Progress 10/01/13 to 09/30/14
Outputs
Target Audience: Soybean breeders, genetics researchers, soybean farmers Changes/Problems: A valued employee retired after more than a quarter-century of exceptional service. She will be very difficult to replace because of limited funding and her excellent knowledge, skills, and abilities that greatly helped to achieve project accomplishments. What opportunities for training and professional development has the project provided? Two Ph.D., two M.S., one pre-baccalaureate student, along with three research associates have received professional laboratory and field research training, including hands on experiences in plant breeding population development, progeny line selection, DNA isolation and analysis, gas chromatography, near infra-red reflectance, field trial management, field trial testing, phenotypic data acquisition, statistical analyses, data summarization, reporting, and decision making. How have the results been disseminated to communities of interest? Through state variety testing reports, refereed scientific journals, deposit of data to Soybase, presentations at professional society meetings, presentation at the 2014 Milan No-Till Field Day, and presentations and discussions at collaborator and other stakeholder meetings. What do you plan to do during the next reporting period to accomplish the goals? We plan to select among recently harvested single plants and advanced lines using single nucleotide polymorphisms (SNPs) for seed protein, oleic acid, and linolenic acid. We will make new cross combinations that bring together high seed yield and complementary assemblages of parental alleles for protein or oil quality and resistance to soybean cyst nematode (SCN), southern stem canker, frogeye leaf spot, or other prominent soybean diseases common in the region.
Impacts
What was accomplished under these goals?
We produced approximately 780,000 pounds of pure line seeds of our new conventional soybean variety ‘Ellis’ in 2014. Collaborators in the USDA Southern Uniform Soybean Tests adopted Ellis to become the new high yield check variety replacing ‘5002T’ in the maturity group IV-Late and maturity group V tests, where it will serve as a benchmark of success that other breeders will strive to match or beat. Ellis was tested in multiple locations of the 2014 Arkansas State Variety Test where it produced an extraordinary 73.1 bu/a statewide average yield. That yield (representing 8 bushels per acre above the test mean) ranked Ellis as #1 among 74 entries, and showed Ellis to be the highest yielding soybean of any maturity group or trait technology. It is notable that the Arkansas State Variety Test consists primarily of marketed commercial soybean varieties with technology traits – the test includes the very best industry soybeans currently available for sale to farmers. Ellis is an excellent example of how a Land Grant University can develop a strong, competitive variety and use the process as training program for the next generation of plant breeders. We collaborate with UniSouth Genetics, Inc. (USG) in multi-state field trials. Ellis soybean ranked #1 for yield in the 2014 USG trial where it produced an exceptional 71.6 bushels per acre yield (representing 8.7 bushels per acre above the test mean), averaged across all states (Arkansas, Missouri, and Tennessee). These accomplishment documents the outstanding field productivity of Ellis soybean. We developed new soybean lines with improved protein and oil quality this year. Many of them are able to produce 48% protein in the soymeal. Some of our new high protein lines can produce ultra-high protein (>50% protein) meal. In 2014 we chose hundreds of single plant selections for progeny rows that have >80% oleic acid in the lipid profile. That extraordinary level of monounsaturated fatty acid will eliminate the need for hydrogen, thereby eliminating trans fats. The high oleic oil will also provide excellent oxidative stability for food and industrial oil products. We detected quantitative trait loci (QTL) governing soybean protein, and amino acids of the protein. We detected genomic regions for seed oil concentration, and for the fatty acids: palmitic, stearic, oleic, linoleic, and linolenic. Our 2014 publication (Smallwood et al., 2014) listed above is the first report of confirmed QTL governing soybean seed isoflavones: cqSeed genistein-001 and cqSeed genistein-002, cqSeed daidzein-001 and cqSeed daidzein-002, cqSeed glycitein-001, and cqSeed isoflavone-001. The information about these genetic regions controlling soybean isoflavones has been deposited to Soybase.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
1) Smallwood, Christopher J., Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, Perry B. Cregan, David L. Hyten, and Vincent R. Pantalone. 2014. Detection and Confirmation of Quantitative Trait Loci for Soybean Seed Isoflavones. Crop Sci. 54:595606. doi: 10.2135/cropsci2013.05.0340
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Abrams, Jeneen, Vince Pantalone, Catherine Nyinyi Hatcher, Ben Fallen, Arnold Saxton, Caula Beyl and Dean Kopsell. QTL for Genetic Improvement of Soybean Amino Acid Composition. ASA-CSSA-SSA International Meeting Abstracts. November 2-5, 2014. Long Beach, CA.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Abrams, Jeneen, Vincent Pantalone, Catherine Nyinyi Hatcher, Ben Fallen, Arnold Saxton, Caula Beyl, and Dean Kopsell. Using MAS to Identify Soybean Recombinant Inbred Lines with Increased Protein Content. ASA-CSSA-SSA International Meeting Abstracts. November 2-5, 2014. Long Beach, CA.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Smallwood, Chris, and Vince Pantalone. Using Genomic Selection for Soybean Yield Improvement. National Association of Plant Breeders Annual Meeting Abstracts. August 6-8.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Smallwood, Chris, Hem Bhandari, Perry Cregan, Ben Fallen, Jason Gillman, David Hyten, Arnold Saxton, Phillip Wadl, and Vincent Pantalone. Comparison of Phenotypic and Genomic Selection Methods for Soybean Fatty Acids. ASA-CSSA-SSA International Meeting Abstracts. November 2-5, 2014. Long Beach, CA.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Willette, Alison, Jeffrey Boehm Jr., Forbes R. Walker, Hem Bhandari, Dean Kopsell, Kristin Bilyeu and Vincent R. Pantalone. Multi-Location Field Testing New Environmentally Friendly, High Yielding, Low Phytate Soybean Lines Developed Via Molecular Breeding. ASA-CSSA-SSA International Meeting Abstracts. November 2-5, 2014. Long Beach, CA.
|
Progress 01/01/13 to 09/30/13
Outputs
Target Audience: Soybean breeders, genetics researchers, soybean farmers Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Two Ph.D., one M.S., and two pre-baccalaureate students, along with three research associates have received professional laboratory and field research training, including hands on experiences in plant breeding population development, progeny line selection, field trial testing, data acquisition, statistical analyses, data summarization, reporting, and decision making. How have the results been disseminated to communities of interest? Through refereed scientific journals, deposit of data to Soybase, presentations at professional society meetings, and presentations and discussions at collaborator and other stakeholder meetings. What do you plan to do during the next reporting period to accomplish the goals? We plan to use classical and molecular breeding techniques for development and field testing of new genetic materials. We plan to select among newly developed advanced lines; select among new progeny rows, and make new cross combinations that bring together high seed yield and complementary assemblages of parental alleles for protein or oil quality and resistance to soybean cyst nematode (SCN), southern stem canker, frogeye leaf spot, and other prominent soybean diseases common in the region.
Impacts
What was accomplished under these goals?
We developed and released by the Tennessee Agricultural Experiment Station in 2013 the high yielding conventional soybean variety 'Ellis'. In prior testing, this new soybean variety ranked #1 for seed yield in the Tennessee State Variety Test where it produced 8 bushels per acre above the test average, and was also the highest yielding entry in the USDA Southern Uniform Test for its maturity group. We developed new soybean lines with improved protein and oil quality. Many new lines able to produce high protein soymeal are now in the plant breeding pipeline for field testing and selection. We detected quantitative trait loci (QTL) governing soybean amino acids, and principal component analyses related genomic data to amino acid pathway syntheses. We secured approval by the Soybean Genetics Committee for our molecular marker discoveries of confirmed QTL governing soybean seed isoflavones: cqSeed genistein-001 and cqSeed genistein-002, cqSeed daidzein-001 and cqSeed daidzein-002, cqSeed glycitein-001, and cqSeed isoflavone-001.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Fallen, B. D., C. N. Hatcher, F. L. Allen, D. A. Kopsell, A. M. Saxton, P. Chen, S. K. Kantartzi, P. B. Cregan, D. L. Hyten, and V. R. Pantalone. 2013. Soybean Seed Amino Acid Content QTL Detected Using the Universal Soy Linkage Panel 1.0 with 1,536 SNPs. Journal of Plant Genome Sciences, 1 (3): 6879.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Lin, J., M. Mazarei, N. Zhao, J. J. Zhu, X. Zhang, W. Liu, V. R. Pantalone, P. Arelli, C. N. Stewart, Jr., and F. Chen. 2013. Overexpression of a soybean salicyclic acid methlyltransferase confers resistance to soybean cyst nematode. Plant Biotechnology Journal, 11: 1135-1145.
- Type:
Other
Status:
Published
Year Published:
2013
Citation:
Cober, E. R., S. R. Cianzio, V. R. Pantalone, and I. Rajcan. 2013. Soybean. Handbook of Plant Breeding: Oil Crops, Science Press, China Science Publishing, Chinese Academy of Agricultural Sciences, Wuhan, Hubei, China.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
Arelli, P., V. R. Pantalone, J. G. Shannon, A. Mengistu, and L. D. Young. 2013. Marker assisted selection: a strategy for late generation testing to improve cyst nematode resistance for sustainable soybean production. World Soybean Research Conference IX, WSRC IX, South Africa, 17 to 22 February 2013.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
Boehm, J., V. R. Pantalone, F. R. Walker, H. Bhandari, D. A. Kopsell, and K. Bilyeu. 2013. Molecular Marker Assisted Backcross Development and Evaluation of An Environmentally Friendly, Commercially Acceptable Low Seed Phytate Soybean. Proceedings ASA CSSA SSSA Annual Meeting, Tampa, FL. November 3-6, 2013.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
Hussein, A., W. T. Schapaugh Jr., K. Rainey, V. R. Pantalone, G. Shannon, J. Klein, T. E. Carter Jr., A. J. Cardinal, E. R. Shipe, A. M. Gillen, P. Chen, H. R. Boerma, Z. Li, and D. B. Weaver. 2013. Genetic Gain for Seed Yield in Southern Soybean Cultivars. Proceedings ASA CSSA SSSA Annual Meeting, Tampa, FL. November 3-6, 2013.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
Smallwood, C., and V. R. Pantalone. 2013. Creation of a Training Set for Soybean Fatty Acid Improvement through Genomic Selection. Proceedings ASA CSSA SSSA Annual Meeting, Tampa, FL. November 3-6, 2013.
|
Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: Our breeding program has identified high yielding soybean lines from our 2012 yield trial data. We have moved forward with 13 of our advanced Conventional lines and 10 of our glyphosate resistant lines for cooperative testing with the USDA Southern Uniform Tests across a broad number of locations in the southern states for 2013. We have also entered 9 conventional and 8 glyphosate resistant lines into the 2013 Tennessee State Variety Tests where they will be grown in concert with commercial varieties and be evaluated as marketable prospects. Low linolenic soybean oil is a desirable trait associated with oil stability and can be considered an alternative to hydrogenation. Yield data from 2012 field plots alongside molecular marker data and gas chromatography analysis have identified 7 low linolenic lines with competitive yield to check cultivars. We have entered these into the 2013 Quality traits test. We have identified 12 individuals at F3 and F4 generations that combine the high oleic trait >85% with the low linolenic trait <3%. These generations will be advanced into F4 and F5 generation rows in 2013 with selected rows available for yield trials in 2014. We have made crosses and are working with populations that will produce improved low linolenic and higher oleic lines, along with improved protein lines to create new lines that can produce a 48% protein soybean meal. A major goal of our meal project was to develop an environmentally and agronomically superior soybean variety with low seed phytate for improved poultry and swine feed. Towards that aim, we have now developed two promising BC5 derivatives of the high yielding Tennessee cultivar 5601T and our low phytate line TN09-239. These lines, 56CX-1273 and 56CX-1283 are high yielding, determinate, and display high seed germination. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
Breeding for high yield and targeting producer needs for valuable traits remains a top priority for our program. During 2012, a total of 6,366 soybean breeding test plots were harvested from the Research and Education Centers at Milan, Jackson, Springfield, and Knoxville, TN and illustrates the program's dedication to applied variety development. Yield testing is one of the most important research activities that we do for both our conventional and our glyphosate resistant program that allows us to develop and bring new soybean varieties to producers in the region. In 2012 we grew thousands of conventional and glyphosate resistant population rows and moved selections forward for yield testing. Further impacts are seen through the projects of our graduate students. One Ph.D. student graduated in 2012 with research involving nearly 1,000 recombinant inbred lines of Essex x Williams 82. This project was analyzed by USDA-ARS collaborators (Dr. Perry Cregan and staff, Beltsville, MD) with >50,000 SNP markers to create an enhanced density SNP map that will update the current USDA soybean consensus linkage map. We currently have two Ph.D. students continuing with this project. Specifically they will be searching to identify markers for use in seed yield, oil and fatty acid, and protein and amino acid improvements.
Publications
- Fallen, B.D., K.M. Rainey, C.E. Sams, D.A. Kopsell and V.R. Pantalone. 2012. Evaluation of Agronomic and Seed Characteristics in Mid-Oleic Soybean Lines in the South-Eastern United States. J. Am. Oil Chem. Soc. 89:1333-1343. Fallen, B.D. 2012. Detection of Soybean Amino Acid QTLs and Seed Yield QTLs Using Selective Genotyping. PhD Dissertation. University of Tennessee.
- Fallen, Ben, Arnold Saxton, Fred Allen, Dean Kopsell and Vincent Pantalone. 2012. Selective Genotyping for Marker Assisted Selection Strategies for Soybean Yield Improvement. ASA-CSSA-SSSA International Annual Meeting. October, 2012. Cincinnati, OH. Smallwood, Christopher, Dean Kopsell, Carl Sams, Dennis West, and Vincent Pantalone. 2012. Validation and Detection of Quantitative Trait Loci for Soybean Isoflavones. ASA-CSSA-SSA International Meeting Abstracts. Oct. 21-24, 2012. Cincinatti, Ohio.
- Smallwood, Christopher, Dennis West, Carl Sams, Dean Kopsell, and Vincent Pantalone. 2012. Validation and Detection of QTL for Soybean Isoflavones. AOCS Annual Meeting Abstracts. April 29-May 2, 2012. Long Beach, California.
|
Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: Our 2011 laboratory and field trials identified soybean candidate lines in the breeding program with high yield and improved seed quality. For example, soybean line TN05-5018 was the highest yielding conventional entry grown over 22 locations of the USDA Southern Uniform Test; that same line was also the top yielding entry in the 2011 Tennessee State Variety Test. Several new lines developed by our program exceeded the average yield of commercial cultivars in the 2011 Tennessee State Variety Test. A notable example is the glyphosate herbicide resistant cultivar USG 75T40 developed by our program, which ranked #1 for seed yield, averaged over all locations in Tennessee in 2011 as well as in the two and three year averages. Its excellent resistance to soybean cyst nematode (SCN) Race 2 and strong field tolerance to sudden death syndrome (SDS) further document the value of utilization of exotic material from the USDA Soybean Germplasm Collection for improving seed yield and pest resistance. Its pedigree includes the parental line LG98-1445, which is the registered germplasm line GP-318 (Dr. Randy Nelson, USDA-ARS) which contains two exotic pedigree sources (PI 227333 and PI 91730-1) that collectively contributed less than 10% of the genes to U.S. soybean cultivars. Breeder elite, intermediate and preliminary field tests grown at Tennessee Research and Education Centers in 2011 identified promising candidates for broad scale field testing. For example, for 2012 we have entered 27 lines to the USDA Southern Uniform Testing Program and 13 advanced lines to the 2012 Tennessee State Variety Test. Single nucleotide polymorphism (SNP) marker technology continues to enable us to select individual single plants and lines for the two low phytate confirmed QTL qPha-001 and cqPha-002 to improve soybean meal. We have used additional SNP markers in 2011 to select for improved protein concentration and low (<3%) linolenic acid concentration for oil quality improvement. We planted thousands of conventional or glyphosate resistant plant rows and single plants in 2011. We accomplished new cross combinations targeting yield improvement; genetic diversity; SCN resistance; Asian soybean rust resistance, increased protein content; increased oleic acid for improved oil oxidative stability; 1% linolenic acid for zero trans-fat oil; 3% linolenic acid for reduced trans-fat oil; and low phytate for improved poultry and swine nutrition and reduced phosphorous pollution to the environment. We are developing availability options for conventional, edamame, or glyphosate resistant lines for soybean producers. We adopted a new targeted goal of developing lines able to produce 48% meal. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
Breeding for high yield and targeting producer needs remains a top priority for our program. Our cultivar USG 75T40 produced 5 Bu/A greater yield than the average of commercial cultivars in the 2011 Tennessee State Variety Test for its maturity class. Its superior resistance to SCN Race 2 is valuable for the Mid-South soybean production region. Conventional and glyphosate resistant cultivars developed by our program were commercially produced over >75,000 acres in the past three years, with yield advantage x favorable commodity prices translating to an impact of approximately $3,750,000 in additional revenue over the past three years to farmers (or $1.25 million annually), directly by our soybean breeding program. Replicated field trials conducted at four Tennessee Locations: the East Tennessee Research and Education Center (ETREC), the Highland Rim Research and Education Center (HRREC), the Research and Education Center at Milan (RECMLN) and the West Tennessee Research and Education Center (WTREC) continue to prove valuable in development of elite soybean lines that are productive over a broad geographical region of the south, as evidenced by TN05-5018, the top yielding entry evaluated over 22 locations in the USDA Southern Uniform Test. The value of our testing program is further illustrated by additional new lines that exceed yields of commercial cultivars or checks in the 2011 Tennessee State Variety Test, as well as in Breeder Elite, Intermediate, and Preliminary tests. 2011 was a record year of field plot research with a total of 9,328 yield plots by our breeding program. Further impacts are seen through the projects of our graduate students. One Ph.D. student graduated in 2011, with gainful employment as soybean breeder for a major seed company. Two M.S. students who are about to graduate have already accepted employment offers with a major seed company. Another current Ph.D. student project involving nearly 1,000 recombinant inbred lines of Essex x Williams 82, working collaboratively with USDA-ARS collaborators (Dr. Perry Cregan and staff, USDA-ARS) has mapped polymorphic markers from >50,000 SNP assay. The new map will be further utilized to enhance the density and update the current USDA soybean consensus linkage map.
Publications
- No publications reported this period
|
Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: Based on 2010 field trials, we have some excellent high yielding soybean candidate lines in the breeding program pipeline. We have moved forward 18 of our advanced conventional lines and 13 of our advanced next generation glyphosate herbicide resistant lines for cooperative testing with the USDA Southern Uniform Tests across a broad number of locations among many southern states. Sixteen of these lines (11 glyphosate resistant and 5 conventional) have been entered into the 2011 Tennessee State Variety Tests where they will be grown alongside commercial cultivars to gauge their commercial potential for soybean producers. Using single nucleotide polymorphism (SNP) marker technology, with new SNPs constructed directly from soybean (Glycine max) DNA sequence information, we can now select individual single plant progenies with 100% accuracy for each of the two low phytate genes, corresponding to our confirmed simple sequence repeat (SSR) molecular markers cqPha-001 and cqPha-002, which are located on soybean chromosome numbers 3 and 19. We have used SNP markers this year to confirm that our new low phytate soybean line TN09-239 is double homozygous recessive for the two alleles that express low phytate concentration in soybean seeds. Data analyzed from the 2010 Quality Traits Test showed that our low phytate line TN09-239 averaged 93% the yield of 5601T (its recurrent parent cultivar and 107% the yield of all 27 test entries. Data from the 2010 USDA Southern Uniform Preliminary Test showed that our new low phytate line was equivalent to its recurrent parent for seed protein concentration (405 g Kg-1 for TN09-239 vs. 403 g Kg-1 for 5601T), seed oil concentration (202 g Kg-1 for TN09-239 vs. 201 g Kg-1 for 5601T), reaction to soybean cyst nematode HG types 1.2.5.7 and 5.7, stem canker resistance score, flower, pubescence, and pod color, and days to plant maturity. However the seed yield of the low phytate line TN09-239 (2,714 Kg ha-1) was 85% the yield of 5601T (3,185 Kg ha-1) and was 91% of the yield of all 47 test entries, which included four commercial cultivars. We are currently attempting to break a linkage between one of the low phytate alleles (Pha-002) and a gene that determines the indeterminate growth habit inherited from the donor parent CX-1834-1-2. Determinate growth habit is desirable for our new line to improve lodging resistance and increase seed yield. Currently 60 single BC5F2 plants which exhibited determinate growth habit and were found to be double homozygotes for the low phytate trait are being produced in our winter greenhouse. We planted thousands of conventional or glyphosate resistant plant rows and single plants in 2010. We accomplished new cross combinations targeting yield improvement; genetic diversity; SCN resistance; increased protein content; increased oleic acid for improved oil oxidative stability; 1% linolenic acid for zero trans-fat oil; 3% linolenic acid for reduced trans-fat oil; and low phytate for improved poultry and swine nutrition and reduced phosphorous pollution to the environment. We are developing availability options for conventional, edamame, or glyphosate resistant lines for soybean producers. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
In 2010, we released a new Roundup Ready(R) cultivar developed by our program, and marketed by UniSouth Genetics (Dickson, TN) as USG 75T40. This line is noteworthy because it is resistant to SCN Race 2 (HG Type 1.2.5.7), and moderately resistant to SCN Race 14 (HG type 1.3.5.6.7). This new cultivar is also notable in its exceptionally strong field tolerance to sudden death syndrome (SDS), caused by Fusarium solani. The SDS disease index (DX) score was 2.8, which was an order of magnitude lower than the average DX of all commercial cultivars (29.5) in the 2009 Tennessee State Cultivar Test and our cultivar had the lowest SDS DX rating among the highest yielding cultivars. Breeding for high yield and targeting producer needs remains a top priority for our program. 2010 launched our first replicated yield trials of next generation glyphosate resistance technology when we evaluated over 150 new Tennessee lines from SCN resistance genetic backgrounds. These materials were harvested from replicated field test plots during 2010 at four Tennessee Locations: the East Tennessee Research and Education Center (ETREC), the Highland Rim Research and Education Center (HRREC), the Research and Education Center at Milan (RECMLN) and the West Tennessee Research and Education Center (WTREC). In the early MG IV test two of our new lines were the top yielders, exceeding all commercial checks. In the late MG IV test, one of our new lines was the highest yielder and eight others exceeded the mean of the commercial checks. In the MG V test, two of our new lines were the top yielders, exceeding all commercial checks. During 2010, a total of 7,685 soybean breeding yield test plots were harvested from the Research and Education Centers at Milan, Jackson, Springfield, and Knoxville, TN. This represents a major investment in resources by the Tennessee Agricultural Experiment Station, and strongly illustrates the Soybean Breeding & Genetics Program's dedication to applied cultivar development. Yield testing is one of the most important research activities that we do to develop and bring new soybean cultivars to producers in the region. Impacts are seen through the projects of our graduate students. We have 3 MS and 2 PhD students involved in breeding and genetic projects to improved soybean quality and production. For example, working with USDA-ARS collaborators we have mapped recombinant inbred lines (RIL) using the 1536 soybean universal linkage panel, and we now have a very large RIL population developed with 17,000 polymorphic markers to determine quantitative trait loci for marker assisted selection in soybean.
Publications
- No publications reported this period
|
Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: In 2009 our University of Tennessee (UT) AgResearch Soybean Breeding & Genetics Program developed a series of high yielding new conventional (non-GMO) lines with excellent resistance to multiple races of soybean cyst nematode (SCN). Five of those lines have been entered to the 2010 Tennessee State Variety Test to gauge commercial potential. Based on 2009 field trials, we have moved forward 24 of our advanced conventional lines for cooperative testing with the USDA Southern Uniform Tests across a broad number of locations among many southern states for 2010. This includes one entry with low phytate concentration, a trait that will enhance poultry and swine nutrition, and reduce phosphorous pollution to our freshwater streams, rivers, and lakes. We have developed hundreds of new lines with next generation glyphosate resistance. Many of these lines also have SCN resistance. Eleven of these lines are being planned for advanced 2010 tests in collaboration with UniSouth Genetics, Inc. (Nashville, TN) in replicated field evaluations in Tennessee, Arkansas, and Missouri. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
We have been working on developing a new high yielding maturity Group V Roundup Ready(R) cultivar that is earlier in maturity than USG Allen. Line TN06-140RR produced the 2nd highest yield in the 2009 Tennessee State Variety Test of early group V soybean cultivars, at 63 Bu/A, which was 9 Bu/A above the average of 42 commercial cultivars in that test. We produced over 450 bushels of Breeder Seed at Milan, TN this past year, which averaged over 70 Bu/A at that Research and Education Center. We anticipate drafting recommendations for UT AgResearch to release TN06-140RR as a new early Group V Roundup Ready (R) cultivar that will enhance farm profitability for the mid-south region. One of our high yielding early maturity group IV conventional lines, TN05-3027 is unusual in that it comprises 37.5% exotic pedigree, where its genetic diversity is based upon three plant introductions from the USDA soybean germplasm collection. That line arose from a heterogeneous population developed by the USDA-ARS, with localized reselections conducted in Tennessee. TN05-3027 is being shared as a crossing parent among breeders with the 2010 USDA Southern Uniform and Northern Uniform Tests, and with the United Soybean Board Diversity Tests. The USDA-ARS and University of Tennessee AgResearch collaboratively developed and released soybean germplasm line SB-01. This new line contains the two publicly available novel desaturase alleles designated as the gene mutations FAD3A and FAD3C that together produce less than 3% linolenic acid. Detection of these novel alleles enabled selection based on perfect molecular markers of soybean microsomal omega-3 fatty acid desaturase genes. As a consequence, breeders can utilize SB-01 germplasm along with the perfect molecular markers to accelerate trait introgression to elite lines to serve the low trans fat food products industry. By providing technology for soybean breeders to improve the domestic supply of low trans fat vegetable oil, we will impact goals toward improving consumer health.
Publications
- No publications reported this period
|
|