Progress 09/15/05 to 09/14/07
Outputs
OUTPUTS: This project has produced both male and female antisense ACC-oxidase (ACO-1) parental lines of Galia muskmelon as well as antisense (ACO-1) Galia F1 hybrids. These lines can be used to maintain the development of this new muskmelon. Through continued selection and traditional breeding efforts, a delayed-ripening Galia muskmelon could be a potential new product for growers. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
This project has successfully produced a transformed Galia muskmelon even though past research had deemed this melon recalcitrant to transformation. Information on Galia transformation as well as research on the aromatic profile of Galia has been documented through the work of this project. Markets for greenhouse grown hydroponic vegetables are strong throughout the U.S. These markets continue to increase with diversification of the commodities grown. The Galia muskmelon has brought billions of dollars to exporters of the fruit from Israel, Spain, Turkey, and Morocco, to Europe. Pilot sales in the U.S., including Publix supermarkets, has given similar potentials for fruit produced in the U.S. especially in warmer climates such as Florida and Puerto Rico. One of the major drawbacks to increased sales is soft fruit, as they must be harvested fully ripe for optimum flavor. The research completed in this project will produce a new melon line that will ripen at a slower rate and therefore be firmer longer. This will help growers to produce a superior Galia muskmelon with a longer postharvest shelf-life, ready for market.
Publications
- Nunez-Palenius, H.G., H.J. Klee, D.J. Huber and D.J. Cantliffe. 2007. Fruit ripening characteristics in a transgenic Galia male parental muskmelon (Cucumis melo L. var. reticulatus Ser.) line. Postharvest Biology and Technology 44 (2): 95-100.
- Mitchell, J.M., D.J. Cantliffe, H.J. Klee, S.A. Sargent, and P.J. Stoffella. 2007. Fruit quality characteristics of Antisense Galia F1 Hybrid muskmelon (Cucumis melo L. var. reticulatus Ser.). HortScience 42(4): 907.
- Nunez-Palenius, H.G., H.J. Klee, D.J. Huber and D.J. Cantliffe. 2007. A single insertion of ACC oxidase gene in antisense orientation extends the shelf life in muskmelon Galia hybrid parental line (Cucumis melo L. var. reticulatus Ser.). Acta Hort. 731:421-426.
- Mitchell, J.M., D.J. Cantliffe, H.J. Klee, S. A. Sargent, and P.J. Stoffella. 2007. Fruit quality characteristics of Galia F1 Hybrid (Cucumis melo L. var. reticulatus Ser.) muskmelon developed from a transgenic male parent. Acta Hort. 731:31-37.
- Nunez-Palenius, H.G., D.J. Cantliffe, D.J. Huber, J. Ciardi, and H.J. Klee. 2005. Transformation of a muskmelon Galia hybrid parental line (Cucumis melo L. var. Reticulatus Ser.) with an antisense ACC oxidase gene. Plant Cell Reports. 25: 198-205.
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Progress 09/15/05 to 09/15/06
Outputs
Production, yield and quality characteristics of an antisense ACC oxidase Galia F1 hybrid muskmelon. Transgenic male parental lines of Galia muskmelon bearing an antisense ACC oxidase (ACO-1) gene were developed from cotyledon explants cultured in vitro. Transgenic fruits from the ACO-1 antisense (AS) male line produced less ethylene and had a longer shelf-life compared to wild-type fruits. The other postharvest quality characteristics of the transgenic fruit were similar to wild-type fruit. The AS male lines were selfed and selected for the delayed ripening phenotype throughout 2005. A T4 population was produced. It was not possible to regenerate a transgenic (ACO-1) female Galia parental line thus; T0F1 pollen was backcrossed to the wild-type female Galia parental line in order to insert the antisense ACC oxidase (ACO-1) gene. The female transgenic backcross 1 (BC1) seeds were produced during the summer 2004. This work was continued throughout 2005 and a BC4
population was produced. During spring 2006, hybrid crosses were made with the transgenic (T4 male and BC4 female) parental lines. These hybrids are currently in production at the Plant Science Research and Education unit (PSREU) located at Citra, FL. They will be evaluated during fall, 2006. Lines expressing the delayed ripening phenotype will be further evaluated in spring 2007. At that time, post harvest storage research will be conducted to observe the potential of this hybrid as a new commodity.
Impacts
If the transgenic melons have equivalent postharvest quality and extended shelf life compared to the non-transgenic parent, the line will potentially be introduced into commerce. This would provide a new, high value crop for growers.
Publications
- Nunez-Palenius, H.G., D.J. Cantliffe, D.J. Huber, J. Ciardi, and H.J. Klee. 2006. Transformation of a muskmelon Galia hybrid parental line (Cucumis melo L. var. Reticulatus Ser.) with an antisense ACC oxidase gene. Plant Cell Reports 25(3) 198-205.
- Nunez-Palenius, H.G., D.J. Cantliffe, H.J. Klee, D.J. Huber. 2005. A single insertion of ACC Oxidase gene in antisense orientation extends the shelf life in muskmelon Galia hybrid parental line (Cucumis melo L. var. reticulatus Ser.). Acta Horticulturae. In press. Mitchell, J.M., D.J. Cantliffe, H.J. Klee, S.A. Sargent, and P.J. Stoffella. 2005. Fruit quality characteristics of Galia F1 Hybrid (Cucumis melo L. var. reticulatus Ser.) muskmelon developed from a transgenic male parent. Acta Horticulturae. In press.
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