Progress 06/01/09 to 05/31/14
Outputs
Target Audience:The Scientific Community in Food Science and Nutrition were reached with peer reviewed research publications, invited oral presentations, press releases and scientific discussions Undergraduate students in Food Science and Nutrition were educated in this research area in practical laboratory applications and therory Graduate Students in Food Science and Nutrition were educated in this research area in practical laboratory applications and therory The Public was reached with scientific press releases Food and Dietary Supplement Industry was reached with scientific reports and press releases and scientific information International visiting scholars: were educated in this research area in practical laboratory applications and therory Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The above listed research provided training opportunities for: - six undergraduate students in nutrition and food science in practical laboratory training and critical thinking in research as well as life-long learning approaches - four Ph.D. students in Nutrition and Food Science have been training in the above listed research as well as critical thinking in research as well as life-long learning approaches - three postdoctoral researchers have been trained in the above listed research as well as experienced critical thinking in research as well as life-long learning approaches - Two international scholars have been trained in practical laboratory training and critical thinking in research as well as lifelong learning approaches How have the results been disseminated to communities of interest?1. Scientific Peer Reviewed Publications 2. Press Releases 3. Abstracts for Posters and Oral Presesntations at International Conferences 4. as Knowledge dissesminated in the classroom for undergraduate and graduate students What do you plan to do during the next reporting period to accomplish the goals?It is planned to continue to complete the above listed three objectives with a major focus on microbial metabolism of bioactive plant compounds and inter-individual differences
Impacts
What was accomplished under these goals?
Objectives: 1. To determine the antioxidant properties of polyphenolic fruit extracts and contained compounds. 2. To investigate the basic anti-cancer, anti-inflammatory, anti-atherosclerotic, and antioxidant properties of contained compounds in cell culture and to assess underlying molecular mechanisms 3. To translate in vitro research into clinical studies in animals and human subjects. Antioxidant and anti-inflammatory activities have been investigated in vitro and in vivo where underlying mechanisms have been investigated Findings have been translated into human clinical pilot trials. This project has been completed as planned..
Publications
|
Progress 01/01/13 to 09/30/13
Outputs
Target Audience: Scientific Community, Nutrition and Food Science Community, State of Texas Consumers Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest? Agrilife press report on mangos. What do you plan to do during the next reporting period to accomplish the goals? Human clinical trial on absorption of mango , data analysis of inflammation markers in subjects with metabolic syndrom that consumed acai juice, animal study with mango polyphenolics, complete IBD study with mango, cell culture with cancer and non-cancer cells to determine the underlying molecular mechanisms.
Impacts
What was accomplished under these goals?
Goals #1, 2 and 3 have been partially accomplished and will be continued in 2014. A new plan will be written in 2015.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Garc�a-P�rez E*, Noratto GD*, Garc�a-Lara S, Guti�rrez-Uribe JA, Mertens-Talcott SU. Micropropagation Effect on the Anti-carcinogenic Activitiy of Polyphenolics from Mexican Oregano (Poliomintha glabrescens Gray) in Human Colon Cancer Cells HT-29. Plant Foods Hum Nutr. 2013 Feb 23. Plant Foods Hum Nutr. 2013 Jun;68(2):155-62.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Del Follo-Martinez A*, Li X, Banerjee N*, Safe S, Mertens-Talcott SU. Resveratrol and Quercetin in Combination Have Anti-Cancer Activity in Colon Cancer Cells and Repress Oncogenic microRNA-27a. Nutr Cancer. 2013;65(3):494-504
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Banerjee N*, Kim H*, Talcott ST, Pfent C.M, Mertens-Talcott SU. Pomegranate Polyphenolics Suppressed Azoxymethane-Induced Colorectal Aberrant Crypt Foci (ACF) and Inflammation: Possible role of miR-126/VCAM-1 and miR-126/PI3K/AKT/mTOR.
Carcinogenesis. 2013 Aug 29. [Epub ahead of print]
|
Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: Peer-reviewed manuscripts were generated in the areas of molecular biology, nutrition and food chemistry in reputable journals. For industry-sponsored projects, technical reports were generated for industry. Additionally, the gained knowledge was shared with researchers and consumers at numerous conferences and research conventions within the US and additionally generated an internation collaboration with the Universidad Federale Vicosa, Brazil. PARTICIPANTS: Graduate students: Nivedita Banerjee, Hyemee Kim, Enrique Garcia, Postdoctoral researcher: Giuliana Noratto, Hercia Stampini Undergraduate students: Andrea Roque, Lilly McAllister. All students contributed to the performance of the research projects. The Institute for Obesity provided a research platform. Funding was received from the NIH, USDA, and the National Mango board which funded the research performed on mangos. TARGET AUDIENCES: Students, communication with colleagues, industry partners, funding agencies (USDA, NIH, AHA and others). PROJECT MODIFICATIONS: Nothing significant to report during this reporting period. PARTICIPANTS: PARTICIPANTS: Graduate students, Postdoctoral researcher, Undergraduate students. All students contributed to the performance of the research projects. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
Results from research studies were communicated at different levels. Mango, fruit were evaluated in their anti-inflammatory activity in differnt cell types and in animals, and a pilot absorption study has been performed in healthy human subjects. Moreover one colon cancer model with mango and pomegranate was tested in rats. These findings were communicated to the mango industry. Several in vitro studies were performed investigating the anti-inflammatory mechanisms of other polyphenolic-rich fruits. Findings from these studies were communicated to interested industry as well as research-colleagues and students and also are expected to result in peer-reviewed publications. A human clinical trial with acai in obese subjects has started and is in progress This study performed in the anti-cancer effects of botanicals should generate a follow-up study with clinical relevance.
Publications
- Del Follo-Martinez A, Li X, Banerjee N, Safe S, Mertens-Talcott SU. Resveratrol and Quercetin in Combination Have Anti-Cancer Activity in Colon Cancer Cells and Repress Oncogenic microRNA-27a. Nutrition and Cancer 2012, in press. manuscript ID N-C-01-11-0971
- Angel-Morales G-, Noratto GD-, and Mertens-Talcott SU. Standardized curcuminoid extract (Curcuma longa l.) Decreases gene expression related to inflammation and interacts with associated microRNAs in human umbilical vein endothelial cells (HUVEC). Food Funct. 2012 Sep 12. PMID-22972459
- Angel-Morales G, Noratto G, Mertens-Talcott SU. Red wine polyphenolics reduce the expression of inflammation markers in human colon-derived CCD-18Co myofibroblast cells- Potential role of microRNA-126. Food Funct. 2012 Jul 27-3(7)-745-52. Epub 2012 Jun 27.
- Mertens-Talcott SU, Noratto GD, Li X, Angel-Morales G, Bertoldi MC, Safe S. Betulinic acid decreases ER-negative breast cancer cell growth in vitro and in vivo: Role of Sp transcription factors and microRNA-27a-ZBTB10. Mol Carcinog. 2012 Mar 7. doi- 10.1002/mc.21893.
|
Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: OUTPUTS: Outputs for these projects were generated in several ways. Peer-reviewed manuscripts were generated in the areas of molecular biology, nutrition and food chemistry in reputable journals. For industry-sponsored projects, technical reports were generated for industry. Additionally, the gained knowledge was shared with researchers and consumers at numerous conferences and research conventions within the US and additionally generated an internation collaboration with the Universidad Federale Vicosa, Brazil. PARTICIPANTS: Graduate students: Armando Del Follo, Gaby Angel, Emily Townsley; Nivedita Banerjee, Postdoctoral researcher: Giuliana Noratto, Undergraduate students: Andrea Roque and undergraduate research fellows: Julia Nelson, Benjamin Walker, Matthew Feltman. All students contributed to the performance of the research projects. The Institute for Obesity provided a research platform. Funding was received from the NIH, USDA, and the National Mango board which funded the research performed on mangos. TARGET AUDIENCES: Students, communication with colleagues, industry partners, funding agencies (USDA, NIH, AHA and others). PROJECT MODIFICATIONS: Nothing significant to report during this reporting period. PARTICIPANTS: PARTICIPANTS: Graduate students: Armando Del Follo, Gaby Angel, Emily Townsley; Nivedita Banerjee, Postdoctoral researcher: Giuliana Noratto, Undergraduate students: Andrea Roque and undergraduate research fellows: Julia Nelson, Benjamin Walker, Matthew Feltman. All students contributed to the performance of the research projects. The Institute for Obesity provided a research platform. Funding was received from the NIH, USDA, and the National Mango board which funded the research performed on mangos. TARGET AUDIENCES: Students, communication with colleagues, industry partners, funding agencies (USDA, NIH, AHA and others). PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Results from research studies were communicated at differnt levels. Mango, fruit were evaluated in their anti-inflammatory activity in differnt cell types and in animals. Moreover one cancer model with mango and pomegranate was tested in mice. These findings were communicated to the mango industry. Several in vitro studies were performed investigating the anti-inflammatory mechanisms of other polyphenolic-rich fruits. Findings from these studies were communicated to interested industry as well as research-colleagues and students and also are expected to result in peer-reviewed publications. Moreover, two human clinical trials in the areas of absorption of polyphenolics, inflammation and cancer have started within fruitful interdisciplinary collaborations. Studies performed in the area of inflammation and whole grain will have a significant influence on the large number of obese individuals in the US, and also are expected to influence product development and research in this area. The study performed in the anti-cancer effects of botanicals should generate a follow-up study with clinical relevance.
Publications
- Peer Reviewed Publications 2011:Del Follo-Martinez A, Li X, Banerjee N, Safe S, Mertens-Talcott SU, 2011. Resveratrol and Quercetin in Combination Have Anti-Cancer Activity in Colon Cancer Cells and Repress Oncogenic microRNA-27a. Accepted by Nutrition and Cancer Journal pending minor manuscript revisions, 2011. manuscript ID NC-01-11-0971
- Abstracts for oral and poster presentations - 2011: Noratto G., Mertens-Talcott SU. 2011 , Curcuminoids decreases drug resistance in SW-480 colon cancer cells by targeting the miR-27a-ZBTB10-Sp axis. Annual CPRIT conference Austin TX, November 2011.
- Noratto G, Li X, Angel-Morales G, Safe S, Mertens-Talcott, SU. Reduced expression of pro-invasive genes in xenographs of MDA-MB-231 breast cancer cells by Betulinic Acid is mediated by down-regulation of microRNA-27a. Federation of American Societies for Experimental Biology (FASEB), Experimental Biology Annual Meeting, Washington D.C., April 2011.
- Banerjee N, Noratto G, Mertens-Talcott SU Pomegranate Extract has Chemopreventive Effects in Colon Cancer Involving MicroRNA 21 and MicroRNA 126a. Society of Toxicology (SOT) Annual Conference, Washington, DC, March 2011
- Noratto G, Mertens-Talcott SU. Curcumin decreases inflammation in normal cells and exert chemopreventive effect on colon cancer cells through regulation of key microRNAs. Society of Toxicology Annual Meeting Washington D.C. March, 2011
- Invited Oral Presentations 2011 Mertens-Talcott SU, Anti-inflammatory Effects of Polyphenolics in Cowpeas. Future Implications for Increased Utilization. Increased Utilization of Cowpea to Promote Health and Food Security in Kenya Workshop, Nairobi, Kenya, May 2011
- Giuliana Noratto, Gabriela Angel-Morales, and Mertens-Talcott SU 2011. 2011.Polyphenolics from acai (Euterpe Oleracea M.) and red muscadine grape (Vitis Rotundifolia) protect human umbilical vascular endothelial cells (HUVEC) from glucose- and LPS-induced Inflammation and target microRNA-126. J Agric Food Chem. 2011 Jul 27;59(14):7999-8012.
- Noratto G, Kim YM, Talcott ST, Mertens-Talcott SU. 2011.Flavonol-rich fractions of yaupon holly leaves (Ilex vomitoria Aquifoliaceae) induce microRNA-146a and have anti-inflammatory and chemopreventive effects in intestinal myofribrobrast CCD-18Co cells. Fitoterapia. 2011 Jun; 82(4):557-69
- Manthey JA, Cesar TB, Jackson E, Mertens-Talcott SU. 2011. Pharmacokinetic Study of Nobiletin and Tangeretin in Rat Serum by High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry. J Agric Food Chem., 2011 Jan; 59(1):145-51
|
Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: Objective 1: Different varieties of fruits either natively grown in the US or imported tropicals were used for the preparation of extracts with a focus of varieties relevant to the State of Texas. Polyphenolic extracts and fractions of these were prepared as established in the principal investigator's laboratory. Methodology: Detailed chemical analysis was performed with HPLC-PDA and HPLC-MSn. Objective 2: Cell culture models were used in order to investigate the basic anti-cancer, anti-inflammatory, anti-atherosclerotic, and antioxidant properties of polyphenolic compounds and to assess underlying molecular mechanisms, where many extracts demonstrated anti-inflammatory and anti-cancer properties in physiological concentrations. Objective #3: Animal models (a) Male athymic nude mice and b) Zucker rats have been utilized to determine the anti-inflammatory and anti-cancer effects of botanical extracts. Several of the extracts have anti-inflammatory and anti-cancer properties via the proposed mechanisms. PARTICIPANTS: PARTICIPANTS: Graduate students: Armando Del Follo, Gaby Angel, Emily Townsley; Sal Bertucci, Clint Daily. Postdoctoral researcher: Giuliana Noratto. Undergraduate students: Alex Bass, all contributed to the performance of the research projects. The Institute for Obesity provided a research platform in supported of the research. Funding was received from the NIH, TDA, private sponsors and the National Mango board which funded the research performed on mangos. TARGET AUDIENCES: TARGET AUDIENCES: Students, communication with colleagues, industry partners, funding agencies (USDA, NIH, AHA and others). PROJECT MODIFICATIONS: PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
OUTPUTS: Outputs for these projects were generated in several ways. Peer-reviewed manuscripts were generated in the areas of molecular biology, nutrition and food chemistry in reputable journals.For industry-sponsored projects, technical reports were generated for industry. These outcomes have allowed for access to research findings by the technical community, allowed for application of technical data by the food industry, and helped to educate consumers on benefits and functions of fruits, vegetables and grains in their diet. Additionally, the gained knowledge was shared with researchers and consumers at numerous conferences and research conventions within the US and additionally generated an internation collaboration with the Agricultural University in Lima, Peru and Zamorano University, Honduras
Publications
- Manthey JA, Cesar TB, Jackson E, Mertens-Talcott SU. Pharmacokinetic Study of Nobiletin and Tangeretin in Rat Serum by HPLC-Electrospray Ionization-Mass Spectrometry. J Agric Food Chem. Jan 12;59(1):145-51. Epub 2010 Dec 6. PMID: 21133365
- Noratto G., Bertoldi M.C., Krenek, K, Talcott S.T.,. Stringheta P.C., Mertens-Talcott S.U. (corresponding author) Anticarcinogenic Effects of Polyphenolics from Mango (Mangifera indica) Varieties. J. Agric Food Chem., 58, 4104-4112, 2010
- Pacheco-Palencia, L., Mertens-Talcott, SU., Talcott, S.T. 2010. In-vitro Absorption and Antiproliferative Activities of Monomeric and Polymeric Anthocyanin Fractions from Acai Fruit (Euterpe oleracea Mart.). J. Agric. Food Chemistry, 119, 1071-1078
|
Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: Outputs for these projects were generated in several ways. Peer-reviewed manuscripts were generated in the areas of molecular biology, nutrition and food chemistry in reputable journals.For industry-sponsored projects, technical reports were generated for industry as well as non-technical publications in form of AgriLife Extension publications were generated for the two commodities mango and acai. These Agrilife publications were released nationally and internationally and generated great interest in the media. These written articles were released to the Associated Press as well as local and state media outlets. These outcomes have allowed for open access to research findings by the technical community, allowed for application of technical data by the food industry, and helped to educate consumers on benefits and functions of fruits, vegetables and grains in their diet. Additionally, the gained knowledge was shared with researchers and consumers at numerous conferences and research conventions within the US and additionally generated an internation collaboration with the Universidad Federale Vicosa, Brazil. PARTICIPANTS: Graduate students: Armando Del Follo, Gaby Angel, Emily Townsley; sal Bertucci, Postdoctoral researcher: Giuliana Noratto, Xiangrong Li; Undergraduate students: Teryin Wixon, Alex Bass, all contributed to the performance of the research projects. The Institute for Ob[provided a research platform in supported of the research. Funding was received from the NIH, AHA, and the National Mango board which funded the research performed on mangos. TARGET AUDIENCES: Students, communication with colleagues, industry partners, funding agencies (USDA, NIH, AHA and others). PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Results from research studies were communicated at differnt levels. Mango, fruit were evaluated in their anti-cancer activity in differnt types of cancer. Two of the varieties had exceptional activity, which was communicated to a mango industry board for their future planning of the cultivation of specific cultivars. Several in vitro studies were performed investigating the anti-inflammatory mechanisms of so-called superfruit. Findings from these studies were communicated to interested industry as well as research-colleagues and students and also are expected to result in peer-reviewed publications. Moreover, two human clinical trials in the areas of absorption of polyphenolics, inflammation and cancer are in preparation which generated fruitful interdisciplinary collaborations. Studies performed in the area of inflammation and whole grain will have a significant influence on the large number of obese individuals in the US, and also are expected to influence product development and research in this area. The study performed in the anti-cancer effects of botanicals should generate a follow-up study with clinical relevance.
Publications
- Li, X, Mertens-Talcott SU, Zhang S, Ball J, Safe S. MicroRNA-27a Indirectly Regulates Estrogen Receptor a Espression and Hormone-Responsiveness in MCF-7 Breast Cancer Cells. (2009) Mol Endocrinol. In press
- Chintharlapalli S, Papineni S, Abdelrahim M, Abudayyeh S, Wu F. Mertens-Talcott SU, Vanderlaag K, Cho, SD, Smith R, Safe S. Oncogenic MicroRNA-27a is a target for Anticancer Agent Methyl 2-Cyano3,11-dioxo-18Sb-olean-1,2-dien-30-oate in Colon Cancer Cells. Int J Cancer. 2009 Oct 15;125(8):1965-74.
- Pacheco-Palencia L., Mertens-Talcott, SU, Talcott, ST, In-vitro Absorption and Antiproliferative Activities of Monomeric and Polymeric , Anthocyanin Fractions from Acai Fruit (Euterpe oleracea Mart.), J Agric Food Chem, 2009,
|
|