Progress 09/01/09 to 08/31/12
Outputs
OUTPUTS: During this reporting period Wyoming State Veterinary Laboratory (WSVL) personnel have been proficiency tested and approved to conduct testing for the following programmatic diseases: four for avian and swine influenza, four for classical swine fever, four for chronic wasting disease and scrapie, three for epizootic Newcastle disease, two for foot and mouth disease, and two for vesicular stomatitis virus. To date, additional testing activities have been 750 samples from primarily domestic avian species for avian influenza, mainly in conjunction with the State Veterinarian, Dr. Jim Logan, and Wyoming Livestock Board. During this period the WSVL received provisional full accreditation from the American Association of Veterinary Laboratory Diagnosticians Accreditation Committee, ensuring that quality assurance and quality control procedures acceptable to the NAHLN are in place at this laboratory. Results of a second site visit in June 2012 are still pending. As stated in previous reports, the WSVL has over 2000 square feet of newly constructed biosafety level-3 laboratory space. During the current reporting period, the WSVL has established operational protocols for operation of the new space, have equipped these spaces, and a CDC audit was conducted in August 2012; results of the audit are currently pending. Finally during this reporting period LIMsPro, the WSVL data management system is capable of NAHLN messaging. This work has been disseminated at state meetings of the general public, livestock and wildlife officials, and practicing veterinarians as well as the laboratory's quarterly newsletter and other lay publications. PARTICIPANTS: WSVL personnel involved in NAHLN testing and associated activities are: Myrna Miller, DVM, PhD, veterinary virologist; Will Laegreid, DVM, PhD, Laboratory Director; Todd Cornish, DVM, PhD, DACVP, veterinary pathologist; Donald L. Montgomery, DVM, PhD, DACVP, veterinary pathologist; Jeffery Adamovicz, PhD, immunologist and BSL-3 Scientific Director; Kenneth Mills, PhD, veterinary bacteriologist; Mark Davidson, information management specialist and quality assurance coordinator; and the following WSVL technical staff involved in NAHLN testing: Jacque Cavender, Marce Vasquez, Breanna Bonner, Rebecca Wills, Rebecca Ashley, Mercedes Thelen, and Joan Edwards. Partner organizations include the Wyoming Livestock Board and State Veterinarian Jim Logan, DVM; the Wyoming Department of Public Health and veterinarian Karl Musgrave, DVM, PhD; and Wyoming Game and Fish Department, Wildlife Disease Laboratory. TARGET AUDIENCES: Target audiences include USDA APHIS including national NAHLN program professionals and staff, regional and national veterinary diagnostic laboratories, domestic animal and wildlife managers, practicing veterinarians, and the general public. PROJECT MODIFICATIONS: During this reporting period, Will Laegreid became the new WSVL Director, replacing D.L. Montgomery who returned to work as a WSVL pathologist, and the employment of Jeffery Adamowicz as immunologist and, importantly, scientific director of the BSL-3 facility. There have been no other major programmatic changes.
Impacts
Activities and outcomes reported above are illustrative of WSVL's commitment to NAHLN testing for the surveillance of high impact animal pathogens in the United States. Resources provided by the NAHLN, partial salary support for one virology technician and annual maintenance and calibration for smart cyclers used in NAHLN testing, are invaluable in helping the WSVL maintain this capability. Pending approval of new biosafety level-3 facilities by the CDC, additional capacity will be added for conduct of testing for high impact animal pathogens in the region and Rocky Mountain west.
Publications
- No publications reported this period
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Progress 09/01/10 to 08/31/11
Outputs
OUTPUTS: During this reporting period, NAHLN funds were used to provide partial salary support for one virolgy technician and for calibration and annual maintenance of two Cepheid smart cyclers used in NAHLN testing. Also during this period, four technicians were proficiency tested for avian influenza, swine influenza including pandemic H1N1, and exotic Newcastle disease. Four technicians were tested and approved to perform foot and mouth disease and classical swine fever testing. For SIV, 3 samples were tested, and AIV tests were performed on 220 samples. New construction and renovation was completed in the last year that provides approximately 3,257 square feet of biosafety level-3 laboratory and support spaces. Additionally, the Wyoming State Veterinary Laboratory (WSVL)adopted and implemented a new LIMS that is scheduled to be capable of NAHLN messaging by December 31, 2011. This work has been disseminated at state meetings of the general public, livestock and wildlife officials, and practicing veterinarians as well as WSVL's quarterly newsletter and other lay publications. PARTICIPANTS: Donald L. Montgomery DVM, PhD, DACVP, Department Head and Director; Ken Mills PhD, bacteriologist, Myrna Miller DVM, PhD, virologist; Jackie Cavender, Marce Vasquez, Rebecca Ashley, Mercedes Thelen, laboratory technicians. TARGET AUDIENCES: National Animal Health Laboratory Network (NAHLN) national program staff; Wyoming Livestock Board; Wyoming Department of Health; Wyoming Department of Agriculture; Wyoming Game and Fish Department. PROJECT MODIFICATIONS: Construction of expanded biosafety level-3 laboratories as well as general support spaces and enhanced electronic facility security have been completed. Standard Operating Procedures (SOP) have been written for the new biosafety level-3 laboratories, and the new laboratories are being equipped. The new facilities and SOPs will be evaluated and inspected by CDC for modifications and certification during the upcoming year.
Impacts
The work conducted during this reporting period will reinforce the Wyoming State Veterinary Laboratory's commitment to support national efforts in surveillance for diseases of national and international importance, not only for those with the potential to impact domestic animal and wildlife health but public health as well. Construction of biosafety level-3 laboratory space and ability to attract and hire professionals dedicated to diagnostic testing should help insure the WSVL's value in NAHLN testing.
Publications
- No publications reported this period
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Progress 09/01/09 to 08/31/10
Outputs
OUTPUTS: During this reporting period, NAHLN funds were used to provide partial salary support for one virolgy technician and for calibration and annual maintenance of two Cepheid smart cyclers used in NAHLN testing. Also during this period, three technicians were proficiency tested for avian influenza, swine influenza including pandemic H1N1, and exotic Newcastle disease. Four technicians were tested and approved to perform foot and mouth disease and classical swine fever testing. For SIV, 6 samples were tested and 186 AIV tests were performed. Other accomplishments related to or in support of the NAHLN include the hiring of a veterinary virologist who will assume responsibilities for testing of NAHLN samples of viral origin. Additionally, new construction and renovation were begun to increase laboratories and to provide approximately 3,257 square feet of biosafety level-3 laboratory and support spaces; completion is December 2010. Additionally, the Wyoming State Veterinary Laboratory has adopted a new LIMS capable of NAHLN messaging that should go live in October 2010. This work has been disseminated at state meetings of the general public, livestock and wildlife officials, and practicing veterinarians as well as the laboratory's quarterly newsletter and other lay publications. PARTICIPANTS: Donald L. Montgomery DVM, PhD, DACVP, Department Head and Director; Ken Mills PhD, bacteriologist, Myrna Miller DVM, PhD, virologist; Jackie Cavender, Marce Vasquez, Rebecca Ashley, Mercedes Thelen, laboratory technicians. TARGET AUDIENCES: NAHLN national program staff; Wyoming Livestock Board; Wyoming Department of Health; Wyoming Department of Agriculture; Wyoming Game and Fish Department. PROJECT MODIFICATIONS: Construction of expanded biosafety level-3 laboratories and dedicated as well as general support spaces and enhanced electronic facility security will require efforts at CDC facility certification and modifications to existing laboratory and NAHLN SOPs during the upcoming year.
Impacts
The work conducted during this reporting period will reinforce the Wyoming State Veterinary Laboratory's commitment to support national efforts in surveillance for diseases of national and international importance, not only for those with the potential to impact domestic animal and wildlife health but public health as well. Construction of biosafety level-3 laboratory space and ability to attract and hire professionals dedicated to diagnostic testing should help insure the laboratory's value in NAHLN testing.
Publications
- No publications reported this period
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