Source: IQF DREAM, LLC submitted to NRP
IMPROVED METHOD OF PROCESSING PAPAYAS FOR FOOD SAFETY AND QUALITY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0215678
Grant No.
2008-33610-19549
Cumulative Award Amt.
$350,000.00
Proposal No.
2008-02440
Multistate No.
(N/A)
Project Start Date
Sep 1, 2008
Project End Date
Aug 31, 2010
Grant Year
2008
Program Code
[8.5]- Food Science & Nutrition
Recipient Organization
IQF DREAM, LLC
(N/A)
HILO,HI 96720
Performing Department
(N/A)
Non Technical Summary
Papaya is a major fruit commodity in Hawaii with over 28 million pounds utilized in 2006. 26.6 million pounds of the harvested papaya are used for fresh utilization and 2.3 million pounds for processed utilization. Processed utilization is currently for aseptic puree produced by a single processor. Additional processing of papaya would be valuable because there are an estimated 3.5 million pounds of culls that can be utilized annually. If not processed into puree, there is no income to the farmer and the packinghouse must dispose of the culls by discarding them, giving them away for livestock feed, or selling them in bulk to a broker for local utilization. The purpose of this project is to utilize these culled papayas and produce a consistently high quality, nutritional Individual Quick Frozen (IQF) product with increased food safety and sufficient stability to retain its value during frozen storage. Utilization of papaya culls would greatly benefit the Hawaii papaya industry and improve the economy of the state's rural communities. Successful project completion will result in a sound, proven concept for processing culled papayas into an IFQ product. This will ensure the microbial security of the product, eliminate the need for quarantine treatment, and preserve fruit quality. An IQF product would provide the industry another avenue to the export market rather than having the culls discarded or sold in the local market. This would allow the industry to establish a niche and compete in the $300 million frozen fruit market.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
50110301103100%
Knowledge Area
501 - New and Improved Food Processing Technologies;

Subject Of Investigation
1030 - Papaya;

Field Of Science
1103 - Other microbiology;
Goals / Objectives
Our goal is to utilize culled papayas grown in Hawaii and produce a consistently high quality Individual Quick Frozen (IQF) product that has excellent sensory qualities and sufficiently stable to retain its value during frozen storage. The objectives of this project is to evaluate the effectiveness that ozone treatment, in conjunction with heat treatment, as microbial reducing methods and the effectiveness of flash freezing in producing a high quality frozen product. The project is designed to answer the following questions: (1) What is the optimal ozone ppm level and time of immersion for microbial reduction; (2) Can the Enterobacter cloacae count be reduced sufficiently; (3) Can the Sodexho standard pathogenic, ACP and coliform counts be achieved by combining ozone treatment with heat treatment microbial reducing methods; (4) Can flash freezing eliminate the potential problem of bounded water and produce a product with no drip; and (5) Can freezing further reduce the microbial counts and maintain fruit sensory quality and for what length of time The overall technical objective of this Phase II application is focused on the technical issues observed during Phase I and on the market research needed for Phase III commercialization. The technical issues observed during Phase I are (1) evaluating the vapor heat process using higher relative humidity (RH) during the heating process to increase the rate of heat transfer, (2) conducting larger volume testing to optimize the time/temperature of the freezing requirements, (3) evaluating the softening of the fruit tissue that may occur during delays between cubing and heating and heating and freezing, and (4) evaluating the storage temperature and the shelf-life of the frozen product. The objectives of Phase II are to answer the following questions: Can the microbial reduction be increased and/or the time of treatment be reduced by using higher relative humidity (RH) currently used in commercial vapor heat quarantine treatment equipment during the heating process and maintain fruit quality; What is the effect of improving the freezing process and using equipment with better heat transfer capabilities; Can cold-storage further reduce the microbial counts and maintain fruit quality and for what length of time; What is the layout and operational requirements for commercial manufacturing; and What is the marketability and economic feasibility The expected output of this project is a layout of a commercial process that not only determines the throughput within the vapor heat and freezing processes but the throughput requirements between each stage of processing including cold storage so that economic feasibility can be assessed. The successful accomplishment of Phase II will allow IQF Dream, LLC to establish a manufacturing process, incorporating technologies new to the Big Island papaya industry, that not only addresses food safety, quarantine regulations and shelf life, but that also enhances the sensory quality of the fruit.
Project Methods
The basic approach to develop an IQF papaya product follows a ten-step process:(1) Choose Kapoho and Rainbow cultivars suitable for processing at the packinghouse. Discard fruits that show visual signs of damage to reduce the incidence of postharvest diseases. (2) Transport chosen papayas to ripening storage area. Select from storage area three quarter to full ripe papayas for processing to capture the best qualities of the fruit.(3) Prior to entry into preparation room, wash the whole fruit using 100-ppm hypochlorite solution and rinse as a GMP sanitization measure. (4) In the certified preparation room, manually slice papayas in half lengthwise. Discard any hard flesh papayas and those showing internal yellowing, purple stain and any other visual diseases/bruising and cleanse utensils. Discarding these papayas will eliminate high initial loads of Enterobacter cloacae and other pathogens. (5) Deseed, slice papaya lengthwise into eights, cube the flesh into the desired size, and peel. By careful removal of the seeds from the cavity of papaya much of the sulfuric off-odors of benzylisothiocyanate can be prevented. Peeling and cubing the fruit produces more surface area for ozone treatment and greatly diminishes disparity and the lag phase in heat transfer resulting in shortened heat treatment and freezing times. (6) Treat the cubed fruit with an ozone wash as a GMP sanitization measure and to reduce the initial microbial count for heat treatment. Using the principles of thermobacteriology of first order death kinetics, a reduced initial microbial load through the selection of sound papayas and the use of the ozone treatment results in reduced subsequent heat temperature and/or treatment times. (7) Treat the cubed fruit with vapor heat. With steps (4) to (6) as preliminary procedures, possible adverse effects on texture and flavor due to this approach is not expected as the proposed heat treatments are within the realm of the quarantine heat treatments which are currently being used commercially for fresh papaya without adverse effects. (8) Treat the cubed fruit with a chilled ozone wash as a GMP sanitization measure and to reduce the temperature of the fruit prior to flash freezing resulting in a shortened time to reach an internal temperature of -28o C. (9) Flash freeze the individual papaya cubes to -28o C and vacuum seal the product. (10) Store packaged frozen products in boxes at a cold-store temperature of -18o C, -20o C or -23o C. While accomplishing various tests using this procedure, we will evaluate the effects of increased humidity in the vapor heat treatment, evaluate the effects of improving the freezing process by controlling the initial temperature of the papaya cubes, evaluate commercial scale volume processing, and evaluate cold storage performance. We will also evaluate the economic feasibility of the product production.

Progress 09/01/08 to 08/31/09

Outputs
OUTPUTS: Tasks are designed to meet our technical objectives by answering the questions: 1.Can the microbial reduction be increased and/or time of treatment reduced by using higher relative humidity (RH) during the heat process and maintain fruit quality; 2.What is the effect of improving the freezing process and using equipment with better heat transfer capabilities; 3.Can cold storage further reduce microbial counts and maintain fruit quality and for what length of time; 4.What is the layout and operational requirements for commercial manufacturing; and 5.What is the marketability and economic feasibility We addressed question 1. Our small-scale vapor heat chamber was modified to ensure typical chamber temperatures of 125F to 130F and RH of 90 to 92%. The efficiency of increased RH from Phase I lie in the difference in the thermal profile during the ramp up time and the smoothing of the thermal fluctuations. Coliform levels were reduced by 2.5D to 3.8D and total aerobic bacteria were reduced by 2.4D to 3.9D. We did sensory observation to evaluate treated fresh (not innoculated) samples for texture, aroma, taste and overall quality. Results did not favor one sample over the other. We have started to address question 2. In a change request, the scope of acquiring a leased spiral blast freezer was changed to leasing the blast freezer used during Phase I. The blast freezer is being leased. The objective remains the same to eliminate softening of the papaya flesh tissue by reducing the initial temperature of the papaya cubes and evaluate different trays and rack placement density affect on the heat transfer capability to freeze the papaya cubes. We have done initial evaluation of solid stainless steel trays, thinner aluminum perforated trays, and perforated trays with non-stick liners to determine which is best to attain an internal papaya cube temperature of -4F and speed up removal of the frozen product from the trays. We have started to answer question 4. We have started to assemble a prototype processing operation. As much as possible, this prototypes an in-line continuous processing and handles volume testing. Designs for conveyor systems for papaya preparation and ozone wash plus the ozone flume are completed and parts ordered. Evaluation of the deseeding operation was started. Vacuuming the seeds with the papaya half in one hand and vacuum hose in the other hand proved successful in 9 out of 10 papayas tested. The one unsuccessful attempt occurred with a papaya having a highly ridged seed cavity. We started design of a fixed housing for the vacuum hose since holding it is not practical for processing operations. Initial consultation was done with State of Hawaii Department of Health and Hawaii County Public Works to define specific requirements for our proposed plant. In October 2008, the commercial plan of this SBIR project was presented at the Hawaii Papaya Industry Annual Conference. In August 2009, an impromptu tour of the prototype processing plant was given to U.S. Congressman Neil Abercrombie and Hawaii Representative Clift Tsuji, Chair of House Committee on Agriculture, with very positive feedback and support. PARTICIPANTS: As part of a technology transfer agreement, the USDA Agriculture Service Pacific Basin Agriculture Center (USDA-ARS PBARC) staff cultivated the Enterobacter cloacae cultures two days prior to inoculation and we inoculated the fresh Rainbow papayas at PARC. Rodney Yonemura served as principal investigator/project director (PI/PD)for this project. He was the lead investigator for all the tasks, did the innoculation at USDA-ARS PBARC, did all the interfacing with State of Hawaii Department of Health and Hawaii County Department of Public Works, oversaw subcontractor and vendor work, and managed the project finances. Dr. Harvey Chan, Jr. assisted in the equipment specifications and the vapor heat treatment. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: The tremendous increases in the price of stainless steel products have caused us to reallocate our budget to handle the increase. This has caused a change in the scope of Task 3 with no change to the objective of the task. The change to Task 3 is the use of an existing blast freezer rather than a new spiral blast freezer.Due to the tough economic times, we had to change technician and out source some stainless steel machinist work.

Impacts
As part of a technology transfer agreement, the USDA Agriculture Service Pacific Basin Agriculture Center (USDA-ARS PBARC) staff cultivated the Enterobacter cloacae cultures two days prior to inoculation and we inoculated the fresh Rainbow papayas at PARC. The papayas were washed in tap water, air-dried, and wiped with 70 percent ethanol to disinfect the surface. The fruits were inoculated at three (3) random sites within a third of the papaya longitudinal transect. Each site was injected with about 0.5 ml of the Enterobacter cloacae culture using a sterile 3 cc. tuberculin syringe and then covered with autoclavable tape. The inoculated papaya fruits were at the third to half ripe stages and allowed to incubate for 2 to 3 days further at IQF Dream, LLC before being subjected to sanitation treatments. Typical infection levels that were attained for both coliform and total aerobic bacteria were at 106 cfu/gm. IQF Dream, LLC was initially delayed due to the uncertainty of the economic downturn, the increase cost of stainless steel items, and the added surcharges on freight costs in 2008. This has caused a slippage in the 2008 and 2009 timelines by three months, but we have made tremendous strides since February 2009 in accomplishing our proposed tasks and meeting our objectives. We therefore plan to be back on track with our proposed schedule by the beginning of 2010. The tremendous increases in the price of stainless steel products have caused us to reallocate our budget to handle the increase. This has caused a change in the scope of Task 3 with no change to the objective of the task. The change to Task 3 is the use of an existing blast freezer rather than a new spiral blast freezer. Due to the tough economic times, our technician had to quit a startup company like IQF Dream, LLC and seek a more stable employment. We therefore have to change technician and out source some stainless steel machinist work.

Publications

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