Progress 07/01/07 to 06/30/10
Outputs
OUTPUTS: Activities: Three large experiments were completed evaluating the effects of steroid hormones associated with CAFOs on fathead minnow reproduction and fathead minnow sperm motility. One PhD student, one Vet student and 8 undergraduate students participated in the research his year. Events: Graduate student Zach DeQuattro reported results at the mid-west regionals meeting of SETAC (Soc. Environm. Tox. and Chem.), and the National SETAC conference (Zach won the best student poster award at the national meeting). Terence Barry reported findings at the Toxicology Symposium at the 9th International Congress on the Biology of Fish. Products and Dissemination: the state-of-the-art system being used in our investigations to expose fish to long-term, low-level concentrations of toxicants is now being copied by other researchers. An assay to measure the effects of chemical exposure on sperm motility in fathead minnows has been developed and a paper was recently submitted for publication. PARTICIPANTS: Students: Zach DeQuattro, Patrick Murack, Erica Lundgren, Derek West, Evan Peissig, Ryan Orlund, Mark Richie, Aviv Karasov. UW Faculty: Jamie Schauer, John Parrish. Other Scientists/researchers: Jocelyn Hemming, Dagmara Antkiewicz, Curtis Hedman TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
We discovered that the steroid hormone progesterone may be an important endocrine disrupting chemical. Low environmental levels of this steroid (<300 ng/L) can almost completely inhibit egg production by fathead minnows. The steroid hormone androstenedione has similar effects on fish fecundity. Progesterone has rapid acting effects on fathead minnow sperm motility. This may partial explain the effects of this steroid on reproduction.
Publications
- Zachary A. DeQuattro, Evan John Peissig, Dagmara Antkiewicz, Erica Joy Lundgren, Curtis Hedmen, Derek William West, Terence Patrick Barry. (2011). Effects of Progesterone Exposure on Fathead Minnow (Pimephales promelas) Reproduction. in preparation.
- Patrick J. Murack, John Parrish, Terence P. Barry. (2011), Effects of progesterone on sperm motility in fathead minnow (Pimephales promelas. in preparation.
|
Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: Activities: trained 4 undergraduate students, one Veterinary student, and one PhD student. Conducted experiments to evaluate the effects of steroid hormones associated with concentrated animal feeding operations (CAFOs) on: (1) reproduction of adult fathead minnows, (2) sexual differentiation of larvae, (3) sperm physiology of males, and (4) embryonic development of fish. Events: data presented at national SETAC meeting, New Orleans, Nov. 19-23, 2009. Include conferences, demonstration sites, field days, symposia, workshops, and trainings. Collaborated with scientics at the Wisconsin State Laboratory of Hygiene, and local farmers. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
A change in knowledge occurs when the participant (scientist, trainee, or citizen) learns or becomes aware. Examples of a change in knowledge include: new fundamental or applied knowledge significant enough to be included in a publication; methods and techniques; policy knowledge; improved skills; or increased knowledge of decision-making, life skills, and positive life choices among youth and adults. A change in actions occurs when there is a change in behavior or the participants act upon what they have learned (adoption of techniques and methods or a change in practice). Examples of a change in actions include: application and actual use of fundamental or applied knowledge; adoption of new or improved skills; direct application of information from publications; adoption and use of new methods or improved technologies; use of skills by youth and adults in making informed choices; or adoption of practical policy and use of decision-making knowledge. A change in conditions occurs when a societal condition is changed due to a participant's action. Examples of a change in conditions include: development of the principal discipline(s) of the project or other disciplines; development of human resources; physical, institutional, and information resources that improve infrastructure; technology transfer; management and behavioral changes and adjustments; quantified changes in descriptive statistics (trade balance, export sales, etc.); better and less expensive animal health; changes in conditions (e.g., wages, health care benefits, etc.) of the agricultural workforce; higher productivity in food provision; quantified changes in quality-of-life for youth and adults in rural communities; safer food supply; reduced obesity rates and improved nutrition and health; or higher water quality (e.g., increased water clarity) and a cleaner environment (e.g., measurably reduced pollution).
Publications
- No publications reported this period
|
Progress 01/01/08 to 12/31/08
Outputs
OUTPUTS: We tested the hypothesis that effluent from a cattle-finishing operation in which the animals are administered Synovex implants containing the known endocrine disruptor trenbolone acetate (TNB), would have a negative impact on egg production in fathead minnows. The treatments were continuous 3-week exposure to the following doses of CAFO effluent: 0%, 0.1%, 0.3%, 1%, and 3%. The 100% CAFO effluent was collected from a holding lagoon at the CAFO study site. There was no significant effect of CAFO effluent on the number of eggs produced by fathead minnows. This result is consistent with our observation that hormones associate with the liquid manure are very rapidly degraded. In vitro studies were conducted with mature fathead minnow oocytes to evaluate the impact of pure trenbolone acetate (TNB) on final oocyte maturation. TNB inhibited final oocyte maturation in a dose-dependent manner suggesting that hormones associated with CAFO effluent may have an impact on oocyte maturation without necessarily altering the number of eggs laid per female. Based on our data, we will carefully quantify fertilization percentage in future exposure studies. Trenbolone acetate caused dose-dependant mortalities and developmental abnormalities (e.g., pericardial edema) in developing fathead minnow embryos. Preliminary results indicated that juvenile fish exposed for 3 days to 3% CAFO runoff had significantly higher baseline and stress-induced blood concentrations of cortisol. An advantage of using the stress response as an indicator of contaminant exposure in fish is that it eliminates the inherent variability associated with using reproductive endpoints, and the reliance on reproductively mature individuals. PARTICIPANTS: Co-PIs were Jocelyn Hemming, Martin Schaefer, Steven Geis, and Jamie Schauer. All are associated with the Wisconsin State Laboratory of Hygiene. Graduate student: Sonja Havens. Undergraduate students: Michael Lasecki, Evan Peissig, Dianne O'Brien, Xuejiao Tian, Ryan Olund. Vet student: Patrick Murack. Other collaborators: John Parrish TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
The overall goal is to determine the presence, persistence and biological effects of natural and synthetic hormones that may be released into the environment from CAFOs, and evaluate the effects of different animal waste disposal practices on the fate and activity of these compounds. This research will help to evaluate whether CAFO waste is an important source of endocrine disrupting chemicals in the environment. The results collected to date demonstrated the ecological effects associated with steroid hormones in animal waste from CAFOs on native fish species.
Publications
- No publications reported this period
|
|