Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: We produced transgenic poplar plants with altered hormone contents. Characterization of transgenic poplar plants suggests that growth and developmental patterns and chemical compositions of wood were also altered. We also worked on refinement of the gene deletor technology. Further evaluation experiments of the gene deletor technology demonstrate that the technology works well under field conditions. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Our results suggest that manipulation of plant hormone contents in planta could lead to alterations in growth and developmental characters and chemical compositions of woody plants. Evaluation of the gene deletor technology provides a basis for field application of the technology. Our work also suggests that the hormonal genes we used and the gene deletor system we developed may be of some significance to bioenergy applications.
Publications
- Li, Y and Duan, H (2011): Molecular approaches for transgene containment and their potential applications in horticultural crops. In: Transgenic horticultural crops: challenges and opportunities. Mou. B. and Scorza R. (ed.) CRC Press. 289-299.
- Li, S.Y. R. Srivastava, S. L. Suib, Y. Li, R. S. Parnas (2011): Performance of batch, fed-batch, and continuous A-B-E fermentation with pH-control. Bioresource Technology 102: 4241-4250
- Si-Yu Li, James D. Stuart, Yi Li and Richard S. Parnas (2010): The feasibility of converting Cannabis sativa L. oil into biodiesel. Bioresource Tech. 101: 8457-8460.
- Ye, X. V. Busov, N. Zhao, R. Meilan, L. M. McDonnell, H. D. Coleman, S. D. Mansfield, F. Chen, Y. Li, and Z-M Cheng (2011). Transgenic Populus trees for forest products, bioenergy, and functional genomics. Critical Reviews in Plant Sciences. 30: 415-434.
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Progress 08/01/09 to 09/30/11
Outputs
OUTPUTS: Transgenic poplar plants with altered hormone contents have been produced. Characterization of these transgenic poplar plants revealed that shoot and root growth and developmental patterns and chemical compositions of wood were drastically altered. We also worked on refinement of the gene deletor technology. We have showed that modification of the previously published gene deletor system can further improve its efficiency. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Our results suggest that manipulation of plant hormone contents in planta might be useful to improve bioenergy crop plants. Improvement of gene deletor technology may facilitate applications of the technology in crop plants. Our work also suggests that the hormonal genes we used and the gene deletor system we developed may be of some significance to bioenergy applications.
Publications
- No publications reported this period
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: We have produced transgenic poplar plants with altered hormone contents. We have also been working on refinement of the gene deletor technology. Characterization of transgenic poplar plants suggests that growth and developmental patterns and chemical compositions of wood have also been altered. Further evaluation experiments of the gene deletor technology demonstrate that the technology works well under field conditions. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Our results suggest that manipulation of plant hormone contents in plants could lead to alterations in growth and developmental characters and chemical compositions of woody plants. Evaluation of the gene deletor technology provides a basis for field application of the technology. Our work suggest that the hormonal genes we used and the gene deletor system we developed may be of some significance to bioenergy applications.
Publications
- Kausch A, Hague J, Oliver M, Li Y, Daniell H, Mascia P, Watrud L, and C. Neal Stewart, Jr. 2010. Transgenic biofuel feedstocks and strategies for biocontainment. Biofuels. In Press. In the inaugural issue.
- Osburn L., Yang X., Li Y., and ZM Cheng. 2009. Micropropagation of Japanese Honeysuckle, Lonicera japonica, and Amur Honeysuckle L. maackii by Shoot Tip Culture. J. Environ. Hort. 27, 195-199
- Hong S. Moon, H. S., Li, Y., and C. Neal Stewart C. N. 2009. Keeping the genie in the bottle: transgene biocontainment by excision in pollen. Trends in Biotechnology. 28, 3-8.
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Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: We have produced transgenic poplar plants with altered hormone contents. Characterization of some of these transgenic plants at the molecular, physiological and morphological levels suggests that growth and developmental characters and chemical compositions of wood have also been altered. PARTICIPANTS: Yi Li (UConn, PI): Supervise the project and perform the proposed experiemnts. No salary funds are provided to this project and therefore no other people working on this project. TARGET AUDIENCES: Scientists and students at university/college and research institutions working in the similar research areas; Bioenergy industry. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Our results suggest that manipulation of plant hormone contents in planta could lead to alterations in growth and developmental characters and chemical compositions of woody plants and that may be for some significance to bioenergy applications.
Publications
- Ye, X., Kang, B., Osburn, L., Li, Y., Cheng, Z. 2009. Identification of the flavin-dependent monooxygenase-encoding YUCCA gene family in Populustrichocarpa and their expression in vegetative tissues and in response to hormone and environmental stresses. Plant Cell, Tissue and Organ Culture (PCTOC: J. of Plant Biotechnology) 97:271-283
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