Progress 10/01/07 to 09/30/12
Outputs OUTPUTS: Results of this study have been disseminated in several ways. They have been published in peer reviewed publications, and they have been presented at professional conferences. In addition, results were presented at the annual meeting of the Hatch Multistate Research Project, "The Science and Engineering of a Biobased Industry and Economy." PARTICIPANTS: Robert Mark Worden (principal investigator) TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts This research has produced a change in knowledge, which resulted in several publications. In the paper by Lu et al., (2008) entitled, "Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response." The significance of this work is shown by the article's being highlighted in Nanowerk Spotlight, posted September 8, 2008 (http://www.nanowerk.com/spotlight/spotid=7144.php). The following excerpt is from that Nanowerk news article: "Carbon nanomaterials have been extensively used in electroanalysis... To detect biologically derived electronic signals, CNTs are often functionalized with linkers such as proteins and peptides to interface with soluble biologically relevant. Now, for the first time, scientists have tested nanometal decorated graphene (actually graphite nanoplatelets, a thickness of 10 nm would contain approximately 30 graphene sheets, considering an interlayer spacing of 0.335 nm) in biosensor application. As it turned out, this novel biosensor is among the best reported to date in both sensing performance and production cost." In the paper by Hassler et al., (2008) entitled, "Versatile bioelectronic interfaces on flexible non-conductive substrates," novel, nanostructured bioelectronic interfaces were developed that have application for biosensors and biocatalysts. The approach uses a new bench-top method to form bioelectronic interfaces containing a gold film, electron mediator, cofactor, and dehydrogenase enzyme (secondary alcohol dehydrogenase and sorbitol dehydrogenase) on nonconductive substrates such as polystyrene and glass. The method combines layer-by-layer deposition of polyelectrolytes, electroless metal deposition, and directed molecular self-assembly. Cyclic voltammetry, chronoamperometry, field emission x-ray dispersive spectroscopy, scanning electron microscopy, and atomic force microscopy were used to characterize the bioelectronic interfaces. Interfaces formed on flexible polystyrene slides were shown to retain their activity after bending to a radius of curvature of 18 mm, confirming that the approach can be applied on cheap and flexible substrates. The remaining two papers by Jadhav et. al (2008) describe development of novel nanostructured biomimetic interfaces suitable for membrane proteins. These interfaces could have utility to biocatalysis involving membrane-bound enzymes.
Publications
- Lu, J.; Do, I.; Drzal, L. T.; Worden, R. M.; Lee, I. (2008) "Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response," ACS Nano, published online (DOI :10.1021/nn800244k).
- Hassler, B. L.; Amundsen, T. J.; Zeikus, J. G.; Lee, I.; Worden, R. M., (2008), "Versatile bioelectronic interfaces on flexible non-conductive substrates," Biosensors & Bioelectronics 23(10), 1481-1487.
- Jadhav, Sachin R., Zheng, Yi, Garavito, R. Michael, and Worden, R. Mark (2008) "Functional Characterization of PorB class II Porin from Neisseria meningitidis using Tethered Bilayer Lipid Membrane." Biosensors and Bioelectronics, published online (DOI:10.1016/j.bios.2008.07.010)
- Jadhav, S. R.; Sui, D. X.; Garavito, R. M.; Worden, R. M. (2008). "Fabrication of highly insulating tethered bilayer lipid membrane using yeast cell membrane fractions for measuring ion channel activity," Journal of Colloid and Interface Science 322(2), 465-472.
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