Source: AGRICULTURAL RESEARCH SERVICE submitted to NRP
CHARACTERIZATION AND CHEMISTRY OF SEXUAL COMMUNICATION IN PEAR PSYLLA, CACOPSYLLA PYRICOLA (HOMOPTERA: PSYLLIDAE)
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0207904
Grant No.
2006-35302-17475
Cumulative Award Amt.
$233,000.00
Proposal No.
2006-01793
Multistate No.
(N/A)
Project Start Date
Sep 1, 2006
Project End Date
Aug 31, 2009
Grant Year
2006
Program Code
[51.2]- (N/A)
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
5230 KONNOWAC PASS ROAD
WAPATO,WA 98951
Performing Department
(N/A)
Non Technical Summary
Pear psylla is the main pest of pear in the United States. Growers need lures and traps to monitor pear psylla in commercial pear orchards. This project seeks to develop a sex pheromone produced by female pear psylla to attract males. The purpose of this study is to identify the pheromone and determine how it functions to attract pear psylla.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21111151130100%
Goals / Objectives
To isolate and identify sex pheromone of the pear psylla and understand mating and host finding behavior. Ultimate project objective is to provide pear growers with monitoring techniques for the pear psylla, a key pest of pear.
Project Methods
We will use a suite of behavioral assays, electroantennography, volatile collections, and GC-MS techniques to determine physiological and environmental parameters controlling psylla reproductive behavior, develop assays for chemical attractants, characterize the odor profile of psylla and pear foliage, and isolate and identify pheromone and possibly host kairomone compounds. Identified chemical attractants may need to be synthesized and purified, if not commercially available. Subsequent work will then focus on controlled release dispensers or lures, and use of the attractant in traps.

Progress 09/01/08 to 08/31/09

Outputs
OUTPUTS: Attractant volatiles from female pear psylla were identified and synthesized. Laboratory and field tests confirmed that the synthesized product was attractive to male pear psylla. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The research has led to the first identification of an attractant sex pheromone in any member of the Psyllidae.

Publications

  • Guedot, C., D.R. Horton and P.J. Landolt. 2009. Attraction of male winterform pear psylla to female-produced volatiles and to female extracts and evidence of male-male repellency. Entomologia Experimentalis et Applicata 130: 191-197.
  • Guedot, C., J.G. Millar, D.R. Horton, and P.J. Landolt. 2009. Identification of a sex attractant pheromone for male winterform pear psylla, Cacopsylla pyricola. Journal of Chemical Ecology 35: 1437-1447.


Progress 09/01/07 to 08/31/08

Outputs
OUTPUTS: Volatiles were collected and assayed for attractiveness to male pear psylla. The volatiles were collected as surfaces washes of insects and as headspace collections. Males were attracted to the crude extracts in olfactometer assays. A comparison of chromatograms from female- and male-washes led to identification of chemicals present in female but not male washes. Those chemicals were assayed in the olfactometer and found to be attractive to males. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Assays have led to the discovery of specific chemicals that are attractive to male pear psylla, and may lead eventually to the first identification of a sex pheromone in any psyllid.

Publications

  • Horton, D.R., C. Guedot and P.J. Landolt. 2008. Attraction of male summerform pear psylla to volatiles from female pear psylla: effects of female age, mating status, and presence of host plant. Canadian Entomologist 140: 184-191.


Progress 09/01/06 to 08/31/07

Outputs
Life history characteristics of female and male pear psylla that affect female attractiveness to males were identified using a series of olfactometer assays. Diapause status and age affected female attractiveness. Mating status of females did not affect attractiveness. Males were attracted to females even in the absence of the host plant. Field-collected females, freshly killed, were also attractive to males, again demonstrating that the host plant need not be present to have production of chemical volatiles that attract males. Efforts are underway to collect volatiles from females, with objectives to identify the attractants.

Impacts
Behavioral assays will define the life history characteristics in female pear psylla that lead to attraction of males. Volatiles will next be collected from females having those specific characteristics that lead to male attraction, for eventual identification of the chemicals that mediate male attraction.

Publications

  • Horton, D.R., and Landolt, P.J. 2007. Attraction of male pear psylla, Cacopsylla pyricola, to female-infested pear shoots. Entomol. Exp. Appl. 123: 177-183.
  • Horton, D.R., Guedot, C. and Landolt, P.J. 2007. Diapause status of females affects attraction of male pear psylla, Cacopsylla pyricola, to volatiles from female-infested pear shoots. Entomol. Exp. Appl. 123: 185-192.