Source: MICHIGAN TECHNOLOGICAL UNIV submitted to NRP
FUNGAL STIMULATION IN WOOD FROM SUB-LETHAL FUNGICIDE DOSES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0215269
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2008
Project End Date
Sep 30, 2011
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
MICHIGAN TECHNOLOGICAL UNIV
1400 Townsend Drive
HOUGHTON,MI 49931
Performing Department
School of Forest Resources and Environmental Science
Non Technical Summary
Control of basidiomycete decay fungi in treated wood continues to be problematic. Fungicide treatment gradients can leave areas of sub-lethal loadings, which occasionally stimulate fungal growth and/or pigmentation or discoloration of the wood. There has been no attempt thus far to determine at exactly what retentions fungicides, such as CuSO4, tebuconazole, borates, and DDAC, are stimulated. This research will look at fungal stimulation from both the negative view of wood decay for wood in service, and the positive aspect of spalting for artists and woodworkers. The ranges of CuSO4 retentions in white pine and sugar maple required to stimulate growth and pigmentation in Postia placenta, Trametes versicolor, Xylaria polymorpha, Ceratocystis coerulescens, and Arthrographis cuboidea will be determined. Decay testing will also be performed using combinations of two fungi on treated wood to determine if copper can control basidiomycete growth while allowing or even stimulating pigmentation of staining fungi. Surface applications of CuSO4 will be applied to sugar maple and white pine boards to test topical control of fungi for artistic purposes. Similar tests will also be performed using tebuconazole, borates, and DDAC. Overall, this research aims to use wood preservatives as stimulants and controlling agents for spalting, while also identifying specific retentions that stimulate decay so that these ranges can be minimized in industrial and outdoor wood applications.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
51153201103100%
Goals / Objectives
Overall Objective: Determine the retention of various fungicides, particularly Cu++, which stimulate fungal growth. Achieving this objective will be used to reduce incidental fungal stimulation in commercial treated wood caused by preservative loading gradients, and also to control and stimulate spalting fungi. Specific Objectives: 1. Determine the retention(s) of CuSO4 which stimulate growth and pigmentation of Trametesversicolor, Postia placenta, Xylaria polymorpha, Ceratocystis coerulescens, and Arthrographis cuboidea in eastern white pine and sugar maple. 2. Evaluate methods to minimize stimulating CuSO4 retentions within a copper gradient in wood treated with commercial waterborne preservatives. 3. Determine CuSO4 retentions, which are high enough to minimize basidiomycete decay, while still allowing or even stimulating growth of spalting/sapstain fungi. 4. Develop techniques to bring spalting into the emerging field of bio-art by using topical applications of CuSO4 to 'draw' areas of exclusion and stimulation on wood. 5. Evaluate other fungicides, particularly tebuconazole, boric acid, and DDAC, for fungal growth stimulation at low loadings.
Project Methods
There will be four stages to the research: 1)Preliminary decay testing of 14 mm cube blocks using widely spaced retentions. 2)Decay testing of 14 mm cube blocks within a narrow range of retentions. 3) Multiple fungi decay testing of 37 mm3 blocks. 4)Decay testing 1 x 4 x 6 boards with small surface treatments of fungicide. More detail is provided in the full proposal.

Progress 07/01/08 to 09/30/11

Outputs
OUTPUTS: Spalting is the aesthetic enhancement of wood using fungal growth. The objective of this project was to use low doses of fungicides to control the spalting process. Studies were performed on the effects sub-lethal doses of copper (II), boric acid, tebuconazole, and a quaternary ammonium salt on fungal growth in a solid wood medium. Statistical analysis were conducted and results written into manuscripts. Dual fungal inoculations and their interactions with low level copper sulfate were also determined. Several large-scale surface treatment tests were carried out with copper sulfate and Trametes versicolor on sugar maple. The data were disseminated via publications. PARTICIPANTS: Sara Robinson and undergraduate research assistants TARGET AUDIENCES: The wood mycology scientific community. The amateur woodworking community. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
The use of low-level copper sulfate in sugar maple blocks inoculated with two fungi changed the performance of both fungi. In Scytalidium cuboideum/Xylaria polymorpha interactions, copper sulfate caused S. cuboideum to produce more melanin, and also produce pink zone lines. In Ceratocystis virescens/Xylaria polymorpha, copper sulfate restricted blue stain of C. virescens to only areas already degraded by X. polymorpha. In addition, low levels of copper sulfate pressure treated into rough-turned bowls of sugar maple allowed for sectional surface degradation by Trametes versicolor, resulting in a texturized surface similar to sand blasting. Other fungicides and fungal species tested had less impact on wood appearance.

Publications

  • Robinson, S.C. 2011. Destroying uniformity: Using fungi to add a tactile and visual experience to functional wood. Leonardo Journal 44(2):145-151.
  • Robinson, S.C., Laks, P.E. 2011. The effects of copper in large scale mono- and dual-fungus wood systems. Forest Products Journal 60(6):490-495.
  • Robinson, S.C., Laks, P.E., Richter, D.L. 2011. Stimulating spalting in sugar maple using sub-lethal doses of copper. European Journal of Wood and Wood Products 69(4):527-532.


Progress 10/01/10 to 09/30/11

Outputs
OUTPUTS: Statistical analysis was conducted on previously collected data, and results written into manuscripts. Dual inoculations and their interactions with low level copper sulfate was also determined. Several large-scale surface treatment tests were carried out with copper sulfate and Trametes versicolor on sugar maple. The data were disseminated via publications. PARTICIPANTS: 1. Sara Robinson 2. Undergraduate research assistants TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The use of low-level copper sulfate on sugar maple blocks with multiple fungi changed the performance of both fungi. In Scytalidium cuboideum/Xylaria polymorpha interactions, copper sulfate caused S. cuboideum to produce more melanin, and also produce pink zone lines. In Ceratocystis virescens/Xylaria polymorpha, copper sulfate restricted blue stain of C. virescens to only areas already degraded by X. polymorpha. In addition, low levels of copper sulfate pressure treated into rough-turned bowls of sugar maple allowed for sectional surface degradation by Trametes versicolor, resulting in a texturized surface similar to sand blasting.

Publications

  • Robinson, S.C. 2011. Destroying uniformity: Using fungi to add a tactile and visual experience to functional wood. Leonardo Journal 44(2):145-151.
  • Robinson, S.C., Laks, P.E. 2011. The effects of copper in large scale mono- and dual-fungus wood systems. Forest Products Journal 60(6):490-495.
  • Robinson, S.C., Laks, P.E., Richter, D.L. 2011. Stimulating spalting in sugar maple using sub-lethal doses of copper. European Journal of Wood and Wood Products 69(4):527-532.


Progress 10/01/09 to 09/30/10

Outputs
OUTPUTS: Data were collected on weight loss and spalting produced by Trametes versicolor, Xylaria polymorpha, Postia placenta, Arthrographis cuboidea, and Ceratocystis virescens on 14 mm cubes of sugar maple and white pine treated with tebuconazole, DDAC, or boric acid. Experiments were conducted on exclusionary spalting with the use of copper sulfate as a topical treatment. The data were disseminated via publications, and one graduate student was supported for one year. PARTICIPANTS: Undergraduate research assistants were utilized for color analysis, block preparation, and fungal culturing. Students learned how to use the Scion Image protocol, how to culture and care for fungal cultures, and how to pressure treat lumber. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
More weight loss was found with white pine cubes inoculated with Postia placenta and treated with sub-lethal levels of tebuconazole than on control cubes. DDAC and boric acid retentions did not stimulate weight loss. The retention of 1.0 kg/m3 copper sulfate, when applied topically, successfully excluded growth of Xylaria polymorpha in a small area, allowing for designs to be placed on sugar maple lumber.

Publications

  • Robinson, S.C., Laks, P.E. 2010. The Effects of Sub-threshold Loadings of Tebuconazole, DDAC and Boric Acid on Wood Decay by Postia placenta. Holzforschung 64(4):537-543.
  • Robinson, S.C., Laks, P.E. 2010. Practical Spalting of Wood Using Copper. Coloration Technology, in press.


Progress 10/01/08 to 09/30/09

Outputs
OUTPUTS: Data were collected on weight loss and spalting produced by Trametes versicolor, Xylaria polymorpha, Postia placenta, Arthrographis cuboidea, and Ceratocystis virescens on 14 mm cubes of sugar maple and white pine treated with copper sulfate. Data were also collected on spalting produced by dual inoculation of aforementioned fungi on 37 mm sugar maple cube blocks treat with copper sulfate. The data were disseminated via publications. PARTICIPANTS: Undergraduate research assistants were utilized for color analysis, block preparation, and fungal culturing. Students learned how to use the Scion Image protocol, how to culture and care for fungal cultures, and how to pressure treat lumber. TARGET AUDIENCES: Our target audience is the general public and the wood protection industry. The latter includes chemical manufacturers, product formulators, treating companies, and forest products companies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
A protocol was developed that utilized Scion Image as a color analysis tool for evaluating the amount of spalting on each cube. This protocol allowed for a less subjective method of determining the amount of spalting on each piece of wood. The laboratory resources available allowed for a large scale evaluation of the effectiveness of soil and vermiculite as incubation mediums. The mediums were then used based upon their attributes - when decay was desired, soil was used, and when spalting was desired, vermiculite was used. Copper sulfate stimulated zone line production on sugar maple with Xylaria polymorpha, and caused Arthrographis cuboidea to produce orange instead of pink pigment. Dual inoculations in the presence of copper produced pink zone lines (Arthrographis cuboidea / Xylaria polymorpha).

Publications

  • Robinson, S.C., Laks, P.E. and Richter, D.L. 2009. Stimulating spalting in sugar maple using sub-lethal doses of copper. European Journal of Wood and Wood Products in review.
  • Robinson, S.C., Laks, P.E., Turnquist, E.J. 2009. A method for digital color analysis of spalted wood using Scion Image software. Materials 2(1): 62-75.
  • Robinson, S.C., Richter, D.L., Laks, P.E. 2009. Effects of substrate on laboratory spalting of sugar maple. Holzforschung 63: 491-495.