Progress 10/01/08 to 09/30/14
Outputs
Target Audience: Entomologists in Oklahoma, U.S., and Worldwide Oklahoma, U.S., and Worldwide Grains and Feed Industries Oklahoma, U.S., and Worldwide Grain Milling and Processing Industries Oklahoma, U.S., and Worldwide Grains Storage System Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? 1-Altogether 3 M.S. students and 1 PhD student were trained. 2-Graduate students and the PI attended several national and international meetings where research data were presented. How have the results been disseminated to communities of interest? Results have been disseminated to different communities of interest through poster presentations, talks at industry and professional meetings, industry publications, and publications in peer-reviewed journals. What do you plan to do during the next reporting period to accomplish the goals? Continue to research the biology and integrated management of stored-product psocid, coleopteran, and lepidopteran pests.
Impacts
What was accomplished under these goals?
1-Data that improve our understanding of the biology and economic impact of stored-product psocids were obtained and published. 2-New data to improve management of coleopteran pests of stored products were obtained and published.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2014
Citation:
Gautam, S.G., G. P. Opit, D. Margosan, D. Hoffmann, J. S. Tebbets, and S. Walse. Comparative egg morphology and chorionic ultrastructure of key stored-product insect pests. Annals of Entomological Society of America (in press).
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Wang, Y.J., Z.H. Li, S.F. Zhang, Z. Varad�nov�, F. Jiang, Z. Ku?erov�, V. Stejskal, G. Opit, Y. Cao, and F.J. Li. 2014. DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae). Bulletin of Entomological Research, available on CJO2014. doi:10.1017/S0007485314000224.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Nwaubani, S. I., G. P. Opit, G. O. Otitodun, and M. A. Adesida. 2014. Efficacy of Two Nigeria-Derived Diatomaceous Earths against Sitophilus oryzae (Coleoptera: Curculionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) on wheat. Journal of Stored Products Research 59: 9-16.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Gautam, S.G., G.P. Opit, D. Margosan, J.S. Tebbets, and S. Walse. 2014. Egg morphology of key stored-product insect pests of the United States. Annals of Entomological Society of America 107: 1-10. (FORUM ARTICLE).
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Ku?erov�, Z., W. Wakil, M. Yasin, Q. Yang, Z. Li, J. Hrom�dkov�, I. Kalinovic, G. Opit, and C. Lienhard. 2014. Morphological and molecular characterization of a Pakistan strain of the stored-product pest Liposcelis paeta (Insecta: Psocodea: Liposcelididae) with observations on the variability of the head surface sculpture in liposcelidids. Journal of Stored Products Research 57: 12-23.
- Type:
Other
Status:
Published
Year Published:
2014
Citation:
Opit, G. P. 2014. Coping with Phosphine Resistance. Milling Journal (Third Quarter): 18-22.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Opit, G. P., and S. Bajracharya. 2014. Strong Phosphine Resistance in Stored Grain Insects in the U.S. and Implications for Management? International Miller (First Quarter): 17-19.
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Progress 10/01/12 to 09/30/13
Outputs
Target Audience: Entomologists in Oklahoma, U.S., and Worldwide Oklahoma, U.S., and Worldwide Grains and Feed Industries Oklahoma, U.S., and Worldwide Grain Milling and Processing Industries Oklahoma, U.S., and Worldwide Grain Storage Systems Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? 1) Altogether 3 M.S. students and 1 PhD student were trained. 2) Graduate students and the PI attended numerous national and international meetings where research data were presented. How have the results been disseminated to communities of interest? Results have been disseminated to different communities of interest through poster presentations, talks at industry and professional meetings, industry publications, and publications in peer-reviewed journals. What do you plan to do during the next reporting period to accomplish the goals? Continue to research the biology and integrated management of stored-product psocid, coleopteran, and lepidopteran pests.
Impacts
What was accomplished under these goals?
1) Data that improve our understanding of the biology and economic impact of stored-product psocids was obtained and published. 2) New data to improve management of coleopteran pests of stored products was obtained and published.
Publications
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Oppert, B., T.D. Morgan, T.W. Phillips, M.J. Aikins, G.P. Opit, R.N.C. Guedes, K. Hoon, Y. Sun, G. Meridith, K. Bramlett, N.S. Hernandez, B. Sanderson, M. Taylor, D. Dhingra, and B. Blakey. 2013. Using RNA-Seq to understand phosphine resistance in two stored-product beetles. Poster: 14th Annual Advances in Genome Biology and Technology (AGBT) Meeting. Marco Island, FL. February 20-23, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G.P. 2013. Food security in sub-Saharan Africa. Strong phosphine resistance in stored grain insects in Oklahoma and implications for management. Talk: Oklahoma State University Department of Entomology and Plant Pathology (EPP) Spring 2013 Seminar Series. Stillwater, OK.
January 9, 2013.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Bajracharya, N. S., G. P. Opit, J. Talley, and C. L. Jones. 2013. Efficacy of Spinosad and a Combination of Chlorpyrifos-Methyl and Deltamethrin Against Phosphine-Resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) on Wheat. Journal of Economic Entomology 106: 2208-2215.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Yang, Q., S. Zhao, Z. Ku?erov�, G. Opit, Y. Cao, V. Stejskal, and Z. Li. 2013. Rapid molecular diagnosis of the stored-product psocid Liposcelis corrodens (Psocodea: Liposcelididae): species-specific PCR primers of 16S rDNA and COI. Journal of Stored Products Research 54: 1-7.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Yang, Q., S. Zhao, Z. Ku?erov�, V. Stejskal, G. Opit, M. Qin, Y. Cao, F. Li, Z. Li. 2013. Validation of the 16S rDNA and COI DNA Barcoding Technique for Rapid Molecular Identification of Stored Product Psocids (Insecta: Psocodea: Liposcelididae). Journal of Economic Entomology 106: 419-425.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Gautam, S.G., G.P. Opit, K.L. Giles, and B. Adam. 2013. Weight loss and germination failure caused by psocids in different wheat varieties. Journal of Economic Entomology 106: 491-498.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2013
Citation:
Phosphine resistance in stored-product insect pests: Management and fitness cost
- Type:
Other
Status:
Published
Year Published:
2013
Citation:
Opit, G. P., and S. Bajracharya. Can Phosphine-Resistant Lesser Grain Borers and Red Flour Beetles Still Thrive in the Absence of Phosphine Use? Fumigants & Pheromones Issue 108 (Fall 2013): 7.
- Type:
Other
Status:
Published
Year Published:
2013
Citation:
Opit, G., and T. Phillips. 2013. Strong Phosphine Resistance Found in U.S. Stored Grain Insects. Feed and Grain, June/July 2013 Issue: 18-23 (Figure 4): 18-23.
- Type:
Other
Status:
Published
Year Published:
2013
Citation:
Opit, G.P., and S. Bajracharya. 2013. Phosphine-Resistant Stored-Product Insect Pests can be Destroyed! Fumigants & Pheromones Issue 106 (Spring 2013): 4-5.
- Type:
Other
Status:
Published
Year Published:
2013
Citation:
Gautam, S., G.P. Opit, and S. Walse. 2013. Insect Eggs why are some stored product insect eggs difficult to kill using fumigants? Fumigants & Pheromones Issue 105 (Winter 2013): 7.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G.P. and N. S. Bajracharya. 2013. Effects of phosphine resistance on fitness in Rhyzopertha dominica and Tribolium castaneum from Oklahoma. The 6st Annual Meeting of the Entomological Society of America. Austin, TX. November 10-13, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G.P. 2013. Phosphine resistance and its management in the United States. Annual Meeting of the Entomological Society of Canada. Guelph, ON, Canada. October 20-23, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Gautam, S.G., S. Walse, J.S. Tebbets, and G. P. Opit. 2013. Efficacy of propylene oxide in combination with carbon dioxide against eggs of stored-product insect pests. Methyl Bromide Alternatives Outreach Meeting. San Diego, CA. November 4-6, 2013.
- Type:
Other
Status:
Awaiting Publication
Year Published:
2013
Citation:
Konemann, C., and G.P. Opit. 2013. Phosphine Resistance in Cryptolestes ferrugineus (Coleoptera: Laemophloeidea) From Stored Wheat in Oklahoma. The 9th Conference of the IOBC/WPRS (OILB/SROP) Working Group on Integrated Protection of Stored Products . Bordeaux, France. July 1-4, 2013.
- Type:
Other
Status:
Awaiting Publication
Year Published:
2013
Citation:
Opit, G.P., S.G. Gautam, S. Walse, and D. Margosan. 2013. Influence of Egg Morphology on Ovicidal Efficacy of Fumigants. The 9th Conference of the IOBC/WPRS (OILB/SROP) Working Group on Integrated Protection of Stored Products . Bordeaux, France. July 1-4, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G. P. 2013. Phosphine Resistance in Rhyzopertha dominica and Tribolium castaneum: Resistance Management. USDA ARS San Joaquin Valley Agricultural Sciences Center. Parlier, CA. June 7, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G. P. 2013. Detection and Levels of Phosphine Resistance in Indianmeal Moth, Red Flour Beetle, and Dried Fruit Beetle. Almond Board of California Almond Food Quality and Safety Committee. Modesto, CA. June 4, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G. P. 2013. Phosphine Resistance in Major Postharvest Insects from Stored Wheat in Oklahoma USA. Nigerian Stored Products Research Institute, Lagos Research Center. Lagos, Nigeria. May 16, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G. P. 2013. Assessment of the Fitness Cost of Phosphine Resistance in Rhyzopertha dominica and Tribolium castaneum. Nigerian Stored Products Research Institute, Lagos Research Center.
Lagos, Nigeria. May 21, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G. P. 2013. Influence of Egg morphology on Ovicidal Efficacy of Fumigants. Nigerian Stored Products Research Institute, Lagos Research Center. Lagos, Nigeria. May 23, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Opit, G.P. 2013. Strong phosphine resistance in stored grain insects in Oklahoma and implications for management. Oklahoma State University, Department of Entomology and Plant Pathology (EPP) Spring 2013 Seminar Series. Stillwater, OK. January 9, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Bajracharya N., G.P. Opit, J. Talley, and C.L. Jones. 2013. Fitness Effects of Phosphine Resistance Determined by Measurement of Developmental Rates of Resistant and Susceptible Populations of
Rhyzopertha dominica and Tribolium castaneum. Poster: 61st Annual Meeting of the Entomological Society of America. Austin, TX. November 10-13, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Bajracharya N., G.P. Opit, J. Talley, and C.L. Jones. 2013. Comparing Effectiveness of Three Traps Used to Monitor Tribolium castaneum (Coleoptera: Tenebrionidae). Talk: 61st Annual Meeting of the Entomological Society of America. Austin, TX. November 10-13, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Gautam, S.G., G.P. Opit, J.S. Tebbets, D. Margosan, D. Hoffman, and S. Walse. 2013. Possible relationship between egg morphology of stored-product insect eggs and fumigant efficacy. Talk: 61st Annual Meeting of the Entomological Society of America. Austin, TX. November 10-13, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Gautam, S.G., G.P. Opit, J.S. Tebbets, and S. Walse. 2013. Efficacy of propylene oxide against eggs of four key California-based stored-product insect pests. Poster: 61st Annual Meeting of the Entomological Society of America. Austin, TX. November 10-13, 2013.
- Type:
Other
Status:
Awaiting Publication
Year Published:
2013
Citation:
Opit, G.P. 2013. Strong Phosphine Resistance in Stored Grain Insects in the U.S. and Implications for Management. International Association of Operative Millers (IAOM) 2013 Annual Conference Niagara Falls, Canada. April 29 May 3, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Gautam, S.G., G.P. Opit, J.S. Tebbets, and S. Walse. 2013. Efficacy of propylene oxide against eggs of four key California stored-product insect pests. Poster: 10th Annual Graduate Research in the Biological Sciences Symposium, BMBGSA, OSU. Stillwater, OK. September 19-20, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Bajracharya N., G.P. Opit, J. Talley, and C.L. Jones. 2013. Fitness Effects of Phosphine Resistance Determined by Measurement of Developmental Rates of Resistant and Susceptible Populations of Rhyzopertha dominica and Tribolium castaneum. Poster: 10th Annual Graduate Research in the Biological Sciences Symposium, BMBGSA, OSU. Stillwater, OK. September 19-20, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Bajracharya N., G.P. Opit, J. Talley, and C.L. Jones. 2013. Assessment of the fitness cost of phosphine resistance in Rhyzopertha dominica and Tribolium castaneum through population growth rate studies. Poster: 61st Annual Meeting of the Southwestern Branch of the Entomological Society of America. Las Cruces, NM. February 25-28, 2013.
- Type:
Other
Status:
Other
Year Published:
2013
Citation:
Gautam, S.G., G.P. Opit, J.S. Tebbets, and S. Walse. 2013. Efficacy of propylene oxide against eggs of six species of stored-product insects under vacuum. Poster: 61st Annual Meeting of the Southwestern Branch of the Entomological Society of America. Las Cruces, NM. February 25-28,
2013.
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Progress 10/01/11 to 09/30/12
Outputs
OUTPUTS: Research on phosphine (PH3 or hydrogen phosphide) resistance of lesser grain borer (Rhyzopertha dominica) and red flour beetle (Tribolium castaneum) found strong resistance that was 1,519 and 119 times, respectively, higher than that in susceptible counterparts of these species. Data were presented at three international entomology conferences, the North Central Branch of Entomological Society of America (ESA) meeting, and have been published in a peer-reviewed journal. Given the strong PH3 resistance found above, research on PH3 resistance management was conducted to find practical ways to kill highly PH3-resistant insects in grain storage facilities. Storcide II and Sensat (Spinosad) were found to be highly effective against PH3-resistant insects. Data were presented at two ESA meetings. To gain understanding on whether PH3 resistance management programs can be effective if implemented, research to determine the fitness cost or benefit of possessing PH3 resistance genes in R. dominica and T. castaneum was conducted. Data show that in the Oklahoma strains of R. dominica and T. castaneum, there is a fitness cost and benefit, respectively, of possessing resistance genes. Research on understanding the relationship between respiratory structures of stored-product insect eggs and their tolerance or susceptibility to fumigants used for their control was conducted. Generally, data indicate that eggs which have a large number of aeropyles and a micropyle are more susceptible to fumigants. Data from this work were presented at one international entomology conference and one ESA meeting. In addition, data have been submitted to a trade journal for publication. Stored-product psocids are similar in appearance and difficult to identify using morphological characters, therefore, research on their molecular identification of was conducted. Data indicate that nucleotide sequences based on the CO1 gene can be used for rapid and accurate identification of Lepinotus reticulatus. Data have been published in a peer-reviewed journal. Research to compare the effectiveness of three traps for their ability to effectively monitor T. castaneum populations in facilities containing grain and grain products was conducted. The Dome trap caught the highest number of T. castaneum and was twice as effective as the ClimbUP BG and Torios traps. Data from this work were presented at the annual ESA meeting. Five competitive grant proposals were written but not funded by the United States Department of Agriculture /Foreign Agricultural Service, Oklahoma Wheat Commission, USDA-APHIS FY12 Farm Bill section 10201, USDA-NIFA Southern Region Integrated Pest Management Competitive Grants Program, and USDA-NIFA North Central Region Integrated Pest Management Competitive Grants Program. Funding requested was $40,000, $32,543, $70,822, $136,781, and $114,004, respectively. Two industry proposals were submitted to Sergeants Pet Care Products and one to Bayer Crop Science. These were funded and amounts awarded were $3,600 and $5,000 and $21,870, respectively, for a total of $30,470. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Oklahoma grain industry United States grain industry International grain industry Entomologists worldwide Local and international grain and food processing industries PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Eight invited oral presentations were delivered. At least 30 stored-product-related inquiries were received and responded to by phone and email. Three industry proposals were funded by Sergeants Pet Care Products and Bayer Crop Science. Total funding from these sources was $30,470. 45 stakeholders in Ghana were trained at a train-the-trainer workshop on reducing grain post-harvest losses in Ghana. In response to a request from Dow AgroSciences, LLC, a support letter was written to EPA in support of ProFume (sulfuryl fluoride).
Publications
- Opit, G.P., F.H. Arthur, J.E. Throne, and M.E. Payton. 2012. Susceptibility of stored-product psocids to aerosol insecticides. Journal of Insect Science 12:139 (1-14). Available online: http://www.insectscience.org/12.139.
- Gautam, S.G., G.P. Opit, and K.L. Giles. 2012. Effects of Constant Temperatures on Reproductive Parameters of the Psocid Liposcelis rufa (Psocoptera: Liposcelididae). Entomology, Ornithology, and Herpetology S1:002. doi:10.4172/2161-0983.S1-002.
- Arif, M., F.M. Ochoa Corona, G.P. Opit, Z.H. Li, Z. Kučerova, V. Stejskal, and Q.Q. Yang. 2012. PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae). Journal of Stored Product Research 49: 184-188.
- Opit, G.P., P.J. Collins, and G.J. Daglish. 2012. Resistance Management. In T.W. Phillips, D.W. Hagstrum, and G.W. Cuperus (eds.), Stored Product Protection. Kansas State University. (Book chapter).
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Progress 10/01/10 to 09/30/11
Outputs
OUTPUTS: Research on understanding the relationship between respiratory mechanisms of stored-product insect eggs and efficacy of fumigants used for their control was initiated. Data from this work has already resulted in 2 presentations given at professional meetings. Research on phosphine resistance management was initiated. Experiments on efficacy of Nigeria-derived diatomaceous earths (DE) and mixtures of DE and botanical insecticides from Nigeria for the control of stored product insect pests were completed. Data are currently being analyzed for presentations at professional meetings and publication in a peer-reviewed journal. Research on molecular identification of stored-product psocids is in progress and this has already resulted in 2 peer-reviewed publications and 1 submitted to a peer-reviewed journal for publication. Experiments on the effects of temperature and relative humidity on population growth and development of Liposcelis fusciceps were completed. Data are currently being analyzed for presentations at professional meetings and publication in a peer-reviewed journal. Experiments on the effects of temperature on reproductive parameters of Liposcelis rufa have been completed and a manuscript on this has been written and submitted to Entomology, Ornithology, and Herpetology. This work also resulted in 2 presentations at professional meetings. Experiments on the effects of temperature and relative humidity on population growth and development of Liposcelis pearmani were completed and a manuscript on this was published. Data from experiments to determine population growth of Liposcelis species (Psocoptera: Liposcelididae) on varieties of wheat grown in Oklahoma and weight losses caused by psocids in these varieties are being analyzed for presentations and publication. This work has already resulted in 1 presentation at a professional meeting. Data from experiments to determine immigration of psocids into steel bins containing wheat are being analyzed for presentations and publications. Four proposals were written to and funded by the United States Department of Agriculture /Foreign Agricultural Service (USDA/FAS), Oklahoma Agriculture Enhancement and Diversification Program, USDA/FAS Borlaug Fellowship Program, and USDA-ARS San Joaquin Valley Agricultural Sciences Center (SJVASC). Total funding from these was $123,744.00. As a result of these and earlier funding, I made 2 trips to Ghana to implement a Technical Assistance Program on Reducing Post-harvest Losses in Ghana; 1 to Nigeria on a follow-up visit for a Borlaug Fellow who worked under my supervision in my laboratory in 2010; I received and supervised another Borlaug Fellow from Nigeria in my laboratory for 3 months; and I recruited 1 Ph. D. student and 1 M.S. student. I also wrote 4 industry proposals to Douglas Products and Packaging Co., Bayer Crop Science, Sergeants Pet Care Products, and Susan McKnight, Inc. All these got funded for the total amount of $31,795.00.Two proposals totaling $478,227.00 were declined and one for $43,846.00 is pending. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Oklahoma grain industry United States grain industry International grain industry Entomologists worldwide Local and international grain and food processing industries PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
I received and responded to at least 40 stored-product-related inquiries by phone and email. Four proposals were funded by the United States Department of Agriculture/Foreign Agricultural Service (USDA/FAS), Oklahoma Agriculture Enhancement and Diversification Program, USDA/FAS Borlaug Fellowship Program, and USDA-ARS San Joaquin Valley Agricultural Sciences Center (SJVASC). Total funding from these was $123,744.00. Four industry proposals were funded by Douglas Products and Packaging Co., Bayer Crop Science, Sergeants Pet Care Products, and Susan McKnight, Inc. Total funding from these was $31,795.00. Over 160 stakeholders attended my oral presentations at the Minnesota Pest Management Conferences - Food Plant Pest Management Conference, Minneapolis, MN and the U.S. State Department African Food Security Fellows Seminars. Over 180 stakeholders in Ghana attended 4 workshops in which I gave several oral presentations on reducing grain post-harvest losses in Ghana. I received invitations for 8 oral presentations which I delivered. Dow AgroSciences, LLC requested me to write a letter to EPA in support of ProFume because of my research on the use of heat to control stored-product insect pests and phosphine resistance in these pests.
Publications
- Opit, G.P., F.H. Arthur, E.L. Bonjour, C.L. Jones, and T.W. Phillips. 2011. Efficacy of heat treatment for disinfestation of concrete grain silos. Journal of Economic Entomology 104: 1415-1422.
- Aminatou, B.A., S.G. Gautam, G.P. Opit, J. Talley, and K. Shakya. 2011. Population growth and development of Liposcelis pearmani (Psocoptera: Liposcelididae) at constant temperatures and relative humidities. Environmental Entomology 40: 788-796.
- Chen, H., G. P. Opit, P. Sheng, and H. Zhang. 2011. Maternal and progeny quality of Habrobracon hebetor Say (Hymenoptera: Braconidae) after cold storage. Biological Control 58: 255-261.
- Yang, Q., Z. Kucerova, Z. Li, I. Kalinovic, V. Stejskal, G. Opit, and Y. Cao. 2012. Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes. Journal of Stored Product Research 48: 120-125
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Progress 10/01/09 to 09/30/10
Outputs
OUTPUTS: 1.Experiments on the effects of temperature and relative humidity on population growth and development of L. rufa were completed. This work resulted in 1 peer reviewed publication (Journal of Economic Entomology 103: 1920-1928) and 2 presentations at professional meetings; one of the presentations won first prize. 2.Experiments on the effects of temperature on reproductive parameters of L. rufa have been completed and a manuscript on this is in preparation. This work resulted in 1 presentation at a professional meeting. 3.Experiments on the effects of temperature and relative humidity on population growth and development of L. pearmani were completed and a manuscript on this is in preparation. This work resulted in 2 presentations at professional meetings. 4.Experiments on population growth of Liposcelis species (Psocoptera: Liposcelididae) on varieties of wheat grown in Oklahoma have been completed and a manuscript on this is in preparation. This work resulted in 1 presentation at a professional meeting; this presentation won second prize. 5.Experiments on efficacy of ozone against major grain pests in stored wheat were completed. This work resulted in 1 peer reviewed publication (Journal of Economic Entomology [in press]). 6.Experiments to determine immigration of psocids into steel bins containing wheat were completed. Data are being analyzed for presentations and publications. 7.Four proposals were written to and funded by the United States Department of Agriculture - Foreign Agricultural Service (USDA-FAS). As a result, I made 2 trips to Nigeria to implement a Technical Assistance Program on Grain Storage and Handling in Nigeria. I also made 1 trip to Ghana on a Technical Assistance Program to Reduce Post-Harvest Grain Losses in Ghana. 8.Three proposals were submitted to and funded by industry stakeholders. These were related to experiments on integrated management of coleopteran and lepidopteran stored-product pests. Two of the projects are still in progress and 1 has been completed and a report written and submitted to the funding source. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: 1-Oklahoma grain industry 2-United States grain industry 3-International grain industry 4-Entomologists worldwide 5-Local and international grain and food processing industries PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
1.I received and responded to at least 80 stored-product related inquiries by phone and email. 2.Four proposals were funded by the United States Department of Agriculture - Foreign Agricultural Service (USDA-FAS). I received $196,915.00 for these proposals during this period. 3.Three industry proposals were funded for stored-product pest management research (DuPont, Douglas Products and Packaging Company, and Sergeants Pet Care Products Company). I received $44,390.30 in industry funding for these proposals during this period. 4.Over 250 stakeholders have attended my oral presentations at the following meetings: Oklahoma State University - Stored Product Research and Education Center (OSU-SPREC) Fumigation Practical; Kansas State Research and Extension IPM and Pesticide Safety Education Workshop; and the Missouri Fumigation Certification and Recertification Workshop. 5.Over 120 stakeholders in Nigeria attended two workshops in which I gave several oral presentations on proper grain management. 6.I received invitations for 6 oral presentations which I delivered.
Publications
- Gautam, S.G., G.P. Opit, and K.L. Giles. 2010. Population Growth and Development of the Psocid Liposcelis rufa (Psocoptera: Liposcelididae) at Constant Temperatures and Relative Humidities. Journal of Economic Entomology 103: 1920-1928.
- Opit, G.P., J.E. Throne, and M.E. Payton. 2010. Reproductive Parameters of the Parthenogenetic Psocid Lepinotus reticulatus (Psocoptera: Trogiidae) at Constant Temperatures. Environmental Entomology 39: 1004-1011.
- Athanassiou, C.G., G.P. Opit, and J.E. Throne. 2010. Influence of Commodity Type, Percentage of Cracked Kernels, and Wheat Class on Population Growth of Stored-Product Psocids (Psocoptera: Liposcelididae). Journal of Economic Entomology 103: 985-990.
- Bonjour, E.L., G.P. Opit, J. Hardin, C.L. Jones, M.E. Payton, and R.L. Beeby. 2011. Efficacy of ozone against major grain pests in stored wheat. Journal of Economic Entomology (in press).
- Zhi-Hong, L., Z. Kucerova, S. Zhao, V. Stejskal, G. Opit, and M. Qin. 2011. Comparison of morphological and molecular identification of Liposcelis bostrychophila geographical populations (Psocoptera: Liposcelididae). Journal of Stored Product Research (in press).
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Progress 10/01/08 to 09/30/09
Outputs
OUTPUTS: 1.Two psocid species, namely, Liposcelis rufa and L. pearmani, which had previously not been documented as associated with grain storages and grain processing facilities in the United States were collected from a feed mill and steel bins containing wheat, respectively, and identified. 2.Experiments on the effect of temperature and relative humidity (RH) on population growth and development of L. rufa have been completed and a manuscript is being written. Presentations on this topic: A) S. Gautam. 2009. "Population Growth of the Psocid Liposcelis rufa Broadhead (Psocoptera: Liposcelididae) at Constant Temperatures and Relative Humidities". OSU Graduate Student Research Symposium; B) Gautam et al. 2009. "Population Growth of the Psocid Liposcelis rufa Broadhead (Psocoptera: Liposcelididae) at Constant Temperatures and Relative Humidities". Annual meeting of the Entomological Society of America (ESA). 3.Experiments to determine the effects of psocid predation by stored-product beetle pests and implications for pest management were conducted. Presentation on this topic: G. Opit & E. Bojour. 2009. "Effect of Interaction Between Tribolium confusum Duval (Coleoptera: Tenebrionidae) and Liposcelis decolor (Pearman) (Psocoptera: Liposcelididae)". Annual Meeting of the Southwestern Branch of the ESA. 4.User-friendly sampling protocols for stored-product psocids in grain storages have been developed. Data collected from steel bins containing wheat were used to write three manuscripts which have been published in the Journal of Economic Entomology. 5.Sampling studies conducted in grain storages, product warehouses, and grain processing facilities have provided some data that lays a foundation for the development of appropriate methods for stored-product psocid management. Presentations on this topic: A) G. Opit & E. Bojour. 2008. "Seasonal Distribution of Stored-Product Psocids". Annual meeting of the ESA; B) Opit et al. 2009. "Seasonal Abundance of Psocids in an Animal Feed Warehouse". IOBC-WPRS; C) Opit et al. 2009. "Seasonal Abundance and Distribution of Psocids in a Feed Mill". Annual meeting of the ESA. 6.Experiments have been conducted on the biology, ecology, behavior, and appropriate management of coleopteran and lepidopteran stored-product pests. This work was funded by industry money. Altogether 6 research projects were conducted and reports were written for the sponsors. 7.Renovation of the SPREC laboratory at a cost of $165,000.00 received as a grant from the Office of the Vice President for Research at OSU is nearing completion. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: 1-Oklahoma grain industry 2-United States grain industry 3-International grain industry 4-Entomologists worldwide 5-Local and international grain and food processing industries PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
1.I have received and responded to at least 90 stored-product related inquiries by phone and email. 2. Six industry stakeholders have requested and gotten assistance with stored-product pest management research (Birdola Products, Nonnis Food Co. Inc., Trece Inc., Dupont, GrainPro, and Bayer Crop Science). I have received $25,450.00 in industry funding during this period. 3. I have given talks to over 350 stakeholders at the following meetings: Spring Grain Elevator workshops, Fumigation Workshops, Food Industry Sanitation Auditors Seminar, and the Missouri Fumigation Certification and Recertification Workshop. 4. I have received a USDA-FAS grant for $6,677.00 to work on assessment of grain storage and handling practices in Nigeria. The second phase of funding for this project worth $200,000.00 has been awarded to our three-man team that completed the assessment. 5. Local and international collaborators have teamed up with me to write grants worth over $1.5 million. These grants are currently pending. 6. The Office of the Vice President for Research at OSU has awarded me a grant of $165,000.00 for renovation of the SPREC laboratory. The lab will be used for phosphine toxicity and other stored product research studies.
Publications
- Guedes, R.N. C., J.F. Campbell, F.H. Arthur, K.Y. Zhu, G.P. Opit, and J.E. Throne. 2008. Acute lethal and behavioral sublethal responses of two stored-product psocids to surface insecticides. Pest Management Science 64: 1314-1322.
- Opit, G.P., J.E. Throne, and P.W. Flinn. 2009. Sampling plans for the psocids Liposcelis entomophila and Liposcelis decolor (Psocoptera: Liposcelididae) in steel bins containing wheat. Journal of Economic Entomology 102: 1714-1722.
- Flinn, P. W., G. P. Opit, and J. E. Throne. 2009. Predicting stored grain insect population densities using an electronic probe trap. Journal of Economic Entomology 102: 1696-1704.
- Athanassiou, C.G., F.H. Arthur, G.P. Opit, and J.E. Throne. 2009. Insecticidal effect of diatomaceous earth against three species of stored-product psocids on maize, rice, and wheat. Journal of Economic Entomology 102: 1673-1680.
- Opit, G.P., J.E. Throne, and P. W. Flinn. 2009. Evaluation of five sampling methods for Liposcelis entomophila (Enderlein) and L. decolor (Pearman) (Psocoptera: Liposcelididae) in steel bins containing wheat. Journal of Economic Entomology 102: 1377-1382.
- Opit, G.P., J.E. Throne, and P. W. Flinn. 2009. Temporospatial distribution of the psocids Liposcelis entomophila (Enderlein) and L. decolor (Pearman) (Psocoptera: Liposcelididae) in steel bins containing wheat. Journal of Economic Entomology 102: 1369-1376.
- Opit, G.P. and J.E. Throne. 2009. Population growth and development of the psocid Liposcelis brunnea Motschulsky (Psocoptera: Liposcelididae) at constant temperatures and relative humidities. Journal of Economic Entomology 102: 1360-1368.
- Opit, G.P., J. Perret, K. Holt, J.R. Nechols, D.C. Margolies, and K.A. Williams. 2009. Comparing chemical and biological control strategies for twospotted spider mites(Acari: Tetranychidae) in commercial greenhouse production of bedding plants. Journal of Economic Entomology 102: 336-346.
- Opit, G.P. and J.E. Throne. 2008. Effects of diet on population growth of the psocids Lepinotus reticulatus and Liposcelis entomophila. Journal of Economic Entomology 101: 616-622.
- Opit, G.P. and J.E. Throne. 2008. Population growth and development of the psocid Lepinotus reticulatus at constant temperatures and relative humidities. Journal of Economic Entomology 101: 605-615.
- Guedes, R.N.C., K.Y. Zhu, G.P. Opit, and J.E. Throne. 2008. Differential heat shock tolerance and expression of heat-inducible proteins in two stored-product psocids. Journal of Economic Entomology 101: 1974-1982.
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