Source: UNIV OF IDAHO submitted to NRP
MARKER-ASSISTED BREEDING OF FUSARIUM HEAD BLIGHT RESISTANCE IN WHEAT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0214574
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2008
Project End Date
Jun 30, 2013
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF IDAHO
875 PERIMETER DRIVE
MOSCOW,ID 83844-9803
Performing Department
Plant Soil & Entomological Sci
Non Technical Summary
Fusarium head blight (FHB) is one of the most destructive diseases of wheat (Triticum aestivum L. and T. durum L.) and barley (Hordeum vulgare L.) in warm and humid areas of the world (Schroeder and Christensen, 1963; Wang et al., 1982; Snijders 1990). FHB epidemics can cause significant yield losses, shriveled kernels, and deposition of vomitoxin (Deoxynivalenol (DON)) in the infected seeds which renders the grain unsuitable for human consumption and feed (McMullen et al., 1997). Wheat and barley losses caused by FHB epidemics in the USA during the 1990s were estimated at close to $3 billion U.S. dollars (Windels, 2000). The development and deployment of resistant germplasm is an environmentally sustainable method for the control of FHB. Several species of the Fusarium genus are known to cause FHB (Wiese, 1987). F. graminearum and F. culmorum are the primary Fusarium species causing DON contamination and reduced grain quality. F. graminearum survives between wheat crops in living or dead host tissues (Zhu and Fan, 1989). Wheat planted after maize often has significantly more head blight than wheat planted after other crops. Therefore, reduced tillage in soil conservation systems increases the amount of inoculum present (Teich and Hamilton, 1985). Wheat production in Idaho ranks 5th in the nation in 2006. Adding FHB resistance to current cultivars is critical and valuable for our growers to maintain sustainable high quality grain production in national and world markets. FHB epidemics occurred in sprinkler-irrigated wheat and barley fields in south central and eastern Idaho in 1982 and 1984, resulting in estimated yield losses as high as 50% (Michuta-Grimm and Foster, 1989). Scab epidemics occurred in sprinkler-irrigated spring wheat and barley fields in Montana in 2006 and 07 and resulted in estimated yield losses over 50% (Luther Talbert, personal communication). With the increase in corn production (for dairy and biofuel), and no-till or conservation tillage in the area, it would be beneficial to have an active FHB resistance program to insure that future cultivars carry some resistance if corn production, especially under no-till, continues to increase in acreage in the south central and south eastern part of the state. Changing production practices and increasing temperatures in the PNW, as predicted by climate models, may increase the risk of FHB. Therefore, incorporation of FHB resistance into existing germplasm may prevent or reduce future losses.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2011549108040%
2021549108030%
2121549108130%
Goals / Objectives
The ultimate objective of this proposal is to develop desirable wheat cultivars with value-added FHB resistance via marker assisted selection (MAS). The specific objectives include: 1) Evaluate and characterize FHB and DON resistance in PNW cultivars and advanced lines, identify the presence of known FHB QTL and infer potentially novel source of resistance through allele size comparison of marker loci identified in known resistance sources; 2)Evaluate and characterize type II and DON resistance of 2,400 headrows in five segregating populations. FHB resistance of 238 PNW spring and winter wheat cultivars and lines will be compared with 8 reference cultivars and lines which have been previously characterized for FHB resistance. A total of 15 markers on six chromosome regions (2D, 3A, 3BS, 5AS, and 6B) will be used in characterization of FHB resistance in 238 genotypes via haplotyping. Additional 15 markers will also be applied in the 238 genotypes for haplotyping of other agronomic traits, including protein content, baking quality, and PNW prevalent diseases. Approximately 7140 data points will be generated using ABI gene analyzer and conducted in collaboration with Deven See in genotyping center at Pullman, Washington. The 238 cultivars and lines will be evaluated for FHB resistance via floret inoculation method in an irrigated nursery in Aberdeen. FHB resistance of 50 spring wheat lines will also be evaluated by Dr. Luther Talbert in Montana and by Dr. Chur Hollingsworth in Minnesota. Seed samples of 238 entries will be sent for DON analysis to Dr. Yanhong Dong at University of Minnesota. In addition, all entries in this experiment will be evaluated in a minimum of two dryland and two irrigated locations in Idaho and/or in PNW region for agronomic performance including yield, disease resistance to prevalent pathogens other than FHB, and milling and baking quality after harvesting. MAS of the 15 markers used in above experiment will also be applied in 2400 headrows in five populations, producing 36,000 data points. Selected headrows having desirable QTL combinations and good agronomic performance will be evaluated for FHB resistance in replicated tests in subsequent year. The selected headrows will also be shared with other breeders in PNW region. This project has potential to identify novel and adapted resistance sources, and improve the effiency of selection of FHB resistance. The identified cultivars/lines having good field FHB resistance and/or known FHB resistance QTL can then be grown in PNW region and used as adapted resistance sources to PNW and Great Plains breeding programs.
Project Methods
FHB resistance of 238 PNW spring and winter wheat cultivars and lines will be compared with 8 reference cultivars/lines which have been previously characterized for FHB resistance. A total of 15 markers on six chromosome regions (2D, 3A, 3BS, 5AS, and 6B) will be used in characterization of FHB resistance in 238 genotypes via haplotyping. Additionally 15 markers will also be applied in the 238 genotypes for haplotyping of other agronomic traits, including protein content, baking quality, and PNW-prevalent diseases. Approximately 7,140 data points will be generated and conducted in collaboration with Deven See at the genotyping center at Washington State University, Pullman, Washington. The 238 cultivars/lines will be evaluated for FHB resistance using the single floret inoculation method in an irrigated nursery in Aberdeen. FHB resistance of 50 spring wheat lines will also be evaluated by Dr. Luther Talbert in Montana and by Dr. Chur Hollingsworth in Minnesota. Seed samples of 238 entries will be sent for DON analysis to Dr. Yanhong Dong at the University of Minnesota. In addition, all entries in this experiment will be evaluated in a minimum of two dryland and two irrigated locations in Idaho and/or in the PNW region for agronomic performance including yield, disease resistance to prevalent pathogens other than FHB, and milling and baking quality after harvesting. The original inoculum will be isolated from infected wheat grains harvested from Twin Falls, Aberdeen, and Hazelton where scab infection was observed this year in Idaho. Then the mixed cultures will be used for conidial production. The nursery at Aberdeen will receive overhead fine mist irrigation after inoculation for approximately one month. Scab incidence, severity, index, and DON concentration will be assessed. Plant DNA will be extracted using a high throughput equipment Geno Grinder 2000-115. DNA marker polymorphism will be detected with a cost efficient high throughput gel electrophoresis system conducting a minimal of four hundred DNA samples a day without using ABI system. MAS of five populations will be conducted in our lab located in the USDA building in Aberdeen. Selected headrows having desirable QTL combinations and good agronomic performance will be evaluated for FHB resistance in replicated tests in subsequent years. Additional F1 plants will be made in the spring of 2008 on the basis of haplotype complementation upon the completion of haplotying in PNW wheat. Data from replicated trials will be analyzed using a standard ANOVA, and significance of means will be established based on LSD or other appropriate tests. Associations between factors will be determined based on correlation analyses. All data analysis will be performed using SAS Software. Newly identified PNW wheat genotypes with FHB resistance will be released as cultivars or germplasm and provided to breeders for use as parents in their programs. Where appropriate, data will be presented and/or published in the Proceedings of the National FHB Forum or in refereed journals. All facilities and equipment necessary for conducting the proposed project are available.

Progress 07/01/08 to 06/30/13

Outputs
Target Audience: Cereal Grain Producers, Breeders, Pathologists, Students and Scientists who are working on head blight. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Through this project one technician, one post doctorate, and one graduate student learned how to isolate pathogen and produce inoculum and screen FHB resistance in greenhouse and field nurseries. They also received training how to analyze field and genomic marker data. The graduate student received extensive training on high throughput genotyping in a regional genotyping lab. In addition, they presented posters at several professional meetings. The graduate student being worked on this project has been supported by NIFA National Need Fellowship and will graduate in the spring of 2014. How have the results been disseminated to communities of interest? We presented the results at several professional meetings and published in Idaho Grain Magazine and Crop Scicene as well as at growers field days. What do you plan to do during the next reporting period to accomplish the goals? As we see more FHB infection in this region it is essential to set up field nurseries in the breeding program so that we can assess more elite lines and many segregating populations. We are also going to continue using molecular marker assisted selection to accelerate phenotypic selection using markers identified in UI Stone and from previously published references. At present we have made over 200 populations derived from crosses between Sumai 3, W14, and resistance sources from National Germplasm Collection (NSGC) in adapted backgrounds. We are going to assess FHB resistance and select resistant progeny and cultivars in these populations in field nurseries from 2014 to 2018.

Impacts
What was accomplished under these goals? Fusarium head blight (FHB) is an emerging fungal disease of wheat and barley in Idaho and Pacific Northwest (PNW). There is limited information on resistance or susceptibility in cultivars grown in this region. There is a risk to spread disease if we begin setting up disease nursery in field. Therefore, we initially conducted an assessment of FHB resistance using molecular markers associated with known FHB-QTL (2B, 2D, 3A, 3BS, 5AS, and 6B) for 238 spring and winter wheat lines historically grown in Idaho and PNW. Compared to the eight resistant cultivars we found some winter and spring wheat lines had resistant alleles same as know resistance sources Sumai 3 orW14 or Ernie. To confirm the function phenotype of these lines we established collaborative disease nurseries in multiple states and greenhouse nursery in our facility. The winter wheat lines were assessed for FHB resistance in a disease nursery at Virginia Tech under collaboration with Drs. Griffey and Liu in 2009. The 50 spring wheat lines were assessed for FHB resistance in disease nurseries in Montana State University, University of Minnesota, and in our greenhouse nurseries from 2009 to 2012. The results from these screenings were presented in several professional meetings and published in Idaho Grain Magazine. Based on marker data and disease data we identified a resistant line IDO599 and released as UI Stone in 2012. This cultivar was filed a PVP application in 2013 and has been marketed by LimaGrain Cereal Seeds. A mapping population between UI Stone and Alturas was developed and assessed for FHB resistance in three experiments in greenhouse by the spring of 2013. This population is being genotyed by a graduate student using SSR, SNP, and GBS marker systems. In 2012, we assessed 100 hard white accessions from National Small Collection in conjunction with the Triticeae CAP and found 13 accessions with good level of resistance. These lines were then used in crossing in the breeding program. At present, we have made over 200 populations derived from crosses between Sumai3, W14, and resistance sources from NSGC in adapted backgrounds.

Publications

  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Liu, Sh., C.A. Griffey, M.D. Hall, A. L. McKendry, J. Chen, W.S. Brooks, G. Brown?Guedira, D. Van Sanford, D.G. Schmale. 2013. Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars. Theor Appl Genet DOI 10.1007/s00122-013-2149-y
  • Type: Journal Articles Status: Published Year Published: 2012 Citation: Chen, J., J. Wheeler, J. Clayton, W. Zhao, K. OBrien, C. Jackson, J. M. Marshall, B.D. Brown, K. Campbell, X.M. Chen, R. Zemetra, and E.J. Souza. 2013. Registration of UI Stone Wheat. J. Plant Registration 7: 321-326.


Progress 01/01/12 to 12/31/12

Outputs
OUTPUTS: FHB is one of the most destructive diseases of wheat and causes significant reductions in grain yield and quality in the world. It is an emerging disease in southern Idaho and some areas in PNW due to the increasing corn production and global climate changes. We firstly applied MAS and genotyped 376 cultivars or breeding lines from Idaho and PNW wheat breeding programs with five markers on chromosomes 3B (Fhb1) and 5A (Fhb3) associated with FHB resistance in collaboration with genotyping center at USDA ARS, Washington State University. We presented this genotyping work at FHB forum in 2008 In order to confirm the resistance evaluated by molecular markers we conducted phenotypic evaluation for 50 spring wheat lines through collaborations with University of Minnesota in 2008 and Montana State University in 2009 and 2010. In 2010, we established a greenhouse screening system and in 2011, we established a field screening system in Aberdeen, ID. Using GH screening system, we confirmed FHB resistance in three GH experiments for the 50 spring wheat cultivars and advanced lines that previously evaluated in nurseries at University of Minnesota and Montana State University. We presented this evaluation at Idaho Cereal Schools in 2012. UI Stone (IDO599) soft white spring wheat cultivar was identified having known resistant marker alleles at Fhb1 and Fhb3 loci and has high level of type II resistance to FHB. One mapping population was developed from UI Stone x Alturas to identify novel QTL other than Fhb1 and Fhb3 in UI Stone. Two FHB screening of this population were conducted in GH in 2012. This population will be planted in a replicated yield trial in field in 2013. Another hard red spring wheat cultivar Lassik had resistant marker allele at Fhb1 locus and moderate resistance to FHB. Lassik also has very good resistance to stripe rust (Yr36) and high protein gene GpcB1. Therefore, Lassik was crossed with lines with resistance to Hessian Fly (H25) to pyramid Fhb1, Yr36, GpcB1, and H25. The F2 and F3 progeny of these crosses were assessed via MAS and FHB screening in GH in 2011. The F4 progeny was evaluated for FHB resistance and agronomic performance in 2012. Advanced lines were developed which have target resistant alleles and good yield performance. PARTICIPANTS: J. Chen, PI, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Oversee the project performance and supervise students and technician to conduct field and marker evaluation. J. Marshall, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Collaborate with Dr. Chen and identify F. Graminearum and F. Culmorum in the field. J. Wheeler, support scientist, and Y. Wang, post-doctorate, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Mr. Wheeler and Dr. Wang assisted greenhouse and field screening and DNA extraction. D. See, Western Regional Small Grain Genotyping Laboratory, USDA-ARS, Pullman, WA. Conduct genotyping of markers associated with FHB resistance in cooperation with Dr. Jianli Chen. Mr. Santosh Nayak is a MS student being trained by this project and his assistantship is supported by USDA NIFA National Need Fellowship. He is responsible for mapping of FHB resistance in UI Stone. This project also provided training to one Post Doctorate and one college student in 2011 and 2012. TARGET AUDIENCES: Cereal Grain Producers, breeders, pathologists, students PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Acknowledging to multi-state collaboration and molecular marker-assisted selection, UI Stone, the newly released soft white spring wheat cultivar was identified having high level of type II resistance to Fusarium head blight. This cultivar also has high grain yield and consistent end-use quality under rain-fed and irrigated conditions, good resistance and/or tolerance to stripe rust and cereal cyst nematodes. The release of this cultivar would help growers in Idaho and Pacific Northwest to fight FHB when it occurs. By using MAS and newly established greenhouse and field screening systems, we are able to develop some advanced lines pyramided FHB resistance with resistances to stripe rust, Hessian Fly, and end-use quality in three years. Adapted resistant lines, screening systems, and molecular markers would accelerate releasing of more resistant cultivars in the future. This will eventually improve economic conditions for growers to reduce disease losses, and reduce environmental impacts with less foliar fungicide treatments needed to control disease. In addition, one graduate student has been trained through this project.

Publications

  • Chen, J., Wheeler, J., Clayton, J, Zhao, W., O Brien, K., Jackson, C., Marshall, J.M., Brown, B.D., Campbell, K., Chen, X.M., Zemetra, R. and Souza, E.J. 2012. Registration of UI Stone Wheat. J. Plant Registration (In Review).
  • Liu, S.Y., Griffey, C.A., Hall, M.D., Chen, J., Liu, S., Tucker, D. and Brooks, W.S. 2012. Registration of Becker/Massey Wheat Recombinant Inbred Line Mapping Population. J. Plant Registration 3:358-362.
  • Chen, J., Wheeler, J., Zhao, W. and Marshall, J.M. 2011. Evaluation of resistance to Fusarium head blight in fifty spring wheat cultivars and advanced lines grown in Pacific Northwest. 2011 Small Grains Report http://www.extension.uidaho.edu/scseidaho/.
  • Marshall, J., Bissonnette, K. and Chen, J. 2012. The re-emergence of Fusarium Head Blight in Idaho, 30-12 UI Extension Publication.


Progress 01/01/11 to 12/31/11

Outputs
OUTPUTS: FHB is one of the most destructive diseases of wheat and causes significant reductions in grain yield and quality in the world. However, FHB is a new wheat disease in Idaho and evaluation of FHB resistance can be difficult using phenotypic evaluation as the disease nursery can't be set up in the field. This project at Aberdeen applied marker-assisted evaluation as an alternative of phenotypic evaluation. In 2011, our focus was to evaluate FHB resistance in F2 progeny derived from a resistant cultivar Lassik identified in previous two years and to validate the greenhouse and field screening protocols established in 2009 and 2010. A total of 752 F2 progeny were evaluated with over 15 molecular markers associated with resistance to FHB, stripe rust, Hessian fly, and markers associated with baking quality in collaboration with genotyping center at USDA-ARS, Washington State University. The progeny were also planted in a field nursery and evaluated for agronomic performance. Some of them (100) were screened for resistance to FHB in a controlled field environment and resistance to Hessian fly in controlled lab environment. In addition, over 150 elite lines were screened for FHB resistance in three greenhouse (GH) experiments. PARTICIPANTS: J. Chen, PI, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Oversee the project performance and supervise students and technician to conduct field and marker evaluation. J. Marshall, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Collaborate with Dr. Chen and identify F. Graminearum in the field. J. Wheeler, support scientist, and W. Zhao, lab technician, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Mr. Wheeler was responsible for greenhouse screening and Mr. Zhao was responsible for DNA extraction and field screening. D. See, Western Regional Small Grain Genotyping Laboratory, USDA-ARS, Pullman, WA. Conduct genotyping of markers associated with FHB resistance in cooperation with Dr. Jianli Chen. This project provided training to one Ph.D and one college student in summer 2011. TARGET AUDIENCES: Cereal Grain Producers, breeders, pathologists PROJECT MODIFICATIONS: As we have established screening protocols for greenhouse and field and identified some of FHB-resistant lines we are going to focus on pyramiding of FHB resistance with resistance to stripe rust, stem rust, Hessian fly, resistance to late maturity alpha amylase, and desirable end-use quality in coming years. Additional new crosses were made in field in summer 2011. These crosses were made for pyramiding of stripe rust, stem rust, FHB, and cyst nematodes. The F1s have been planted in greenhouse and currently being back or top-crossed to elite lines. The F2 and BC1F1 will be planted in spring 2012. We also expect to accelerate the development of hard white spring wheat varieties using the new germplasm from NSGC and CIMMYT.

Impacts
A total of 50 spring wheat cultivars and advanced lines were previously evaluated via MAS and in two field (2008 and 2009) and one GH experiments (2010). In 2011, these materials were evaluated for FHB resistance in two GH experiments. FHB resistance of these materials was characterized based on the mean resistance of the 50 lines. DON content smaller than 2 ppm, severity smaller than 26% in field and 31.5% in GH were suggested as resistant reaction. Out of 50 lines evaluated, ten PNW lines (Whitebird, IDO629, Otis, Lolo, IDO599, IDO686, IDO668, IDO671, Penawawa, and Lassik) and two resistance checks had good resistance in the two field and three GH experiments and DON resistance. IDO599, IDO668, IDO686, and IDO671 are potential new releases of soft white spring wheat. Lassik is a hard red spring wheat cultivar released by University of California, Davis (UCD). Otis is a hard white spring wheat cultivar released by Washington State University (WSU). Lolo and Whitebird are two soft white spring wheat cultivars released by the University of Idaho (UI). Penawawa is a soft white spring wheat released by Washington State University. An additional eight cultivars and lines have good resistance in the two field experiments and low DON content and showed a susceptible reaction in the three GH experiments. In addition, four of the 50 have good resistance in the field and in GH ; while four have good resistance to DON and in the GH . Molecular markers were successfully used and accelerated in pyramiding of FHB resistance with resistance to stripe rust and Hessian fly as well as end-use quality. In 2011, we had severe stripe rust epidemic in field nurseries in Aberdeen. The 752 F2 progeny evaluated showed very good resistance to stripe rust and most of them have short plant height. Over 300 F3 lines were selected and harvested. These lines possess combined markers associated with resistance to FHB, stripe rust, Hessian fly, and end-use quality. In 2012, these lines will be screened for resistance to FHB in greenhouse and for resistance to stripe rust in field nurseries, at the same time be planted for yield and agronomic evaluation. Some of these lines will be selected and harvested on the basis of field performance, yield, bread baking quality, and marker genotypes. Both hard white and red spring wheat lines will be derived from these lines and can be released for irrigation production if desired. In addition to above breeding activities, we initiated a mapping population from a cross IDO599 x Alturas to identify QTL and molecular markers associated FHB resistance in IDO599 and HTAP stripe rust resistance in Alturas in coming years. IDO599 is going to be released as a FHB resistant soft white spring wheat cultivar. Alturas is a widely grown soft white spring wheat cultivar that has HTAP resistance to stripe rust for many years.

Publications

  • Chen, J., Griffey, C.A, Liu, Sh., and Saghai Maroof, M.A. 2012. Registration of VA04W-433 and VA04W-474 FHB Resistant Wheat Germplasm. J. Plant Registration 6 (1): 111-116.
  • Chen, J., Wheeler, J., Zhao, W., Grey, W., Hollingsworth, C.R., See, D., and Marshall, J. 2012. Evaluation of resistance to fusarium head blight in fifty spring wheat cultivars and advanced lines grown in Pacific Northwest. Submitted to Annual Wheat News Letter.
  • Chen, J., Souza, E.J., Hole, D., Guttieri, M.J., O Brien, K., Wheeler, J., Sorensen, L., Clayton, J., Zemetra, R., Windes, J.M., and Chen, X. 2012. Registration of UI SRG Wheat. J. Plant Registration 6 (1): 66-70.
  • Chen, J., Chu, Ch., Souza, E.J., Guttieri, M.J., Chen, X., Xu, S., Hole, D., and Zemetra, R. 2011. Whole genome-wide mapping for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a hard red winter wheat germplasm IDO444. Molecular Breeding (DOI 10.1007/s11032-011-9590-x).
  • Li, P., Chen, J., Wu, P., Zhang, J., Chu, Ch., See, D., Brown-Guedira, G., Zemetra, R., and Souza, E. 2011. QTL analysis for the effect of RhtB1 dwarfing gene on coleoptiles length, seedling root length and numbers of bread wheat (Triticum aestivum L.). Crop Sci 51: 2561-2568.
  • Li. P., Chen, J., and Wu, P. 2011. Agronomic characteristics and grain yield of 30 spring wheat genotypes under drought stress and non-stress conditions. Agronomy Journal 103 (6): 1619-1628.


Progress 01/01/10 to 12/31/10

Outputs
OUTPUTS: FHB is one of the most destructive diseases of wheat and causes significant reductions in grain yield and quality in the world. However, FHB is a new wheat disease and evaluation of FHB resistance can be difficult using phenotypic evaluation as the disease nursery can't be set up in the field in Idaho. This project at Aberdeen applied marker-assisted evaluation (MSE) as an alternative of phenotypic evaluation. In 2010, we evaluated 376 breeding lines with five markers on chromosomes 3B and 5A associated with FHB resistance in collaboration with genotyping center at USDA-ARS, Washington State University. In order to confirm the resistance evaluated by molecular markers we conducted phenotypic evaluation for 50 spring wheat lines through collaborations with University of Minnesota (UM) in 2008 and Montana State University (MSU) in 2009, and for 16 winter wheat lines with Virginia Tech (VT) in 2009 and 2010. In 2010, we also modified our greenhouse, installed two misting benches, and conducted one screening of the 50 spring wheat cultivars and advanced lines that previously evaluated in nurseries at UM and MSU. PARTICIPANTS: J. Chen, PI, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Oversee the project performance and supervise students and technician to conduct field and marker evaluation. D. See, Western Regional Small Grain Genotyping Laboratory, USDA-ARS, Pullman, WA. Conduct genotyping of markers associated with FHB resistance in cooperation with Dr. Jianli Chen. C.R. Hollingsworth, University of Minnesota Northwest Research & Outreach Center, Crookston, MN. Evaluated 50 spring wheat lines for field FHB resistance in cooperation with Dr. Chen in 2008. William Grey, Montana State University, Bozeman, MT. Evaluated 50 spring wheat lines for field FHB resistance in cooperation with Dr. Chen in 2009. Shuyu Liu and Carl A. Griffey, Virginia Tech, Blacksburg, VA. Evaluated 16 winter wheat lines for FHB resistance in cooperation with Dr. Chen in 2009 and 2010. Robert Zemetra and Juliet Windes, University of Idaho collaborators on variety development. TARGET AUDIENCES: Cereal Grain Producers, breeders, pathologists PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Based on the field data derived the multi-state nurseries and marker data from genotyping center, we identified 23 spring wheat cultivars resistant to FHB, of which 8 (35%) spring wheat possess known resistant QTL on 3BS and 5AS. Out of 16 winter wheat cultivar/lines evaluated in VT, seven have 3BS and 5AS QTL, eight lines with 3BS, one with 5AS, and one with none of 3BS and 5AS. The sixteen lines all have good resistance to disease spread with the mean severity data of two years smaller than 25%, and moderately resistance to initial infection with the mean infection data smaller than 40%. However, only four of them have DON content less than 2 PPM. Molecular markers were used in variety releases. IDO599, a soft white spring wheat line was found to have FHB1 gene to FHB and will be released in 2011. Lassik released by UC Davis was also identified having FHB1 gene. Lassik and IDO599 have been used in crosses to pyramid FHB resistance with high protein content, Hessian fly resistance, and resistance to stripe rust. Molecular marker-assisted evaluation has been confirmed as a primary alternative of phenotypic evaluation for the areas where the FHB is a new disease or emerging disease. FHB resistant lines identified from the current study will help growers to fight FHB if it occurs. This will eventually improve economic conditions for growers to reduce disease losses, and reduce environmental impacts with less foliar fungicide treatments needed to control disease.

Publications

  • No publications reported this period


Progress 01/01/09 to 12/31/09

Outputs
OUTPUTS: Scab infection was high in the field plots at Aberdeen in 2009 because of high precipitation occurred in June. One mapping population derived from the cross Coda x Brundage was evaluated with disease severity. The field grown this population was close to a disease nursery for dryland foot rot. Field infection was very common and had lower disease severity for other plots away from this field. A set of pictures of the field infection were taken and disseminated to Idaho Wheat Commissioners at a commission meeting held in Idaho Falls and to growers at field days as well as to researchers working on barley and wheat. We also conducted two field nurseries this year. One nursery was to evaluate for 50 spring wheat in collaboration with Montana State University (MSU, and another was to evaluate 16 winter wheat lines in collaboration with Virginia Tech (VT). These lines were selected based on the results of marker-assisted evaluation of 276 wheat genotypes in 2008. The field nurseries were conducted to confirm if the marker-assisted lines have the function of the genes selected by markers. One manuscript derived from data of the previous two years is in progressing and will be submitted in 2010. Molecular markers were used in variety releases and protection. The new released cultivar 'UI Silver' was identified having FHB1 gene to Fusarium head blight, resistance to disease spread. It showed low disease severity evaluated at VT disease nursery. Training on mapping and marker-assisted selection and FHB field evaluation were provided by the project leader to one UI Ph.D student and one visiting Ph.D student from China as well as two support technician from the breeding program. Dissemination. Knowledge on the identification of FHB infection was disseminated to Idaho Wheat Commissioners at a commission meeting held in Idaho Falls and to growers at field days as well as to researchers working on barley and wheat by pictures and field demonstrations. PARTICIPANTS: J. Chen, PI, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Oversee the project performance and supervise students and technician to conduct field and marker evaluation. D. See, Western Regional Small Grain Genotyping Laboratory, USDA-ARS, Pullman, WA. Conduct genotyping of markers associated with FHB resistance in cooperation with Dr. Jianli Chen. C.R. Hollingsworth, University of Minnesota Northwest Research & Outreach Center, Crookston, MN. Evaluated 50 spring wheat lines for field FHB resistance in cooperation with Dr. Chen in 2008. William Grey, Montana State University, Bozeman, MT. Evaluated 50 spring wheat lines for field FHB resistance in cooperation with Dr. Chen in 2009. Shuyu Liu and Carl A. Griffey, Virginia Tech, Blacksburg, VA. Evaluated 16 winter wheat lines for FHB resistance in cooperation with Dr. Chen in 2009. Robert Zemetra and Juliet Windes, University of Idaho collaborators on variety development. TARGET AUDIENCES: U.S. Wheat workers and growers, especially in the PNW region. PROJECT MODIFICATIONS: Field nursery in Aberdeen proposed in the original proposal has not been set up because of the concerns to spread disease in Idaho. We are planning to do a screening in greenhouse in 2010 when budget becomes available from Idaho Wheat Commission.

Impacts
Molecular marker-assisted evaluation has been confirmed as a primary alternative of phenotypic evaluation for the areas where the FHB is a new disease or emerging disease. Some of the Idaho and PNW released wheat cultivars contain some of identified FHB resistance genes that likely came from 'Norn 10'. Continuation of this project is critical and will distain the genetic mechanism of FHB resistance in sources from Idaho and PNW and develops more adapted lines with resistance to FHB for growers in Idaho and PNW.

Publications

  • Chen, J., Zemetra, R., Souza, E.J., Guttieri, M.J., O'Brien, K., Wheeler, J., Sorensen, L., Clayton, J., Windes, J., and Chen. X. (2010). Registration of UI 'Silver' Wheat. (Ready for submission to JPR).
  • Chen, J., See, D., Liu, Sh., Griffey, C., Zemetra, R., and Windes, J. (2008) Marker-assisted evaluation of FHB resistance in cultivars grown in Pacific Northwest. Manuscript in preparation.


Progress 07/01/08 to 12/31/08

Outputs
OUTPUTS: The research progress was presented as posters at one national and one regional meetings, and as oral talks in one grower tour and at Wheat Coordinated Agricultural Project progress report meeting. PARTICIPANTS: J. Chen, PI, University of Idaho Aberdeen Research & Extension Center, Aberdeen, ID. Oversee marker-assisted selection of FHB resistance in 276 PNW grown lines. D. See, Western Regional Small Grain Genotyping Laboratory, USDA-ARS, Pullman, WA. Conducted marker haplotyping in cooperation with Dr. Jianli Chen. C.R. Hollingsworth3, University of Minnesota Northwest Research & Outreach Center, Crookston, MN. Evaluated 50 spring wheat lines for field FHB resistance in cooperation with Dr. Chen. Dr. Chen provided training on mapping and marker-assisted selection to one visiting scientist from P.R. China TARGET AUDIENCES: U.S. Wheat workers and growers, especially in the PNW region. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
FHB is one of the most destructive diseases of wheat and causes significant reductions in grain yield and quality in the world. The development and deployment of resistant germplasm is an environmentally sustainable method for the control of FHB. FHB is a new wheat disease in Idaho. Evaluation of FHB resistance can be difficult using phenotypic evaluation as the disease nursery can't be set up in Aberdeen, ID. Therefore, we applied marker-assisted evaluation as an alternative of phenotypic evaluation in Aberdeen and conducted phenotypic evaluation through collaborations with Montana State University, University of Minnesota and Virginia Tech. This project firstly identified FHB resistance QTL in adapted varieties and cultivars grown in PNW wheat production areas. These varieties and cultivars will be the primary resources to help growers to fight FHB if it occurs. Continuation of this project is critical and will identify and develop more adapted lines with resistance to FHB.

Publications

  • Chen, J., Hollingsworth, C., See, D., and Windes, J. (2008) Proceeding of the 2008 National Fusarium Head Blight Forum (Abstract),U.S. Wheat & Barley Scab Initiative, Indianapolis, Indiana, December, 2008.