Source: KENTUCKY STATE UNIVERSITY submitted to NRP
ADVANCEMENT OF ALTERNATIVE AQUACULTURE SPECIES FOR KENTUCKY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0220006
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2009
Project End Date
Sep 30, 2013
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
KENTUCKY STATE UNIVERSITY
(N/A)
FRANKFORT,KY 40601
Performing Department
Aquaculture
Non Technical Summary
Channel catfish remains the nation's largest aquaculture industry. However, there has been a negative trend in production in recent years. Catfish growers in the U.S. had sales of 410 million dollars during 2008, down 10 percent from 455 million dollars the previous year (NASS 2008). The industry has been negatively impacted over the past few years by a combination of factors. There has been an influx of low priced imports competing with catfish in the marketplace. Also, high feed prices, brought on by increase in grain prices, have negatively impacted profitability. This has resulted in a strong need for the development of alternative aquaculture species, especially those which can be marketed in product forms such as live or fresh, which will be hard for foreign producers to address. Also, there will be a continuing need for reducing feed costs for both alternative and traditional aquaculture species. It is expected that these results will support further development of the expanding the production of each of these species in Kentucky and the US. These improvements will allow per unit production, feed conversion efficiency, and average harvest weights to be maximized, and new markets to be addressed, increasing commercial viability.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3023710106020%
3023714106020%
3023719106020%
3033722106020%
3063722106020%
Goals / Objectives
A project evaluating alternative aquaculture species for suitability to production and marketing conditions in the region was conducted several years ago at Kentucky State University (KSU). Evaluations were based on several criteria, including: 1) production technologies which are adaptable to a large number of producers, 2) good growth under regional conditions, 3) markets allowing significant production increases without requiring large-scale processing capabilities, and 4) ability to generate per acre net returns comparable to tobacco. The purpose of the project proposed here is to address the most serious constraints to production expansion for four species which show significant potential in Kentucky. These species are the largemouth bass, channel catfish, freshwater prawn, and tilapia. Objectives include evaluation of the use of artemia replacement products in the larvae culture of largemouth bass. Determination of the toxicity of copper sulfate to largemouth bass of different sizes and histories (i.e. naive vs. pre-exposed) and under different environmental conditions. Evaluation of the impact of dietary taurine supplementation for largemouth bass. Evaluation of new high protein forms of distillers grains in channel catfish diets. Comparison of the production characteristics of hybrids produced from the crosses of three genetic strains of freshwater prawns. Examination of the role of light intensity and/or wavelength in the larviculture of the freshwater prawn. The research will be conducted in both laboratory and field settings between fall 2009 and fall 2013. It is expected that these results will support further development of the expanding the production of each of these species in Kentucky and the US. These improvements will allow per unit production, feed conversion efficiency, and average harvest weights to be maximized, and new markets to be addressed, increasing commercial viability.
Project Methods
Six of the seven objectives will be tank experiments. These will be conducted in replicated trails. Previous experience indicates that 4-8 replicates will be sufficient for the statical power needed to identify real and biologically significant differences. One trial will be conducted in ponds. In that system we will be constrained to 3-4 replicates per treatment. For most studies comparisons will be made using Analysis of Variance (ANOVA). Treatments will be compared in terms of survival, average weight, total weight produced, and body composition. If ANOVA indicates significant treatment effects the Least Significant Difference test (LSD) will be used to determine differences among treatment means. All percentage and ratio data will be transformed to arc sin values prior to analysis. Researchers submit Quarterly Reports on each project to the Research Director and Associate Research Director. This gives the administrators an opportunity to assess research productivity and impact. These reports are also assembled into a comprehensive Annual Report so that the different projects can be evaluated over a longer timeframe. Another opportunity for assessment is the development of CRIS Reports. These are available to any interested party. The sections which ask the writer to address "Change in Knowledge" and "Change in Action" documents how the research has changed and improved during its conduct. Opportunities for assessment and the use of results for improvement also include a review of the research efforts by the "Aquaculture as the Program of Distinction - External Advisory Committee". The committee is made of stakeholders, related support businesses, and research and extension professionals from other universities. They assess research productivity over a 2-3 year or full project time period. The committee can evaluate not only program productivity and impact but also program needs as well as make suggestions on research direction at both the project and program level.

Progress 10/01/09 to 09/30/13

Outputs
Target Audience: Farmers andExtension personnel involved in production aquaculture in Kentucky were the target audience. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? All research projects involved mentoring ofgraduate and undergraduate students who greatly benefit from the opportunity of hands-on involvement in practical and applied agricultural research and development. How have the results been disseminated to communities of interest? As part of the objectives, researchers have worked closely with industry and students in the training and dissemination of results. Researchers collaborate closely with Kentucky Aquaculture Association and the Kentucky Department of Agriculture to ensure research based information is disseminated in a timely manner to constituents through workshops and training events. KSU researchers have an excellent relationship with the production industry and are often directly involved in on-farm validation and scale-up of operations. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? This research effort has resulted in improved aquaculture production technologies and practices in Kentucky and the region. Development of technologies which have allowed intensification of largemouth bass larviculture have decreased production costs and resulted in improved reliability of seedstock production. As part of this research, researchers worked closely with industry to develop commercial scale production of largemouth bass juveniles out of season which has decreased our reliance on out of state suppliers and improved overall production efficiency. The determination of copper sulfate tolerance of largemouth bass at different temperatures has greatly improved our growers ability to prevent disease outbreaks in largemouth during harvest and transport procedures. This is important because largemouth bass are particularly sensitive to many warm and cold water aquatic disease problems and nearly all production costs have been incurred at this time. Taurine determination has assisted in providing fish nutritionists with the necessary information to develop a species specific diet for largemouth bass. With this and data from previous research supported by NIFA, KSU researchers are now working with a local pet food manufacturer to produce species specific diets for largemouth bass in Kentucky, reducing feed costs to farmers by greater than 20%. We determined that high protein distillers dried grains (DDG)are well accepted and utilized by channel catfish. This is especially important as channel catfish is the largest segment of aquaculture in the US as well as in KY, and DDG from ethanol distillation is now widely available due to recent increases in ethanol production. There are also new technologies for ethanol manufacturing which produce higher protein co-products which has increased the production of DDG. Research on freshwater prawn larviculture and the evaluation of different genetic strains has resulted in improved production technologies for one of the most economically important crustacean species in the world. Producers in Kentucky and globally have benefitted from KSU's research efforts in maximizing production technologies for the freshwater prawn. This research has supported the continued expansion of aquaculture production in Kentucky and the US through improved production efficiency and technological development.

Publications

  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Tidwell, J.H., Coyle, S.D., Bright, L.A., Shultz, C., Kupchinsky, Z.A., Schwartz, P. and C. Upstrom. In Press. Production comparison of all-male and mixed-sex freshwater prawns Macrobrachium rosenbergii in ponds. Journal of the World Aquaculture Society.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Blair, D., Oliver, L., Kupchinsky, Z., Watts, C., Bright, L. A., Coyle, S. D. & Tidwell, J. H. 2013. Comparison of different light technologies for indoor aquaponics production of white-stemmed Pak Choi Brassica rapa var. chinensis. The 99th Kentucky Academy of Science Annual Meeting. Morehead State University, Morehead, Kentucky. November 9.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Coyle, S. and J. Tidwell. 2013. Hog-barn aquaculture using air lift pumps and a hybrid microbial system. Aquaculture America, Nashville, TN, February 21-25: #237.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Coyle, S., Tidwell, J. and L.A. Bright. 2013. Indoor biofloc systems in temperate regions. Aquaculture America, Nashville, TN, February 21-25: #235.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Coyle, S., Tidwell, J. and L.A. Bright. 2013. Largemouth bass production. Aquaculture America, Nashville, TN, February 21-25: #236.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Frederick, C., Coyle, S., Tidwell, J. and R. Durborow. 2013. The effects of taurine supplementation in largemouth bass Micropterus salmoides diets on growth performance and survival. Aquaculture America, Nashville, TN, February 21-25: Board 184.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Frederick, C.A., Coyle, S.D., Tidwell, J.H. and R.M. Durborow. 2013. The effects of taurine supplementation in largemouth bass Micropterus salmoides diets on growth performance and survival. Association of 1890 Research Directors, Inc., Jacksonville, FL, April 7-10: 6.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Kupchinsky, Z., Coyle, S.D., Bright, L.A., Shultz, C., Upstrom, C. and J.H. Tidwell. 2013. Production comparison of all-male and mixed-sex prawns Macrobrachium rosenbergii in ponds. Association of 1890 Research Directors, Inc., Jacksonville, FL, April 7-10: 8.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Kupchinsky, Z. A., Bright, L. A., Coyle, S. D. & Tidwell, J. H. 2013. Evaluation of heterotrophic algae meal as a protein source for channel catfish (Ictalurus punctatus) diets. The 99th Kentucky Academy of Science Annual Meeting. Morehead State University, Morehead, Kentucky. November 9.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Sandifer, P., DAbramo, L., Malecha, S. and J. Tidwell. 2013. Early history and pioneers of freshwater prawn aquaculture. Aquaculture America, Nashville, TN, February 21-25: #769.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Shultz, R.C., Kupchinsky, Z., Coyle, S.D., Bright, L.A. and J.H. Tidwell. 2013. Comparison of tilapia Oreochromis niloticus growth, water quality and microbial community structures in indoor biofloc system supplemented with either organic or inorganic carbon sources. Association of 1890 Research Directors, Inc., Jacksonville, FL, April 7-10: 329.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Tidwell, J.H. 2013. A review of challenges of inland aquaculture in temperate regions of the U.S. Sandifer, P., DAbramo, L., Malecha, S. and J. Tidwell. 2013. Early history and pioneers of freshwater prawn aquaculture. Aquaculture America, Nashville, TN, February 21-25: #354.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Tidwell, J., Coyle, S.D., Bright, L.A., Shultz, C., Kupchinsky, Z. and C. Upstrom. 2013. Production comparison of all-male and mixed-sex prawns Macrobrachium rosenbergii in ponds. Aquaculture America, Nashville, TN, February 21-25: #935.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Tidwell, J.H. and S.D. Coyle. 2013. Potential use of the African River Prawn Macrobrachium vollenhoveni as a biological control for schistosomiasis in West Africa. Association of 1890 Research Directors, Inc., Jacksonville, FL, April 7-10: 208.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Shultz, C. 2013. Aquaponics. 29th Annual Alltech International Symposium, Lexington, KY, May 21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Shultz, C. 2013. Commercial aquaponics experience in the Caribbean and considerations for temperate climate success. Aquaculture Canada 2013-Farming our Waters: Agrifoods Innovations, Guelph University, Guelph Ontario, Canada. June 2-5.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Tidwell, J.H. 2013. A review of challenges of inland aquaculture in temperate regions of the U.S. Aquaculture America, Nashville, TN, February 21-25.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2013 Citation: Coyle, S. D. 2013. Cage construction. Kentucky Aquaculture Association Annual Meeting, Kentucky State University, October 26.
  • Type: Other Status: Other Year Published: 2013 Citation: Coyle, S. 2013. Cage Culture. Kentucky State University Third Thursday, May 16.
  • Type: Other Status: Other Year Published: 2013 Citation: Coyle, S. D. 2013. Cage construction. Kentucky Aquaculture Association Annual Meeting, Kentucky State University, October 26. C
  • Type: Other Status: Other Year Published: 2013 Citation: Coyle, S. 2013. How to fillet fish. KSU-OSU Aquaculture Boot Camp Workshop. KSU/ARC August 9-10.
  • Type: Other Status: Other Year Published: 2013 Citation: Shultz, C. 2013. Aquaponics. Kentucky State University Third Thursday, May 16.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2013 Citation: Tidwell, J.H. 2013.Aquaponics. Kentucky Aquaculture Association Annual Meeting, Kentucky State University, October 26.
  • Type: Other Status: Other Year Published: 2013 Citation: Tidwell, J. H. 2013. An overview of aquaponics. Empowering Women Veterans: Business, Agriculture and Well-Being. Louisville, Kentucky, November 15.


Progress 01/01/12 to 12/31/12

Outputs
OUTPUTS: Three studies were conducted during the reporting period. The first addressed Objective 3, to evaluate the effect of supplementing largemouth bass diets with the amino acid taurine. This builds off of a long series of bass nutrition research at KSU. In other strict predators adding taurine to the diet has improved survival, growth, and liver health. After 10 weeks there was no difference in weights of fish fed the four diets. Objective 4. Following up on evaluation of distillers dried grains (DDGS) in catfish diets we evaluated a new algae meal product. Just as fuel ethanol production has increased the supply 0f DDGS, the increased production of algae for biofuel is producing increasing amounts of algae meal. This study evaluated diets containing 10%, 20%, 40%, and 40% algae meal with added lysine. After 8 weeks, fish fed the diets containing algae meal were larger than fish fed control diets. Objective 5 was a follow up on prawn production. Freshwater prawns have a complex social structure which affects the growth of certain sexual morphotypes which impacts production and especially size variation. By growing all-males it may be possible to reduce this negative interaction. All-males were grown in three ponds and mixed-sex in three ponds. After 105 days total weight harvested did not differ between treatments. However, the percentage of harvested animals achieving market and premium weights (>30, >40g) was significantly higher in the all-male prawns. PARTICIPANTS: Dr. James Tidwell is the principal investigator on this project and Mr. Shawn Coyle is the co-investigator. The information generated from this research was recently presented at as the World Aquaculture Society annual meeting and will be published in appropriate Jourals for dissemination to the greater aquaculture community. TARGET AUDIENCES: The target audience for this research are existing aquaculture producers, persons interested in aquaculture, agriculture extension agents and aquaculture research personnel interested in the areas of nutrition, husbandry and chemistry. This is applied research with immediate application to on-farm use addressing basic research questions also important to the aquaculture community such as digestive enzyme function of larval fish and importance of organic carbon on heterotrophic bacteria. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Objective 3: Based on this research it appears that the extra expense of taurine supplementation would not be justified in commercial diets. For Objective 4, these results were generated in cooperation with Eastern Kentucky University Center for Renewable Fuels Technology Center and Alltech Biotechnolgy Center. Alltech has already initiated a trial to follow up on these results and are working with KSU to evaluate commercialization of feed formulations. For Objective 6, economic viability of prawn production in the region should be improved based on these results.

Publications

  • Tidwell, J. H. 2012. Current status and prospects of farming the giant river prawn (Macrobrachium rosenbergii De Man 1879) in the USA. Aquaculture Research 43:1023-1028.
  • Skudlarek, N., Coyle, S., and Tidwell, J. 2013. Development of first-feeding protocols for indoor larviculture of largemouth bass (Micropterus salmoides). Journal of Applied Aquaculture 25: 9-23.


Progress 01/01/11 to 12/31/11

Outputs
OUTPUTS: OUTPUTS: 2011/01 TO 2011/12 Two studies were conducted during the reporting period. The first addressed Objective 1, to evaluate commercially available Artemia replacement products and marine larval diets for first feeding of largemouth bass larvae. Traditionally largemouth bass fingerlings are initially reared in nursery ponds on natural zooplankton then transferred into tanks for feed training. This research builds off prior research in which largemouth bass larvae were successfully fed Artemia nauplii for two weeks followed by a gradual transition to salmonid starter feeds. This line of research has the potential to eliminate the unpredictable nursery pond phase improving inventory control and production planning. It could also allow for out-of-season controlled indoor spawning as timing is no longer associated with pond zooplankton availability. Objective 7: We evaluated sucrose application rates of either 0% (C:N=9.2), 25% (C:N= 12.4) or 40% (C:N=15.4) of the daily feed rate with feed application rates being reduced proportionally. Alkalinity was maintained at 100 mg/L by daily sodium bicarbonate additions in all treatments; with the 0% sucrose treatment serving as the inorganic carbon treatment. After 10 weeks, there were no significant differences in average weight or survival of tilapia between treatments. FCR and average dissolved oxygen concentrations were significantly lower in the 40% sucrose treatment; possibly indicating a benefit to the reduced feed rates and a disadvantage of the increased sucrose application. It appears that either organic or inorganic carbon can be used to satisfy the carbon requirement of heterotrophic bacteria in zero water-exchange biofloc systems. These findings have been disseminated through workshops and directly to end-users via on-farm instruction. PARTICIPANTS: Dr. James Tidwell is the principal investigator on this project and Mr. Shawn Coyle is the co-investigator. The information generated from this research was intitially disseminated through the annual meeting of the Kentucky Aquaculture Association, a producer organization. Subsequently, site visits and on-farm training were provided to interested farmers who have readily adopted and are utilizing these technologies. The results of both of these studies have been immediately implemented by private producers in Kentucky. Mayer's fish farm of Bardstown, KY has constructed an indoor system for intensive larviculture of largemouth bass and has been moderately successful in the first year. In 2011, Mayer's fish farm produced approximately 10,000 feed trained largemouth bass completely indoors, an estimated value of $5,000. Phillip McCoun of Shelbyville has converted a swine farrowing barn to indoor heterotrophic tilapia production using inorganic carbon supplementation. The first crop is doing well and he is currently expanding the operation.The results from these trials will also be presented at professional meetings such as the World Aquaculture Society annual meeting and will be published in appropriate Jourals for dissemination to the greater aquaculture community. TARGET AUDIENCES: The target audience for this research are existing aquaculture producers, persons interested in aquaculture, agriculture extension agents and aquaculture research personnel interested in the areas of nutrition, husbandry and chemistry. This is applied research with immediate application to on-farm use addressing basic research questions also important to the aquaculture community such as digestive enzyme function of larval fish and importance of organic carbon on heterotrophic bacteria. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
OUTCOMES / IMPACTS: 2011/01 TO 2011/12 Objective 1: ArtemacTM (Bio-Marine) and BioVita (Bio-Oregon) were compared with de-capsulated Artemia cyst as transitional diets after either one or two weeks of Artemia feeding. Fish fed live Artemia nauplii for two weeks and then transitioned to decapsulated Artemia for one week were significantly larger and had better survivals than fry fed other combinations. Based on this work it appears that it may be necessary to feed live feeds initially as the enzymatic digestive system of first feeding largemouth bass fry is likely not sufficiently developed to utilize prepared diets. This trial was conducted under semi-commercial conditions at a private producer farm to facilitate technology transfer. Over 10,000 feed trained fingerlings were produced, six weeks earlier than using conventional methods. The second study addressed Objective 7. It investigated tilapia growth, and water quality and microbial community structures in indoor biofloc systems supplemented with either inorganic or organic carbon sources. In recent years, zero water-exchange biofloc systems have been experimentally developed for marine shrimp and tilapia production utilizing well-mixed suspensions of particulate matter and associated microbial communities. Often referred to as heterotrophic biofloc systems, these microbial communities are usually dominated by heterotrophic bacteria requiring carbon supplementation for efficient nitrogen removal. One of the most significant challenges to stability and efficiency of production in superintensive bio-floc-based systems relates to management of carbon and nitrogen inputs. Customarily organic carbon in the form of simple sugars are added with the feed ration to achieve a C:N of >15. A major disadvantage to this approach is the increase in BOD associated with sugar application, and the large volume of waste-solids generated. The results of both of these studies have been immediately implemented by private producers in Kentucky. Mayer's fish farm of Bardstown, KY has constructed an indoor system for intensive larviculture of largemouth bass and has been moderately successful in the first year. In 2011, Mayer's fish farm produced approximately 10,000 feed trained largemouth bass completely indoors, an estimated value of $5,000. Phillip McCoun of Shelbyville has converted a swine farrowing barn to indoor heterotrophic tilapia production using inorganic carbon supplementation. The first crop is doing well and he is currently expanding the operation.

Publications

  • Skudlarek, J. G., Coyle, S. D., Bright, L. A. and J. H. Tidwell. 2011. Effect of holding and packing conditions on hemolymph parameters of freshwater prawns, Macrobrachium rosenbergii, during simulated waterless transport. Journal of the World Aquaculture Society 42(5): 603-617.
  • PUBLISHED ABSTRACTS: Coyle, S., Moore, S., Schneider, K. and J. Tidwell. 2011. Acute toxicity of Diquat to juvenile freshwater prawn Macrobrachium rosenbergii. Aquaculture America, New Orleans, Louisiana, p. 96.
  • Coyle, S., Schneider, K. and J. Tidwell. 2011. Use of Artemac as a larval diet for the freshwater prawn Macrobrachium rosenbergii. Asian-Pacific Aquaculture & Giant Prawn, Kochin, India, p. 97.
  • Coyle, S., Schneider, K. and J. Tidwell. 2011. Use of Artemac as a larval diet for the freshwater prawn Macrobrachium rosenbergii. Aquaculture America, New Orleans, Louisiana, p. 96.
  • JOURNAL PUBLICATIONS: Coyle, S. D., Bright, L. A., Wood, D., Neal, R. and J. H. Tidwell. 2011. Performance of Pacific white shrimp Litopenaeus vannamei reared in zero-exchange tank systems exposed to different light sources and intensities. Journal of the World Aquaculture Society 42(5): 687-695.
  • Pires, L., Coyle, S., Bright, L. A. and J. Tidwell. 2011. Evaluation of a new high protein distillers grains with soluble for inclusion in diets for channel catfish, Ictalurus punctatus. Posters-at-the-Capitol, p. 67.
  • Pires, L., Coyle, S. D., Bright, L. A. and J. H. Tidwell. 2011. Evaluation of a new high protein distillers grains with solubles for inclusion in diets for channel catfish, Ictalurus punctatus. Association of Research Directors, Inc., 16th Biennial Research Symposium, Atlanta, Georgia, p. 136.
  • Schneider, K. J., Oleyemi, O., Wood, D. R., Coyle, S. D., Gomelsky, B. and J. H. Tidwell. 2011. Initial experiments directed toward genetic improvement of the freshwater prawn Macrobrachium rosenbergii. Aquaculture America, New Orleans, Louisiana, p. 416.
  • Tidwell, J. H., DAbramo, L. R. and S. D. Coyle. 2011. Production of the freshwater prawn in temperate regions: updates and developments. Asian-Pacific Aquaculture & Giant Prawn, Kochin, India, p. 581.
  • Tidwell, J. H., Coyle, S. D. and L. A. Bright. 2011. Production comparison of intraspecific hybrids of the freshwater prawn (Macrobrachium rosenbergii). Association of Research Directors, Inc., 16th Biennial Research Symposium, Atlanta, Georgia, p. 159.
  • Tidwell, J. H., DAbramo, L. R. and S. D. Coyle. 2011. Production of freshwater prawn in temperate regions: Updates and developments. World Aquaculture 2011 Abstracts, Natal, Brazil, p. 1119.
  • Tidwell, J. H. 2011. The evolution of aquaculture academic programs and online course offerings at Kentucky State University. World Aquaculture 2011 Abstracts, Natal, Brazil, p. 1118.
  • Tidwell, J. H. 2011. An overview of freshwater prawn production in the United States. World Aquaculture 2011 Abstracts, Natal, Brazil, p. 1235.
  • Tidwell, J. H. 2011. Marketing of freshwater prawns in the United States. World Aquaculture 2011 Abstracts, Natal, Brazil, p. 1235.
  • VIDEO PUBLICATIONS: Tidwell, J. H., Coyle, S. D. and N. J. Cochran. 2011. Largemouth Bass: Nutrition, Feeds and Feeding. North Central Regional Aquaculture Center (NCRAC). Video Publication 100810. http://www.ncrac.org/nutrition/presentations/tidwell/index.htm
  • Tidwell, J. H., Coyle, S. D. and N. J. Cochran. 2011. Largemouth Bass: Production Technologies for Aquaculture. Southern Regional Aquaculture Center (SRAC). https://srac.tamu.edu/index.cfm/event/CatergoryDetails/whichcategory/ 37/
  • LAY PUBLICATIONS: Tidwell, J. H. and S. D. Coyle. 2011. Post-Harvest Handling of Freshwater Prawns. Southern Regional Aquaculture Center (SRAC) Publication No. 4831.


Progress 01/01/10 to 12/31/10

Outputs
OUTPUTS: Two studies were conducted during the reporting period. The first addressed Objective 4, to evaluate a new high protein form of distiller's grains. Channel catfish remains the nation's largest aquaculture industry but high feed prices have impacted profitability. Ethanol companies have recently developed modified processes to enhance ethanol yield and increase the crude protein content of distiller's grains (DDGS). The Control diet was similar to a commercial diet while experimental diets contained either 20%, 40%, or 40% DDGS with added lysine. After 9 weeks fish fed the 40% DDGS diet without lysine (57.0 g) were significantly smaller than the Control diet (77.7 g). Average harvest weights were significantly higher for fish fed 20% DDGS (86.8 g) than for fish fed either 40% DDGS (57.0 g) or 40% DDGS+Lys (73.7 g). There were no differences in FCR or survivals. These data indicate a 10% growth improvement by addition of 20% DDGS over the Control diet and that the 40% DDGS diet was likely limiting in lysine. The second study addressed Object 6, to compare production characteristics of hybrid crosses of freshwater prawns. Prawn production in the US is based on a small number of founder stock reused for over 20 generations. Two genetic strains of domestic prawns (Texas and Hawaii) and a wild strain (Myanmar) were crossed. Four test crosses were produced and compared with pure Texas strain. After 115 days prawns were harvested from ponds. Survival was significantly higher in the TxH cross than in the TxT, TxM or MxT groups. Specific growth rate (SGR) was significantly higher in the MxT than in the TxM or TxH crosses. In terms of premium sizes, the MxT cross had significantly greater percentage of animals >50 g and > 70 g than the HxT or TxH crosses. Morphotype data indicate that the Myanmar crosses likely had delayed sexual maturation in both sexes. The Myanmar crosses appear to possess positive production traits which might be exploited through further selection. Dissemination activities included presenting these data to several end user groups: Schneider, K., Coyle, S. D., Bright, L. A., Gomelsky, B. and Tidwell, J.H. 2010. Pond grow-out evaluation of intra-specific prawn hybrids. U.S. Freshwater Prawn and Shrimp Growers Association, 10th Annual Meeting, Tunica, Mississippi. December 4. Tidwell, J., Gomelsky, B., D'Abramo, L., Schneider, K., Coyle, S., Wood, D. and Odeyemi, O. 2009. Genetic improvements of prawns: progress report. Ninth Annual Convention of the United States Freshwater Prawn Growers Association. Tunica, MS. December 4. Elliott, L., Coyle, S., Schneider, K. and Tidwell, J. 2009. Comparison of morphological percentages of three different genetic strains of freshwater prawn raised in ponds in Kentucky. Kentucky Minority and Small Farms Conference, Frankfort, KY. Elliott, L., Coyle, S., Schneider, K. and Tidwell, J. 2010. Comparison of morphotype percentages of three different genetic strains of the freshwater prawn raised in ponds in Kentucky. Posters-at-the-Capital, Frankfort, Kentucky, January 28. Tidwell, J. H. 2010. PARTICIPANTS: The Principal Investigator/Project Director for the research is Dr. James Tidwell. He has overall responsibility for the project including design, planning, conduct, analysus, and write up. He also participates in workshop training and interaction with collaborators. Shawn Coyle is the Co-Investigator on the project. He is also actively engaged in design, planning, conduct, analyses and write up. He is particularly involved in the active conduct of the studies. Ms. Leigh Anne Bright is the Research Assistant on the project. While involved in the conduct of the studies her primary responsibilities involve data management to ensure required data are recorded, stored, backed-up, and properly analyzed and reported. TARGET AUDIENCES: Target audiences for this research include existing largemouth bass, channel catfish, freshwater prawn, and tilapia producers and potential producers. Commercial feed manufacturers are also targeted to allow them to significantly reduce their production costs while still producing a high performing feed. Efforts to disseminate science-based knowledge from the project will take two routes. The first is directed at scientists and students. Data are presented at scientific meetings such as Aquaculture America. The other is getting the information to producers in our Extension type format. PROJECT MODIFICATIONS: No major changes have been made.

Impacts
This research is new enough that results have not yet been adopted by end users. Impact on students is also important. Students involved in the research and presentation of results include one undergraduate (Elliot) and three graduate students (Schneider, Odeyemi, and Wood).

Publications

  • Schneider, K. J., Wood, D. R., Odeyemi, O., Coyle, S. D., Bright, L. A. and Tidwell, J. H. 2010. Comparison of three different genetic strains of the freshwater prawn Macrobrachium rosenbergii raised in ponds in Kentucky. Kentucky Aquatic Farming 23:1,9-10.
  • Coyle, S. D., Alston, D. E. and Sampaio, C. M. S. 2010. Nursery system management. Pages 108-126. In Freshwater Prawns: Biology and Farming. Wiley-Blackwell.
  • Tidwell, J. H. 2010. Prawn,Freshwater Shrimp Culture. In Encyclopedia of Animal Science, 2nd edition. Taylor and Francis. Freshwater Prawns: Biology and Farming. Blackwell Science. 544 ppgs.
  • Tidwell, J. H. and DAbramo, L. R. 2010. Growout Systems Culture in Temperate Zones. Pages 180-194. In Freshwater Prawn Culture: The farming of Macrobrachium rosenbergii. 2nd Edition. Wiley-Blackwell.
  • Coyle, S., Bright, L. A. and Tidwell, J. H. 2010. Recent developments in feed training largemouth bass fry. Aquaculture 2010, San Diego, CA, p. 230.
  • Coyle, S., Neal, R. and Tidwell, J. H. 2010. Kentucky experience with marine shrimp bio-flo systems. Aquaculture 2010, San Diego, CA, p. 229.
  • Tidwell, J. H., Coyle, S. D. and Bright, L. A. 2010. Effect of feed pellet characteristics on growth and feed efficiencies of the largemouth bass Micropterus salmoides. Aquaculture 2010, San Diego, CA, p. 1004.


Progress 01/01/09 to 12/31/09

Outputs
OUTPUTS: This project was initiated in October, 2009. Due to the seasonality of the research to be conducted no outputs have yet been generated. PARTICIPANTS: The Principal Investigator/Project Director for the research is Dr. James Tidwell. He has overall responsibility for the project including design, planning, conduct, analysis, and write-up. He also participates in workshop training and interaction with collaborators. Shawn Coyle is the Co-Investigator on the project. He is also actively engaged in design, planning, conduct, analysis and write-up. He is particularly involved in the active conduct of the studies. Ms. Leigh Anne Bright is the Research Assistant on the project. While involved in the conduct of the studies her primary responsibilities involve data management to ensure that required data are recorded, stored, backed-up, and properly analyzed and reported. TARGET AUDIENCES: Target audiences for this research include existing largemouth bass, channel catfish, freshwater prawn, and tilapia producers and potential producers. Commercial feed manufacturers are also targeted to allow them to significantly reduce their production costs while still producing a high performing feed. Efforts to disseminate science-based knowledge from the project will take two routes. The first is directed at scientists and students. Data are presented at scientific meetings such as Aquaculture America. The other is getting the information to producers in our Extension type format. PROJECT MODIFICATIONS: No major changes have been made.

Impacts
This project was initiated in October, 2009. Due to the seasonality of the research to be conducted, no outcomes or impacts have yet been generated.

Publications

  • No publications reported this period