Source: MICHIGAN STATE UNIV submitted to NRP
FATE OF ORGANIC POLLUTANTS IN PLANT-BASED REMEDIATION SYSTEMS
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0213392
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Feb 1, 2008
Project End Date
Jan 31, 2013
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
MICHIGAN STATE UNIV
(N/A)
EAST LANSING,MI 48824
Performing Department
BIOSYSTEMS & AGRIC ENGINEERING
Non Technical Summary
Because of prevalent use of pharmaceuticals, personal care products, and pesticides by consumers and in agriculture, organic chemicals are common contaminants in U.S. surface waters. Plant-based systems, including natural and engineered wetlands, vegetative filter strips, and phytoremediation plots, have capabilities to treat waters contaminated with organic chemicals. Research focuses on using controlled-laboratory studies to identify important processes in treatment of organic chemicals in plant-based systems. Laboratory research will also aim to identify native plant species with enhanced capabilities to treat organic chemicals and waters; tissue culture and genetic engineering research may also be used to further enhance capabilities of native plants species with regards to treatment of organic chemicals and growth under unfavorable field conditions. Results from laboratory studies are then to be applied to green-house and field studies to optimize design of plant-based systems that can address full-scale agricultural/urban runoff and waste issues. Additionally, field-scale studies are to be used to assess relative importance of plant-associated processes in environmental fate of organic chemicals.
Animal Health Component
30%
Research Effort Categories
Basic
40%
Applied
30%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1330210107010%
1330210202020%
1330330107010%
1330330202020%
1332420202010%
4030210202010%
4030330202010%
4032420202010%
Goals / Objectives
Research focuses on designing laboratory to field scale systems to assess fate of emerging and agricultural pollutants in plant-based systems, including wetlands, vegetative filter strips, and phytoremediation fields. Particular pollutants of concern include pharmaceuticals, personal care products, and pesticides. These pollutants are introduced to surface water via wastewater effluents, urban stormwater, and agricultural runoff; additionally, these pollutants often coexist with traditional pollutants (e.g., fecal coliforms, suspended solids, nutrients). As plant-based systems can effectively treat many traditional pollutants, it is important to assess the capabilities of natural and engineered ecosystems to treat surface waters containing emerging and agricultural pollutants. Research objectives are to (1) design bench-scale systems in which plant-associated processes can be delineated and quantified with regards to emerging and agricultural pollutants, (2) identify important plant-associated processes for removal of emerging and agricultural pollutants in plant-based systems, (3) identify crucial parameters in plant-associated removal of emerging and agricultural pollutants, (4) optimize design of plant-based systems for treatment of surface waters containing emerging and agricultural pollutants, and (5) engineer plants with enhanced removal capabilities for emerging and agricultural pollutants. Expected outputs include integration of laboratory experimental assessments with field scale applications. Research results are to be integrated into teaching (BE 481 and 482; Ecological Engineering course in development) and will be disseminated via publications in peer-reviewed journals and presentations at national and international conferences.
Project Methods
This research projects aims to integrate laboratory, green-house, and field scale experimentation to yield basic scientific knowledge and the ability to incorporate thus newly discovered knowledge into field-scale applications. On a laboratory and green-house scale, research utilizes both controlled experimentation and mass balance approaches (e.g., C-labeling) to identify and quantify important plant-associated removal processes that contribute to fate of emerging and agricultural pollutants. Tools will be developed at the laboratory and green-house scale to track and quantify important processes in field systems. Analyses are to include separation via liquid chromatography (LC) and gas chromatography (GC), mass spectrometry (e.g., MS, MS/MS, TOF), and UV/Vis spectrometry (e.g., DAD). Research also includes use of tissue culture and genetic engineering to develop plants with enhanced capabilities to metabolize emerging and agricultural pollutants and to grow under unfavorable field conditions. Research activities will be used to train undergraduate and graduate students in experimental design, reactor design, chemical analysis, and result interpretation. Research outputs will be evaluated based on acceptability of results for publication in peer-reviewed literature and ability to apply results to pressing water quality issues at Michigan State University and community.

Progress 02/01/08 to 01/31/13

Outputs
OUTPUTS: Activities: Research activities including experimentation to determine fate of antimicrobials in vegetated soil columns, survey of organic farming practices, and development of methods for botanical insecticide analysis. Events: Research results on fate of antimicrobials in vegetated soil columns were presented at the International Phytotechnologies Conference in December 2009. PARTICIPANTS: Dawn Reinhold (PI): Directed research and supervised student researchers. Niroj Aryal (graduate student): Conducted experiments and analysis on fate of antimicrobials in vegetated soil columns. Vicki Morrone (collaborator, MSU CARRS): Conducted survey on botanical insecticide use by organic farmers. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
Change in knowledge: Research activities contributed to change in knowledge for two undergraduate and one graduate research students. These students learned advanced chemical analysis, experimental design, data analysis, and research communication skills. Change in action: One undergraduate research student is currently applying for graduate schooling in sustainable agriculture, as compared to her initial intent and undergraduate degree in biochemistry.

Publications

  • Aryal, N. and Reinhold, D. M. 2009. Role of plants in migration of antimicrobials. International Phytotechnologies Conference Abstract Book, St. Louis, MO.


Progress 01/01/08 to 12/31/08

Outputs
OUTPUTS: From January 1, 2008 to December 20, 2008, this project has yielded outputs in two areas: fate of antimicrobials from biosolids in vegetated systems and development of probes for wetland studies. The primary experiment completed under this project includes establishment of columns for assessment of fate of antimicrobials from biosolids in soil systems. Switchgrass, pumpkin, and zucchini, were grown in a mixture of biosolids and a silty loam soil and methods were developed for analysis of water samples for antimicrobials. However, two months after start of the experiment, pumpkin and zucchini were infected with powdery mildew. Consequently, student researchers are currently re-designing the experiment based on their previous experiences (to be completed Spring 2009). Additionally, two undergraduate researchers are developing reduction-oxidation potential (ORP) probes for use in wetland treatment studies. Preliminary studies with commercially available probes yielded promising results on ability of aquatic macrophytes to increase ORP of high-strength wastewater, but indicated that commercial probes introduced oxygen to the system because of their size, necessitating development of our own smaller probes. Finally, DR developed a section on organic pollutant characterization and fate for a senior biosystems engineering course. In sum, research has included one MS student and 5 undergraduate students. PARTICIPANTS: Four students have accumulated 1 person month of contribution to this project. Niroj Aryal is a MS student in Biosystems Engineering and is working on the antimicrobial project (Aug - Dec). Catherine Dudgeon, a Junior in Biosystems Engineering, is also researching fate of antimicrobials in vegetated systems (May - Dec). Patrick Triscari, Junior in Biosystems Engineering, worked on the antimicrobial project during the summer (May - Aug). Brad Wardynski is Junior in Biosystems Engineering and is a student researcher on both the antimicrobial and ORP projects (May - Dec). Additional undergraduate students have not accumulated 1 person-month of hours on this project and started in August. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Research yielded the following outcomes and impacts: Change in knowledge: Research dollars have been used to support five undergraduate researchers and one MS students. These students have gained skills in experimental design and execution, analytical chemistry, and presenting research results. Methods for analysis of triclosan and triclocarban in water samples have been developed. Change in actions: Research trainees have adopted good practices with regards to laboratory note keeping and teamwork.

Publications

  • No publications reported this period