Progress 08/15/06 to 08/14/09
Outputs
OUTPUTS: A BAC library of the common bean containing an average of 12 genome equivalents was screened with mixtures of previously mapped random genomic clones (BNGs). BAC clones identified int he first screen were then isolated and used to produce dot-blots. These dot blots were hybridized individually with the radiolabeled mapped clones. Complementary to screening by hybridization, sequences of the previously mapped clones were used to search highly similar sequences derived from BAC-end sequences from the same BAC library. PARTICIPANTS: A post-doctoral fellow from Brazil, Dr. Francisco da Silva, was trained in C. Eduardo Vallejos' (PI) lab. The training consisted of handling a BAC library, isolation of BAC DNA, preparation of dot blots, and DNA hybridization with radiolabeled DNA probes. Ms Valerie Jones, a technician in the Vallejos's lab also made significant contributions to the project. TARGET AUDIENCES: The extent of synteny detected among legumes makes the legume research community the Target Audience for this project. The information generated by this project will likely benefit the community in regards to genome sequencing, gene mapping and breeding efforts as well. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Matching a BNG clone to a set of BAC clones automatically aligned the contig with which the BAC clones was associated with the linkage map of the common bean. Alignment of the physical and linkage maps will facilitate the completion of the physical map and future efforts to sequence the genome of the common bean, Phaseolus vulgaris.
Publications
- No publications reported this period
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