Progress 01/01/07 to 12/31/07
Outputs
The following techniques are used to study WNV: Classical virologic techniques and procedures including: Virus isolation and Serology, including serum neutralization, ELISA, western immunoblotting Molecular virologic and diagnostic techniques used include: Polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), nested PCR, real-time PCR, southern and northern blotting, nucleic acid sequencing, genome manipulation procedures such as infectious cDNA clones, modern strategies for nonreplicating vaccine development. Among the vaccines studied are: subunit vaccines, DNA vaccines, novel "targeted" vaccine delivery systems. Other services performed include production and characterization of monoclonal antibodies, epidemiology and molecular epidemiology, genetic characterization of microbial evolution, genetic determinants of virus persistence and virulence, pathology and pathogenesis, necropsy and histopathology, immunohistochemistry, in situ hybridization,
electron microscopy, flow cytometry and microarry
Impacts
This is a collaborative project which involves the Center for Equine Health and the Equine Viral Disease Research Laboratory. The goal is to maintain the diagnostic capability and expertise of the laboratory through strategic partnering with pre-eminent groups throughout the United States and the world. These collaborations have rapidly expanded the laboratory's capability so that the scientific staff have become facile in characterizing endemic diseases such as equine herpes, West Nile and equine viral arteritis, and diseases that currently are exotic to the United States such as African horse sickness.
Publications
- I. A. Gardner, S. J. Wong, G. L. Ferraro, U. B. Balasuriya, P. J. Hullinger, W. D. Wilson, P. Y. Shi, and N. J. MacLachlan. 2007, Incidence and effects of West Nile virus infection in vaccinated and unvaccinated horses. Vet. Res., 38:109-116. U. B. Balasuriya, P. Y. Shi, S. J. Wong, V. L. Demarest, I. A. Gardner, P. J. Hullinger, G. L. Ferraro, J. D. boone, C. L. DeCino, A. L. Glaser, R. W. Renshaw, M. Ledizet, R. A. Koski, and N. J. MacLachlan. 2006. Detection of antibodies to West Nile virus in eqine sera using microsphere immunoassay. J. Vet. Diagn. Invest., 18:392-395.
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Progress 04/01/05 to 11/30/05
Outputs
OUTPUTS: The following techniques are used to study WNV: Classical virologic techniques and procedures including: Virus isolation and Serology, including serum neutralization, ELISA, western immunoblotting Molecular virologic and diagnostic techniques used include: Polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), nested PCR, real-time PCR, southern and northern blotting, nucleic acid sequencing, genome manipulation procedures such as infectious cDNA clones, modern strategies for nonreplicating vaccine development. Among the vaccines studied are: subunit vaccines, DNA vaccines, novel "targeted" vaccine delivery systems. Other services performed include production and characterization of monoclonal antibodies, epidemiology and molecular epidemiology, genetic characterization of microbial evolution, genetic determinants of virus persistence and virulence, pathology and pathogenesis, necropsy and histopathology, immunohistochemistry, in situ hybridization, electron microscopy, flow cytometry and microarry PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
This is a collaborative project which involves the Center for Equine Health and the Equine Viral Disease Research Laboratory. The goal is to maintain the diagnostic capability and expertise of the laboratory through strategic partnering with pre-eminent groups throughout the United States and the world. These collaborations have rapidly expanded the laboratory's capability so that the scientific staff have become facile in characterizing endemic diseases such as equine herpes, West Nile and equine viral arteritis, and diseases that currently are exotic to the United States such as African horse sickness.
Publications
- No publications reported this period
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