Source: NORTH CAROLINA A&T STATE UNIV submitted to NRP
IMPROVING THE GREENHOUSE PRODUCTION PERFORMANCE OF CLERODENDRUM THOMSONIAE BALIF.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0208573
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2006
Project End Date
Sep 30, 2009
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
NORTH CAROLINA A&T STATE UNIV
1601 EAST MARKET STREET
GREENSBORO,NC 27411
Performing Department
NATURAL RESOURCES & ENV DESIGN
Non Technical Summary
The 2005 wholesale value of hanging baskets was $24.6 million in N.C. and $352.8 million in the U.S. Further expansion of this segment of floriculture depends on the development of new products (new crops or new ways to use/market old crops) Tropical bleeding-heart (Clerodendrum thomsoniae) is a plant that has been grown primarily in hanging baskets on a limited basis in the U.S. for many years. This plant has potential for further commercialization. Increased production depends on controlling the vigorous growth habit and increasing branching through cultivar selection, fertilization, and the application of chemical growth regulators. A series of five greenhouse experiments will be conducted with the overall goal to increase the commercialization of tropical bleeding-heart through improved production performance. Fertilization and plant growth regulator applications are two methods which will be investigated for achieving this goal. Commercial recommendations will then be prepared based on these results.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20421221060100%
Goals / Objectives
The objectives are to: 1) Determine the effects of N-P-K fertilization on the nutrient uptake, growth and development of C. thomsoniae. 2)Evaluate the effects of dikegulac-sodium, benzyl adenine (BA), and cyclanilide on branching of C. thomsoniae. 3) Determine the effects of substrate pH on the nutrient uptake, growth and development of C. thomsoniae. 4) Develop and record visual symptoms of nutrient deficiencies for C. thomsoniae. 5) Evaluate the effects of flurprimidol on plant growth and flowering of C. thomsoniae.
Project Methods
A series of five greenhouse experiments will be conducted with the overall goal to increase the commercialization of Clerodendrum thomsoniae (tropical bleeding-heart) through improved production performance. Mineral plant nutrition and plant growth regulator applications are two methods which will be investigated for achieving this goal. In the first experiment, investigate the effects of fertilizer rate on nutrient uptake, growth and development of C. thomsoniae with the intent of selecting an optimum rate. In the second experiment, several plant growth regulators (dikegulac-sodium, benzyl adenine (BA), and cyclanilide) known to promote branching will be evaluated on C. thomsoniae. In the third experiment, the effects of substrate pH on nutrient uptake, growth and development of C. thomsoniae will be observed. In the fourth experiment, C. thomsoniae will be grown in sand culture to produce eleven different mineral nutrient deficiencies (N, P, K, Ca, Mg, S, B, Fe, Mn, Zn, and Cu). Visual symptoms will be recorded and critical nutrient levels will be recorded using foliar analysis. In the fifth experiment, flurprimidol (a plant growth regulator which shortens internode length and increases flowering other species) will be tested C. thomsoniae. Data will be analyzed using the appropriate statistical analysis in SAS 8.2. Commercial recommendations will then be prepared based on these results. Results will be presented at scientific and grower meetings and in scientific journals and trade publications.

Progress 10/01/06 to 09/30/09

Outputs
OUTPUTS: Three plant growth regulator experiments were conducted on Clerodendrum thomsoniae. In Experiment 1, single applications of three plant growth regulators were applied as foliar sprays: dikegulac-sodium, [1000, 1333, 1667, 2000 mg /L (ppm)]; benzyl adenine [25, 50, 100, 200 mg/L (ppm)] and cyclanilide [25, 50, 100, 200 mg/L (ppm)] to determine efficacy on branching of C. thomsoniae. At the concentrations used, cyclanilide and BA had no effect on branching or shoot length. Dikegulac-sodium at 1667 mg/L initially increased branching by 300%, but after 16 weeks apical dominance was regained, which negated the initial increased branching response. Dikegulac-sodium also delayed flowering. In Experiments 2 and 3, single drench applications of flurprimidol were applied in two different concentration ranges [(0.25, 0.5, 1, 2, 4 mg a.i./pot) and ( 0.0675, 0.125, 0.25, 0.5, 1 mg a.i./ pot), respectively] to determine the efficacy on growth inhibition. A quadratic response to flurprimidol was observed in both experiments. Ancymidol was tested at 0.5 and 1 mg/L in the first experiment with similar results to flurprimidol. Two mineral nutrition experiments, a fertilizer concentration study and nutrient disorders study, were also conducted. In Experiment 4, a green-leaf selection was grown in 2.6 L azalea pots for 129 d under five constant liquid fertilizer concentrations (50, 100, 200, 300, and 400 mg/L N). Plants were destructively harvested at 37, 69, 97, and 129 days after planting and analyzed for nutrient content. Linear responses to the fertilizer levels were observed for shoot length, dry weight, pH, and EC at 69 and 129 d. In Experiment 5, elemental deficiencies of N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, and B and toxicity of B were induced in a variegated selection. Rooted stem cuttings were planted in 1.29- L pots containing silica sand and fertilizer with a modified complete Hoagland's solution or this solution minus the element investigated. A solution containing excess B was also included in the study. Plants were harvested for nutrient analysis when initial symptoms were expressed. Nutrient deficiency symptoms were described. Foliar tissue concentration data will assist plant tissue analysis laboratories in establishing foliar nutrient standards for growers. PARTICIPANTS: Drs. M. Reddy, C. Niedziela, B. Whipker and Karen Neal a graduate student (M.S.), and one undergraduate student worked on the project. Dr. Whipker is from North Carolina State University TARGET AUDIENCES: Floriculture Industry, Nurseries and Green Industry. PROJECT MODIFICATIONS: Cyclanilide and benzyl adenine had no effect on branching of shoot length at the concentrations used on Clerodendrum thomsoniae. Dikegulac-sodium also delayed flowering. For Clerodendrum thomsoniae 200 mg/L nitrogen is recommended because it provided adequate fertility without excessive growth. Nutrient deficiency symptoms were described and foliar nutrient concentrations provided.

Impacts
The vine Clerodendrum thomsoniae has the potential to further expand the green industry by providing a new flowering ornamental useful for hanging baskets.

Publications

  • No publications reported this period


Progress 01/01/08 to 12/31/08

Outputs
OUTPUTS: Three plant growth regulator experiments were conducted on Clerodendrum thomsoniae. In Experiment 1, single applications of three plant growth regulators were applied as foliar sprays: dikegulac-sodium, [1000, 1333, 1667, 2000 mg /L (ppm)]; benzyl adenine [25, 50, 100, 200 mg/L (ppm)] and cyclanilide [25, 50, 100, 200 mg/L (ppm)] to determine efficacy on branching of C. thomsoniae. At the concentrations used, cyclanilide and BA had no effect on branching or shoot length. Dikegulac-sodium at 1667 mg/L initially increased branching by 300%, but after 16 weeks apical dominance was regained, which negated the initial increased branching response. Dikegulac-sodium also delayed flowering. In Experiments 2 and 3, single drench applications of flurprimidol were applied in two different concentration ranges [(0.25, 0.5, 1, 2, 4 mg a.i./pot) and ( 0.0675, 0.125, 0.25, 0.5, 1 mg a.i./ pot), respectively] to determine the efficacy on growth inhibition. A quadratic response to flurprimidol was observed in both experiments. Ancymidol was tested at 0.5 and 1 mg/L in the first experiment with similar results to flurprimidol. Two mineral nutrition experiments, a fertilizer concentration study and nutrient disorders study, were also conducted. In Experiment 4, a green-leaf selection was grown in 2.6 L azalea pots for 129 d under five constant liquid fertilizer concentrations (50, 100, 200, 300, and 400 mg/L N). Plants were destructively harvested at 37, 69, 97, and 129 days after planting and analyzed for nutrient content. Linear responses to the fertilizer levels were observed for shoot length, dry weight, pH, and EC at 69 and 129 d. In Experiment 5, elemental deficiencies of N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, and B and toxicity of B were induced in a variegated selection. Rooted stem cuttings were planted in 1.29- L pots containing silica sand and fertilizer with a modified complete Hoagland's solution or this solution minus the element investigated. A solution containing excess B was also included in the study. Plants were harvested for nutrient analysis when initial symptoms were expressed. Nutrient deficiency symptoms were described. Foliar tissue concentration data will assist plant tissue analysis laboratories in establishing foliar nutrient standards for growers. PARTICIPANTS: Drs. M. Reddy, C. Niedziela, B. Whipker and Karen Neal a graduate student (M.S.), and one undergraduate student worked on the project. Dr. Whipker is from North Carolina State University TARGET AUDIENCES: Audience includes -- Floriculture Industry, Nurseries and Green Industry. PROJECT MODIFICATIONS: Cyclanilide and benzyl adenine had no effect on branching of shoot length at the concentrations used on Clerodendrum thomsoniae. Dikegulac-sodium also delayed flowering. For Clerodendrum thomsoniae 200 mg/L nitrogen is recommended because it provided adequate fertility without excessive growth. Nutrient deficiency symptoms were described and foliar nutrient concentrations provided.

Impacts
The vine Clerodendrum thomsoniae has the potential to further expand the green industry by providing a new flowering ornamental useful for hanging baskets.

Publications

  • Karen I. Davis, Carl E. Niedziela Jr., Muchha R. Reddy, Brian E. Whipker, and Jonathan M. Frantz. 2009. Nutrient Requirements and Disorder Symptomology of Clerodendrum thomsoniae. Journal of Plant Nutrition (Manuscript: in review).
  • Davis, Karen I., 2008. Effects of plant growth regulators and mineral plant nutrition on Clerodendrum thomsoniae (Thesis: M.S.).


Progress 01/01/07 to 12/31/07

Outputs
OUTPUTS: Experiment 1. To determine foliar symptomology of nutrient deficient Clerodendrum thomsoniae a greenhouse study was conducted. Rooted cuttings of Clerodendrum thomsoniae were planted in 5.25-inch pots containing 1.37 quart of acid washed silica-sand on May 4, 2007. The temperature in the greenhouse was set at day/ night temperatures of 24/18 centigrade. Plants were fertigated every two hours an automated irrigation system with a modified Hoagland nutrient solution or absent one of the eleven essential mineral nutrients. The experimental design was a randomized complete block with three replications. Deficiency of nitrogen resulted in pale green leaves from day 27, the plants were stunted, older leaves chlorotic and no visible flowers whereas the control treatment was in flower. Phosphorus deficiency caused slightly chlorotic leaves and leaf margins rolled up. By day 63, yellowing, defoliation and curling symptoms became severe. Potassium deficiency developed chlorotic older leaves with necrotic edges. Calcium deficiency caused aborted flowers at 55 days. Magnesium deficiencies resulted in intervienal chlorosis of the leaves. Micronutrient deficiencies were observed from 42 to 63 days. Born deficiencies resulted in aborted flowers, thickened and curled leaves. Experiment 2. Effect of three plant growth regulators on branching of Clerodendrum thomsoniae was evaluated in the greenhouse. Single applications of: Dikegulac-sodium, 1000, 1333, 1667, 2000 mg/liter; benzyl adenine 25, 50, 100, 200 mg/liter and Cyclanilide 25, 50, 100, 200 mg/liter to determine its efficacy on branching of Clerondrum thomsoniae. An untreated control was also maintained. Cyclanilide and Benzyl-adenine had no effect on branching and shoot length in Clerondendrum. Dikegulac-sodium increased branching initially, but the response decreased over time. Dikegulac- sodium also delayed flowering. PARTICIPANTS: The project was conducted by Drs. Niedziela, Whipkerand Reddy and Karen Neal a graduate student(M.S.). Dr. Whipker is from North Carolina State University, other participants are from N.C A&T State University. TARGET AUDIENCES: Green Industry,Nurseries and Floriculture Industry

Impacts
The green industry is North Carolina's third largest agricultural industry. This project has the potential to further expand this industry by providing a new flowering ornamental useful for hanging baskets.

Publications

  • No publications reported this period


Progress 01/01/06 to 12/31/06

Outputs
The objectives of this study are to: 1. Determine the effects of N-P-K fertilization on the nutrient uptake, growth and development of C. thomsoniae. 2. Evaluate the effects of dikegulac-sodium, benzyl adenine (BA), and cyclanilide on branching of C. thomsoniae. 3. Determine the effects of substrate pH on the nutrient uptake, growth and development of C. thomsoniae. 4. Develop and record visual symptoms of nutrient deficiencies for C. thomsoniae. 5. Evaluate the effects of flurprimidol on plant growth and flowering of C. thomsoniae. A series of five greenhouse experiments will be conducted over next three years with the overall goal to increase the commercialization of Clerodendrum thomsoniae (tropical bleeding-heart) through improved production performance. Mineral plant nutrition and plant growth regulator applications are two methods which will be investigated for achieving this goal. Commercial recommendations will then be prepared based on these results. Results will be presented at scientific and grower meetings and in scientific journals and trade publications.

Impacts
NA

Publications

  • No publications reported this period