Progress 09/01/07 to 08/31/12
Outputs
OUTPUTS: Results have been disseminated to the communities of interest through peer-reviewed journal publications, poster and oral presentations in scientific conferences, and published abstracts. Data from proteomics experiments have been deposited to PRIDE database. PARTICIPANTS: Dr. Attila Karsi is the project director, and Dr. Mark Lawrence serves as the project co-director. Dr. Susan Bridges and Andy Perkins from the Department of Computer Science have been providing support to fulfill the educational outreach component of this project. In CSE 4613/6613 Biocomputing course (fall 2008), students were assigned class projects involving annotation of poorly characterized genes in our microarray data. Dr. Andy Perkins utilized the microarray data in his CSE 6623 Computational Biology course in spring 2010 to teach R based microarray analysis. Dr. Karsi and Perkins have designed class projects for determination of differentially expressed genes using the R environment. Dr. Henry Wang from the College of Veterinary Medicine taught a Functional Genomics course, in which a doctoral student has utilized microarray data from this project as part of her class project. This project has been supporting and providing excellent training opportunities for three PhD (Pradeep Dumpala, Jingjun Lu, and Hossam Abdelhamed), two MS (Hasan Tekedar, Safak Kalindamar), three DVM (Erin Corb, Lauren Phillips, and Katy Fuqua) three undergraduate (Aressa Coley, Rajhkeem Beck, Kimtrele Williams), and one high school (Jeremy Burt) student. TARGET AUDIENCES: This project is directly related to the catfish health research community. Identified virulence mechanisms are expected to better understand pathogenesis of enteric septicemia of catfish and accelerate research on vaccine development for this disease. Therefore, the targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Research undertaken to date revealed the expressed proteins of E. ictaluri, including putative proteins associated with virulence. Microarray experiments and data analysis have been completed, revealing the E. ictaluri gene expression responses to catfish serum. Experiments aiming at understanding the E. ictaluri protein expression responses to catfish serum have been completed. Several differentially regulated proteins have been identified. We developed a mutation technique and produced mutants from differentially expressed genes to understand their functions in E. ictaluri pathogenesis. Differential protein and gene expression analyses of E. ictaluri will lead to a better understanding of E. ictaluri pathogenesis, while mutational analysis of E. ictaluri genes could result in potential live vaccines.
Publications
- Lu J, Lawrence ML, Karsi A. 2012. Virulence and efficacy of the novel Edwardsiella ictaluri mutants in channel catfish. American Society for Microbiology South Central Branch Meeting, Starkville, MS, USA.
- Kalindamar S, Fuqua K, Lu J, M, Lawrence ML, Karsi A. 2012. Identification of the Edwardsiella ictaluri genes causing impaired growth. American Society for Microbiology South Central Branch Meeting, Starkville, MS, USA.
- Dahal N, Abdelhamed H, Karsi A, Lawrence ML. 2012. Determining virulence of Edwardsiella ictaluri mutants using bioluminescence in channel catfish. American Society for Microbiology South Central Branch Meeting, Starkville, MS, USA.
- Fuqua K, Lu J, Abdelhamed H, Banes M, Lawrence ML, Karsi A. 2012. Transposon mutagenesis and identification of mutated genes causing growth deficiency in Edwardsiella ictaluri. Merck-Merial NIH National Veterinary Scholars Symposium 2012. Ft. Collins, CO, USA.
- Abdelhamed H, Lu J, Banes M, Lawrence ML, Karsi A. 2012. Construction and evaluation of an Edwardsiella ictaluri tonB mutant. American Fisheries Society Fish Health Section Annual Meeting, La Crosse, WI, USA.
- Dahal N, Abdelhamed H, Karsi A, Lawrence ML. 2012. Live attenuated Edwardsiella ictaluri vaccines for prevention of enteric septicemia of channel catfish. American Fisheries Society Fish Health Section Annual Meeting, La Crosse, WI, USA.
- Lu J, Abdelhamed H, Perkins A, Banes M, Lawrence ML, Karsi A. 2012. Discovery of Edwardsiella ictaluri virulence genes using functional genomics. American Fisheries Society Fish Health Section Annual Meeting, La Crosse, WI, USA.
- Lawrence ML, Karsi A. 2011. Live attenuated Edwardsiella ictaluri vaccines for prevention of enteric septicemia of catfish. 2011 NIFA Joint Animal Systems PD Workshop. Arlington, VA, USA.
- Lu J, Drnevich J, Perkins A, Banes M, Lawrence ML, Karsi A. 2011. A systematic approach to discovery of Edwardsiella ictaluri virulence genes. American Society for Microbiology South Central Branch Meeting, Monroe, LA, USA.
- Karsi A. 2011. Bacterial diseases of fish. College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
- Abdelhamed H, Lu J, Shaheen A, Abbass A, Lawrence ML, Karsi A. 2012. Construction and evaluation of an Edwardsiella ictaluri fhuC mutant. Veterinary Microbiology. In press. http://dx.doi.org/10.1016/j.vetmic.2012.11.006.
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Progress 09/01/10 to 08/31/11
Outputs
OUTPUTS: Results have been disseminated to the communities of interest through peer-reviewed journal publications, poster and oral presentations in scientific conferences, and published abstracts. Data from proteomics experiments have been deposited to PRIDE database. PARTICIPANTS: Dr. Attila Karsi is the project director, and Dr. Mark Lawrence serves as the project co-director. Drs. Susan Bridges and Andy Perkins from the Department of Computer Science have been providing support to fulfill the educational outreach component of this project. Dr. Bridges teaches CSE 4613/6613 Biocomputing course and she has been collaborating with Dr. Karsi for developing class projects that utilize data from this project. In fall 2008, students were assigned class projects involving annotation of poorly characterized genes in our microarray data. Dr. Andy Perkins utilized the microarray data in his CSE 6623 Computational Biology course in spring 2010 to teach R based microarray analysis. Dr. Karsi and Perkins have designed undergraduate projects for determination of differentially expressed genes using the R environment. Dr. Henry Wang from the College of Veterinary Medicine taught a Functional Genomics course, in which a doctoral student have utilized microarray data from this project as part of her class project. This project has been supporting and providing excellent training opportunities for three PhD (Pradeep Dumpala, Jingjun Lu, and Hossam Abdelhamed), one MS (Hasan Tekedar), and three undergraduate students (Aressa Coley, Rajhkeem Beck, Kimtrele Williams). Pradeep and Jingjun have been working on proteomics and genomics aspects of the project, respectively. Pradeep has successfully completed his PhD and admitted to Penn State College of Medicine as a Veterinary Resident. Jingjun is in 3rd year of her PhD program, and this budget adjustment will allow her to finish her PhD. Hossam has been working on development of E. ictaluri mutants. Aressa and Rajhkeem, minority undergraduate students, have been working on the mutational and bioinformatics analysis aspects of the project. Similarly, Dr. Bianca Garner from Tougaloo College and PDs have established a collaboration to identify E. ictaluri genes that are involved in hemin binding. Dr. Bianca's undergraduate student, Kimtrele Williams, has spent 8 weeks in PDs lab at MSU during the summer of 2010. TARGET AUDIENCES: This project is directly related to the catfish health research community. Identified virulence mechanisms are expected to better understand pathogenesis of enteric septicemia of catfish and accelerate research on vaccine development for this disease. Therefore, the targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Research undertaken to date revealed the expressed proteins of E. ictaluri, including putative proteins associated with virulence. Microarray experiments and data analysis have been completed, revealing the E. ictaluri gene expression responses to catfish serum. Experiments aiming at understanding the E. ictaluri protein expression responses to catfish serum have been completed. Several differentially regulated proteins have been identified. We have initiated mutational analysis of selected differentially expressed genes to understand their functions in E. ictaluri pathogenesis. Differential protein and gene expression analyses of E. ictaluri and functional characterization of differentially expressed genes will lead to a better understanding of E. ictaluri pathogenesis. Moreover, mutational analysis of selected genes could result in potential live attenuated vaccines.
Publications
- Menanteau-Ledouble S, Karsi A, Lawrence ML. 2010. Importance of skin abrasion as a primary site of adhesion for Edwardsiella ictaluri and impact on invasion and systematic infection in channel catfish. Veterinary Microbiology. 2010 Aug 27. [Epub ahead of print].
- Karsi A, Gulsoy N, Corb E, Dumpala PR, Lawrence ML. 2009. High-throughput bioluminescence-based mutant screening strategy for identification of bacterial virulence genes. Applied and Environmental Microbiology. 75: 2166-75.
- Dumpala PR, Lawrence ML, Karsi A. 2009. Proteome analysis of Edwardsiella ictaluri. Proteomics. 9: 1353-1363.
- Published Abstracts (Poster and Talks) Abdelhamed H, Lu J, Banes M, Lawrence ML, Karsi A. 2011. Perturbation of the Edwardsiella ictaluri iron metabolism for live vaccine production. College of Veterinary Medicine Research Day, Mississippi State University, Mississippi State, MS, USA.
- Beck R, Lu J, Karsi A, Perkins A. Homology-based investigation of gene ontology annotations in Edwardsiella ictaluri. Honors Undergraduate Research Program 2011, Mississippi State University, Mississippi State, MS, USA.
- Dumpala PR, Lu J, Lawrence ML, Karsi A. 2011. Identification of differentially regulated proteins of Edwardsiella ictaluri during iron-restriction. 2011 Joint American Fisheries Society Fish Health Section and Western Fish Disease Meeting, Nanaimo, British Columbia, Canada.
- Lu J, Drnevich J, Perkins A, Beck R, Banes M, Lawrence ML, Karsi A. 2011. Effect of host presence and serum killing on global gene expression of Edwardsiella ictaluri. College of Veterinary Medicine Research Day, Mississippi State University, Mississippi State, MS, USA.
- Phillips L, Lu J, Banes M, Lawrence ML, Karsi A. 2011. Genetic modification of the Edwardsiella ictaluri dnaK, arnA, and typA genes for live vaccine development. Merck-Merial NIH National Veterinary Scholars Symposium 2011. Orlando, FL, USA.
- Phillips L, Lu J, Banes M, Lawrence ML, Karsi A. 2011. Molecular vicissitude of Edwardsiella ictaluri for genetic deterrence of enteric septicemia in catfish. College of Veterinary Medicine Research Day, Mississippi State University, Mississippi State, MS, USA.
- Coley A, Lu J, Dumpala PR, Karsi A. 2010. Mutational analysis of Edwardsiella ictaluri lamB, malE, and sdhA genes. Honors Undergraduate Research Program 2010, Mississippi State University, Mississippi State, MS, USA.
- Coley A, Lu J, Karsi A. 2010. Mutational analysis of Edwardsiella ictaluri ispG, and rps2 genes. Summer Undergraduate Research and Mentoring Symposium 2010, Mississippi State University, Mississippi State, MS, USA.
- Williams K, Karsi A, Garner Bianca. 2010. Congo red detection of Edwardsiella ictaluri hemin binding. 2010 Annual Biomedical Research Conference for Minority Students, Charlotte, NC, USA.
- Gulsoy N, Karsi A. 2009. Edwardsiella ictaluri de genetik manipulasyonlar ve canlı aşı potansiyelleri. Fifteenth National Aquaculture Symposium, Rize, Turkey.
- Corb E, Karsi A. 2008. New live attenuated vaccines for enteric septicemia of catfish. Mississippi Veterinary Medical Association Winter Meeting, Mississippi State, MS, USA.
- Karsi A, Lawrence ML. 2008. Application of biophotonics in catfish health. Bio-Inspired Design-Enabling Technologies in the Life and Materials Sciences Conference, Mississippi State, MS, USA.
- Lawrence ML, Corb E, Karsi A. 2008. Use of a high throughput bioluminescence screening method to identify Edwardsiella ictaluri mutants that are susceptible to channel catfish neutrophils and serum. 2008 American Fisheries Society Fish Health Section Annual Meeting, Charlottetown, Prince Edward Island, Canada.
- Menanteau S, Leroux M, Karsi A, Lawrence ML. 2008. Use of green fluorescent protein-labeled Edwardsiella ictaluri to investigate the pathogenesis of enteric septicemia in channel catfish. 33rd Annual Eastern Fish Health Workshop, Atlantic Beach, North Carolina.
- Corb E, Lawrence ML, Karsi A. 2007. Identification of mutated genes of attenuated Edwardsiella ictaluri vaccines. Merck-Merial NIH National Veterinary Scholars Symp., Washington, D.C., USA.
- Dumpala P, Lawrence ML, Karsi A. 2007. Proteome analysis of Edwardsiella ictaluri. College of Veterinary Medicine Research Day, Mississippi State University, Mississippi State, MS, USA.
- Oral Presentations and Invited Lectures Karsi A. 2010. Proteomic and genomic approaches to study fish-pathogen interactions. Jackson State University, Jackson, MS, USA.
- Karsi A. 2009. Use of living colors to study host-pathogen interactions. Student Union, Mississippi State University, Mississippi State, MS, USA.
- Karsi A. 2009. Development of live-attenuated vaccines against bacterial fish diseases. School of Marine Sciences, University of Maine, Orono, ME.
- Karsi A. 2009. Fish and chips. Tougaloo College, Tougaloo, MS, USA.
- Karsi A. 2008. Efficacy of new live attenuated Edwardsiella ictaluri vaccines. 2008 American Fisheries Society Fish Health Section Annual Meeting. Charlottetown, Prince Edward Island, Canada.
- Karsi A. 2008. Use of functional genomics for understanding host-pathogen interactions. Alcorn State University, Alcorn, MS, USA.
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Progress 09/01/09 to 08/31/10
Outputs
OUTPUTS: Results have been disseminated to the communities of interest through peer-reviewed journal publications, poster and oral presentations in scientific conferences, and published abstracts. Data from proteomics experiments have been deposited to PRIDE database. PARTICIPANTS: Dr. Attila Karsi is the project director, and Dr. Mark Lawrence serves as the project co-director. Drs. Susan Bridges and Andy Perkins from the Department of Computer Science have been providing support to fulfill the educational outreach component of this project. Dr. Bridges teaches CSE 4613/6613 Biocomputing course and she has been collaborating with Dr. Karsi for developing class projects that utilize data from this project. In fall 2008, students were assigned class projects involving annotation of poorly characterized genes in our microarray data. Dr. Andy Perkins utilized the microarray data in his CSE 6623 Computational Biology course in spring 2010 to teach R based microarray analysis. Dr. Karsi and Perkins have designed undergraduate projects for determination of differentially expressed genes using the R environment. Dr. Henry Wang from the College of Veterinary Medicine is providing assistance for integration of microarray and proteomics data and identification of target genes using systems biology approaches. In fall 2008, Dr. Wang taught a Functional Genomics course, in which a doctoral student have utilized microarray data from this project as part of her class project. This project has supported and provided excellent training opportunities for two PhD and two undergraduate students. Pradeep Dumpala and Jingjun Lu have been working on proteomics and genomics aspects of the project, respectively. Pradeep Dumpala has successfully completed his PhD and admitted to Penn State College of Medicine as a Veterinary Resident. Aressa Coley, a new minority undergraduate student in our lab, worked on the mutational and virulence analysis aspects of the project. Similarly, Dr. Bianca Garner from Tougaloo College and PDs have established a collaboration to identify E. ictaluri genes that are involved in hemin binding. Dr. Bianca's undergraduate student, Kimtrele Williams, has spent 8 weeks in PDs lab at MSU during the summer of 2010. TARGET AUDIENCES: This project is directly related to the catfish health research community. Identified virulence mechanisms are expected to better understand pathogenesis of enteric septicemia of catfish and accelerate research on vaccine development for this disease. Therefore, the targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Research undertaken to date revealed the expressed proteins of E. ictaluri, including putative proteins associated with virulence. Microarray experiments and data analysis have been completed, revealing the E. ictaluri gene expression responses to catfish serum. Experiments aiming at understanding the E. ictaluri protein expression responses to catfish serum have been completed. Several differentially regulated proteins have been identified. We have initiated mutational analysis of selected differentially expressed genes to understand their functions in E. ictaluri pathogenesis. Differential protein and gene expression analyses of E. ictaluri and functional characterization of differentially expressed genes will lead to a better understanding of E. ictaluri pathogenesis. Moreover, mutational analysis of selected genes could result in potential live attenuated vaccines for the catfish industry.
Publications
- Poster Presentations Coley A, Lu J, Dumpala PR, Karsi A. 2010. Mutational analysis of Edwardsiella ictaluri genes. Honors Undergraduate Research Program, Mississippi State University, Mississippi State, MS, USA.
- Oral Presentations and Invited Lectures Karsi A. 2010. Proteomic and genomic approaches to study fish-pathogen interactions. Jackson State University, Jackson, MS, USA.
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Progress 09/01/08 to 08/31/09
Outputs
OUTPUTS: Results have been disseminated to the communities of interest through peer-reviewed journal publications, poster and oral presentations in scientific conferences, and published abstracts. Data from proteomics experiments have been deposited to PRIDE database and microarray data will soon be deposited to NCBI's GEO database. PARTICIPANTS: Dr. Attila Karsi is the project director and Dr. Mark Lawrence serves as the project co-director. Dr. Susan Bridges from the Department of Computer Science serves as the senior personnel on this project. She has been providing support to fulfill the educational outreach component of this project. Dr. Bridges teaches CSE 4613/6613 BioComputing course and she has been collaborating with Dr. Karsi for developing class projects that utilize data from this project. In fall 2008, students were assigned class projects involving annotation of poorly characterized genes in our microarray data. Dr. Karsi has established a new collaboration with Dr. Andy Perkins from the Department of Computer Science. He has been contributing to this project through utilization of microarray data in his CSE 6623 Computational Biology course. Dr. Karsi and Perkins have designed projects for determination of differentially expressed genes using the R environment in the class of spring 2010. Dr. Karsi has also established a new collaboration with Dr. Henry Wang from the College of Veterinary Medicine. Dr. Wang is providing assistance for integration of microarray and proteomics data and identification of target genes using systems biology approaches. Dr. Wang also helps accomplish educational aspect of the project. In fall 2008, Dr. Wang taught a Functional Genomics course, in which doctoral students have worked on microarray data analysis and explored ways to combine proteomics and gene expression data. This project has supported and provided excellent training opportunities for two Ph.D. level graduate students, Pradeep Dumpala and Jingjun Lu who are working on proteomics and genomics aspects of the project, respectively. In addition, a new minority undergraduate student is being recruited for the remainder of the project to work on the mutational and virulence analysis aspects of the project. TARGET AUDIENCES: This project is directly related to the catfish health research community. Identified virulence mechanisms are expected to better understand pathogenesis of enteric septicemia of catfish and accelerate research on vaccine development for this disease. Therefore, the targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Research undertaken to date revealed the expressed proteins of E. ictaluri, including putative proteins associated with virulence. Microarray experiments have been completed and data analysis to determine the E. ictaluri gene expression responses to catfish serum is underway. Experiments aiming at understanding the E. ictaluri protein expression responses to catfish serum and iron restriction have been completed. Several differentially regulated proteins have been identified and knock out studies are underway. We expect that differential protein and gene expression analyses of E. ictaluri will lead to a better understanding of E. ictaluri pathogenesis. Moreover, understanding mechanisms of E. ictaluri pathogenesis during the early stages of infection will allow development of new strategies to prevent the disease before systemic infection is established. Finally, this study could result in the development of several attenuated vaccines to reduce the devastating effect of this pathogen on the catfish industry.
Publications
- Refereed Research Articles 1. Karsi A, Gulsoy N, Corb E, Dumpala PR, Lawrence ML. 2009. High-throughput bioluminescence-based mutant screening strategy for identification of bacterial virulence genes. Applied and Environmental Microbiology. 75: 2166-75. 2. Dumpala PR, Lawrence ML, Karsi A. 2009. Proteome analysis of Edwardsiella ictaluri. Proteomics. 9: 1353-1363.
- Poster Presentations 1. Karsi A, Lawrence ML. 2008. Application of Biophotonics in Catfish Health. Bio-Inspired Design-Enabling technologies in the life and materials sciences conference. Mississippi State University, Mississippi State, MS, USA. 2. Dumpala P, Lawrence ML, Karsi A. 2007. Proteome analysis of Edwardsiella ictaluri. College of Veterinary Medicine Research Day, Mississippi State University, Mississippi State, MS, USA. 3. Corb E, Lawrence ML, Karsi A. 2007. Identification of mutated genes of attenuated Edwardsiella ictaluri vaccines. Merck-Merial NIH National Veterinary Scholars Symposium 2007, Washington, D.C., USA.
- Published Abstracts 1. Gulsoy N, Karsi A. 2009. Edwardsiella ictaluri de genetik manipulasyonlar ve canlı aşı potansiyelleri. 15. Ulusal Su Urunleri Sempozyumu, Rize, Turkey. 2. Karsi A, Gulsoy N, Dumpala PR, Greenway T, Wise DJ, Lawrence ML. 2008. Efficacy of new live attenuated Edwardsiella ictaluri vaccines. 2008 American Fisheries Society Fish Health Section Annual Meeting. Charlottetown, Prince Edward Island, Canada. 3. Lawrence ML, Corb E, Karsi A. 2008. Use of a high throughput bioluminescence screening method to identify Edwardsiella ictaluri mutants that are susceptible to channel catfish neutrophils and serum. 2008 American Fisheries Society Fish Health Section Annual Meeting. Charlottetown, Prince Edward Island, Canada. 4. Corb E, Karsi A. 2008. New live attenuated vaccines for enteric septicemia of catfish. Mississippi Veterinary Medical Association Winter Meeting, Mississippi State, MS, USA.
- Oral Presentations and Invited Lectures 1. Karsi A. 2009. Use of living colors to study host-pathogen interactions. Student Union, Mississippi State University, Mississippi State, MS, USA. URM student recruitment presentation given to 25 undergraduates. 2. Karsi A. 2009. Development of live-attenuated vaccines against bacterial fish diseases. School of Marine Sciences, University of Maine, Orono, ME. 3. Karsi A. 2009. Fish and chips. Tougaloo College, Tougaloo, MS, USA. 4. Karsi A. 2008. Efficacy of new live attenuated Edwardsiella ictaluri vaccines. 2008 American Fisheries Society Fish Health Section Annual Meeting. Charlottetown, Prince Edward Island, Canada. 5. Karsi A. 2008. Use of functional genomics for understanding host-pathogen interactions. Alcorn State University, Alcorn, MS, USA.
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Progress 09/01/07 to 08/31/08
Outputs
OUTPUTS: Results have been disseminated to the communities of interest through a peer-reviewed publication and raw data from proteomics experiments have been deposited in a public database. PARTICIPANTS: Dr. Attila Karsi is the project director and Dr. Mark Lawrence serves as the project co-director. This project has supported and provided training for two Ph.D. level graduate students, Pradeep Dumpala and Jingjun Lu who are working on proteomics and genomics aspects of the project, respectively. TARGET AUDIENCES: This project is directly related to the catfish health research community. Identified virulence mechanisms are expected to better understand pathogenesis of enteric septicemia of catfish and accelerate research on vaccine development for this disease. Therefore, the targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
We expect that global gene expression profiles of E. ictaluri will lead to a better understanding of E. ictaluri pathogenesis. Moreover, understanding mechanisms of E. ictaluri pathogenesis during the early stages of infection will allow development of new strategies to prevent the disease before systemic infection is established. Finally, this study could result in the development of an attenuated vaccine to combat and reduce the devastating effect of this pathogen on the catfish industry.
Publications
- Dumpala PR, Lawrence ML, Karsi A. 2009. Proteome analysis of Edwardsiella ictaluri. Proteomics. In press.
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