Progress 09/01/06 to 08/31/09
Outputs
OUTPUTS: A Seed Technology Laboratory has been developed at SDSU for the purpose of developing, implementing, and coordinating research, laboratory analysis/testing, academic, and extension/outreach programs in the areas of seed science and technology, and seed biotechnology. Two additional areas of focus were merged in with the overall project and the mission has shifted to include drought stress with a seed focus, and a crop quality laboratory for both research and evaluation of materials in the plant breeding programs at SDSU. A new $6.5 million facility funded by commodity groups and other private sources to house the program is almost finished and will be occupied in 2010. This facility will bring together the faculty and allow an enhanced focus and collaboration that will foster new advances. Drs. Gu and Gonzalez have been absorbed into more of the drought stress biotechnology and genomics side with a seed focus and their research is geared towards multiple collaborations with research faculty. Dr. Gonzalez works with a team of wheat breeders, a pathologist, and others to utilize molecular tools to identify genes linked to biotic stresses of disease and pest resistance. He is also becoming well-recognized for his work on prairie cordgrass (Spartina pectinata) genomics. Prairie cordgrass is a potentially valuable bio-mass crop in the cellulosic ethanol production field. Dr. Gonzalez is part of team of scientists collaborating on prairie cordgrass research. Dr. Gu has built a renowned seed molecular program that focuses on identifying genes in rice that control seed dormancy, with the end goal of using that information in developing sprout resistant wheat varieties through biotechnology. He has collaborations with other public university research faculty in ND and MN. Dr. Gu also works with the soybean entomologist on biotic/abiotic stress resistance, and has also looked at field research to identify soybean germplasm resistant to iron chlorosis. Both Drs. Gu and Gonzalez have published numerous referred publications on their research. Dr. Turnipseed has continued to expand seed testing capabilities in the SDSU Seed Testing Laboratory and provides direction, space, material support and training for multiple graduate students, faculty, and post-docs in seed germination research in addition to service testing. PARTICIPANTS: Brent Turnipseed, Professor/Manager, SDSU Seed Testing Laboratory, was the PI for this project. His role was to pull together a team in the area of Seed Technology. His specific research focus area was seed testing, seed dormancy and vigor. Neal Foster, Executive Director, South Dakota Crop Improvement Association, was the CoPI and his role was to help secure funding for a new facility, provide funding for research, and shepherd along the facility construction. Xingyou Gu, Associate Professor, was hired as a seed biotechnologist/physiologist specializing in genomics and seed molecular biology at SDSU. Jose Gonzalez, Assistant Professor, was hired as a seed biotechnologist specializing in genomics and plant molecular genetics at SDSU. Partner organizations would include South Dakota State University, and the South Dakota Crop Improvement Association. TARGET AUDIENCES: Target audiences would be university and private seed researchers, seed testing laboratories, rice breeders, wheat breeders, soybean breeders, genomic researchers, cellulosic ethanol producers, biomass researchers, seed companies, and crop Improvement associations. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
The Seed Technology project is a long-term project that will continue to evolve and develop. A new Seed Technology Laboratory facility is nearing completion and has been paid for through primarily private sector (commodity groups and other private donors) funding and some collaborative funding from SD Drought Stress Center funding (towards the greenhouse complex). The facility includes multiple modern molecular research laboratories, a crop quality lab for milling and baking characteristics of grain, a seed testing service and research lab, 6 faculty, numerous graduate students, research technicians and the South Dakota Crop Improvement headquarters (a major building donor). Dr. Gu's work is a long-term research project and to date he has identified specific genes in weedy rice that are implicated with seed dormancy control. This is work that someday will help in breeding white wheat lines for the region that have sprout resistance (or dormancy genes). Dr. Gonzalez's work with the wheat breeders and pathologist will result in identifying genes that control disease resistance and other desired quality traits. His ground-breaking collaborative research on the prairie cordgrass genome will help with the breeding and selection of genetic material best suited for biomass production. He continues to work with a group of collaborators on the development of prairie cordgrass as a biomass crop. Dr. Turnipseed's graduate student research work on germination of prairie cordgrass has led to the identification of optimal germination conditions in a seed testing lab for this species. The work can now be continued with referee testing to propose a new rule proposal in the Association of Official Seed Analyst Rules for Testing Seed. Drs. Gu, Gonzalez and Turnipseed have 3 PhD candidate students, 4 MS candidate students, 2 postdocs, 4 research technicians, 1 support staff, and 12 undergraduate students working in their research labs. With the Seed Technology facility completed, SDSU looks forward to future research and development collaborations with the private sector in addition to other public research faculty.
Publications
- Gu, X.-Y., T. Liu, J. Feng, J.C. Suttle, J. Gibbons. 2009. The qSD12 underlying gene promotes abscisic acid accumulation in early developing seeds to induce primary dormancy in rice. Plant Molecular Biology (In press, DOI: 10.1007/s11103-00995551). Epub 2009 Oct 13.
- Gonzalez-Hernandez, J., Sarath, G., Owens, V., Stein, J.M., Gedye, K. 2009. A Multiple Species Approach to Biomass Production from Native Herbaceous Perennial Feedstocks. In Vitro Cellular and Developmental Biology - Plants. 45:267-281.
- Gonzalez-Hernandez, J.L., Singh, P.K., Mergoum, M., Adhikari, T.B., Kianian, S.F., and Elias, E.M. 2009. A quantitative trait locus on chromosome 5B controls resistance of Triticum turgidum (L.)var. diccocoides to Stagonospora nodorum blotch. Euphytica. 166 (2): 199. K.E. Lamb, R.D. Horsley, B. Zhang, J.L. Gonzalez-Hernandez, M. Green, and P.B. Schwarz. 2009. Inheritance and QTL Associated with Fusarium Head Blight Resistance in the Breeding Line C93-3230-24. Crop Science. 49(5):1675-1680.
- Boe, A., Owens, V., Gonzalez-Hernandez, J., Stein, J., Lee, D., and Koo, B.-C. 2009. Morphology and Biomass Production of Prairie Cordgrass on Marginal Lands. Global Change Biology Bioenergy. 1:240-250.
- Rosyara, U.R., Gonzalez-Hernandez, J.L., Glover, K.R., Gedye, K.R. and Stein, J.M. 2009. Family-based mapping of quantitative trait loci in plant breeding populations with Fusarium head blight resistance in wheat as an illustration. Theoretical and Applied Genetics. 118:1617-1631. Xu, L., B.E., Turnipseed, R.N. Gates, P. S. Johnson, and N.H. Jr. Troelstrup. 2009. Range management and soil seed bank: new perspective. Proc. of the South Dakota Academy of Science. Vol. 83:183
- Xu, L., B.E., Turnipseed, R.N. Gates, P. S. Johnson, and N.H. Jr. Troelstrup. 2009. Effects of different management practices on soil seed bank composition in a mixed-grass prairie of the northern Great Plains. Soc. Range Manage Vol. 62: No. 1030-16
- Korman, B.L., G.E. Larson, J.L. Butler, and E.B. Turnipseed. 2009. Abstract. Germination Characteristics of Sickleweed (Falcaria vulgaris) from the Fort Pierre National Grassland of South Dakota. Proceedings of the South Dakota Academy of Science. Vol. 88: 181.
- Schanning, Greta L. 2009. Identifying the Optimal Laboratory Germination Testing Regime Needed for Prairie Cordgrass (Spartina pectinata L.). A research paper presented to the Plant Science Department as a partial requirement for MS Option B graduation. (Non-refereed).
|
Progress 09/01/07 to 08/31/08
Outputs
OUTPUTS: A Seed Technology Laboratory has been created at South Dakota State University for the purpose of developing, implementing, and coordinating research, laboratory analysis/testing, academic, and extension/outreach programs in the areas of seed science, technology, and biotechnology. Two additional focus areas have been merged in with the overall project and goals, one in drought stress biotechnology research, and the other in crop/grain quality research and evaluation. Research and grants that have been obtained and are ongoing are listed below. Many are multi-year, and most have multiple collaborators not listed. Drs. Gu, Gonzalez, and Turnipseed have 1 post-doc, 9 graduate students (MS and PhD level), 1 visiting scientist, 7 full-time staff, and 23 undergraduate students working in their labs. Additional funds for research programs and facilities continue to be sought through partnerships with crop commodity groups and private industry, including seed companies and seed testing labs, as well as through other grant opportunities. Coordination with other departments, universities and private industry will help ensure relevance of activities and programs. PARTICIPANTS: Dr. Xingyou Gu, Assistant Professor, South Dakota State University Dr. Jose L. Gonzalez, Assistant Professor, South Dakota State University Dr. Brent Turnipseed, Professor, South Dakota State University Dr. Neal Foster, Executive Director, SD Crop Improvement Association Current Partner Organizations - South Dakota Soybean Council, South Dakota Wheat Commission, South Dakota Corn Council, SD Oilseeds Council, South Dakota Crop Improvement Association, and South Dakota Foundation Seed. TARGET AUDIENCES: Other seed researchers, seed companies, breeders, future collaborators, and seed testing laboratories are the primary target audience. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
The SDSU Seed Technology program synergize with the Drought Stress and Crop Quality labs in a new facility, and will collaboratively conduct both applied and fundamental research into seed genetics, seed technology and biotechnology. These interactions will continue to expand and will help improve seed and crop quality characteristics and traits for the agricultural industry. The program will enhance and increase opportunities for research and training of students who will be tomorrow's leaders in the agricultural industry. Over six and a quarter million dollars from commodity groups and private companies have been committed for construction of a new facility to address these issues and more. The SDSU Seed Technology/Drought/Crop Quality programs will provide and promote unbiased research, laboratory testing, teaching, and extension/outreach programs.
Publications
- J.L. Gonzalez-Hernandez, P.K. Singh, M. Mergoum, T.B. Adhikari, S.F. Kianian, & E.M. Elias.. 2008. A quantitative trait locus on chromosome 5B controls resistance of Triticum turgidum (L.) var. diccocoides to Stagonospora nodorum blotch. Submitted to Euphytica.
- U.R. Rosyara, K.L. Maxon-Stein, K. Gedye, K.D. Glover, J.M. Stein, and J.L. Gonzalez-Hernandez. 2008. Family-based mapping of quantitative trait loci in plant breeding populations with an illustration of head scab resistance in wheat . To be Submitted to Theoretical and Applied Genetics.
- K. D. Gedye, KL. Maxon-Stein, C. Shaefer, V. Owens, A. Boe and J.L. Gonzalez-Hernandez. 2008. Characterization of microsatellite markers in Prairie cordgrass (Spartina pectinata). Manuscript in preparation to be submitted to Molecular Ecology Notes.
- Kenneth E. Lamb, Richard D. Horsley, Bingxin Zhang, Jose L., Gonzalez-Hernandez , Marci Green, and Paul B. Schwarz . 2008. Inheritance and QTL Associated with Fusarium Head Blight resistance in the Breeding Line C93-3230-24 Manuscript in preparation to be submitted to Crop Science.
- Gu, X.-Y., M. E. Foley, J. V. Anderson, and D. P. Horvath. 2008. qSD7-1 is the first dormancy QTL cloned from weedy rice. Polish J. Natural Sci. suppl. 5:51.
- Cho, Y., E.B. Turnipseed, D.A. Lightfoot and A.J. Wood. 2008. Trigonelline in mature seeds and developing seedlings of Glycine max Biologia Plantarum 52 (2): 370-372
- Hall, A. and B. Turnipseed. 2008. 2008 AOSA/SCST Annual meeting: An Ovation to Mutual Purpose. Seed Today Vol. 12(2). p. 26
- Hall, A. and B. Turnipseed. 2008. 2008 Proposed Amendments to AOSA Rules for Testing Seeds. Seed Today Vol. 12(1). p. 30
- Hall, A. and B. Turnipseed. 2007. Testing for the Presence of RR Alfalfa Seed. Seed Today Vol. 11(3). p. 24
- Hall, A. and B. Turnipseed. 2007. How End Use Affects Laboratory Seed Testing. Seed Today Vol. 11(4). p. 18
|
Progress 09/01/06 to 08/31/07
Outputs
A Seed Technology Laboratory has been created at South Dakota State University for the purpose of developing, implementing, and coordinating research, laboratory analysis/testing, academic, and extension/outreach programs in the areas of seed science, technology, and biotechnology. Two Assistant Professors were hired in 2006 to improve and expand research capabilities. Two additional focus areas will be merged in with the overall project and goals, one in drought stress biotechnology research, and the other in crop/grain quality research and evaluation. Research and grants that have been obtained and are ongoing are listed below. Many are multi-year, and most have multiple collaborators not listed. Molecular Characterization of Spring and Winter Wheat Germplasm. Protein Quality. PI: Jose L. Gonzalez. SD Wheat Commission grant. Metabolomics and Functional Genomics of Seed Lipid Biosynthesis in Cuphea. PI: Jose L. Gonzalez Identifying Seed Dormancy Genes from Wild
Germplasm to Improve Resistance of Common Wheat Varieties to Pre-Harvest Sprouting By Marker-Assisted Selection. PI: Xingyou Gu. SD Wheat Commission Grant. Toward Map-Based Cloning of Seed Dormancy QTLs in Rice. PI: Xingyou Gu. SDSU Agricultural Experiment Station In-House grant. Equipment Grants. PI: Xingyou Gu. SDCIA and FSSD grants. Toward Map-Based Cloning of Seed Dormancy QTLs in Rice. PI: Xingyou Gu. Characterization of the Major Dormancy gene at qSD12 in Rice. PI: Xingyou Gu. NSF grant. Development of rice as an alternative crop for South Dakota. Co-PI: Xingyou Gu. SDCIA grant. Environmental Factors Influencing FHB and DON Accumulation in Hard Red Wheat. Co-PI: Jose L. Gonzalez. USDA-ARS USWBSI grant. Molecular Breeding of Spring and Winter Wheat Germplasm for end use quality and disease resistance. PI: Jose L. Gonzalez. SD Wheat Commission grant. Developing a Sustainable Feedstock and Next-Generation Processing Technologies for Biofuels Production. Co-PI: Jose L. Gonzalez. NC
SunGrant grant. Developing a Prairie Biorefinery. Co-PI: Jose L. Gonzalez. Introgression of a new glyphosate resistance gene into SD lines. SD Soybean Council grant. PI: Jose L. Gonzalez. Germination and Storage Studies on Prairie Cordgrass. PI: Greta Scanning and Brent Turnipseed. Topic of Graduate Research Assistantship. Equipment Grants through various entities (SDWC, SDCIA, FSSD, AES, PS, USDA). CoPI/Collaborator: Brent Turnipseed. Total Grant funding received for above projects exceeds $3.4 million. Drs. Gu and Gonzalez have 2 post-docs, 3 PhD students, 1 MS student, 1 visiting scientist, two technicians, and 7 undergraduate students working in their research labs. Dr. Turnipseed has 1 MS student, 5 permanent and several students working in the seed testing lab. Additional funds for research programs and facilities continue to be sought through partnerships with crop commodity groups and private industry, including seed companies and seed testing labs, as well as through other
grant opportunities. Coordination with other universities and private industry will help ensure relevance of activities and programs.
Impacts
The SDSU Seed Technology program will collaboratively conduct both applied and fundamental research into seed genetics, seed technology and biotechnology, which will help improve seed and crop quality characteristics and traits for the agricultural industry. The program will enhance and increase opportunities for research and training of students who will be tomorrow's leaders in the agricultural industry. Over four and a half million dollars from commodity groups and private companies have been committed for construction of a new facility to address these issues and more. The SDSU Seed Technology program will provide and promote unbiased research, laboratory testing, teaching, and extension/outreach programs.
Publications
- Foley, M.E., X.-Y. Gu, S.F. Kianian. 2007. QTLs, epistasis, and other interactions associated with dormancy in weedy rice (Oryza sativa L.). In: Adkins S., S. Ashmore S. and S. C. Navie (eds), Seeds: Biology, Development and Ecology. CABI Publishing.
- J.L. Gonzalez-Hernandez, Glover, K., Stein, J. and Chen, D. 2006. A Novel Approach towards Molecular Characterization and Pyramiding of Novel Scab Resistance Sources. In: Canty, S.M., Lewis, J., Siler, L. and Ward, R.W (Eds.), Proceedings of the National Fusarium Head Blight Forum; 2006 Dec 10-12; Research Triangle Park, NC. East Lansing: Michigan State University. pp. 22-25.
- Gu, X.-Y., M.E. Foley. 2007. Epistatic interactions of three loci regulate flowering time under short and long daylengths in a backcross population of rice. Theor. Appl. Genet. 114: 745-754.
- Foley, M.E., X.-Y. Gu, S.F. Kianian. 2007. QTLs, epistasis, and other interactions associated with dormancy in weedy rice (Oryza sativa L.). In: Adkins S., S. Ashmore S. and S. C. Navie (eds), Seeds: Biology, Development and Ecology. CABI Publishing.
- Accepted but not yet published: Singh, P. K., M. Mergoum, J. L. Gonzalez-Hernandez, S. Ali, T. B. Adhikari, S. F. Kianian, E. M. Elias, and G. R. Hughes. 2008. Genetics and Molecular Mapping of Resistance to Necrosis Inducing Race 5 of Pyrenophora tritici-repentis in Tetraploid Wheat. Accepted in Molecular Breeding
- Pending: Gu, X.-Y., E. B. Turnipseed, and M. E. Foley. 2007. The qSD12 Locus Controls Offspring Tissue-Imposed Seed Dormancy in Rice. Genetics.
|
|