Progress 05/01/06 to 12/31/06
A nitrate biosensor based on the enzyme nitrate reductase was formulated for agricultural applications. Screen printed carbon electrode (SPCE) for nitrate biosensor with NECi Superior Stock Nitrate Reductases (YNaR1 & AtNaR2) and using methyl viologen as electron carrier were developed. Formulation of SPCE-YNaR1 was done by varying enzyme drying method, selecting most effective and safe electrode-carrying dye, and establishing an effective sample reagent pack with buffer system containing sulfite for eliminating oxygen interference. The nitrate biosensor was used to analyze nitrate standards for calibration and nitrate content of real world samples extracted from corn leaves and soils. The resulting data demonstrated the feasibility of constructing a prototype nitrate biosensor for application in agriculture in Phase II. This device will assist farmers and agricultural consultants with nutrient management by providing rapid field analysis.
The impact on agriculture is expected to be by making it easier for farmers to evaluate nitrate content of plants and soils in the field with ease and very low cost per sample. This approach will make it simpler for farmers and agricultural consultants to determine the nitrogen fertilizer needs for agricultural fields.
- No publications reported this period