Progress 10/01/08 to 09/30/09
Outputs
OUTPUTS: The laboratories at Cornell are addressing components of three objectives in a multi-investigator grant: i) to develop and evaluate molecular hybridization methods such as micro- or macroarrays for identification of the various Potato virus Y (PVY) strains and other viruses in a single, affordable test. (P.I. Keith Perry), ii) to apply improved diagnostic procedures to rapidly identify new strains of PVY, especially those causing tuber necrosis, as they appear. (P.I. Jonathan Whitworth, Stewart Gray), and iii) to evaluate one or more of the improved techniques for use in virus-free seed production and/or shipping point inspections. (P.I. Jonathan Whitworth, Stewart Gray). Our focus has been on developing molecular hybridization methods for arrrays for PVY strain identification. Oligonucleotides for the detection of potato virus Y have been designed and incorporated into a larger macroarray for plant virus detection. This array contains probes for a total of 125 viruses, including multiple potyviruses related to PVY. PARTICIPANTS: Dr. K. L. Perry, Principal Investigator; Dr. S. M. Gray, Principal Investigator; Dr. A. Karasev, Principal Investigator; Dr. J. Lorenzen, Principal Investigator; Dr. J. Whitworth, Principal Investigator. TARGET AUDIENCES: Growers, Extension educators and other extension personnel, Plant disease diagnostians, the US Potato industry. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
Public presentations have been made to grower groups at national meetings (Mello, et al, 2009; Whitworth et al., 2009), including annual industry meetings for potato seed growers. A multi-virus detection array has been designed and implemented. PVY and other viruses have been identified in diagnostic specimens from potato and other crops.
Publications
- Mello, A. F., R. Olarte, S. M. Gray, and K. L. Perry. 2009. Transmission efficiency of Potato virus Y strains PVYO and PVYN-Wi by five aphid species. Phytopathology 99:S83-S83.
- Hu, X. J., T. Meacham, L. Ewing, S. M. Gray, and A. V. Karasev. 2009. A novel recombinant strain of Potato virus Y suggests a new viral genetic determinant of vein necrosis in tobacco. Virus Research 143:68-76.
- Karasev, A. V., T. Meacham, X. Hu, J. Whitworth, S. M. Gray, N. Olsen, and P. Nolte. 2008. Identification of Potato virus Y strains associated with tuber damage during a recent virus outbreak in potato in Idaho. Plant Disease 92:1371-1371.
- Whitworth, J. L., S. M. Gray, A. V. Karasev, and J. H. Lorenzen. 2009. Tuber symptoms are induced in potato by a range of Potato virus Y strains. Phytopathology 99:S141-S141.
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Progress 10/01/07 to 09/30/08
Outputs
OUTPUTS: The objectives for the entire multi-investigator project are: 1. Develop new monoclonal antibodies that can be used for rapid detection and identification of PVY strains that can be utilized on a large scale by certification agencies, researchers, and diagnostic facilities. (P.I. Alex Karasev, Jim Lorenzen). 2. Continue the development of RT-PCR tests for identification of PVY strains that can be used in high-throughput analyses of numerous leaf and tuber samples relatively easily and inexpensively. (P.Is. Jim Crosslin, Alex Karasev, Jim Lorenzen) 3. Develop and evaluate molecular hybridization methods such as micro- or macroarrays for identification of the various PVY strains and other viruses in a single, affordable test. (P.I. Keith Perry). 4. Apply improved diagnostic procedures to rapidly identify new strains of PVY, especially those causing tuber necrosis, as they appear. (P.I. Jonathan Whitworth, Stewart Gray). 5. Evaluate one or more of the improved techniques for use in virus-free seed production and/or shipping point inspections. (P.I. Jonathan Whitworth, Stewart Gray). Our focus has been on objectives 3, 4, and 5. We have been developing molecular hybridization methods for arrrays for PVY strain identification. Oligonucleotides for the detection of potato virus Y have been designed and incorporated into a larger macroarray for plant virus detection (Agindotan and Perry, 2008; Perry et al., 2008). Investigations have also addressed the impact of PVY on storage (Groves, R. L., et al., 2008). PARTICIPANTS: Dr. K. L. Perry, Principal Investigator; Dr. S. M. Gray, Principal Investigator; Dr. A. Karasev, Principal Investigator; Dr. J. Lorenzen, Principal Investigator; Dr. J. Whitworth, Principal Investigator. TARGET AUDIENCES: Growers, Extension educators and other extension personnel, Plant disease diagnostians, the US Potato industry. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Array-based diagnostic technologies have been designed and implemented. Public presentations have been made to grower groups at national meetings (Gray, S. M. et al, 2008; Groves, R. L., et al., 2008), including annual industry meetings for potato seed growers.
Publications
- Agindotan, B., and Perry, K. L. 2008. Macroarray detection of eleven potato-infecting viruses and Potato spindle tuber viroid. Plant Disease 92:730-740.
- Gray, S. M., Karasev, A., Lorenzen, J., Whitworth, J., Nolte, P., and Perry, K. (2008). Emerging diversity in Potato virus Y poses new challenges for the U.S. potato industry. Phytopathology 98: S61.
- Groves, R. L., Charkowski, A. O., Bussan, A. J., and Gray, S. M. (2008). Impact of Potato virus Y on long term storage of potato. Phytopathology 98: S62.
- Perry, K. L., Agindotan, B., Zhang, N., and Smart, C. (2008). A multipathogen detection array for virus, viroid, fungal, and oomycete pathogens of solanaceous crops. Phytopathology 98: S124.
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