Source: CORNELL UNIVERSITY submitted to NRP
SCREENING NATIVE POPULATIONS OF HEMLOCK FOR RESISTANCE TO HEMLOCK WOOLLY ADELGID
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0208986
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2006
Project End Date
Sep 30, 2009
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
ENTOMOLOGY
Non Technical Summary
Hemlock trees are being wiped out in the Eastern U.S. by hemlock woolly adelgid, an exotic pest. The character of natural areas and landscapes is being seriously altered because of the damage caused by the pest. Control of the pest is not practical in forested areas and in many environmentally sensitive landscapes. This project seeks to identify and propagate native trees that possess resistance to hemlock woolly adelgid. Such trees could be used to replant forests and landscapes that have been decimated by the adelgid.
Animal Health Component
65%
Research Effort Categories
Basic
35%
Applied
65%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2020610113040%
2022110113040%
2113110113020%
Goals / Objectives
1) Collect cones and cuttings of apparently healthy hemlocks growing in areas known to be infested with hemlock woolly adelgid. 2) Propagate hemlock seedlings from cones and cuttings. 3) Challenge seedlings with hemlock woolly adelgid to assess resistance status to the pest, and to preliminarily investigate resistance mechanism(s).
Project Methods
Cones and softwood cuttings of putatively resistant hemlocks will be collected from promising trees in Year 1 and Year 2. Trees serving as source material will be uninfested trees within areas known to infested with the adelgid. Cones will be collected after they mature (September-October) and dried in the laboratory under ambient conditions. Seed will be collected after cones open, and stored in the refrigerator in sealed containers. Portions of the seed from each of the parent trees will be subjected to the appropriate temperature treatments, following the protocols in Dirr & Heuser (1987), then planted in potting mix and grown in the greenhouse until they are of sufficient size to be assayed for susceptibility to the adelgid (ca. 3 in. tall). Softwood cuttings will be taken from parent trees in June and July. Cuttings will be 3-5 in. long, and will be kept cool and moist until they can be processed. No plant hormone treatments will be used when rooting the cuttings based on findings of Jetton et al. (2005), who found that softwood cuttings of T. canadensis rooted best when no NAA was used. Cuttings will be inserted into potting media in individual cells, and kept under a mist bench for 3-4 months, until rooting is evident. When seedlings are well established and have produced 2-3 in. of new shoot growth, they will be challenged in a quarantine greenhouse with hemlock woolly adelgid. Challenge will be accomplished by placing twigs of T. canadensis infested with eggs of hemlock woolly adelgid (collected during March from infested trees within the current infestation range) in contact with the test seedlings. Adelgid crawlers will move onto the test plants and produce the white, cottony masses typical of the adelgid if they are able to establish on the test plants. Seedlings with no signs of adelgid establishment will be selected for further propagation. Preliminary experiments to elucidate the mechanism of resistance will be conducted with cuttings from resistant and susceptible trees. Behavior of newly hatched adelgid crawlers will be observed on hemlock foliage under a stereomicroscope, and differential orientation of crawlers to needles of resistant and susceptible hemlocks will be assayed. In addition, mortality of adelgids confined separately with foliage of resistant and susceptible trees will be measured to determine if resistance results from toxicity. Other experiments will be conducted based on the results of these tests and other observations.

Progress 10/01/06 to 09/30/09

Outputs
OUTPUTS: Young hemlock trees were propagated from trees apparently resistant to hemlock woolly adelgid from three sites in the Hudson River Valley. Trees were rooted from cuttings in Year 1, and transferred to larger (4-inch) pots in Year 2. Because the Principal Investigator left the University in Year 2, the project was terminated at that point, and the resulting trees were transferred to researchers at the University of Rhode Island who had undertaken a project with virtually identical objectives. PARTICIPANTS: Cornell Cooperative Extension of Westchester County: Richard Harper and Jerry Giordano assisted with collection of tree cuttings. NYS Department of Environmental Conservation: Jerry Carlson and Lora Schwartzberg assisted with collection of tree cuttings. University of Rhode Island: Evan Preisser accepted transfer of cuttings to augment the collection of graduate student Laura Ingwell. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: The project was terminated in Year 2 because of the departure of the Principal Investigator, so Objective 3 could not be conducted.

Impacts
Rooting success of the cuttings taken in Year 1 decreased with collection date; rooting success declined from 42% for cuttings collected on March 1 to 17% for cuttings collected on March 23 (cuttings collected on March 13 had a rooting success of 22%). This is consistent with findings of researchers at the University of Rhode Island, who found that rooting success of cuttings collected in winter months was significantly higher than that of cuttings collected during the summer. It appears that environmental changes occurring in March are particularly critical to rooting success; other researchers attempting to conduct similar studies would be advised to take cuttings in January and February to maximize rooting success. Because the project was terminated early, unfortunately, we have no way to determine if the trees established from cuttings displayed resistance to hemlock woolly adelgid.

Publications

  • No publications reported this period


Progress 10/01/06 to 09/30/07

Outputs
OUTPUTS: During the first year of the project, we succeeded in establishing cuttings from 23 individual hemlock trees from areas infested by hemlock woolly adelgid. Cuttings were collected from 3 geographically distinct sites; from each site, 4-6 cuttings were collected from each of 13-15 trees. No cones were collected in Year 1 because virtually no cones were produced by trees in any of the 3 sites. Modest numbers of cones were produced in Year 2, and cones will be collected and processed according to plan. PARTICIPANTS: Richard Harper, Cornell Cooperative Extension of Westchester County Jerry Giordano, Cornell Cooperative Extension of Westchester County Jerry Carlson, NYS Department of Environmental Conservation Lora Schwartzberg, Pace University (undergraduate student) TARGET AUDIENCES: Nursery producers of hemlock trees, garden center operators, landscape managers, and homeowners.

Impacts
Rooting success decreased with time of collection of cuttings, ranging from over 60 percent for cuttings collected in late February to less than 20 percent for late March cuttings. The trees started from the cuttings are currently growing in a greenhouse, awaiting the time that they can be planted outdoors and challenged with adelgids (planned for Year 3 of the project). Establishment of plants is all that was expected for Year 1; the most interesting and useful information is expceted to accrue in Year 3 of the project, when trees can be assessed for their susceptbility/resistance to hemlock woolly adelgid.

Publications

  • No publications reported this period