Progress 10/01/08 to 09/30/13
Outputs
Target Audience: Target audiences for this work were the international scientific community concerned with perennial plant species as well as breeders who deal with fruit and nut tree crops. Outreach was performed through presentation of results of our HTP genotyping efforts at the Plant & Animal Genome conference in January 2013 and the International Plant Dormancy Symposium in November 2013. Changes/Problems: Transgenic approaches to manipulate the SVP clade genes in a hybrid poplar system have proven unreliable. Low recovery of transgenic lines and poor performance of putative transgenic explants suggests these genes are conferring a slow/no growth phenotype which inhibits their recovery from tissue culture. We are currently adapting high density marker discovery methodologies to the fine-mapping and association mapping of dormancy phenology traits in Prunus species where we can identify other genes with important regulatory roles in seasonal phenology. What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest? Results have been disseminated through oral presentations at international plant science meetings: Bielenberg DG, Rauh B, Wells CE. 2013. Genetic Basis of chilling requirement for bud break: genome-enabled insights from Prunus. Invited talk. Plant Dormancy 2013, 4th Quadrennial meeting of the International Plant Dormancy Conference, Nov. 4-9, 2013. Auckland, New Zealand. Rauh B, Fan S, Abbott AG, Gasic K, Okie WR, Bielenberg DG. 2013. Genotyping-by-sequencing for quantitative trait loci mapping of chilling requirement and bloom date in peach. Invited talk. Plant and Animal Genome XXI Conference, January 14-19, 2013. San Diego, California. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Transgenic approaches to manipulate the SVP clade genes in a hybrid poplar system have proven unreliable. Low recovery of transgenic lines and poor performance of putative transgenic explants suggests these genes are conferring a slow/no growth phenotype which inhibits their recovery from tissue culture. We have adapted high density marker discovery methodologies to the fine-mapping and association mapping of dormancy phenology traits in Prunus species where we can identify other genes with important regulatory roles in seasonal phenology. These methods have proven highly successful and we have been able to narrow down the candidate genes for regulation of seasonal phenology.
Publications
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Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: Transgenic approaches to manipulate the SVP clade genes in a hybrid poplar system have proven unreliable. Low recovery of transgenic lines and poor performance of putative transgenic explants suggests these genes are conferring a slow/no growth phenotype which inhibits their recovery from tissue culture. We are currently adapting high density marker discovery methodologies to the fine-mapping and association mapping of dormancy phenology traits in Prunus species where we can identify other genes with important regulatory roles in seasonal phenology. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
The long term objective of this work is to discover regulators of bud dormancy induction in trees for rapid improvement of horticultural and forestry species. Targeted manipulation of winter dormancy phenology has the potential to allow adaptation of important perennial crops to future climate change and improve prediction of tree phenology on an annual basis.
Publications
- Jimenez S, Reighard GL, and Bielenberg DG. 2012. Peach dormancy associated MADS-box gene expression during natural chilling accumulation. Acta Horticulturae 962: 161-167.
- Rauh B, Fan S, Abbott AG, Gasic K, Okie WR, Bielenberg DG. 2012. Genotyping-by-sequencing for quantitative trait loci mapping of chilling requirement and bloom date in peach. Poster presentation. 6th Rosaceae Genomes Conference, September 30-October 4, 2012. Trento, Italy.
- Zhebentyayeva T, Fan S, Olukolu B, Barakat A, Hughes-Murphree S, Leida C, Badenes M, Bielenberg DG, Reighard GL, Kole C, Okie WR, Abbott AG. 2012. Chilling requirement and bloom date in peach: genetic and genomic approaches for deciphering a complex gene network. Oral presentation. Plant and Animal Genome XX Conference, January 13-18, 2012. San Diego, California.
- Wells CE, Feltus FA, Reighard GL, Bielenberg DG. 2012. Quantitative differential gene expression with NGS to uncover regulators of short-day induced growth cessation in peach. Poster presentation. Plant and Animal Genome XX Conference, January 13-18, 2012. San Diego, California.
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Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: We have produced two transgenic lines for the functional evaluation of the poplar SVP homologs. We have analyzed the expression of the SVP homologues from June through December in order to assign putative homologous function of the poplar SVPs to the peach DAM genes from the EVG locus. These genes appear to have a very different regulatory pattern than the peach DAM homologs. One M.S. graduate student has defended his thesis on this work, completed his dissertation and graduated. He is now pursuing a Ph.D. from the University of Pennsylvania. This student was supported on a USDA-AFRI Seed Grant award to DG Bielenberg. A second graduate student was recruited on a teaching assistantship funded by the Biological Sciences Department. This student failed to make adequate progress on his degree and was terminated from the project. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
The long term objective of this work is to discover regulators of bud dormancy induction in trees for rapid improvement of horticultural and forestry species. Targeted manipulation of winter dormancy phenology has the potential to allow adaptation of important perennial crops to future climate change and for prediction of tree phenology on an annual basis.
Publications
- Bielenberg DG. 2011. Knowing when not to grow. New Phytologist 189(1): 3-5.
- Wells CE, Jimenez Tarodo S, Verde I, Sosinski B, and Bielenberg DG. 2012. A genome-wide analysis of MADS box genes in peach [Prunus persica (L.) Batsch]. Poster presentation. Plant and Animal Genome XX Conference, January 13-18, 2012. San Diego, California.
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: We are currently attempting to produce transgenic lines for the functional evaluation of the poplar SVP homologs. We have analyzed the expression of the SVP homologues from June through December in order to assign putative homologous function of the poplar SVPs to the peach DAM genes from the EVG locus. One M.S. graduate student has defended his thesis on this work and is currently revising his thesis. A second M.S. student is currently in progress on the project and it being supported by a graduate teaching assistantship. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
The long term objective of this work is to discover regulators of bud dormancy induction in trees for rapid improvement of biomass and agricultural crop productivity by extending fall growth periods where winter injury is not a threat. The current stage of the work is the production of putative transgenic plants to assess their dormancy phenotype.
Publications
- No publications reported this period
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Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: We have cloned and isolated the hybrid poplar SVP homologs. We used these sequences to construct plant overexpression vectors to be introduced into hybrid poplar trees. We are currently producing putative transgenic trees for functional evaluation. One M.S. degree candidate was recruited for the project and is being funded on a Graduate Teaching Assistantship. One NSF proposal was submitted in aid of funding the proposed work. PARTICIPANTS: Dr. Douglas G. Bielenberg is the PI of this project and is responsible for submitting grants for the funding of the project, advising students, organizing and developing experimental plans, analysis of the results and dissemination of the results in peer-reviewed journals. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
The long term objective of this work is to discover regulators of bud dormancy induction in trees for rapid improvement of biomass and agricultural crop productivity by extending fall growth periods where winter injury is not a threat. The current stage of the work is the production of putative transgenic plants to assess their dormancy phenotype.
Publications
- Jimenez S, Lawton-Rauh AL, Reighard GL, Abbott AG, Bielenberg DG. 2009. Phylogenetic analysis and molecular evolution of the dormancy associated MADS-box genes from peach. BMC Plant Biology 9(81): doi:10.1186/1471-2229-1189-1181.
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