Progress 09/01/09 to 08/31/12
Outputs
OUTPUTS: The mission of the National Animal Health Laboratory Network (NAHLN) is "to provide accessible, timely, accurate, and consistent animal disease laboratory services nationwide and maintain the capacity and capability to respond to a foreign animal disease outbreak and to focus on diseases of livestock, but also including non-livestock species." NAHLN tests are performed according to the NAHLN standard operating procedures and all equipment is carefully monitored and maintained. An extensive Quality System is in place and monitored daily by the Quality Manager, the Director, and staff. Twelve staff are trained for NAHLN testing programs involving 12 diseases and have passed yearly proficiency tests. In the years from September 2009 to August 2012, CSUVDL preformed over 72,000 tests for the NAHLN, including BSE ELISA assays, scrapie IHC tests, CWD ELISA assays, CWD IHC tests, Avian Influenza qPCR, Newcastle disease virus qPCR, classical swine fever qPCR, vesicular stomatitis complement fixation assays, piroplasmosis ELISA assays, foot and mouth disease qPCR, rinderpest qPCR, and H1N1 qPCR The CSUVDL is also a Laboratory Response Network approved laboratory through the CDC. We are approved to preform testing for Coxiella burnetti, Brucella spp., Yersinia pestis, Bacillus anthracis, and Francisella tularensis. For the period September 2009 until August 2012, we preformed over 600 tests, including tests for Coxiella, Yersinia pestis, Francisella tularensis, Bacillus anthracis and Brucella sp. The CSUVDL has an advanced Information Technology (IT) system using the STARLIMS system and HL7 messaging . Six staff and the Director take and passed the NAHLN IT security clearance test annually, most recently in January 2013. One faculty member has traveled nationally and internationally and has provided laboratory-based training or has assisted them in developing capabilities for trans boundary animal disease detection and laboratory diagnostics. She has also given presentations on laboratory networking using the NAHLN system as an example. The Director is co-chair of the AAVLD/USAHA NAHLN Special Committee. This committee has monthly calls and has composed and sent multiple requests/resolutions to USDA . In August 2010, CSUVDL hosted an inter-laboratory tabletop exercise in FMD that included Wyoming, Nebraska, Montana, and USDA officials from the Western Region. In August of 2012, CSUVDL diagnosed and responded to an outbreak of Anthrax in Colorado and subsequently participated in a CDC laboratory exercise of an Anthrax outbreak. CSU VDL has entered information in the NAHLN portal and used the FAZD laboratory capacity estimator. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
The Colorado State University Veterinary Diagnostic Laboratories (CSUVDL) are able to meet the mission of NAHLN by virtue of our quality system, faculty, and staff trained for detecting foreign animal and emerging diseases, advanced information Technology system including capabilities for NAHLN messaging, proven NAHLN testing capacity including equipment and BSL2 and BSL3 laboratory space, inter-laboratory support, and outreach activities. Training for responding to foreign animal and emerging diseases is part of the Quality System. Twelve staff are trained for NAHLN testing programs involving 12 diseases. CSUVDL can transmit HL7 messages for classical swine fever. Staff also use the USDA system for data input for other NAHLN diseases. Laboratory equipment, including high-throughput equipment is in place and maintained. NAHLN testing is done in a 2,000 square foot BSL3 space that has been inspected by the CDC and USDA for Select Agent approval. CSU VDL participated in the NAHLN negative cohort study for Foot and Mouth disease. One Faculty member has traveled nationally and internationally and had provided laboratory-based training or has assisted in developing capabilities for trans boundary animal disease detection and laboratory diagnostics. In 2010, she coordinated and developed a two-week training course for the Iraqi National Animal Health Laboratory System in Iraq, and a one-week training course for the National Veterinary Laboratory System in the Lao People's Democratic Republic. She gave lectures on avian influenza in Indonesia and China. The Director is the co chair of the AAVLD/USAHA NAHLN Special Committee. Drs. Barb Powers and Kristy Pabilonia routinely contribute to outreach and service activities throughout the state, working with the Colorado Department of Agriculture, Colorado Department of Public Health and Environment, Colorado Division of Wildlife, Colorado Veterinary Medical Association, Colorado Cattlemen's Association, and Colorado Livestock Association. Presentations or meetings with these groups occur on a regular basis and information about the NAHLN and its activities is presented and distributed. Dr. Powers serves on the Government Coordinating Council for Food and Agriculture. Dr. Pabilonia teaches veterinary students about emerging and foreign animal diseases and discusses the NALHN in her lectures. She is an instructor for the Colorado Department of Agriculture Foreign Animal Disease training course. Dr. Powers gave a lecture on Biosafety in Veterinary Diagnostic Laboratories to the American Biological Safety Association Annual Meeting. The laboratory diagnosed and responded to an outbreak of Anthrax in Colorado, the first in 30 years in Colorado, using CDC protocols.
Publications
- No publications reported this period
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Progress 09/01/10 to 08/31/11
Outputs
OUTPUTS: The mission of the National Animal Health Laboratory Network (NAHLN)is "to provide accessible, timely, accurate and consistent animal disease laboratory services nationwide and maintain the capacity and capability to respond to a foreign animal disease outbreak and to focus on diseases of livestock, but also including non-livestock species." NAHLN tests are performed according to the NAHLN standard operating procedures and all equipment is carefully monitored and maintained. An extensive Quality System is in place and monitored daily by the Quality Manager, the Director, and staff. Twelve staff are trained for NAHLN testing programs involving 12 diseases and have passed yearly proficiency tests. In the year from April 1, 2010 until March 31, 2011, CSUVDL performed 6289 BSE ELISA assays, 2574 scrapie IHC tests, 7139 CWD ELISA assays, 3733 CWD IHC tests, 1188 Avian Influenza qPCR, 4 Newcastle disease virus qPCR, 601 classical swine fever qPCR, 0 vesicular stomatitis complement fixation assays, 1231 piroplasmosis ELISA assays, 550 foot and mouth disease qPCR, 230 rinderpest qPCR, and 7 H1N1 qPCR. The CSUVDL is also a Laboratory Response Network approved laboratory through CDC. We are approved to perform testing for Coxiella burnetii, Brucella spp., Yersinia pestis, Bacillus anthracis and Francisella tularensis. For the period April 1, 2010 until March 30, 2011, we performed 89 Coxiella, 30 Yersinia pestis, 26 Francisella tularensis and 6 Brucella tests. The CSUVDL has an advanced Information Technology (IT) system that is undergoing an extensive upgrade using the STARLIMS system and HL7 messaging is operational. Six staff and the Director take and passed the NAHLN IT security clearance test annually, most recently in January 2011. One faculty member has traveled nationally and internationally and has provided laboratory-based training or has assisted them in developing capabilities for transboundary animal disease detection and laboratory diagnostics. She has also given presentations on laboratory networking using the NAHLN system as an example. The Director serves on the AAVLD/USAHA NAHLN Special Committee and is the co-chair with USAHA co-chair Dr. Harry Snelson. This committee has monthly calls and has composed and sent multiple requests/resolutions to USDA. In August 2010, CSUVDL hosted an inter-laboratory tabletop exercise in FMD that included Wyoming, Nebraska, Montana and USDA officials from the Western Region. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
The Colorado State University Veterinary Diagnostic Laboratories (CSUVDL) are able to meet the mission of NAHLN by virtue of our quality system, faculty and staff trained for detecting foreign animal and emerging diseases, advanced Information Technology system including capabilities for NAHLN messaging, proven NAHLN testing capacity including equipment and BSL2 and BSL3 laboratory space, inter-laboratory support, and outreach activities. Training for responding to foreign animal and emerging diseases is part of the Quality System. Twelve staff are trained for NAHLN testing programs involving 12 diseases. CSUVDL can transmit HL7 messages for classical swine fever. Staff also use the USDA system for data input for other NAHLN diseases. Laboratory equipment, including high-throughput equipment is in place and maintained. NAHLN testing is done in a 2000 sq ft BSL3 space that has been inspected by the CDC and USDA for Select Agent approval. One Faculty member has traveled nationally and internationally and has provided laboratory-based training or has assisted in developing capabilities for transboundary animal disease detection and laboratory diagnostics. In 2010, she coordinated and developed a two-week training course for the Iraqi National Animal Health Laboratory System in Iraq, and a one-week training course for the National Veterinary Laboratory System in the Lao People's Democratic Republic. She gave lectures on avian influenza in Indonesia and China. The Director serves on the AAVLD/USAHA NAHLN Special Committee and is the co-chair for USAHA. Drs. Barb Powers and Kristy Pabilonia routinely contribute to outreach and service activities throughout the state, working with the Colorado Department of Agriculture, Colorado Department of Public Health and Environment, Colorado Division of Wildlife, Colorado Veterinary Medical Association, Colorado Cattlemen's Association and Colorado Livestock Association. Presentations or meetings with these groups occur on a regular basis and information about the NAHLN and its activities is presented and distributed. Dr. Powers serves on the Government Coordinating Council for Food and Agriculture. Dr. Pabilonia teaches veterinary students about emerging and foreign animal diseases and discusses the NAHLH in her lectures. She gave lectures on influenza in Denver, at the Colorado Veterinary Medical Association Annual Meeting and to poultry and 4-H organizations. She was an instructor for the Colorado Department of Agriculture Foreign Animal Disease training course. Dr. Powers gave a lecture on Biosafety in Veterinary Diagnostic Laboratories to the American Biological Safety Association Annual Meeting.
Publications
- No publications reported this period
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Progress 09/01/09 to 08/31/10
Outputs
OUTPUTS: CSUVDL has standard operating procedures in place and technicians trained for BSE, Scrapie, CWD, Avian Influenza, Newcastle Disease virus, Classical Swine Fever, Foot and mouth disease, Pseudorabies, Swine Influenza, Piroplasmosis and vesicular stomatitis testing. In year July 1, 2009 until May 30, 2010 CSUVDL performed 12,973 BSE tests, 10,998 Scrapie tests, 10,870 CWD tests, 1,660 Avian Influenza tests, 3 Newcastle disease virus tests, 465 classical swine fever tests, 5,447 vesicular stomatitis tests, and 146 Piroplasmosis tests. Moved into a newly constructed 2000 sqft BSL3 facility plus a 1000 sqft TSE testing area. USDA and CDC inspection of the new facility has been completed. PARTICIPANTS: Laboratory Director, IT personnel, QA management team, faculty, and laboratory technicians TARGET AUDIENCES: Veterinarians, Produce groups, and AAVLD members PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
Staff performing NAHLN testing has been trained by either USDA staff or by Ms. Christina Weller who has completed the "train the trainer" program. Ms. Weller has trained technicians within CSUVDL and from other states. All staff performing NAHLN testing have taken and passed yearly proficiency tests administered by the national veterinary services laboratories. Most pathologists staffed in the CSUVDL have taken the USDA Foreign Animal Disease Pathologist course and are prepared to detect foreign animal disease in animals submitted for necropsy. K. Pabilonia and Colleen Duncan are trained USDA Foreign Animal Disease Diagnosticians. The Case Coordinator, Dr. John Maulsby, is a former state veterinarian and also has experience in recognizing foreign animal diseases, including international field experience. All faculty regularly attend national conferences and monitor informational sources and are, therefore, ready and able to recognize emerging diseases. Faculty and staff regularly communicate with each other regarding disease conditions observed and Dr. Maulsby coordinates multi-section cases and is, therefore, in a position to detect unusual disease trends. Mr.Jay Kammerzell has assisted other laboratories to develop NAHLN messaging. Dr. Kristy Pabilonia has traveled nationallly and internationally and trained other laboratories or assisted them in developing abilities for transboundary animal disease detection and laboratory diagnostics and has given persentations on laboratory networking using the NAHLN system as as example. Dr. Barb Powers serves on the AAVLD/USAHA NAHLN Special committeee and is the co-chair with USAHA co-chair Dr. Richard Breitmeyer. This committee has monthly calls and has composed and sent multiple requests/resolutions to USDA. Dr. Powers also serves on the Government Coordinating Council, representing AAVLD and the NAHLN. This group has monthly conference calls and meets face-to-face quarterly. Dr.s Barb Powers and Kristy Pabilonia routinely contribute to outreach and service activities through out the state, working with the Colorado Department of Agriculture, Colorado Department of Public Health and Environment, Colorado Division of Wildlife, Colorado Veterinary Medical Association, Colorado Cattlemen's Association and Colorado Livestock Association. Presentatons or meetings with these groups occur and information about the NAHLN and its activities is presented and distributed. Both Dr.s Powers and Pabilonia also give national presntations concerning NAHLN activities.
Publications
- No publications reported this period
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