Recipient Organization
MINNESOTA STATE UNIVERSITY, MANKATO
228 WIECKING CTR
MANKATO,MN 56001-6062
Performing Department
(N/A)
Non Technical Summary
1. Damage to certain essential chemicals in our bodies has been suggested to be related to age-related development of some cancers, development of cardiovascular disease, cataractogenesis, atherosclerosis, rheumatoid arthritis, diabetes, and neurodegenerative diseases including Alzheimer's disease. Compounds call antioxidants may help to reduce the damage. 2. Antibiotics that make their way into the soil can also appear in our food supply and/or contribute to an increase in antibiotic resistant organisms. 3. Light can make oxygen very reactive (photochemical processes). These photochemical processes strongly influence the active lifetime, bioavailability, degradation products, and ultimate fate of agrochemicals that are applied to soil surfaces. The purpose of this project is to purchase a liquid chromatography system with mass spectral capabilities.
Animal Health Component
(N/A)
Research Effort Categories
Basic
100%
Applied
(N/A)
Developmental
(N/A)
Goals / Objectives
The objective of this project is the purchase of a Shimadzu liquid chromatography system with mass spectral capabilities (LCMS)that will be housed in the Chemistry and Geology Department at Minnesota State University, Mankato.
Project Methods
The measurements of biomarkers of oxidative damage to proteins and DNA as well as lipids using liquid chromatography linked to mass spectroscopy (LCMS) will be used to establish the role of antioxidants in protecting against oxidative damage. One compound of particular interest with respect to oxidative damage to DNA is 8-hydroxydeoxyguanosine (8-OHdG). Determination of the concentration of urinary 8-OHdG will be accomplished by reverse phase HPLC with tandem mass spectrometric detection. This method requires comparatively little sample clean up while offering both measurement and confirmatory identification of 8-OHdG. 5-(hydroxymethyl)furfural (HMF) is formed as a product in the nonenzymatic browning reactions that result when food containing reducing sugars and amino acids are heated. HMF is also present in many foods including milk, fruit juice and tomato products. An LCMS base approach will be used to evaluate the mutagenicity of HMF and other metabolites derived
from sugars. After standards of the proposed target HMF adducts are chemically synthesized, these synthetic standards will be fully characterized by nuclear magnetic resonance (NMR) spectroscopy, ultraviolet (UV) spectroscopy, high pressure liquid chromatography (HPLC) retention time and mass spectral (MS) fragmentation patterns. The standards will then be used for comparison with biological isolates to determine if the proposed adducts actually form when DNA is exposed to HMF. There is scant information concerning the potentially important role of clays in promoting antibiotic removal in soil systems. Knowledge of the chemical factors controlling these clay-facilitated removal and degradation pathways will aid in predicting the mobility and fate of antibiotics in soil environments. LCMS will be used to quantify the removal of two common veterinary and human antibiotics with similar chemical structures, erythromycin and clarithromycin, by clay minerals. The LC system and size
exclusion chromatography with fluorescence detection will be used on organic matter extracted from local agricultural soils in order to ascertain any relevant relationships between the observed organic matter fluorescence properties and the organic matter source material. These studies will lead to more detailed mechanistic studies of the photochemical degradation of selected agrochemicals, using the LCMS system to identify and quantify the observed photoproducts. These studies will allow us to identify predictive features of the organic matter fluorescence spectra that can be used to improve the accuracy of models that are used to predict the fate and transport of agrochemicals applied to the soil surface.