Source: Eldertide LLC submitted to
DEVELOPING ELDERBERRIES AS A SPECIALTY CROP BY SUPPORTING A GROWERS` NETWORK AND BY CONDUCTING PROTOTYPE DEVELOPMENT OF NUTRACEU
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0222786
Grant No.
2010-33610-21408
Project No.
MEK-2010-02118
Proposal No.
2010-02118
Multistate No.
(N/A)
Program Code
8.12
Project Start Date
Sep 1, 2010
Project End Date
Aug 31, 2014
Grant Year
2010
Project Director
Johnston, E.
Recipient Organization
Eldertide LLC
555 Gardiner Road
Dresden,ME 04342
Performing Department
(N/A)
Non Technical Summary
US federally funded research and international research is connecting elderberry (S. nigra and S. canadensis) to the treatment of diabetes, cancer, heart disease, AIDS, herpes simples virus HSV-1 and influenza types A and B. Many of these studies have focused on the presence of high levels of antioxidants present in the berries of these perennial shrubs. Today, consumers understand the important role antioxidants play in human health and value of local and organic agricultural products. Virtually all elderberry-based nutraceutical products available in the United States rely on imported Sambucus nigra (the European elderberry). Retail sales of three imported elderberry syrups are among the top 10 of 662 herbal supplements available for adults. In 2008, Austria, a country the size of Maine, produced 19,200,000 pounds of elderberries. [Even if Maine were to meet just 5% of Austria's current elderberry production, more than $2.2 M USD could be generated for Maine's small and mid-sized farms.] To date, the US has not recognized the opportunity to develop commercial scale production of elderberries for national and international markets. As a result, two major issues can be identified: 1) US manufacturers of elderberry-based products are dependant on imported raw product due to inadequate domestic supply; 2) imported raw products in the form of juice concentrates do not carry forward the full phytonutrient profiles of the raw fruit due to degradation during processing resulting in inferior products. This Phase II project seeks to capitalize on an opportunity to provide increased revenue for small and mid size farms by supporting domestic elderberry production for high-value niche markets thus significantly decreasing manufacturers' dependence on imported elderberry products and increase competitiveness by offering consumers superior US produced elderberry products for national and international markets. Phase II has two objectives: Objective 1: Establish a reliable supply of US grown elderberries by supporting a growers' network of small and mid-size farms for commercial scale organic elderberry production. Objective 2: Conduct prototype development of nutraceutical products utilizing US grown elderberries. This project will apply innovative processing technologies to previously identified superior varieties of US grown organic elderberries. Anticipated Phase II results are increased US elderberry production supported by a growers' network, and Phase III commercialization of two organic elderberry-based nutraceuticals for national and international distribution. At the completion of Phase II, Eldertide will be well positioned to introduce a full line of elderberry-based nutraceutical products that leverage rapidly growing consumer interest to provide substantial income opportunities for small and mid-size farms.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2041129108025%
2041129106025%
5022239200050%
Goals / Objectives
Objective #1: Establish a reliable supply of US grown elderberries by supporting a growers' network of small and mid-size farms for commercial scale production. A network of growers will be supported by outreach and education to enable them to develop the necessary skills for domestic production of elderberries as a specialty crop for high-value niche markets. Efforts will focus on training and developing skills prior to planting and cultivation of the four superior varieties of elderberries identified in Phase I. Technical questions: 1. What are the perceived barriers to growing elderberries as a specialty crop for new growers and for existing growers 2. What resources need to be made available for growers relative to financing agricultural infrastructure and facilitating connections to new and existing support systems Expected outputs: Participants will receive comprehensive education and training to grow elderberries as a specialty crop. Presentations will be archived for future reference and use. Selected participants will qualify for further support, offered post Phase II through Eldertide LLC and its strategic partners. Objective #2: Conduct prototype development of nutraceutical products utilizing US grown elderberries. This project will apply innovative processing technologies to previously identified superior varieties of US grown organic elderberries. Technical questions: 1. What effect do different juicing processes have on antioxidant retention, as determined by Total Antioxidant (TA) and Total Phenolic (TP) assays and HPLC profiles 2. When compared to the existing elderberry syrup (anthoimmune), does the elderberry syrup developed in Phase II demonstrate statistically significant improvements based on analysis of chemical, microbiological and sensory evaluation data 3. Does the new nutraceutical product developed in Phase II carry forward serving equivalent levels of antioxidants and demonstrate levels of safety and efficacy equal to the Phase II improved elderberry based on analysis of chemical and microbiological data 4. Does the new nutraceutical product developed in Phase II indicate above average overall consumer acceptance based on sensory evaluations data Expected Outputs: A prototype of one improved nutraceutical syrup and one new nutraceutical product.
Project Methods
Task 1: Support a growers' network of small and mid-size farms to develop the necessary skills for US commercial production of elderberries as s specialty crop for high-value niche markets. The methodology for Task 1 is based on experiential learning at a demonstration orchard, classroom instruction, farm visits, multi-media presentations, and follow-on access to information. Providing support for a growers' network will begin by preparing course descriptions, writing the curriculum and publishing materials for Eldertide's Elderberry School (EES). Following solicited participation in EES, Eldertide will host an initial meeting of growers. Utilizing the expertise of consultants and resources, the PI and Eldertide's horticultural liaison will create lesson plans based on class needs. Experiential learning, demonstrations and direct instruction will be the methods of instruction. The classes will conclude with a published plan for continued support. Task 2: Develop prototypes for one new elderberry-based nutraceutical product and one improved elderberry-based nutraceutical based on the blended juice formula developed in Phase I. The methodologies for Task 2 are based on standard microbiological and chemical quantitative tests assays conducted at the University of Maine along with qualitative sensory evaluations utilizing focus groups conducted at Oregon State University's Food Innovation Center. This project begins with a comparative evaluation of three juicing methods to determine the process most suitable for nutraceutical product development. Steam extraction, hand crank pressing and hydraulic pressing methods will be evaluated. The experimental design for each juicing method will be a 3 x 3 x 3 factorial study with each extraction method replicated three times and three samples being analyzed for each replicate. Data will be used for formulation of nutraceutical prototype products. Juice samples and dried powders created from those juices will be assayed for chemical attributes including total monometric anthocyanins and total phenolics and microbiological safety and stability will be determined for juices, powders and samples of new and improved nutraceutical prototype products. Sensory Evaluations will be conducted for three versions of a new elderberry-based nutraceutical product and for three versions of an improved elderberry-based nutraceutical product. Methodology will utilize Preference Acceptance Tests and Focus Group Sessions. Task 1 outputs will be evaluated for impact by an increase in Maine's commercial elderberry production as determined by acres in production and annual yields. Task 2 outputs will be evaluated for impact by prototype products demonstrating superior phytonutrient profiles as determined by chemical assays, along with verification of microbiological safety and stability, and consumer interest as indicated by results of sensory evaluations.

Progress 09/01/10 to 08/31/14

Outputs
Target Audience: Eldertide established an elderberry growers' network by providing training and support for selected farmers to grow elderberries for sale to a variety of value added product producers. The network consists of individuals and small farms that are looking to grow a perennial crop that has potential for creating value added products and for sale to Maine Medicinals for nutraceuticals. This has resulted in the early stage growth of an organic, domestic supply of US grown, superior grade elderberries. Members of the growers network are now providing raw product to local manufacturers of nutraceuticals and fermented beverage sectors. Future applications resulting from the success of the Phase II project include expanding the grower's network from artisanal growers to mid-size farms growing elderberries and other anti-oxidant rich berries. Changes/Problems: This project utilized a micro powder as an intermediate product to retain maximum levels of phytonutrients. The process produced a stable micro powder that provided superior retention of phytonutrients, flavor, color and aroma. Eldertide used this product in prototype product development. Eldertide then developed three prototype elderberry lozenges.The results of the lozenge development component of Phase II determined that although it is technically feasible to produce an elderberry lozenge with superior phytonutrient capacity, the high cost of purchasing the equipment and processing the raw materials needed to produce the products is not economically viable at this time. What opportunities for training and professional development has the project provided? Education and training were given to participants in the Eldertide Elderberry School as stated above. Efforts focused on training and developing skills prior to planting and cultivation of the four superior varieties of elderberries identified in Phase I. How have the results been disseminated to communities of interest? Eldertide and its Phase I and II accomplishments was one of five companies highlighted in an SBA Federal And State Technology Partnership effort as a Success Story by the Maine Technology Institute as an example of a company that has achieved commercialization of R&D efforts based on the SBIR Program. Ms. Johnston regularly gives talks at the Maine Organic Farmers and Grower's Association Common Ground Fair. Farm visits ocurred. The company was featured as a LARTA Success Story and the company received an SBA Tibbetts Award as well. All publicity is based on the work that Eldertide conducted under the SBIR Phase I and II programs. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Technical Objective 1: Establish a reliable supply of US grown elderberries by supporting a growers' network of small and mid-sized farms for commercial scale production. Specific tasks and activities to meet this objective included: a) Development of the EES: The EES is well underway with participants in the growers' network receiving opportunities for experiential learning, demonstrations, traditional classroom instruction, and workshops. Twelve farms were selected from over forty applications. Members of the first class are very diverse in terms of their knowledge of farming, their familiarity with elderberries, and the size and focus of their farms. However, they all share an interest in growing elderberries commercially for various markets. Development of course description, curriculum and prepared educational materials for students The EES contracted with CEI, Inc. to assist in developing the curriculum and implementing the program. CEI is focused on improving the long term viability and sustainability of environmentally focused, natural resource based industries. CEI in association with the Maine Department of Agriculture's Food and Rural Development Office oversees Maine's Farms for the Future program, a state-wide economic development program that targets farms transitioning to a new generation or start-up farming operations. The participants were selected via an extensive application and review process. Participation in the EES provided farmers with access to various business development and support programs at the state and federal levels that they may not have been aware of or participated in previous to their involvement in the growers' network. The curriculum for the EES encompasses four areas associated with the culture and horticulture of elderberries: 1. Plant management from site selection through harvest and propagation 2. Processing and food safety 3. Marketing 4. Business planning, Maine Organic Growers and Farmers Association (MOGFA) certification and insurance Classes were scheduled monthly with farm visits, on-line and phone support available. The project's PI, horticultural liaison, and outside consultants lead workshops that focus on specific areas of expertise necessary to develop a successful elderberry operation. Specific workshops included: 1. A general overview of the industry including basics of organic certification, site selection, disease and pest management, production, harvesting, and processing. 2. Business Planning and Marketing: The Maine SBDC provided comprehensive business management assistance, training, and information to the growers association.This session featured a certified Financial Consultant and several Maine SBDC business counselors that have provided follow-on services and assistance to members of the growers' network. 3. Tips and techniques for elderberry propagation, site selection, and organic certification with farm visits. David Closen from MOGFA provided his expertise and experience in these areas to participants. MOGFA continues to be a valuable resource for information on organic growing and crop certification practices. 4. A discussion on food safety, regulations and value-added products. This session was lead by Beth Calder, PhD Associate Professor of Food Safety and Human Nutrition at UMaine. 5. Individual Farm Visits were scheduled to review farm layout, design, soil conditions, and cultivation and propagation planning. The PI lead these visits accompanied by Eldertide's horticultural liaison. 6. Farm Days at Eldertide Farm with extension and continuing education options as requested by various members of the growers' network. Farm Days allow network participants to focus on individual challenges or share information with other participants on their experiences to date. In meeting this Technical Objective, Eldertide reviewed and addressed perceived barriers to growing elderberries as a specialty crop for new and existing growers. It also identified resources critical to financing agricultural infrastructure and aligned growers to existing business development and economic development resources available in the State of Maine. The selection process for the growers' network was very thorough, although time consuming, to carefully select each candidate. A copy of the application will be made available on Eldertide's website as we expand the growers' network beyond the initial 12 farms. The application may serve as a valuable resource for other states that wish to develop a growers' network to support specialty crop production for high value niche markets in the US. As market demand for value-added products increases, expanding raw elderberry product will necessitate mid-sized farms developing larger scale elderberry orchards. Technical Objective #2 - Conduct prototype development of nutraceutical products utilizing US grown elderberries. Specific tasks and activities to support this technical objective: This project applied innovative processing technologies to previously identified superior varieties of US grown organic elderberries. The Phase II technical objective was to bring one improved nutraceutical syrup and one new lozenge to market. Based on the superior blended elderberry juice developed in Phase I, Phase II began with a comparative evaluation of juicing processes. Task 1 evaluated three juicing methods to determine the process most suitable for nutraceutical prototype product development. Juicing followed manufacturer's instructions for each method. Preliminary data was recorded and analyzed to determine the most appropriate juicing method for Phase II work. Juice samples were analyzed for their microbiological attributes including: total aerobes, yeasts, molds, coliforms, E. coli, and Staphylococcus aureus. Microbiologists at UMaine's Department of Food Science and Human Nutrition and Katahdin Analytical Laboratories conducted assays. The data was utilized for lozenge formulation. Task 3 assayed juice samples for chemical attributes. Elderberry powders were analyzed for total monometric anthocyanins and total phenolics to inform prototype development of powders for Phase II product development. The results of the lozenge development component of Phase II determined that although it is technically feasible to produce an elderberry lozenge with superior phytonutrient capacity, the high cost of purchasing the equipment and processing the raw materials needed to produce the products is not economically viable at this time. Phase II efforts focused on developing five improved elderberry syrups utilizing the identified juicing processing technology from Phase I and work performed early in the Phase II project. Syrups were assayed for microbiological (Katahdin Analytical) and chemical (UMaine's Department of Food Science and Human Nutrition's Microbiology Department) attributes. Analytical chemical assays were conducted by the Linus Pauling Institute. Sensory evaluations were conducted by the Oregon State Univeristy Food Innovation Center for consumer response testing utilizing PAT and Focus Group Sessions. Eldertide, LLC has successfully developed the five new organic nutraceutical products listed below utilizing US grown elderberries under the Maine Medicinal's AnthoImmune™ line: 1) An improved elderberry syrup 2) A children's elderberry syrup formula 3) A classic alcohol and water based tincture 4) A glycerite tincture 5) Micro-powder based hard, soothing lozenges (a product that is technically feasible but not economically viable at this time)

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