Progress 12/01/09 to 09/30/14
Outputs
Target Audience: Nematologists, plant pathologists, soybean breeders, agricultural professionals, extension educators, and soybean growers Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest? The results have been provided to soybean breeders for selecting sources of SCN-resistance for breeding SCN-resistant soybean cultivars. The results have been provided to regional extension educators in Minnesota for their extension educations. The results have been presented at soybean grower meetings and field tours, and scientific professional meetings (see the conference abstracts). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
(1) Impact of cultivar resistance on virulence phenotypes of the soybean cyst: Field plot experiment was established in Waseca in 2003 to determine the effect of different sequences of SCN-resistance sources (PI 887888, Peking, and PI 437654) on dynamics of SCN population densities and their virulence phenotypes (races or HG Types). The original population in the field was HG Type 0 (race 1). Among the three SCN-resistant cultivars, the cultivar carrying PI 88788 resistance supported highest SCN egg population density, the cultivar carrying Peking resistance intermediate, and cultivar carrying PI 437654 resistance supported lowest SCN egg population density. Based on the results from the site during 2003 to 2012, SCN Populations selected by cultivar with PI 88788 source of resistance can only overcome the resistance of PI 88788 not the other two, and Peking-selected SCN populations can overcome the resistance in Peking, not each other. PI 437654 can select SCN populations to overcome both sources of resistance PI 88788 and Peking. (2) Identification of SCN-resistance in soybean germplasms: Seventeen soybean cultivars and accessions that were reported resistant to HG Type 1- (race 14) or/and HG Type 2- (race 1) were tested against 13 different nematode populations including race 1 (HG Type 2-), race 2 (HG Type 1.2-), race 3 (HG Type 0-), race 4 (HG Type 1.2.3-) and race 14 (HG Type 1-) collected from Minnesota. Most of the lines tested had high or moderate resistance to race 1, race 2 and race 3 populations and can serve as an alternative resistance sources to PI 88788. However, most of the lines were susceptible to race 4 and the two race 14 nematode populations tested. Only PI 633736 has a high level of resistance to all the nematode populations used. PI 417091, PI 404166, PI 567516C, PI 629013 have moderate or high resistance to race 4 and at least one of the two race 14 populations. (3) Identification of SCN-resistance QTLs: The QTLs conferring resistance to an HG Type 2.5.7 population (race 1) of SCN were investigated by analyzing genomes and phenotypes of SCN resistance of 184 MN0095 x PI 567516C F2:3 families. A total of 1536 SNP markers were analyzed for their association with SCN-resistance QTLs. This study revealed that the major QTLs conferring SCN-resistance in PI567516C are distinct from the QTLs in PI 88788, Peking, and many other SCN-resistance sources. Two QTLs located on chromosomes 10 (LG O) and 8 (LG A2) in PI 567516C were identified to be resistant to SCN. The QTL on chromosome 10 was the major one with 32.0% additive heritability and 68.7% of the genetic variance of resistance to HG Type 2.5.7, and the closest SNP marker was BARC-008021-00209. The QTL on chromosome 8 displayed 6.8% additive heritability and 14.8% of the genetic variance of resistance to HG Type 2.5.7 and the closest SNP marker was BARC-028207-05794. The two SNP markers can be used to select SCN-resistance from PI 567516C in soybean breeding through marker-assisted selection.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Lian, L., Wang, F., Denny, R., Schaus, P., Young, N., Orf, J., and Chen, S. 2014. Soybean cyst nematode resistance in PI 567516C soybean: A promising new source of resistance. Journal of Nematology 46:193.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Bao, Y., Chen, S., Denny, R., Kurle, J. E., Meinhardt, C., Nyuyen, H. T., Orf, J. H., Tiffin, P., Vuong, T., and Young, N. D. 2014a. Implementing association mapping and genomic selection in soybean breeding programs. 15th Biennial Conference Molecular & Cellular Biology of the Soybean: 22.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Bao, Y., T. Vuong, C. Meinbardt, R. Denny, H. T. Nguyen, S. Chen, P. L. Tiffin, J. Orf, and N. D. Young .2014b. Association mapping and genomic selection for soybean cyst nematode resistance. PLant and Animal Genome, The Largest Ag-Genomics Meeting in the World. Web/URL: https://pag.confex.com/pag/xxii/webprogram/Paper12449.html.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Bao, Y., Vuong, T., Meinhardt, C., Tiffin, P., Denny, R., Chen, S., Nguyen, H. T., Orf, J. H., and Young, N. D. 2014c. Potential of association mapping and genomic selection to explore PI88788 derived soybean cyst nematode resistance. The Plant Genome 7 (3): doi10.3835/lantgenome2013.11.0039.
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Progress 01/01/13 to 09/30/13
Outputs
Target Audience: Nematologists, plant pathologists, soybean breeders, agricultural professionals, extension educators, and soybean growers Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest? The results have been presented at the Society of Nematologists annual meeting and used for extention education in Minnesota What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Objective 1. Integrate resistant cultivars with other nematode management tactics to reduce selection pressure on nematode populations and communities. Impact of cultivar resistance on virulence phenotypes of the soybean cyst nematode: A field plot experiment was established in Lamberton, Minnesota in 2008 to determine the effect of different sequences of SCN-resistance sources (PI 887888, Peking, and PI 437654) on dynamics of SCN population densities and their virulence phenotypes (HG Types or races). The site was initially infested by SCN race 1 (HG Type 2.5.7) that can overcome PI 88788 resistance, the source of resistance of majority commercial resistant cultivars. After three years, only the monoculture of Pioneer 91M90 carrying Peking resistance significantly increased FI on the source of resistance Peking, resulting in change of the SCN population from HG Type 2 to HG Type 1,2 which can overcome the resistance in both Peking and PI 88788. Objective 2. Identify and incorporate new sources of resistance into elite cultivars Breeding: Several soybean lines carrying SCN-resistance different from the sources PI 88788 and Peking were used for breeding SCN-resistant cultivars. Crosses were made in 2009, 2010, 2011, and 2013. The progenies of the crosses will be evaluated for SCN resistance. Impact: This study will provide useful information for strategically deploying appropriate sources of SCN-resistance for managing SCN.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2012
Citation:
Chen, S. 2012. Impact of tillage and source of resistance on dynamics of the soybean cyst nematode population and its virulence phenotype. Journal of Nematology 44:457.
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Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: A greenhouse experiment was conducted to study the effect of source of resistance on selection of SCN virulence phenotype with the initial SCN populations race 1 (HG Type 2,5,7), race 3 (HG Type 0), and race 14 (HG Type 1,3,6). The soybean sequence treatments include: 1) monoculture of soybean near isogenic line (NIL) without (NIL-S) or with (NIL-R) rhg1 SCN-resistance gene derived from PI 209332; 2) monoculture of either of the three cultivars Latham EX547 RR N, 91M90, and AR5084, carrying SCN-resistance from PI 88788, Peking, and PI 437654, respectively; 3) rotation of either two of the three cultivars; and 4) original population in soil stored at -20C. The soybean was planted in pots consecutively for eight periods each about 3-4 months. After that, all pots were planted to the SCN-susceptible soybean Sturdy to increase population density before they were tested for their reproductive potential on their sources of resistance PI 88788, Peking, and PI 437654. PARTICIPANTS: Principal Investigators: Senyu Chen. Technicians: Cathy Johnson, Wayne Gottschalk. TARGET AUDIENCES: Nematologists, plant pathologists, soybean breeders, agricultural professionals, extension educators, and soybean growers PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
There was no significant difference in Female Index (FI) between the treatments of susceptible soybean and storage at -20C, indicating that planting SCN-susceptible soybean had no detectable selection on virulence phenotype. When the initial avirulent population was race 3, PI 88788-derived cultivar selected a population that overcame PI 88788, and the Peking-derived cultivar selected SCN populations that overcame the resistance in Peking. In contrast, the PI 437654-derived cultivar selected SCN populations that increased FI on both PI 88788 and Peking sources of resistance. The NIL-R selected the population that can overcome PI 88788 and apparently also increased the FI on Peking. When the initial population was race 1 which is virulent to PI 88788, both Peking- and PI 437654-derived cultivars increased the FI on Peking. For the initial population race 14, only PI 88788-derived cultivar and NIL-R selected the populations that overcame PI 88788. No selected population could overcome the resistance in PI 437654. This study will provide useful information for strategically deploying appropriate sources of SCN-resistance for managing SCN.
Publications
- No publications reported this period
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Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: (1) Impact of cultivar resistance on virulence phenotypes of the soybean cyst nematode: Field plot experiment was established in Waseca in 2003 to determine the effect of different sequences of SCN-resistance sources (PI 887888, Peking, and PI 437654) on dynamics of SCN population densities and their virulence phenotypes (HG Types or races). (2) Evaluation of SCN-resistance of soybean germplasms: Seventeen soybean cultivars and accessions that were reported resistant to HG Type 1- (race 14) or/and HG Type 2- (race 1) were tested against 13 different nematode populations including race 1 (HG Type 2-), race 2 (HG Type 1.2-), race 3 (HG Type 0-), race 4 (HG Type 1.2.3-) and race 14 (HG Type 1-) collected from Minnesota. (3) Identification of SCN-resistance QTLs: The QTLs conferring resistance to an HG Type 2.5.7 population (race 1) of SCN were investigated by analyzing genomes and phenotypes of SCN-resistance of 184 soybean MN0095 x PI 567516C F2:3 families. A total of 1536 SNP markers were analyzed for their association with SCN-resistance QTLs. (4) Breeding: Several soybean lines carrying SCN-resistance different from the sources PI 88788 and Peking were used for breeding SCN-resistant cultivars. Crosses were made in 2009, 2010, and 2011. The progenies of the crosses will be evaluated for SCN resistance. PARTICIPANTS: Principal Investigator: Senyu Chen. Collaborators: James Orf, Nevin Young, Bruce Potter, P. Schaus. Graduate students: Lian Lian, Yong Bao, Fang Wang. Technicians: Cathy Johnson, Wayne Gottschalk. TARGET AUDIENCES: Nematologists, plant pathologists, soybean breeders, agricultural professionals, extension educators, and soybean growers PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
(1) Impact of cultivar resistance on virulence phenotypes of the soybean cyst nematode: Among the three SCN-resistant cultivars, the cultivar carrying PI 88788 resistance supported highest SCN egg population density, the cultivar carrying Peking resistance intermediate, and cultivar carrying PI 437654 resistance supported lowest SCN egg population density. Based on the data of HG Type analysis of the populations collected in 2007, 2008, 2009, and 2010 from the plots in this experiment, it appeared that SCN populations selected by cultivar with PI 88788 source of resistance can only overcome the resistance of PI 88788 not the other two, and Peking-selected SCN populations can overcome the resistance in Peking, not the other two. PI 437654 can select SCN populations to overcome both sources of resistance PI 88788 and Peking. (2) Evaluation of SCN-resistance of soybean germplasms: Most of the lines tested had high or moderate resistance to race 1, race 2 and race 3 populations and can serve as an alternative resistance sources to PI 88788. However, most of the lines were susceptible to race 4 and the two race 14 nematode populations tested. Only PI 633736 has a high level of resistance to all the nematode populations used. PI 417091, PI 404166, PI 567516C, PI 629013 have moderate or high resistance to race 4 and at least one of the two race 14 populations. (3) Identification of SCN-resistance QTLs: Four SCN-resistance QTLs were determined including two significant QTLs and two suggestive QTLs. The two significant QTLs were detected on chromosome 10 and chromosome 19 and the two suggestive QTLs were detected on chromosome 8 and chromosome 18. The QTL located on chromosome 10 was recently reported by another group, and the QTL on chromosome 19 has not been reported previously. Haplotype analysis of rhg1 and Rhg4 genes for the 17 resistant soybean germplasms revealed that PI 567516C and Peking share the same rhg1 allele. Markers closest to rhg1 and the QTL on chromosome 10 might be considered for use in marker assisted selection. This study will provide useful information for strategically deploying appropriate sources of SCN-resistance for managing SCN.
Publications
- Zheng, J. W., and S. Y. Chen. 2011. Estimation of virulence type and level of soybean cyst nematode field populations in response to resistant cultivars. Journal of Entomology and Nematology 3:37-43.
- Lian, L., F. Wang, R. Denny, P. Schaus, N. Young, J. Orf, and S. Chen. 2011. Identifying novel sources of resistance to the soybean cyst nematod. Abstracts of 1st Annual National Association of Plant Breeders Meeting. Http://www.plantbreeding.org/napb/Meetings/2011/Abstracts11e05dsSJ.pd : 28.
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: 1. Impact of rotation of cultivars with different sources of resistance on virulence phenotypes of the soybean cyst nematode: Two field sites were established in Waseca and Lamberton, in 2003 and 2008, respectively, to determine the effect of different sequences of SCN-resistance sources (PI 887888, Peking, and PI437654) on dynamics of SCN population densities and their virulence phenotypes (races or HG Types). Waseca site was infested with initial population of SCN race 3, and the main aim of the experiment at this site was to determine how the cultivar sequences influence the changes of the SCN population from avirulent to virulent type on cultivars carrying certain resistance genes. At Lamberton site, the initial SCN population was race 1 that can overcome PI88788 resistance, and the main aim of the experiment at this site was to determine whether any cultivar sequence can change the population from virulent type to avirulent type or change to other HG Types so that the PI88788-source cultivars can be used. 2. Several soybean lines carrying SCN-resistance different from the sources PI 88788 and Peking were used for breeding SCN-resistant cultivars. Crosses were made in 2009 and 2010 and the progenies of the crosses will be evaluated for SCN resistance. PARTICIPANTS: Principal Investigators: Senyu Chen, James Orf, Bruce Potter; Research Scientists: P. Schaus. Research associate: Yong Bao. Technicians: Cathy Johnson, Wayne Gottschalk. TARGET AUDIENCES: Nematologists, plant pathologists, agricultural professionals, extension educators, and soybean growers PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
SCN population densities differed following different sequence of soybean cultivars, and susceptible soybean resulted in higher egg population density than resistant cultivars. Among the three SCN-resistant cultivars, the cultivar carrying PI88788 resistance supported highest SCN egg population density, the cultivar carrying Peking resistance intermediate, and cultivar carrying PI437654 resistance supported lowest SCN egg population density. Based on the data of HG Type analysis of the populations collected in 2007, 2008, and 2009, SCN populations selected by cultivar with PI 88788 source of resistance could overcome the resistance of PI 88788, and Peking-selected SCN population can overcome the resistance in Peking, not each other. In contrast, PI 437654 selected SCN population that could overcome both Peking and PI 88788 sources of resistance, although the population still had low reproduction potential on PI 437654. This study will provide useful information for strategically deploy appropriate sources of SCN-resistance for managing the nematode in Minnesota.
Publications
- No publications reported this period
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