Progress 09/15/05 to 09/14/09
Outputs
The proposal objectives were achieved or exceeded with a one year no cost extension to the original timeline. The course Understanding Biotechnology (BIO495) was developed and taught in spring semester 2007, fall semester 2007 and fall semester 2008 (21 total students). Laboratory experiments included DNA electrophoresis, digestion, transformation, Western blotting, DNA isolation, and PCR. An Advanced Molecular Techniques course was also developed and offered in the Spring Semester of 2008 and Spring 2009 (6 students). The students completed experiments using an ABI3130 Genetic Analyzer to analyze DNA fragments, Arabidopsis transformation, and used various bioinformatics databases. Two molecular biology experiments were incorporated into the Cell Biology Laboratory section in the Fall 2006, 2007, and 2008 semesters which impacted over 60 students. Additionally, the authors of this grant assisted the KSU Biology Faculty to plan a new Biotechnology Option for undergraduate Biology Majors, which included the courses developed by this grant. The Cell Biology, Understanding Biotechnology (now BIO 432: Biotech II: DNA) and Advanced Biotechnology (now BIO 434: Biotech IV: Advanced Biotechnology) courses are now required classes for the Biotechnology Option. Through this grant, nine undergraduate students conducted research projects concerning pawpaw molecular genetics and also earned course credit for their projects. Eight students made presentations at KAS Meetings in 2007-2009 and at Posters-at-the-Capitol in 2007-2009. Seven students presented their research project results at KSU senior seminar on campus. In an effort to support the recruitment of undergraduate and graduate students to KSU in biotechnology, there were recruiting visits to high schools, development of a biotechnology website, and increased KSU library holdings in biotechnology. A workshop was held for high school students in the Research and Extension Apprenticeship Program which included a hands on gel electrophoresis laboratory. Students were recruited at a Community and Technical College for the KSU Biology and Biotechnology programs. Biotechnology program brochures were distributed in area high schools during recruiting visits by an aquaculture educator, a STEM open house, and The College Reality Store on the KSU campus, providing information to over 100 prospective KSU students. A Biotechnology and DNA Forensics Workshop was held in June 2007-2009 for 150 7th grade students in the KSU Pathways and Access to Careers in Technology Program. Students for the course BIO 495: Understanding Biotechnology were recruited via the biotech website, KSU Campus Announcements, fliers placed around KSU's campus, and announcements via the KSU public relations department. Biotechnology brochures were distributed at a number of campus events and high schools. The KSU Biotechnology website (http://biotech.kysu.edu) was created with information about the Biotechnology courses and Option, as well as research projects conducted by KSU students. There have been over 1038 hits on the website since April 10, 2007. Seven books were purchased by the grant and added to the KSU library collection. PRODUCTS: Two biotechnology courses were developed and offered through support by this grant. Two biotechnology laboratory experiments were incorporated into the Cell Biology course. Eight undergraduate students conducted research projects with materials and equipment purchased by the grant. The KSU Biotechnology website was updated during the spring of 2008 and can be found at http://biotech.kysu.edu. A Biotechnology brochure was printed and distributed that included the three biotech courses supported by the grant. This brochure was distributed at a number of events and high schools. OUTCOMES: Understanding Biotechnology, Advanced Techniques in Biotechnology, and Cell Biology students learned biotechnology techniques in laboratory sessions. Undergraduate student researchers learned DNA based molecular techniques used in biotechnology and made presentations concerning their research results at scientific meetings. A Biotechnology Option for Kentucky State University Biology majors was developed with support from this grant. Efforts to recruit students for the biotech curriculum were initiated through the development of a biotechnology website, a CD, and distribution of a KSU Biotechnology brochure to assist in recruiting efforts. Students in the Pathways and Access to Careers in Technology summer program participated in a Biotechnology and DNA Forensics Workshops for 7th grade students at KSU that was supported by this grant. Partnerships and collaborative ventures in biotechnology teaching and undergraduate research opportunities were created with Dr. Liedl, West Virginia State University, Dr. Hildebrand, University of Kentucky, Dr. Robinson, Bellarmine University, Dr. Bigdeli, KSU Chair for Mathematics and Sciences Division and other KSU participants, as well as Dr. Dinkins at USDA ARS FAPRU in Lexington, KY. DISSEMINATION ACTIVITIES: Information concerning the development of a biotechnology curriculum and undergraduate student research opportunities in biotechnology techniques were disseminated through a biotechnology website. A CD and a brochure concerning the KSU biotechnology program were distributed at a number of KSU events and high schools to assist in recruiting efforts. Students in the Pathways and Access to Careers in Technology summer program participated in a Biotechnology and DNA Forensics Workshop for fifty 7th grade students at KSU that was supported by members of this grant. Eight students made a presentation at the Kentucky Academy of Science Meeting in 2007-2009 and at Posters-at-the-Capitol in 2007-2009 concerning their research projects. A total of 16 abstracts and journal articles were published from research efforts by students who participated in research efforts supported by this grant. FUTURE INITIATIVES: There have been discussions with several biotechnology companies in Kentucky in an effort to continue funding for the future instruction of biotechnology courses at KSU. Additional laboratory fees are being considered to support the instruction of the biotechnology laboratory exercises. This grant has positively impacted the Project PD(s), students, and KSU collaborators by enhancing opportunities for grant funding, research experiences, new equipment, and training of students in molecular biology techniques that allowed the students to pursue careers in agriculture and the life sciences.
Impacts
Although the U.S. economy has been fluctuating over the last few years, the number of biotechnology jobs has increased, thereby indicating the critical need to train students in biotechnology techniques who wish to pursue careers in agriculture and the life sciences. Students participated in classes and laboratory training in the techniques of biotechnology and also received hands on training in these techniques through undergraduate research projects. These students were prepared for graduate school and employment opportunities in the areas of molecular biology and biotechnology. A Biotechnology Option for Kentucky State University Biology majors was developed with support from this grant. A biotechnology website was created (http://biotech.kysu.edu) to support instruction of biotechnology courses and to recruit students for the biotechnology curriculum at KSU for undergraduate Biology and Masters of Aquaculture programs. With the initiation of this biotechnology curriculum, the number of undergraduate biology major students enrolled at KSU is expected to increase.
Publications
- Pomper, Kirk W., Jeremiah D. Lowe, Li Lu, Sheri B. Crabtree, and Lauren A. Collins. 2009. Clonality of Pawpaw (Asimina triloba) Patches in Kentucky. Journal of the Kentucky Academy of Science. 70:103.
- Lu, Li, Kirk W. Pomper, Karan Kaul, Narayanan Rajendran, and James Tidwell. 2009. Molecular Biology and Biotechnology Courses and Opportunities at Kentucky State University. Journal of the Kentucky Academy of Science. 70:103.
- Pomper, Kirk W., Jeremiah D. Lowe, Li Lu, Sheri B. Crabtree, and Lauren A. Collins. 2009. Clonality of Pawpaw (Asimina triloba) Patches in Kentucky. Journal of The Kentucky Academy of Sciences. 70:3-11.
- Pomper, Kirk, Jeremiah Lowe, Li Lu, Sheri Crabtree, Shandeep Dutta, Kyle Schneider, and James Tidwell. 2009. Assessment of Genetic Diversity of Pawpaw (Asimina triloba) Cultivars with Simple Sequence Repeat Markers. HortScience 44:1174-1175.
- Dutta, Shandeep, Kirk Pomper, Li Lu, Jeremiah Lowe, and Kyle Schneider, 2009. Determining the Utility of Simple Sequence Repeat Markers for Examining Genetic Relationships in Pawpaw Varieties. Posters at the Capitol Abstract Book pg. 27.
- Lowe, Jeremiah, Shandeep Dutta, Li Lu, Kirk Pomper, Sheri Crabtree, and Kyle Schneider. 2009. Pawpaw Cultivar Fingerprinting and Progeny Determination Using Simple Sequence Repeat Markers. HortScience 44:1174.
- Lu, L., K. W. Pomper, K. Kaul, N. Rajendran, and J. Tidwell. 2009. Introducing Modern Molecular Biology and Biotechnology to Students and Public in Kentucky Areas. Association of Research Directors Program and Abstracts, pg. 183.
- Dutta, S. J., L. Lu, J. D. Lowe, K. W. Pomper and K. Schneider. 2009. Development of Simple Sequence Repeat Markers for Examining Genetic Relationships in Pawpaw Varieties. Association of Research Directors Program and Abstracts, pg. 183.
|
Progress 09/15/07 to 09/14/08
Outputs
1) Support the instruction and development of the BIO495/AQU 595 courses (a) Understanding Biotechnology and (b) Advanced Techniques in Biotechnology. The course Understanding Biotechnology was taught by Dr. Pomper in Fall 2007 (6 students) and Fall 2008 (5 students). Undergraduate students learned about biotechnology through lectures and gained hands on experience in laboratories with the methods of molecular biology used in biotechnology. The Advanced Techniques course was taught by Dr. Li Lu in the Spring Semester of 2008 (4 students). 2) Enhance the laboratory experiences of the course Cell Biology with new molecular techniques. Dr. Kaul continued incorporating two molecular biology experiments in his Cell Biology Laboratory section in the Fall 2007 and Fall 2008 semesters. 3) Support a number of individual undergraduate student research projects in biotechnology for those who have completed the biotechnology courses. Two undergraduate students conducted research projects concerning pawpaw molecular genetics and also earned course credit for their projects. A student made a presentation at the Kentucky Academy of Science Meeting in 2007 and at Posters-at-the-Capitol in 2008 concerning their research project. One student presented the results of her research project at her senior seminar on campus. 4) Support the recruitment of undergraduate and graduate students to KSU in biotechnology through recruiting visits to high schools, development of a biotechnology website, and increase the KSU library holdings of reference books concerning biotechnology. A workshop was held for high school students in the Research and Extension Apprenticeship Program (REAP) which included a hands on gel electrophoresis laboratory. Dr. Lu recruited students on the campus of Bluegrass Community and Technical College to encourage community college students to continue their education at Kentucky State University in the areas of Biology and Biotechnology. Brochures have distributed in several high schools during recruiting visits by an aquaculture educator. A Biotechnology and DNA Forensics Workshop was held in June for fifty 7th grade students in the KSU Pathways and Access to Careers in Technology Program. Students for the course BIO 495: Understanding Biotechnology were recruited via the biotech website, KSU Campus Announcements, fliers placed around KSUs campus, and announcements via the KSU public relations department. A biotechnology brochure was distributed at a number of campus events and high schools. The KSU Biotechnology website was updated with information on the fall semester of BIO 495: Understanding Biotechnology. There have been over 613 hits on the website since April 10, 2007. In the summer of 2008, two high school students learned molecular biology techniques and completed a project isolating DNA from pawpaw leaves and running PCR to conduct studies on native pawpaw patches. These students were also part of the REAP summer program. Seven books were purchased and added to the library collection. PRODUCTS: Two undergraduate students conducted research projects with materials and equipment purchased by the grant. Two biotechnology laboratory experiments were incorporated into the Cell Biology course. The KSU Biotechnology website was updated during the spring of 2008 and can be found at http://biotech.kysu.edu. A Biotechnology brochure was printed and distributed that included the three biotech courses supported by the grant. This brochure was distributed at a number of events and high schools. OUTCOMES: Understanding Biotechnology, Advanced Techniques in Biotechnology, and Cell Biology students learned biotechnology techniques in laboratory sessions. Undergraduate student researchers learned some of the molecular techniques used in biotechnology and made presentations concerning their research results at scientific meetings. Efforts to recruit students for the biotech curriculum were initiated through the development of a biotechnology website, a CD, and distribution of a KSU Biotechnology brochure to assist in recruiting efforts. DISSEMINATION ACTIVITIES: Information concerning the development of a biotechnology curriculum and undergraduate student research opportunities in biotechnology techniques were disseminated through a biotechnology website. A brochure concerning the KSU biotechnology program was distributed at a number of KSU events and high schools to assist in recruiting efforts. Students in the Pathways and Access to Careers in Technology summer program participated in a Biotechnology and DNA Forensics Workshop for fifty 7th grade students at KSU that was supported by members of this grant. FUTURE INITIATIVES: There have been discussions with several biotechnology companies in Kentucky in an effort to continue funding for the future instruction of biotechnology courses at KSU.
Impacts
Although the U.S. economy has been fluctuating over the last year, the number of biotechnology jobs has increased over the last several years, thereby indicating the critical need to train students in biotechnology techniques who wish to pursue careers in agriculture and the life sciences. Students participated in classes and laboratory training in the techniques of biotechnology and also received hands on training in these techniques through undergraduate research projects. A biotechnology website was created (http://biotech.kysu.edu) to support instruction of biotechnology courses and to recruit students for the biotechnology curriculum at KSU for undergraduate Biology and Masters of Aquaculture programs. With the initiation of this biotechnology curriculum, the number of undergraduate biology major students enrolled at KSU is expected to increase.
Publications
- Collins, Lauren A., Li Lu, Jeremiah D. Lowe, Sheri B. Crabtree, and Kirk W. Pomper. 2008. Assessment of Clonality in Native Kentucky Pawpaw Patches Using Intersimple Sequence Repeat Markers. Journal of the Kentucky Academy of Science. 69:74
- Lu, Li, Kirk W. Pomper, Karan Kaul, Narayanan Rajendran, and James Tidwell. 2008. Development of Biotechnology Courses to Enhance Aquaculture and Biology Programs and Recruit Students to Kentucky State University. Journal of the Kentucky Academy of Science. 69:74
- Collins, Lauren A., Kirk Pomper, Li Lu, Jeremiah Lowe, and Sheri Crabtree. 2008. Assessment of Clonality in Native Kentucky Pawpaw Patches Using DNA Markers. Seventh Annual Posters at the Capitol Program Booklet. Page 32; available at http://campus.murraystate.edu/services/ursa/Posters_2008_Booklet.pdf
|
Progress 09/15/06 to 09/14/07
Outputs
1) Support the instruction and development of the BIO495/AQU 595 courses (a) Understanding Biotechnology and (b) Advanced Techniques in Biotechnology. The course Understanding Biotechnology was listed in the 2007 Fall Course Catalog and was taught by Dr. Pomper in the Spring (10 students) and Fall (6 students) Semesters of 2007. Undergraduate students learned about biotechnology through lectures and gained "hands on" experience in laboratories with the methods of molecular biology used in biotechnology. The Advanced Techniques course will be taught by Dr. Li Lu in the Spring Semester of 2008. 2) Enhance the laboratory experiences of the course Cell Biology with new molecular techniques. Dr. Kaul continued incorporating two molecular biology experiments in his Cell Biology Laboratory section in the Fall 2006 and Fall 2007 semesters. 3) Support a number of individual undergraduate student research projects in biotechnology for those who have completed the biotechnology
courses. Three undergraduate students conducted research projects concerning pawpaw molecular genetics. Two students worked on a project concerning pawpaw SSRs and various electrophoreses methods. One student made a presentation at KAS in 2006 and at Posters-at-the-Capitol 2007 concerning her research. After taking BIO 495 in spring 2007, an additional student continued a summer internship focusing on training in molecular marker techniques. 4) Support the recruitment of undergraduate and graduate students to KSU in biotechnology through recruiting visits to high schools, development of a biotechnology website, and increase the KSU library holdings of reference books concerning biotechnology. On June 11 and 12, 2007 the Pathways and Access to Careers in Technology summer program held a Biotechnology and DNA Forensics Workshop for fifty 7th grade students at KSU that was supported by members of this grant. At the Science, Technology, Engineering, and Math open house, The College
Reality Store, in Carver Hall on Saturday, April 14th, 2007, information about KSU biotechnology opportunities was provided to over 100 prospective KSU students. Students for the course BIO 495: Understanding Biotechnology were recruited via the biotech website, KSU Campus Announcements, fliers placed around KSUs campus, and the Kentucky state employee email system. A biotechnology brochure was developed that included the three biotech courses supported by the grant. This brochure was distributed at a number of events. The KSU Biotechnology website was updated with information on the fall semester of BIO 495: Understanding Biotechnology. There have been over 240 hits on the web site since April 10, 2007. In the summer of 2007, two high school students learned molecular biology techniques and completed a project isolating DNA from pawpaw leaves and running PCR to conduct studies on native pawpaw patches. These students were part of the REAP summer program. A copy of the book
Biotechnology: An Introduction, by Susan R. Barnum, Publisher: Brooks Cole; Updated 2nd edition (2005) was purchased for use in the Understanding Biotech course and will be added to the library collection.
PRODUCTS: Three undergraduate students were hired and research projects conducted with materials and equipment purchased by the grant. Two biotechnology laboratory experiments were incorporated into the Cell Biology course. The KSU Biotechnology website was updated during the spring of 2007 and can be found at http://biotech.kysu.edu. A KSU CD and Biotechnology brochure were developed that included the three biotech courses supported by the grant. This brochure was distributed at a number of events.
OUTCOMES: Cell Biology and Understanding Biotechnology students learned biotechnology techniques in laboratory sessions. Undergraduate student researchers learned some of the molecular techniques used in biotechnology and made presentations concerning their research results at scientific meetings. Efforts to recruit students for the biotech curriculum were initiated through the development of a biotechnology web site, a CD, and distribution of a KSU Biotechnology brochure to assist in recruiting efforts.
DISSEMINATION ACTIVITIES: Efforts to disseminate information concerning the development of a biotechnology curriculum and offer undergraduate student training in biotechnology techniques were initiated through the development of a biotechnology web site and a CD. A brochure concerning the KSU biotechnology program was developed and distributed at a number of events to assist in recruiting efforts. Students in the Pathways and Access to Careers in Technology summer program participated in a Biotechnology and DNA Forensics Workshop for fifty 7th grade students at KSU that was supported by members of this grant. In the Science, Technology, Engineering, and Math open house, The College Reality Store, in Carver Hall on Saturday, April 14th, 2007, provided information about Land Grant Biotechnology Opportunities to over 100 prospective KSU students.
FUTURE INITIATIVES: Several biotechnology companies have been identified in Kentucky and preliminary discussions have begun concerning whether funding from these companies could partially support instruction of biotechnology courses in the future.
Impacts
The number of biotechnology jobs has increased greatly in the last 10 years, thereby indicating the critical need to train students in biotechnology techniques who wish to pursue careers in agriculture and the life sciences. Students participated in classes and laboratory training in the techniques of biotechnology and also received hands on training in these techniques through undergraduate research projects. A biotechnology web site was created (http://biotech.kysu.edu) to support instruction of biotechnology courses and to recruit students for the biotechnology curriculum at KSU for undergraduate Biology and Masters of Aquaculture programs. With the initiation of this biotechnology curriculum, the number of undergraduate biology major students enrolled at KSU is expected to increase.
Publications
- Lowe, J. D., K. Long, and K.W. Pomper. 2007. Using Simple Sequence Repeat Markers to Distinguish Scion and Rootstock Tissues on Grafted Pawpaw Trees. Journal of the Kentucky Academy of Science 68:102.
- Collins, L.A., J.D. Lowe, and K.W. Pomper. 2007. A Comparison of Agarose, Metaphor Agarose, and Polyacrylamide Gel Electrophoresis Systems in Resolving Pawpaw Simple Sequence Repeat Markers Journal of the Kentucky Academy of Science 68:102-103.
- Collins, L.A., J.D. Lowe and K.W. Pomper. 2007. A Comparison of Agarose, Metaphor Agarose, and Polyacrylamide Gel Electrophoresis Systems in Resolving Pawpaw Molecular Markers Using Molecular Markers to Determine Clonality of Native Pawpaw Patches. Posters-At-The Capitol Abstracts Book, p. 50.
|
Progress 09/15/05 to 09/15/06
Outputs
Progress report by grant objective for 09/15/2005 to 09/15/2006: 1) Support the instruction and development of the BIO495/AQU 595 courses (a) Understanding Biotechnology and (b) Advanced Techniques in Biotechnology. The course Understanding Biotechnology was listed in the 2006 Fall Course Catalog and will be taught by Dr. Pomper in the Spring Semester of 2007. The Advanced Techniques course will be taught by Dr. Rajendran in the Fall Semester of 2007. Additionally, the authors of the grant participated in a series of meetings with the KSU Biology Faculty to plan a new Biotechnology Option for undergraduate Biology Majors. These meetings resulted in the submission of the Biotechnology curriculum for approval by the KSU Curriculum Committee and the KSU Faculty Senate in May, 2006. The Cell Biology, Understanding Biotechnology and Advanced Biotechnology courses in this grant will be required classes for the Biotechnology Option. The Biotechnology Option will start in
Fall of 2007. 2) Enhance the laboratory experiences of the course Cell Biology with new molecular techniques. Dr. Kaul incorporated two molecular biology experiments in his Cell Biology Laboratory section in the Fall 2005 semester. In the first experiment, students determine the length of an unknown DNA molecule by comparing its electrophoretic mobility with six DNA molecules of known size. In the second experiment, students digested lambda DNA using the restriction endonucleases EcoR1 and BamH1 in order to identify specific sites, sequences, and structures along the phage genome. 3) Support a number of individual undergraduate student research projects in biotechnology for those who have completed the biotechnology courses. Two undergraduate students conducted research projects concerning pawpaw molecular genetics. An undergraduate student used microsatellite markers to determine if pawpaw patches are clonal in the wild. The student presented her findings at two scientific meetings
and a senior seminar. A second undergraduate student conducted a study to evaluate the genetic diversity exhibited among 11 pawpaw cultivars. The student presented her findings at scientific meeting and a senior seminar. 4) Support the recruitment of undergraduate and graduate students to KSU in biotechnology through recruiting visits to high schools, development of a biotechnology website, and increase the KSU library holdings of reference books concerning biotechnology. Job advertisements were distributed for the Co-Investigator of Biotechnology Position, which will support instruction of the biotech classes, assist in undergraduate research projects, and assist in recruiting students to KSU. The KSU Biotechnology website was created and includes Biotechnology courses offered at KSU, careers in Biotechnology information, and KSU undergraduate research opportunities, and can be found at http://biotech.kysu.edu. A wallet sized CD was created that contains past biotechnology
undergraduate research projects at KSU. The CD was distributed at the Association of Research Directors meeting in Atlanta in April, 2006.
PRODUCTS: Two biotechnology laboratory experiments were incorporated into the Cell Biology course. Two undergraduate students were hired and research projects conducted with materials and equipment purchased by the grant. The KSU Biotechnology website was created during the spring of 2006 and can be found at http://biotech.kysu.edu. About 500 copies of a wallet sized CD were created that contained past biotechnology research projects here at KSU, including undergraduate posters and presentations. A draft of a brochure was developed that included the three biotech courses supported by the grant.
OUTCOMES: Cell Biology students learned biotechnology techniques in laboratory sessions. Undergraduate student researchers learned some of the molecular techniques used in biotechnology and made presentations concerning their research results at scientific meetings. Efforts to recruit students for the biotech curriculum were initiated through the development of a biotechnology web site, a CD, and the hiring of a Co-Investigator of Biotechnology who will assist in recruiting efforts.
DISSEMINATION ACTIVITIES: Efforts to disseminate information concerning the development of a biotechnology curriculum and offer undergraduate student training in biotechnology techniques were initiated through the development of a biotechnology web site and a CD. The course Understanding Biotechnology was listed in the 2006 Fall Course Catalog and will be taught by Dr. Pomper in the Spring Semester of 2007. A draft of a brochure was developed that includes the three biotech courses supported by the grant.
FUTURE INITIATIVES: A number of biotechnology companies have been identified in Kentucky and preliminary discussions have begun concerning whether funding from these companies could partially support instruction of biotechnology courses in the future.
Impacts
The number of biotechnology jobs has increased greatly in the last 10 years, thereby indicating the critical need to train students in biotechnology techniques who wish to pursue careers in agriculture and the life sciences. Students participated in classes and laboratory training in the techniques of biotechnology and also received hands on training in these techniques through undergraduate research projects. A biotechnology web site was created (http://biotech.kysu.edu) to support instruction of biotechnology courses and to recruit students for the biotechnology curriculum at KSU for undergraduate Biology and Masters of Aquaculture programs. With the initiation of this biotechnology curriculum, the number of undergraduate biology major students enrolled at KSU is expected to increase.
Publications
- Dawson, Kelly, Jeremiah D. Lowe, Kirk W. Pomper. 2006. Using Simple Sequence Repeat Markers to Determine Clonality in Pawpaw Patches. Association of Research Directors, Inc. 14th Biennial Research Symposium Program and Abstracts Book, p. 169.
- Yeboah, Sybil, Jeremiah D. Lowe, and Kirk W. Pomper. 2006. Evaluation of Genetic Variation in Eleven Pawpaw Cultivars Using Simple Sequence Repeat Markers. Association of Research Directors, Inc. 14th Biennial Research Symposium Program and Abstracts Book, p. 46.
|
|