Source: PURDUE UNIVERSITY submitted to NRP
MIDWEST CENTER FOR BIOENERGY GRASSES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0222353
Grant No.
2010-34328-21047
Cumulative Award Amt.
(N/A)
Proposal No.
2010-01616
Multistate No.
(N/A)
Project Start Date
Jul 1, 2010
Project End Date
Jun 30, 2013
Grant Year
2010
Program Code
[DD-E]- Midwest Center for Bioenergy Grasses, IN
Recipient Organization
PURDUE UNIVERSITY
(N/A)
WEST LAFAYETTE,IN 47907
Performing Department
Botany & Plant Pathology
Non Technical Summary
The Midwest Center for Bioenergy Grasses (MCBG) will provide a national resource of research and education dedicated to optimizing bioenergy crops for their end-use in biofuels production. Our driving mission is to take the discoveries made in our laboratories into agronomic practice in a sustainable and environmentally sound way and to develop the technologies for optimal conversion of bioenergy grasses to biofuels and other value-added materials. We will evaluate various genetic resources of biomass sorghum) as energy crops. We will use recombinant inbred lines of intermated B73 x tropical maize landraces to identify quantitative trait loci associated with biomass quantity and quality traits. We will establish test plots of tropical maize hybrids and biomass sorghum with perennial grasses Miscanthus x giganteus and switchgrass at Purdue's Water Quality Field Station to accurately determine the NPK status important for long-term sustainability. We will integrate and test novel production systems for economical extraction of soluble sugars and biomass during harvest.
Animal Health Component
40%
Research Effort Categories
Basic
40%
Applied
40%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1022030108110%
1022099108110%
2032030108130%
2042099108130%
4022030108110%
4022099108110%
Goals / Objectives
The MCBG comprises four Thrust Areas: 1) Grass Genetics for improvement of feedstock quantity and quality, 2) Biomass Biology for optimizing biomass architecture for end use, 3) Sustainable Cropping Systems for plant production optimized for sustainability and cost-efficiency, and 4) Biofuels Engineering for development of direct-conversion technologies for scalable and distributive hydrocarbon refineries. Scientists representing each of these four Thrust areas have coordinated to design an inaugural program comprising three projects that will evaluate various genetic resources of tropical maize and biomass sorghum as energy crops. We will test productivity of both biomass and sugar content on a variety of soil types and fertilizer regimens, and integrate production systems for economical extraction of sugar during harvest. We will also establish test plots with perennial grasses at Purdue's Water Quality Field Station to accurately determine the NPK status important for long-term sustainability in side-by-side comparisons with perennial crops. Our goal in subsequent years is to evaluate the potential for a three-year crop rotation of grain corn to biomass sorghum to soybeans that will provide farmers with new options for the sustainable production of both grain and an energy crop. We have already engaged growers, biofuel producers, and implement companies to work with members of our center to test these crops in real-life situations in the 2010 season.
Project Methods
Three integrated projects are proposed that involve both field and lab experiments. In Project I, we will determine previously unmeasured biomass growth characteristics, such as stem diameter, for for biomass and sugar formation, in maize founder lines, their recombinant inbred lines, and biomass sorghum hybrids; we will combine these with existing, relevant datasets such as plant height and flowering time. Soluble sugar, cellulose and Klason lignin will be assayed by standard lab procedures, and selected variants will be assayed for distribution of hexose, pentose, and aromatic types by high-throughput Pyrolysis-Molecular Beam MS. Project II will be conducted at Purdue University's Water Quality Field Station (WQFS) and at regional Purdue Agriculture Centers (PACs). WQFS treatments will include a low-input big bluestem: a facsimile for the native prairie community with no fertilizer inputs, maize grown in rotation with soybean and fertilized optimally for local conditions, continuous maize fertilized optimally for local conditions with no residue removal, continuous maize fertilized optimally for local conditions with residue removal at harvest, Miscanthus production using best known management practices for establishment and maintenance, switchgrass production using best known management practices for establishment and maintenance, sorghum and tropical maize production using a unique highly yielding, high sugar and high biomass genetic material. Intensive plant tissue, soil, drainage water, and surface gas flux sampling campaigns will be conducted to quantify pools and fluxes of water, C and N throughout the growing season. Several calculations will permit us to compare the production efficiencies of these biomass cropping systems in the context of the water, N, and C economies, the contrasting composition of the biomass per se, and the mass losses of C and N to water and the atmosphere. Various multivariate statistical approaches will then be applied to measured and calculated parameters to determine significant differences among candidate biofuel systems. In Project III we will simulate the harvesting and processing of tropical maize hybrids and sweet sorghum cultivars using the three harvesting approaches outlined. These approaches will be: 1) harvesting whole plant as silage; 2) harvesting plant fractions - leaves and stalks; and 3) shred and extract juice in-field during harvest. These technologies will be applied to test plots of the tropical maize hybrids and sweet sorghum cultivars. The data collected will be used to develop engineering roadmaps and logistical pathways for simulating commercial scale production of these biofuel crops.

Progress 07/01/10 to 06/30/13

Outputs
Target Audience: The target audience is the research community of plant biology at large, with special focus on the rising community of researchers working on plants and bioenergy. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? This study included interdisciplinary training in field and laboratory work for undergrads, a graduate student and a technical assistant. How have the results been disseminated to communities of interest? Results of our study are currently been written for publication in a journal devoted to bioenergy feedstock production. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We completed a multi-year study to evaluate performance of sweet and biomass sorghum as an alternative lignocellulose feedstock for conversion to biofuels. Studies included an evaluation of nitrogen demand. The 2012 growing season provided an unexpected environmental challenge of severe drought to compare performance of the same genotypes to yields in years with normal rainfall. We established that all sorghum genotypes yielded equivalent biomass under drought conditions as in normal rainfall, whereas a tropical biomass maize suffered severe limitations of yield. We established that all biomass sources performed best on little or no added N, and that high N reduced yield of lignocellulosic biomass.

Publications


    Progress 07/01/11 to 06/30/12

    Outputs
    OUTPUTS: Our objective was to repeat a determination of the impact of nitrogen fertilizer rate on yield and composition of diverse lines of sorghum and control maize inbreds and hybrids. This was particularly interesting year due to the severe drought that plagued the Midwest for most of the summer. Results from the previous year and others from the literature resulted in a Perspective article that envisions how biomass sorghum is a logical choice for a dedicated energy crop in the Midwest. Several participants spoke of results at scientific meetings. PARTICIPANTS: The project involved two research technicians, Nick Babcock (Weil) and John Klimek (Carpita), and a graduate student, Baron Hewettson (Mosier). Several undergraduate interns also assisted in sampling and harvests. TARGET AUDIENCES: Target audiences for our results are bioenergy, researchers, engineers and economists along the field to biofuel pipeline. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

    Impacts
    Several sweet sorghum cultivars, a photoperiod-sensitive biomass sorghum, and a tropical maize hybrid were grown, under conditions of 0, 60, and 180 lbs/acre additional nitrogen. Despite the severe drought, the sorghum cultivars yielded sugar and total biomass that equaled or in some cases exceed yields of 2010, a year of near average summer rainfall. In contrast, yields of the tropical maize hybrid, M81E, which was second highest in yield in 2010, exhibit drastic reductions in yield. As in 2010, highest yields were found with either 0 or 60 lbs/acre N, and in all cultivars, 180 lbs/acre led to biomass yield losses. A publication that summarizes the project results is in preparation.

    Publications

    • No publications reported this period


    Progress 07/01/10 to 06/30/11

    Outputs
    OUTPUTS: Our objective was to repeat a determination of the impact of nitrogen fertilizer rate on yield and composition of diverse lines of sorghum and control maize inbreds and hybrids. However, early field flooding necessitated a replanting in late May, and in early August we were struck by a massive hailstorm that destroyed the entire experiment. Results from the previous year and others from the literature resulted in a Perspective article that envisions how biomass sorghum is a logical choice for a dedicated energy crop in the Midwest. PARTICIPANTS: The project involved two research technicians, Suzanne Cunningham (Volenec)and Nick Babcock (Weil), two graduate students, Isaac Emery and Baron Hewettson (Mosier). TARGET AUDIENCES: Target audiences for the Perspective article are researchers, engineers and economists along the field to biofuel pipeline. PROJECT MODIFICATIONS: Our plots were destroyed by hailstorm, and we need to repeat the field experiment in 2012 to validate the results from 2010.

    Impacts
    Personnel efforts were redistributed to other projects, and unexpended funds were held in reserve for a retrial for 2012. We will be requesting a no-cost extension to permit the retrial to validate results from the previous year.

    Publications

    • Dweikat, I., Weil, C.F., Moose, S.P., Kochian, L., Mosier, N.S., Ileleji, K.E., Brown, P.J., Peer, W.A., Murphy, A.S., Taheripour, F., McCann, M.C., Carpita, N.C. (2012) Envisioning the transition to a next-generation biofuels industry in the Midwest. BioFPR, In press.