Source: KANSAS STATE UNIV submitted to
GREAT PLAINS DIAGNOSTIC NETWORK
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0210737
Grant No.
2007-37620-18152
Project No.
KS600644
Proposal No.
2010-03573
Multistate No.
(N/A)
Program Code
AA-G
Project Start Date
Jun 1, 2007
Project End Date
May 31, 2012
Grant Year
2011
Project Director
Stack, J. P.
Recipient Organization
KANSAS STATE UNIV
(N/A)
MANHATTAN,KS 66506
Performing Department
PLANT PATHOLOGY
Non Technical Summary
GPDN supported the following national plant diagnostic response efforts since 2003: citrus greening, Ralstonia solanacearum r3b2, pink hibiscus mealybug, Plum Pox Virus, soybean aphid, Asian Longhorned beetle, Asian soybean rust, Pale Cyst Nematode, Phytophthora ramorum blight. GPDN developed and implemented several programs for diagnostics, communications, and training. Kansas State University serves as the regional center for GPDN and contributes to the NPDN database at Purdue University. Infrastructure of the regional and state diagnostic labs was measurably enhanced. Labs were equipped with an array of technologies including, web-enabled microscopy and advanced diagnostics technology including standard and Real-Time PCR. GPDN labs provided triage and preliminary diagnoses for the response to the shipment of P. ramorum contaminated plant materials across the U.S. from 2004-2006. Triage by NPDN labs resulted in an approximate 100-fold reduction in samples shipped to the APHIS PPQ CPHST expert lab for confirmatory diagnoses. GPDN implemented a secure communications system for the timely transfer of sensitive information during outbreaks. GPDN implemented an exercise program to train those involved in outbreak response. All GPDN states have conducted exercises involving more than 40 individuals per exercise. After action reports were prepared and analyzed for lessons learned.
Animal Health Component
(N/A)
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2112499106033%
2122499106033%
2132499106034%
Goals / Objectives
1. Provide Regional Coordination and Governance for the Great Plains Diagnostic Network (GPDN) 2. Implement the First Detector Education and Training Program for GPDN 3. Maintain and Contribute to the Functional Data Management Systems and Networks 4. Maintain and Evolve Functioning Diagnostic Systems for GPDN 5. Support the NPDN Data Analysis for Event Detection Project 6. Develop Response Activation and Functioning Decision Support Mechanisms
Project Methods
GPDN will participate in the annual NPDN planning meeting and help develop the annual plan of work and report of accomplishments. GPDN will participate in operations committee and issue-based meetings. Communications among GPDN, LGU administration, IPM centers, and other key groups will be improved. GPDN will keep the general public and key groups informed about the national network and its service to agriculture. A dependable information security system for the PDIS communications system will be implemented, including regular testing of the replicated server system at Cornell University. GPDN regional center will help coordinate and execute the NPDN national meeting and will coordinate and conduct an annual regional planning meeting. The regional meeting will address administrative issues and conduct professional development programs. GPDN will contribute to writing the final report for the cooperative agreement and to draft a proposal for a new cooperative agreement. GPDN states will develop and implement first detector education and training programs in collaboration with the national training and education committee. Further efforts will be made to collaborate with the regional IPM centers and EDEN in the development and distribution of training materials regarding high risk pests and pathogens. Specifically, a series of educational materials regarding insect vectors of plant pathogens important to Great Plains agriculture will be initiated More effort will be given to the GPDN portal system to improve the content. A notification system will be devised and implemented to alert subscribers to content updates and issue alerts. The PDIS-based ag alert project implemented in Montana will be evaluated for implementation in additional GPDN states. Improvements to the reporting and mapping function of PDIS will be made. GPDN will develop and implement a secure communications exercise program for GPDN states that will ensure familiarity with the software. GPDN regional diagnosticians will participate in training opportunities provided by APHIS expert labs regarding high risk pests and pathogens, including select agents. A program will be developed to transfer that training to GPDN member state diagnosticians as appropriate. The GPDN regional lab will continue to provide diagnostic support to expert labs as well as state and regional labs within NPDN. The regional center will continue to promote interaction among states and provide a forum for the facilitation of collaboration. GPDN will contribute to the formulation of a national plan for the accreditation and certification of diagnostic laboratories. GPDN will work with individual states to support surge issues for soybean rust, Phytophthora ramorum, pink hibiscus mealy bug, or other high risk pests and pathogens. Regional efforts in Entomology training and diagnostics will be increased. GPDN will support the national efforts in the areas of data analysis and event detection by providing financial and intellectual support to the WPDN program at Oregon State University. A data reporting module is under development for the PDIS database system that will permit limited data analysis capability.

Progress 06/01/07 to 05/31/12

Outputs
OUTPUTS: Coordination/governance mechanisms for regional and national networks. As in the past year, due to budget reductions, GPDN did not convene a regional meeting during 2012. Regional conference calls were convened to manage administrative issues, review policies, develop plans of work, and to discuss emerging issues. The GPDN Director and Associate Director developed and deployed the request for funding from GPDN member states and reviewed the submissions for consistency with the NPDN mission and project planning matrix. Annual reports of accomplishments were solicited, received, and reviewed. GPDN continued to provide leadership for the National Outbreak Preparedness Exercise Program. Regional staff participated in all relevant network meetings. GPDN continued to provide the leadership and oversight for the Plant Diagnostic Information System (PDIS) in use in over 30 states. GPDN members (diagnosticians and extension specialists) presented and published material focusing on general plant pathology, diagnosing plant problems, GPDN and NPDN, pest emergencies, fungicide and pesticide resistance management, and specific plant disease and pest issues. GPDN members conducted many training sessions on an array of pests and diseases for First Detectors, although at a reduced rate due to budget reductions. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Attendees included individuals from grower organizations, crop consultants, master gardeners, first detectors, horticultural society members and plant pathologists/ entomologists. Data systems and networking. GPDN continued to provide leadership and oversight for the Plant Diagnostic Information System (PDIS) in use in over 30 states. The GPDN Regional Center Staff provided oversight for the process to develop, test, and implement the new applications-based version of PDIS. The PDIS team developed the code and provided technical support during the implementation of the new version. GPDN continues to support the overall Network IT goals and action items. Functioning diagnostic systems. Diagnostics - GPDN state labs continued to upgrade, where possible, their capabilities through the purchase of advanced diagnostic technology and to implement appropriate equipment maintenance programs that include scheduled certifications and record keeping. Progress was made toward the goal for all GPDN labs to have molecular diagnostic capability. Diagnosticians from several GPDN states received advanced training through an array of formats from hands-on workshops to symposia. GPDN members attended training programs sponsored by NPDN and by USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory. These training sessions focused on molecular and morphological identification techniques for high consequence pathogens. The training sessions were conducted by USDA-APHIS experts at the federal laboratory in Beltsville, Maryland. PARTICIPANTS: Jim Stack, Ned Tisserat, Mary Burrows, Amy Timmerman, Sam Merkel, Rick Grantham, Larry Osborne, Ron French, Charlie Rush, Gary Franc TARGET AUDIENCES: Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, Wyoming PROJECT MODIFICATIONS: A new annual plan of work was developed in compliance with the NPDN Strategic Plan and Project Planning Matrix.

Impacts
A change in knowledge: Diagnosticians - Diagnosticians throughout the Great Plains Region increased their knowledge of the diagnosis of high consequence plant pathogens and insect pests. This was accomplished through participation in lecture-based seminars delivered in person or over the web and by participation in hands-on laboratory workshops. Diagnosticians from GPDN states completed diagnostic training offered by USDA APHIS PPQ CPHST. The diagnosticians from these states are now prepared to conduct those protocols in their individual state diagnostic laboratories. First detectors - Across the nine-state Great Pains Region, hundreds of presentations were delivered to potential first detectors to prepare them to identify disease and pest outbreaks and to report them to appropriate authorities. A change in actions: All nine GPDN member state diagnostic laboratories implemented laboratory equipment calibration and maintenance plans to keep critical laboratory equipment in the running condition necessary to generate reliable and repeatable results. All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN National Data Repository for analysis by epidemiologists for the early detection of outbreaks.

Publications

  • No publications reported this period


Progress 06/01/11 to 05/31/12

Outputs
OUTPUTS: Budget reductions and the NPDN National meeting, GPDN did not convene a regional meeting this year. Regional conference calls were convened to manage administrative issues, review policies, develop plans of work, and to discuss emerging issues. GPDN continued to provide leadership for the National Outbreak Preparedness Exercise Program. Regional staff participated in all relevant network meetings. GPDN continued to provide leadership and oversight for the Plant Diagnostic Information System (PDIS) in use in over 30 states. GPDN members presented and published material focusing on general plant pathology, diagnosing plant problems, GPDN and NPDN, pest emergencies, fungicide and pesticide resistance management, and specific plant disease and pest issues such as wheat stem rust strain Ug99, Africanized Honey Bee, 1000 cankers disease, and Triticum mosaic virus. Members conducted many training sessions on an array of pests and diseases for First Detectors. First Detector Expanded Awareness presentations occurred during sessions at which members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Attendees included individuals from grower organizations, crop consultants, master gardeners, first detectors, horticultural society members and plant pathologists/ entomologists. We continued to provide leadership and oversight for the PDIS in use in over 30 states. The GPDN Regional Center Staff provided oversight for the process to develop, test, and implement the new version of PDIS. The PDIS team developed the code and provided technical support during the implementation of the new version. GPDN continues to support the overall Network IT goals and action items. GPDN state labs continued to upgrade their capabilities through the purchase of advanced diagnostic technology and to implement appropriate equipment maintenance programs that include scheduled certifications and record keeping. Progress was made toward the goal for all GPDN labs to have molecular diagnostic capability. Diagnosticians from all nine GPDN states received some advanced training through an array of formats from hands-on workshops to symposia. The training sessions focused on molecular and morphological identification techniques for high consequence pathogens. Diagnosticians from all nine GPDN states received some advanced training through an array of formats from hands-on workshops to symposia. Data upload - All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN for analysis by epidemiologists for the early detection of outbreaks: CO: 2258, KS: 2017, MT: 1553, NE: 3721, ND: 824, OK: 3487, SD: 1977, TX: 796, WY: 376. The number of samples from GPDN member states received by the NPDN totaled 17,009 samples submitted. All nine GPDN states participated in the GPDN Regional Wheat Virus Survey; 2010 was the third year of the survey. No new viruses were detected. The following viruses were detected: Wheat Streak Mosaic Virus, Barley Yellow Dwarf Mosaic Viruses - PAV & RPV, the virus that causes the High Plains Disease, Triticum mosaic virus, and Wheat Soilborne Mosaic Virus. PARTICIPANTS: Jim Stack, Ned Tisserat, Mary Burrows, Amy Timmerman, Sam Merkel, Rick Grantham, Larry Osborne, Ron French, Charlie Rush, Gary Franc TARGET AUDIENCES: Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, Wyoming PROJECT MODIFICATIONS: A new annual plan of work was developed in compliance with the NPDN Strategic Plan and Project Planning Matrix.

Impacts
Diagnosticians throughout the Great Plains Region increased their knowledge of the diagnosis of high consequence plant pathogens and insect pests. This was accomplished through participation in lecture-based seminars delivered in person or over the web and by participation in hands-on laboratory workshops. Diagnosticians from GPDN states completed diagnostic training offered by USDA APHIS PPQ CPHST. The diagnosticians from these states are now prepared to conduct those protocols in their individual state diagnostic laboratories. First detectors - Across the nine-state Great Pains Region, over 400 presentations were delivered to potential first detectors to prepare them to identify disease and pest outbreaks and to report them to appropriate authorities. All nine GPDN member state diagnostic laboratories implemented laboratory equipment calibration and maintenance plans to keep critical laboratory equipment in the running condition necessary to generate reliable and repeatable results. All GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN for analysis by epidemiologists for the early detection of outbreaks. The regional Center organized a GPDN Annual Meeting for all member states at which annual plans of work were developed, open exchange of challenges faced were discussed, and solutions were derived. Webinar Series - In addition, Montana State University hosted a GPDN webinar series from January through April 2010. Eleven speakers from around the region and one from the Northeast Region presented nine weekly seminars on emerging diseases and emerging insect pests. These Adobe Connect webinars were very well attended with excellent question and answer sessions. The GPDN Regional Center provides guidance and management of the funding process to ensure that GPDN member states completed necessary forms and adhered to guidelines and met all requirements. The GPDN Regional Center provided oversight to ensure that the minimum expectations for funding were met including, participation at the annual regional meeting, participation in diagnostic training workshops, end of year reporting, and the involvement of both pathologists and entomologists in diagnostics and training programs. Those added responsibilities include regional IT support, regional diagnostic support, regional coordination and management of the outbreak preparedness exercise program, and administrative management of the regional Network. GPDN provided leadership for the PDIS diagnostic laboratory software system used by many states in the national Network. All of the seminars presented in the GPDN webinar series are archived on the GPDN portal to facilitate asynchronous access to these valuable educational resources. Extension publications for first detectors were produced and disseminated. The mission and function of the diagnostic network was explained in presentations at national and international meetings.

Publications

  • Stack, J.P. 2010. Diagnostic networks for plant biosecurity. In, Knowledge and technology transfer for plant pathology. Hardwick, N. Gullino, M.L. eds. 123 p. Springer, Dordrecht.
  • Stack, J.P., Suffert, F., and Gullino, M.L. 2010. Bioterrorism: a threat to plant biosecurity In, The role of plant pathology in food safety and food security, Strange R.N., Gullino M.L. eds. 155 p. Springer, Dordrecht.


Progress 06/01/10 to 05/31/11

Outputs
OUTPUTS: GPDN diagnosticians improved their capabilities with respect to rapid and accurate molecular diagnostic technologies and protocols having received advanced training through an array of formats from hands-on workshops to symposia sponsored by NPDN and by USDA-APHIS-PPQ-CPHST and Biotechnology laboratory. These training sessions focused on high consequence pathogens. Diagnosticians from the GPDN attended the Bioinformatics Workshop offered in August 2010, the P. ramorum training offered in April of 2010, and the Plum Pox Virus Workshop offered in April 2010. The training sessions were conducted by USDA-APHIS experts at the federal laboratory in Beltsville, Maryland. Diagnosticians from all nine GPDN states received some advanced training through an array of formats from hands-on workshops to symposia. GPDN diagnosticians participated in 1) the Thousand Cankers Workshop in CO. in June 2010, 2) a Tree Pest Workshop in Neb. in July 2010, and 3) an Adult Woodborer and Bark Beetle Workshop in Florida in May 2010. Once again this year, Montana State University (Linnea Skogland, Mary Burrows) hosted a GPDN webinar series from Jan. through April 2011. Nine speakers (regional and national) presented eight seminars on emerging diseases and related issues, including, Russian Wheat Aphid by Frank Peairs at CO. State, Nematodes of Corn by Tamra Jackson at the University of Neb.-Lincoln, Engaging Adult Learners by Shannon Arnold at Montana State, NPDN Foundation and Future by James Stack at KS State, Star-D by Karen Snover-Clift and Dawn O'Brien at Cornell, Bacteria in the Atmosphere by Gary Franc at the University of Wyoming, MgCl2 and Woody Plant Effects on Water Quality by Bill Jacobi at CO. State, and Bed Bugs by Janet Knodel at North Dakota State. These Adobe Connect webinars were very well attended with excellent question and answer sessions. GPDN labs entered the following number of samples into the NPDN National Data Repository through the PDIS database system: CO: 9479, KS: 985, MT: 3574, NE: 4584, ND: 5451, OK: 3515, SD: 1031, TX: 492, WY: 2212. The number of samples from GPDN member states received by the NPDN National Repository totaled 13,146 for the project year. All nine GPDN states participated in the final year for the GPDN Regional Wheat Virus Survey; 2010 was the third year of the survey. No new viruses were detected. The following viruses were detected: Wheat Streak Mosaic Virus, Barley Yellow Dwarf Mosaic Viruses - PAV & RPV, the virus that causes the High Plains Disease, Triticum mosaic virus, and Wheat Soilborne Mosaic Virus. First detector training was accomplished by GPDN diagnosticians and extension specialists through presentation and publication of material focusing on general plant pathology, diagnosing plant problems, GPDN and NPDN, pest emergencies, fungicide and pesticide resistance management, and specific plant disease and pest issues such as Tomato Leaf Curl Virus, wheat diseases, Head Scab in Wheat, sunflower diseases, and Pine Wilt Disease. Attendees included individuals from grower organizations, crop consultants, master gardeners, first detectors, horticultural society members and plant pathologists/ entomologists. PARTICIPANTS: GPDN member states (Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming) TARGET AUDIENCES: All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN National Data Repository for analysis by epidemiologists for the early detection of outbreaks. The activities of GPDN were described to partners and stakeholders in a variety of formats including formal reports and through participation in joint meetings. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The GPDN Regional Center provides guidance and management of the funding process to ensure that GPDN member states (Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming) completed necessary forms and adhered to guidelines and met all requirements. Guidelines were provided to ensure that the state plans of work addressed NPDN's primary program areas of 1) diagnostics, 2) training and education, and 3) communications and were consistent with the National Annual Plan of Work and the NPDN Strategic Plan. GPDN provided leadership for the PDIS diagnostic laboratory software system used by many states in the National Network. Products: All of the seminars presented in the GPDN webinar series are archived on the GPDN portal to facilitate asynchronous access to these valuable educational resources. Extension publications for first detectors were produced and disseminated. Dissemination: The mission and function of the diagnostic network was explained in presentations at national and international meetings. The activities of GPDN were described to partners and stakeholders in a variety of formats including formal reports and through participation in joint meetings. A change in knowledge: Diagnosticians -Diagnosticians throughout the Great Plains Region increased their knowledge of the diagnosis of high consequence plant pathogens and insect pests. This was accomplished through participation in lecture-based seminars delivered in person or over the web and by participation in hands-on laboratory workshops. Diagnosticians from GPDN states completed diagnostic training offered by USDA APHIS PPQ CPHST. The diagnosticians from these states are now prepared to conduct those protocols in their individual state diagnostic laboratories. Across the nine-state Great Pains Region, over 350 presentations were delivered to potential first detectors to prepare them to identify disease and pest outbreaks and to report them to appropriate authorities. A change in actions: All nine GPDN member state diagnostic laboratories implemented laboratory equipment calibration and maintenance plans to keep critical laboratory equipment in the running condition necessary to generate reliable and repeatable results. All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN National Data Repository for analysis by epidemiologists for the early detection of outbreaks.

Publications

  • No publications reported this period


Progress 06/01/09 to 05/31/10

Outputs
OUTPUTS: Diagnosticians from all nine GPDN states received some advanced training through an array of formats from hands-on workshops to symposia. GPDN members attended training programs sponsored by NPDN and by USDA-APHIS-PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory. These training sessions focused on molecular and morphological identification techniques for high consequence pathogens. Diagnosticians from the GPDN attended the Potato Wart Workshop offered in prior October and the Plum Pox Virus Workshop offered in April 2010. Four members (KS & TX) from the GPDN attended the 2.5 day Phytophthora kernoviae and P. ramorum training offered in prior March. Diagnosticians from all nine GPDN states received some advanced training through an array of formats from hands-on workshops to symposia. GPDN diagnosticians participated in 1) the Thousand Cankers Workshop in Colorado in October 2009 and again in March 2010, 2) a PCR Workshop in Kentucky in January 2010, and 3) an Africanized Honeybee Workshop in Kansas in April 2010. First Detector Training - GPDN members (diagnosticians and extension specialists) presented and published material focusing on general plant pathology, diagnosing plant problems, GPDN and NPDN, pest emergencies, fungicide and pesticide resistance management, and specific plant disease and pest issues such as wheat stem rust strain Ug99, Africanized Honey Bee, 1000 cankers disease, and Triticum mosaic virus. GPDN members conducted many training sessions for First Detectors. At least 174,534 First Detectors received some first detector training at 481 presentations. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak. Attendees included individuals from grower organizations, crop consultants, master gardeners, first detectors, horticultural society members and plant pathologists/ entomologists. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Data upload - All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN National Data Repository for analysis by epidemiologists for the early detection of outbreaks: CO: 2258, KS: 2017, MT: 1553, NE: 3721, ND: 824, OK: 3487, SD: 1977, TX: 796, WY: 376. The number of samples from GPDN member states received by the NPDN National Repository totaled 17,009 samples submitted. All nine GPDN states participated in the GPDN Regional Wheat Virus Survey; 2010 was the third year of the survey. No new viruses were detected. The following viruses were detected: Wheat Streak Mosaic Virus, Barley Yellow Dwarf Mosaic Viruses - PAV & RPV, the virus that causes the High Plains Disease, Triticum mosaic virus, and Wheat Soilborne Mosaic Virus. Management - Over $400,000 was distributed to GPDN member states and subcontractors to facilitate the purchase and maintenance of diagnostic equipment, training of first detectors, and the upload of diagnostic data to the NPDN National Data Repository. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The GPDN Regional Center provides guidance and management of the funding process to ensure that GPDN member states (Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming) completed necessary forms and adhered to guidelines and met all requirements. Guidelines were provided to ensure that the state plans of work addressed NPDN's primary program areas of 1) diagnostics, 2) training and education, and 3) communications and were consistent with the National Annual Plan of Work and the NPDN Strategic Plan. GPDN provided leadership for the PDIS diagnostic laboratory software system used by many states in the national Network. Products: All of the seminars presented in the GPDN webinar series are archived on the GPDN portal to facilitate asynchronous access to these valuable educational resources. Extension publications for first detectors were produced and disseminated. Dissemination: The mission and function of the diagnostic network was explained in presentations at national and international meetings. The activities of GPDN were described to partners and stakeholders in a variety of formats including formal reports and through participation in joint meetings. A change in knowledge: Diagnosticians - Diagnosticians through out the Great Plains Region increased their knowledge of the diagnosis of high consequence plant pathogens and insect pests. This was accomplished through participation in lecture-based seminars delivered in person or over the web and by participation in hands-on laboratory workshops. Diagnosticians from GPDN states completed diagnostic training offered by USDA APHIS PPQ CPHST. The diagnosticians from these states are now prepared to conduct those protocols in their individual state diagnostic laboratories. First detectors - Across the nine-state Great Pains Region, over 400 presentations were delivered to potential first detectors to prepare them to identify disease and pest outbreaks and to report them to appropriate authorities. A change in actions: All nine GPDN member state diagnostic laboratories implemented laboratory equipment calibration and maintenance plans to keep critical laboratory equipment in the running condition necessary to generate reliable and repeatable results. All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN National Data Repository for analysis by epidemiologists for the early detection of outbreaks.

Publications

  • Stack, J.P. 2010. Diagnostic networks for plant biosecurity. In, Knowledge and technology transfer for plant pathology. Hardwick, N. Gullino, M.L. eds. 123 p. Springer, Dordrecht.
  • Stack, J.P., Suffert, F., and Gullino, M.L. 2010. Bioterrorism: a threat to plant biosecurity In, The role of plant pathology in food safety and food security, Strange R.N., Gullino M.L. eds. 155 p. Springer, Dordrecht.


Progress 06/01/08 to 05/31/09

Outputs
OUTPUTS: Progress was made toward the goal for all GPDN labs to have molecular diagnostic capability. GPDN members attended training programs sponsored by NPDN and by USDA-APHIS. Training sessions focused on molecular and morphological identification techniques for high consequence pathogens. Two GPDN members attended the Potato Cyst Nematode training in 09/08 and 10/08. Four GPDN members attended the Phytophthora kernoviae and P. ramorum training in 03/09. Mary Burrows coordinated a three part workshop on the detection and identification of RNA viruses. Parts one and two (11/08 and 12/08) were offered on-line as Adobe Connect sessions. Experts in RNA virus isolation and detection provided the theory and protocols necessary for diagnosing plant diseases caused by RNA viruses. Part three of the series (01/09) was a two day hands-on workshop at NDSU hosted by Kasia Kinser. GPDN members presented and published material focusing on general plant pathology, diagnosing plant problems, GPDN and NPDN, pest emergencies, fungicide and pesticide resistance management, and specific plant disease and pest issues such as wheat stem rust strain Ug99, Africanized Honey Bee, 1000 cankers disease, and Triticum mosaic virus in 383 presentations/ publications that were attended/distributed by/to 23,176 participants. At least 5755 First Detectors received first detector training. Three GPDN Regional Center staff participated in a State of Kansas emergency response exercise conducted by the Kansas Dept. of Agriculture and USDA APHIS; two completed on-line incident command training. From 06/01/08 through 04/30/09, members entered 15,625 samples into the NPDN National Repository. GPDN Annual Meeting was held for member states; annual plans of work were developed, challenges were discussed, and solutions were derived. All 9 GPDN states participated in a regional wheat virus survey. The High Plains Disease in wheat and corn was detected in Montana and Wyoming for the first time. Triticum mosaic virus was detected in Colorado, Nebraska, Oklahoma, South Dakota, and Wyoming for the first time. A GPDN webinar series was conducted hosting eight speakers from the region and one from the Northeast Region on emerging diseases (1000 cankers disease of walnuts, Ug99), emerging insect pests (Russian Wheat Aphid), re-emerging diseases (Goss' Wilt of corn), and other programs (Master Gardener Training). GPDN Regional Center provided oversight to ensure that minimum expectations for funding were met including, participation at the annual regional meeting and in diagnostic training workshops, year end reporting, and involvement of both pathologists and entomologists in diagnostics and training programs. All seminars presented in the GPDN webinar series are archived on the GPDN portal to facilitate asynchronous access to these valuable educational resources. Extension publications for first detectors were produced and disseminated. The diagnostic network mission/function was explained in presentations at national and international meetings. GPDN activities were described to partners and stakeholders in a variety of formats including formal reports and through participation in joint meetings. PARTICIPANTS: Jim Stack, Ned Tisserat, Mary Burrows, Amy Ziems, Marcia McMullen, Rick Grantham, Larry Osborne, Ron French, Charlie Rush, Gary Franc TARGET AUDIENCES: Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, Wyoming PROJECT MODIFICATIONS: A new annual plan of work was developed in compliance with the NPDN Strategic Plan and Project Planning Matrix.

Impacts
A change in knowledge: Diagnosticians - Diagnosticians throughout the Great Plains Region increased their knowledge of the diagnosis of high consequence plant pathogens and insect pests. This was accomplished through participation in lecture-based seminars delivered in person or over the web and by participation in hands-on laboratory workshops. Two diagnosticians from Kansas and Montana completed the 2.5 day Potato Cyst Nematode diagnostic training offered by USDA APHIS PPQ CPHST in September and October 2008. Four diagnosticians from Kansas and Texas completed the 2.5 day Phytophthora kernoviae and P. ramorum diagnostic training offered in March of 2009. The diagnosticians from these states are now prepared to conduct those protocols in their individual state diagnostic laboratories. First detectors - Across the nine-state Great Pains Region, 385 presentations were delivered to potential first detectors to prepare them to identify disease and pest outbreaks and to report them to appropriate authorities. A change in actions: All nine GPDN member state diagnostic laboratories implemented laboratory equipment calibration and maintenance plans to keep critical laboratory equipment in the running condition necessary to generate reliable and repeatable results. All nine GPDN member state diagnostic laboratories uploaded diagnostic data to the NPDN National Data Repository for analysis by epidemiologists for the early detection of outbreaks.

Publications

  • Stack, J.P., 2008. Challenges to Crop Biosecurity. pp. 15-23. In: Crop biosecurity: assuring our global food supply. (Eds: Gullino M.L., Fletcher J., Gamliel A., Stack P.J.). Springer, The Netherlands. 148 pp.
  • Stack, J.P. and Baldwin, W. 2008. The need for secure communications networks and global connectivity. In: Crop biosecurity: assuring our global food supply. (Eds: Gullino M.L., Fletcher J., Gamliel A., Stack P.J.). Springer, The Netherlands. 148 pp.
  • Tinivella, F., Gullino, M.L., and Stack, J.P. 2008. The need for diagnostic tools and infrastructure. pp. 63-71. In: Crop biosecurity: assuring our global food supply. (Eds: Gullino M.L., Fletcher J., Gamliel A., Stack P.J.). Springer, The Netherlands. 148 pp.


Progress 06/01/07 to 05/31/08

Outputs
OUTPUTS: A comprehensive USDA CSREES five year program review document was completed and submitted to the review panel. The program review was completed, the review panel report evaluated, and all issues addressed in a response to the review panel. A GPDN regional meeting was convened in conjunction with the NPDN National Meeting. Progress was reviewed and plans for continued collaboration were developed. A national meeting of all member states and territories was convened in Orlando. It was attended by 226 representatives of the Network as well as Network partners including, government agencies, industry, and stakeholders. A GPDN regional training workshop on ornamental plants was convened for GPDN diagnosticians. A GPDN regional training workshop on nematode disease diagnosis and nematode identification was convened for GPDN diagnosticians. PARTICIPANTS: Jim Stack, Rick Grantham, Charlie Rush, Loren Geisler, Brad Gruden, Marica McMullen, Mary Burrows, Gary Franc, Ned Tisserat TARGET AUDIENCES: Kansas, Oklahoma, Texas, Nebraska, South Dakota, North Dakota, Montana, Wyoming, and Colorado PROJECT MODIFICATIONS: A revised strategic plan to guide the new Cooperative Agreement for NPDN was developed.

Impacts
A strategic plan meeting was convened to evaluate progress to date and to formulate a comprehensive plan for improving the NPDN and its regional networks. A region-wide collaborative wheat virus disease survey will be designed and implemented during the 2008 season. GPDN diagnosticians improved their skills in diagnosis of diseases and pathogens of ornamental plants and nematode caused diseases.

Publications

  • No publications reported this period