Source: UNIVERSITY OF FLORIDA submitted to NRP
MANAGEMENT OF DISEASES AFFECTING VEGETABLE AND ORNAMENTAL CROPS IN FLORIDA
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0207002
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Mar 1, 2006
Project End Date
Feb 28, 2010
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
AGRI RES & ED CENTER, APOPKA
Non Technical Summary
Managing plant disease is an important sub-component of plant protection for vegetable and ornamental production. As with insects, management of plant diseases present unique situations and opportunities, so disease management research is an important goal. The objective of this research is to explore and develop safe and effective disease management methods for vegetable and ornamental production in order to reduce impacts from diseases and reduce pesticide use. Information gained would also apply to horticultural crop production throughout Florida, southeastern U.S. and the Caribbean. Florida's horticultural industry is currently responding to new pests, foreign competition, expanding urbanization, environmental concerns, and other economic and social factors by shifting to different crop production systems and product diversification. The shift has been to novel, higher-quality, branded, and value-added products. Examples are ethnic vegetables, vine-ripened tomatoes and branded fruits such as strawberries, miniature vegetables, ripe products such as colored pepper, Cuban squash, and herbs such as cilantro (coriander). Organic vegetable production is also increasing. Nurserymen are planting new landscape cultivars and species and producing larger, higher quality plants suitable for 'instant landscapes'. These trends are creating new pest and production problems, so new approaches to production, plant protection are required.
Animal Health Component
75%
Research Effort Categories
Basic
25%
Applied
75%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2122110116030%
2122121116020%
2122123116020%
2122199116030%
Goals / Objectives
To develop disease management strategies to protect vegetables and ornamental plants from diseases or lessen their impact thereby increasing the profitability of production in an environmentally sound manner. Specific objectives include: 1. Describe and identify important diseases, pathogens, and disorders and study the interactions between plants and their pathogens that result in disease damage, including the evaluation, modification, and development of detection and identification techniques for plant pathogens. 2. Study the life systems of disease-producing organisms and plant production systems and identify important factors which can be manipulated or altered to avoid or reduce the impacts of plant diseases. 3. Develop and evaluate disease management tactics which are economical and environmentally-acceptable and which minimize disease impacts and enhance economic return.
Project Methods
In many cases, causal agents will be unknown and must be elucidated by isolation, identification and pathogenicity tests. Methods and procedures will be diverse and determined by the specific problem, but will generally follow approaches standard to plant pathology research for viruses, bacteria and fungi. For example, fungi would be isolated from diseased tissue, grown in axenic culture, and identified by microscopic examination. In many cases, molecular techniques such as PCR, biochemical profiles, and DNA fingerprinting and hybridization techniques would be employed for identification and detection. Plants would be inoculated with the suspect pathogen and its ability to produce the original disease confirmed. When appropriate, the mechanisms and occurrence of important impacts and losses will be documented (for example seasonal occurrence, disease intensity, spacial distribution), and economically important damage thresholds determined. Knowledge of the life systems of pathogens is essential to determine which production activities are conducive to disease and which can be manipulated or revised to deter disease occurrence or slow development. Similar knowledge is needed to formulate pesticide-based approaches particularly in regard to timing and placement. The biology of pathogens will be studied in the laboratory to determine key variables such temperature and pH, basic reproductive and dissemination strategies, and survivability and persistence capabilities(for example, persistence in soil, seeds, or plant debris) would be investigated. In greenhouses or controlled-environments, factors such as temperature and leaf wetness periods that influence infection and disease development will be determined, then verified in field experiments. In the field, dissemination of the pathogen and the influence of weather on disease development would also be examined. Environmental monitoring, spore trapping, and monitoring of disease development and progress by sampling or scouting are examples of methods used in this phase of research. Effective fungicides and bactericides will be identified through lab, greenhouse and field testing sometimes using potted plants or detached leaves. Optimum rates, application methods, and frequencies will be determined through field testing in small, replicated plots or in larger plots with grower participation. Cultural activities greatly influence the success or failure of disease control and are a key part of plant disease management. With knowledge gained, interactions of important cultural activities can be postulated and verified in field experiments. Development of monitoring and scouting methods and approaches to these tasks may be appropriate in many instances. Fungicide programs alone will not provide acceptable results unless well-integrated with cultural practices that avoid disease or are detrimental to disease progress. However, in the case of horticultural crops, the testing and labeling of fungicides or biologically-based pesticides will be needed, so cooperative work with chemical companies and IR-4 will likely be an important component of this project.

Progress 03/01/06 to 02/28/10

Outputs
OUTPUTS: Dr. Strandberg is no longer with University of Florida PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Dr. Strandberg is no longer with University of Florida

Publications

  • No publications reported this period


Progress 10/01/05 to 09/30/06

Outputs
Some new biopesticide products (and mixtures) with potential to suppress or control of plant pathogenic bacteria were evaluated.Two representative Xanthomonas spp. were evaluated on Prunus umbellata or P. incisa x campanulata cv. Okame, infected with Xanthomonas campestris pv pruni, and Wax myrtle (Myrica cerifera infected with a Xanthomonas species (currently being identified. Pseudomonas chicorii and P. marginalis were evaluated on Hibiscus (Hibiscus rosa-sinensis), and Hydrangea (Hydrangea quercifolia) respectively. An unknown Xanthomonas species (which is currently being identified) was isolated from some Hydrangea plants, so there was likely a mixed infection on this host. Of the nine treatments, only the Actinovate plus Tricon consistently suppressed disease, but the levels were not always significantly different than the controls or other treatments. The results did not determine if Tricon, Actinovate, or the combination was responsible for the results obtained. With the possible exception of the Rapsody-Kocide-Vital, BioPhos with chelated copper, and Kocide, none of the other treatments appeared to have significant potential for controlling the diseases evaluated in this experiment when applied at 14-day intervals. The occurrence, survival, and potential use as a biocontrol agent on foliage plants of some bacteriophages of Xanthomonas was investigated Useful phages were found in plantae, in the soil, the pot the plants were in, from the soil below the benches in greenhouses, and from irrigation canals. Several ornamental and foliage were visited and samples collected. Over 140 phage isolates have been collected that infect various species of Xanthomonas and Pseudomonas. Additionally, 50 different phages of Xanthomonas campestris pv. begoniae (Xcb)have been isolated. One phage was found to infect most strains of Xcb. Populations of bacteriophage applied to leaf surfaces as a biocontrol against Xanthomonas infection were found to decline rapidly under full sun, but less rapidly in nursery environments such as a shade house or glass or plastic greenhouse. A major factor involved in phage decline was ultra-violet light, in addition to near-infrared light. The current work involves optimizing application techniques for biocontrol as well as partially characterizing the phages of interest. The most useful phages were viewed with a transmission electron microscope. The morphology and size indicates they likely belong to the either the family Tectivirdae or Corticoviridae.

Impacts
The IR-4 Biopesticide project identified potential biopesticides to control bacterial diseases of landscape ornamentals which occur sporadically on woody ornamental during nursery production, but cause major production problems and losses. Bacteria in the genera Xanthomonas and Pseudomonas cause the greatest losses, particularly in humid climates. Diseases incited by Pseudomonas spp. such as P. syringae, P. chicorii, and P. marginalis occur frequently, and affect many nursery crops. Various pathovars of Xanthomonas campestris, are especially common, cause frequent epidemics especially in warm, humid regions), and are destructive pathogens on numerous ornamental species. Presently, there are few options for suppression once such diseases appear. Copper compounds and antibiotics provide mostly unsatisfactory results in disease-favorable climates. Although further work is needed, it can be focused on potential products identified in this work to provide materials more useful to nurserymen for control of bacterial plant pathogens. Bacteriophages are being used to suppress bacterial plant pathogens on several crops including greenhouse-grown tomatoes. There is significant potential for use of bacteriophages to control bacterial diseases of foliage crops and landscape ornamentals. Problems of identifying appropriate phages, extended survival in greenhouse environnments, identification of factors detrimental to survival, and improved application and use strategies are being investigated which should provide additional methods for use of bacteriophages for this application.

Publications

  • Strandberg, James 0., 2006. Evaluation of Some Bactericides and Biopesticides for Suppression of Bacterial Diseases on Landscape Ornamental Plants. University of Florida Institute of Food and Agricultural Sciences Mid-Florida Research and Education Center Plant Pathology Research Report 2006-2. 12 Pages.
  • Strandberg, James 0., 2006. Effects of mixtures of neutral salts of phosphorous acid with Copper and nickel chelates on disease development of Xanthomonas campestris pv. vesicatoria on tomato plants. University of Florida Institute of Food and Agricultural Sciences Mid-Florida Research and Education Center Plant Pathology Research Report 2006-2. 5 Pages.