Source: IOWA STATE UNIVERSITY submitted to NRP
SHEDDING AND SEROPREVALENCE OF COXIELLA BURNETTII AMONG IOWA RESIDENTS AND RUMINANTS LIVESTOCK
Sponsoring Institution
Cooperating Schools of Veterinary Medicine
Project Status
ACTIVE
Funding Source
STATE
Reporting Frequency
Annual
Accession No.
0218468
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2009
Project End Date
Jun 30, 2010
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
IOWA STATE UNIVERSITY
S. AND 16TH ELWOOD
AMES,IA 50011
Performing Department
Veterinary Medicine
Non Technical Summary
Coxiella burnetti is the causitive agent for the zoonotic disease called Q-fever. Ruminants act as a reservoir for the organism in the environment and call be a source of human infection via either the direct or aerosol route. Current data concerning the potential for exposure and shedding of the organism by ruminants in Iowa is not available. This project is designed to provide an improved understanding of the significance of ruminant and human C. burnetti infections in the state.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31139101100100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3910 - Cross-commodity research--multiple animal species;

Field Of Science
1100 - Bacteriology;
Goals / Objectives
The goal of this project is to better quantitate the prevalence of Coxiella burnetti in Iowa. There are three specific aims proposed in the project. The first specific aim will quantitate the herd-level seroprevalence and the shedding prevalence of C. burnetti in Iowa Dairy Cattle and Dairy Goat herds. The second specific aim will focus on quantitating the individual animal fecal shedding prevalence for beef cattle, meat goats and sheep of Iowa. The final specific aim will focus on establishing a baseline for human seroprevalence of blood-banked sera from healthy adult Iowans. These aims will allow us to better understand the overall picture of C. burnetti prevalenece and shedding in the state and will allow for additional studies looking at the ecology of the organism in the state.
Project Methods
Random sampling of bulk tanks from both dairy and dairy goat operations of the state will be utilized for specific aim 1. For the seroprevalence studies we will utilize an IFA assay that is specific for both Phase 1 and Phase 2 IgG responses. For specific aim 2 we will utilize a real-time PCR assay for detection of the organism in feces of livestock sampled at representative county fairs in the state. The final aim will utilize blood samples submitted to the Iowa Hygeinics Laboratory as a database of health adult Iowans. The samples will be subjected to an IFA specific for both Phase 1 and phase 2 responses.