASGSB, and SPIE conferences, I developed new concepts for enhancing our current biosensor development protocol. The conceptual designs were discussed during group research meetings with graduate students at UF, and we are now testing the conceptual ideas for implementation into novel sensor designs. If proven successful, these conceptual ideas will significantly enhance the biosensors we are currently developing. 3)Attended grant writing workshops at USDA/NIFA and NSF. These grant writing workshops provided a wealth of information regarding the vision of program directors, and also the future direction of the agencies. Networked during these workshops and met numerous colleagues in the area of biosensors, and also colleagues interested in collaborating. A proposal is currently being prepared in response to a recently released RFA by USDA. I also prepared and submitted a multi-university proposals to the NSF on which I was the PI. 4) Developed a new course entitled "Fundamentals and Applications of Biosensors" to be taught in Spring 2011 within the ABE department. The curriculum committee is currently reviewing the course for potential addition to the UF course catalogue. This course will be foundation for teaching students about the fundamental principles of Biosensors, and will train the next generation of researchers the importance of biosensors in the areas of agricultural and environmental research. 5) In response to a RFP by the UF Honors Program, I developed a new interdisciplinary course entitled "Visualization of Biological and Biomimetic Processes". The inaugural course is to be taught in collaboration with the Department of Art & Art History in Spring 2012. This interdisciplinary course will provide ABE students with a unique skillset in terms of displaying quantitative information, and in the future will be incorporated into existing extension programs for disseminating research to public audiences. 6) Developed, tested and validated a new sensor for measuring free silver in liquid samples. Submitted a manuscript to a peer reviewed journal, and we are currently using this data to support two proposals currently in preparation to be submitted to the Florida Space Grant Consortium.

Publications

  • Peer reviewed journal articles McLamore, E.S., Zhang, W., D.M. Porterfield and M.K. Banks (2010). Real time, non-invasive biofilm physiology during chemical toxin exposure. Environmental Science and Technology. 44: 7050-7057.
  • McLamore, E.S., D. Jaroch, M.R. Chatni, and D.M. Porterfield (2010). Self-referencing optrodes for measuring real time oxygen flux in plant roots and photosynthetic microbial mats. Planta, 232(5): 1087-1099.
  • McLamore, E.S., A. Diggs, P. Calvo Marzal1, J. Shi, J. Claussen, A. Murphy, D.M. Porterfield (2010). Non-invasive quantification of endogenous root auxin transport using an integrated flux microsensor technique. Plant Journal, 63: 1004-1016.
  • McLamore, E.S., S. Mohanty, J. Shi, J.L. Rickus, and D.M. Porterfield (2010). Real time neuronal glutamate flux during potassium stimulation. Journal of Neuroscience Methods, 189: 14-22.
  • Prather, K.J. E.S. McLamore, D.M. Porterfield (2010). Synthetic Biology and the Rational Design of Microbial Chemical Factories. Proceedings of the 26th Annual American Society of Gravitational and Space Biology, 24(1).
  • McLamore, E.S., M. Stensberg, G. Yale, H. Ochoa-Acuna, M. Sepulveda, X. Sun, O. Akkus, and D.M. Porterfield (2010). An imaging technique for monitoring real time changes in morphology within the cell, tissue, or whole organism spatial domain. Proceedings of SPIE-International Society of Optical Engineering, Defense, Security, and Sensing Conference, Orlando, FL. 7674-14.
  • Proceedings McLamore, E.S., G. Yale, H. Ochoa-Acuna, D.M.Porterfield, M. Stensberg and M.S. Sepulveda (2010). Use of Real-time Biological Sensing for Evaluating Mechanisms of Toxicity of Silver Nanoparticles. Environmental Effects of Nanoparticles and Nanomaterials: 2010. Clemson, SC.
  • M.S. Sepulveda, M. Stensberg, G. Yale, H. Ochoa-Acuna, D.M. Porterfield, and E.S. McLamore (2010). Nanosilver vs. ionic silver in Daphnia embryos: Using Real-time Biological Sensing for Testing Differences in Toxicity. 31st Annual Meeting of the Society of Environmental Toxicology and Chemistry, Portland, OR.
  • McLamore, E.S., J. Shi, J. Claussen, J.L. Rickus, and D.M. Porterfield (2010). A self-referencing glutamate biosensor for measuring real time neuronal glutamate flux. Institute of Biological Engineers, Annual Meeting. Cambridge, MA.