Progress 10/01/07 to 09/30/11
Outputs
OUTPUTS: The major goal of the project is to clone a gene for yield related traits in maize. The gene is on chromosome 1 of maize. During the summer of 2011, we confirmed that the gene lies in a relatively small interval of about 3000 bp. We had initially planned to finished the mapping in summer 2010, however the distribution of our originally 40 recombinant chromosomes was such that the gene could not be completely mapped. Thus in Summer 2010, we isolated an additional 166 recombinant chromosomes which were used to mapping the gene in Summer 2011. We trained two undergraduate student (Erik Nyse and Joel Wippelfurth) who worked on this project. Data from this work has been (or will be) deposited in the PANZEA database and is available on line at www.panzea.org. Tony Studer, the student supported by this project, attended the Maize Genetics Conference in March 2011 and discussed our work with other maize geneticists in that context. We plan to submit the final publication identifying gt1 as the causal gene in 2012. PARTICIPANTS: Doebley, John. The principal investigator who oversaw the project and worked on preparation of publications. Wills, David. Postdoctoral fellow who has assisted with this project. Shannon, Laura. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Studer, Anthony. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Weber, Allison. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. TARGET AUDIENCES: The principal target audience is research scientist working on crop improvement in the broadest sense. This group would include graduate students, research professors, research scientist is public agencies such as the USDA, and research scientists in industry. PROJECT MODIFICATIONS: none
Impacts
The main outcome for the past year was the finally mapping of the gene to a 3000 bp portion of chromosome 1. This work identified gt1 as the gene that controls the yield related traits and showed that part of the gene that affects gene function is in the upstream regulatory region. We have also shown that maize and teosinte differ in the level of gt1 expression such that the teosinte allele is expressed at a higher level.
Publications
- Whipple, C. J., T. H. Kebrom, A. L. Weber, F. Yang, D. H. Hall, R. B. Meeley, R. J. Schmidt, J. Doebley, T. P. Brutnell and D. P. Jackson. 2011. Grassy tillers1 integrates light signals to control apical dominance in maize. Proc. Natl. Acad. Sci. USA108: E506-E512.
- Quijada, P., L. M. Shannon, J. C. Glaubitz, A. J. Studer, and J. Doebley. 2009. Characterization of a major maize domestication QTL on the short arm of chromosome 1. Maydica 54:401-408.
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: Outputs are activities, events, services, and products. There are no services and no events as part of this project. The major goal of the project is to clone a gene for yield related traits in maize. The gene is on chromosome 1 of maize. During the summer of 2010, we confirmed that the gene lies in a relatively small interval of about 1.5 Mb. We isolated over 206 recombinant chromosomes in this region. We had initially planned to finished the mapping in summer 2010, however the distribution of our originally 40 recombinant chromosomes was such that the gene could not be completely mapped. Thus in Summer 2010, we isolated an additional 166 recombinant chromosomes which will be used to map the gene in Summer 2011. We trained two undergraduate student (Erik Nyse and Joel Wippelfurth) who worked in this project. Data from this work has been deposited in the PANZEA database and is available on line at www.panzea.org. Tony Studer, the student supported by this project, attended the Maize Genetics Conference in March 2010 and discussed our work with other maize geneticists in that context. PARTICIPANTS: Doebley, John. The principal investigator who oversaw the project and worked on preparation of publications. Shannon, Laura. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Studer, Anthony. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Weber, Allison. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. TARGET AUDIENCES: The principal target audience is research scientists working on crop improvement in the broadest sense. This group would include graduate students, research professors, research scientist is public agencies such as the USDA, and research scientists in industry. PROJECT MODIFICATIONS: In 2010, my lab was awarded a new 3 year NSF grant to expand this relative small project. Under this new funding, we will be looking at a class of genes that were the targets of selection during maize domestication and determining the phenotypes that they affect.
Impacts
OUTCOMES. The main outcome for the past year is the isolation of a large collection of recombinant chromosomes mentioned about under outputs. These are the key resource required to clone the QTL. These materials will be assayed during the Summer of 2011.
Publications
- Clinton J. Whipple, Ph.D.;Tesfamichael H. Kebrom;Allison L. Weber;Fang Yang;Darren H. Hall;Robert B. Meeley;Robert J. Schmidt;John Doebley;Thomas P. Brutnell;David P. Jackson. 2011. Grassy tillers1 integrates light signals to control apical dominance in maize. PLoS Biology (in review).
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Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: The major goal of the project is to clone a gene for yield related traits in maize. The gene is on chromosome 1 of maize. During the summer of 2009, we mapped the gene to a relatively small interval of about 1 Mb and isolated about 40 recombinant chromosomes in that region. These recombinant chromosomes will be used to finish the mapping or cloning of the gene during the summer of 2010. We trained one undergraduate student (Erik Nyse) who worked on this project. Data from this work has been deposited in the PANZEA database and is available on line at www.panzea.org. Tony Studer, the student supported by this project, attended the Maize Genetics Conference in March 2009 and discussed our work with other maize geneticists in that context. PARTICIPANTS: Doebley, John. The principal investigator who oversaw the project and worked on preparation of publications. Shannon, Laura. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Studer, Anthony. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Weber, Allison. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. TARGET AUDIENCES: The principal target audience are research scientists working on crop improvement in the broadest sense. This group would include graduate students, research professors, research scientist is public agencies such as the USDA, and research scientists in industry. PROJECT MODIFICATIONS: none
Impacts
The main outcome for the past year is the isolation of the recombinant chromosomes mentioned about under outputs. These are the key resource required to clone the QTL. These materials will be assayed during the Summer of 2010.
Publications
- Quijada, P., L. M. Shannon, J. C. Glaubitz, A. J. Studer, and J. Doebley. 2010. Characterization of a major maize domestication QTL on the short arm of chromosome 1. Maydica (in press).
- Weber, A. L., Q. Zhao, M. D. McMullen, J. F. Doebley. 2009. Using association mapping in teosinte to investigate the function of maize selection-candidate genes. Plos One 4:1-6, e8227.
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Progress 01/01/08 to 12/31/08
Outputs
OUTPUTS: Outputs are activities, events, services, and products. We did conduct experiments and analyze data collected as part of these experiments. The experiments involved fine mapping QTL with the goal of positionally cloning the genes that underly the QTL. Specifically, our initial proposal was the positional cloning of a QTL designated ed1.1 that controls ear diameter in maize. With new NSF support, we are expanding this goal to positionall clone up to 4 QTL. This past year we geneotyped and selected the stocks for cloning of these 4 QTL. The advanced stocks for this effort are currently being grown in our winter nursery. Graduate student, Laura Shannon, continues to perform joint QTL mapping to narrow down the genomic locations of these QTL. PARTICIPANTS: Doebley, John. The principal investigator oversees the project and works on preparation of publications; Shannon, Laura. Graduated student supported by the project who will collect and analyze all and write drafts of the publications; Studer, Anthony. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Weber, Allison. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. TARGET AUDIENCES: The principal target audience is research scientists working on crop improvement in the broadest sense. This group would include graduate students, research professors, research scientist is public agencies such as the USDA, and research scientists in industry. PROJECT MODIFICATIONS: My lab has just been award a 4 year NSF grant to expand this relative small project. As such, we will seek to clone 4 QTL rather than just one. The overall approach will be the same, although we will use a modified set of genetic stock that will be developed with the NSF support.
Impacts
This 4 year project is just beginning its second year. We have no specific findings except to report that the graduate student, Laura Shannon, has been successful in localizing multiple QTL to small intervals and we have selected genetic stocks for fine-mapping the QTL.
Publications
- Weber, A. L., W. H. Briggs, J. Rucker, B. M. Baltazar, J. J. Sanchez-Gonzalez, P. Feng, E. S. Buckler and J. Doebley. 2008. The Genetic Architecture of Complex Traits in Teosinte (Zea mays ssp. parviglumis): New Evidence from Association Mapping. Genetics 180:1221-1232.
- Zhao, Q., A.-C. Thuillet, N. K Uhlmann, A. Weber, J. A. Rafalski, S. M. Allen, S. Tingey, and J. Doebley. 2008. The role of regulatory genes during maize domestication: evidence from nucleotide polymorphism and gene expression. Genetics 178: 2133-2143.
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Progress 01/01/07 to 12/31/07
Outputs
OUTPUTS: Outputs are activities, events, services, and products. There are no services and no events as part of this project. We did conduct experiments and analyze data collected as part of these experiments. The experiments involved fine mapping QTL with the goal of positionally cloning the genes that underly the QTL. Spcifically, we are working on the postional cloning of a QTL designated ed1.1 that controls ear diameter in maize. This past year we genotype the lines possessing alternate alleles for ed1.1 and made crosses between the genetic stock carrying these alleles. These are the first steps toward our goal. We are simultaneously working to positionally clone additional QTL affecting culm diameter and ear morphology.
PARTICIPANTS: Doebley, John. The principal investigator who oversaw the project and worked on preparation of publications. Shannon, Laura. Graduated student supported by the project who will collect and analyze all and write drafts of the publications. Studer, Anthony. Graduated student supported by the project who will collect and analyze all and write drafts of the publications.
TARGET AUDIENCES: The principal target audience is research scientist working on crop improvement in the broadest sense. This group would include graduate students, research professors, researcg scientist is public agencies such as the USDA, and research scientists in industry.
PROJECT MODIFICATIONS: none
Impacts
This is a new project and we have no specific findings at this time. The project has only been funded for three months at this point.
Publications
- No publications reported this period
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