Progress 08/01/06 to 07/31/07
Outputs
OUTPUTS: Outputs: Activities. Directly related to the proposed work, I developed assay conditions to assess photoperiodic regulation of sexual reproduction in Physcomitrella patens. I constructed double PpCOL knockout lines via transformation and PCR confirmation of gene replacement, and carried out initial phenotypic analysis of these lines. I also obtained a series of Physcomitrella strains collected from locations worldwide and carried out an initial assessment of natural variation in photoperiodic regulation. Using the first draft of the moss genome sequence, which was made available to me in the summer of 2006 as an annotator, I searched for moss sequences related to Arabidopsis genes of interest using the tblastn algorithm. Related sequences were aligned using the TCOFFEE algorithm, which also produced phylogenetic trees. In addition, I carried out a small side project using chemical genetics approaches to further elucidate the photoperiodic mechanism in Arabidopsis. Finally, I served as an additional senior scientist in the Coupland group, where I contributed my expertise and experience to the mentoring and training of graduate students and postdoctoral fellows at the MPI. Events. I attended weekly seminars by outside speakers, weekly meetings of the Coupland research group, weekly meetings of the Plant Development department, as well as the annual symposium held at the Max Planck Institute for Plant Breeding research. Services. As stated under activities, I served as an additional senior scientist in the Coupland group, where I contributed my expertise and experience to the mentoring and training of graduate students and postdoctoral fellows at the MPI. Dissemination: I presented this work several times to the Coupland research group, and presented research seminars for the Plant Biology department at the Max Planck, at the University of Freiburg, and at Kenyon College. PARTICIPANTS: Karen Hicks served as PI of this project. The Max Planck Institute for Plant Breeding Research was the partner organization and provided facilities, equipment, and supplies for this work. Collaborators and contacts include George Coupland, Bernd Reiss, and members of their laboratories. TARGET AUDIENCES: Other plant biology researchers and students in courses taught by Karen Hicks are the target audience. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
I gained in scientific expertise by learning new techniques that will allow me to carry out future investigations using moss as a model system, particularly through my interactions with the Reiss group at the MPI in Cologne, but also through interactions with the moss group at the University of Freiburg.
Publications
- Rensing, S.A., Lang, D., Zimmer, A.D., Terry, A., Salamov, A., Shapiro, H., Nishiyama, T., Perroudm P.F., Lindquist, E.A., Kamisugi, Y., Tanahashi, T., Sakakibara, K., Fujita, T., Oishi, K., Shin-I, T., Kuroki, Y., Toyoda, A., Suzuki, Y., Hashimoto, S., Yamaguchi, K., Sugano, S., Kohara, Y., Fujiyama, A., Anterola, A., Aoki, S., Ashton, N., Barbazuk, W.B., Barker, E., Bennetzen, J.L., Blankenship, R., Cho, S.H., Dutcher, S.K., Estelle, M., Fawcett, J.A., Gundlach, H., Hanada, K., Heyl, A., Hicks, K.A., Hughes, J., Lohr, M., Mayer, K., Melkozernov, A., Murata, T., Nelson, D.R., Pils, B., Prigge, M., Reiss, B., Renner, T., Rombauts, S., Rushton, P.J., Sanderfoot, A., Schween, G., Shiu, S.H., Stueber, K., Theodoulou, F.L., Tu, H., Van de Peer, Y., Verrier, P.J., Waters, E., Wood, A., Yang, L., Cove, D., Cuming, A.C., Hasebe, M., Lucas, S., Mishler, B.D., Reski, R., Grigoriev, I.V., Quatrano, R.S., Boore, J.L.. 2008. The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants. Science 319:64-69.
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