Progress 05/01/06 to 06/01/08
Outputs
OUTPUTS: Growth factor enhanced progenitor cells for tendon healing has been submitted to the ACVS 2009 symposim. Stewart AA, Durgam S. PARTICIPANTS: Dr. Sushmitha Durgam assisted in this project during her Master's study. TARGET AUDIENCES: We are targeting the referring veterinarian's in an effort to help them understand the "stem cell" therapy and where we are really at scientifically. PROJECT MODIFICATIONS: The only change we have made in the study is to use a pulverized tendon matrix rather than a flat tendon matrix for the cells to adhere to. This allowed a more complete 3-dimensional matrix to form when compared to a monolayer type adherence to the surface of the tendon matrix. Our laboratory has used this model successfully with bone marrow-derived progenitor cells and a pulverized cartilage matrix.
Impacts
Both tendon and bone marrow-derived cells responded favorably to growth factor supplementation. In general tendon-derived cells were easier to culture and responded more consistently in cell culture to growth factor supplementation. IGF-I alone or in combination with FGF-2 had the most beneficial effects on tendon-derived cells adherence and viability, protein matrix synthesis, and mRNA expression. While bone marrow derived-cells showed similar effects, there was marked variation in the individual's response to growth factor supplementation. Most of the bone marrow-derived cells' lack of significant findings is due to the variability in response. The results of this study have supported further investigation using in-vivo models prior to use in a client owned animal. Although these results support the use of tendon-derived progenitor cells, the authors remain concerned about the benefits of using tendon-derived cells relative to the risks of collecting tendon-derived progenitor cells from the lateral digital extensor tendon. To date, eight lateral digital extensor tendons have been collected from live horses without any longterm complications.
Publications
- No publications reported this period
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Progress 10/01/06 to 09/30/07
Outputs
OUTPUTS: Durgam S, Stewart A, Stewart M, Byron C, Pondenis H. In vitro comparison of tendon and bone marrow-derived progenitor cells enhanced with IGF-1 cultured on a tendon matrix. Accepted for presentation at the Veterinary Orthopedic Society, March 2008, Big Sky, MT.
PARTICIPANTS: Sushmitha Durgam- BVSc, Graduate Student on the project. Allison Stewart- MS,DACVS,DVM, principle investigator on this project. Matthew Stewart- MS,PhD,BVSc,FACVS, co-investigator on this project. Christopher Byron- MS,DVM,DACVS, co-investigator on this project. Holly Pondenis- BS laboratory technician. This project was funded by the American Quarter Horse Foundation.
TARGET AUDIENCES: Our target audiences are equine veterinarians and horse owners. The project will be presented to enhance the knowledge of these audiences about the likely benefits of progenitor cells in tendon injuries.
PROJECT MODIFICATIONS: No major changes have been made.
Impacts
Tendon derived cells were easier to isolate, had a higher metabolic rate and propagate more readily in monolayer culture than bone marrow-derived stem cells. After 7 days of culture on tendon matrix, both tendon-derived and bone marrow-derived cells enhanced with IGF-I had an increase in cell adherence and viability when compared to the non IGF-I treated cells (P<0.001). A trend (p=0.052) in increased collagen synthesis was observed in the tendon derived progenitor cells treated with IGF-1. After 7 days of culture on matrix tendon- and bone marrow-derived progenitor cells showed no significant difference with reference to total DNA, total GAG content, and proteoglycans synthesis. These findings suggest we should further evaluate the possible use of tendon derived progenitor cells and IGF-1 in the treatment of tendinitis.
Publications
- No publications reported this period
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