Source: WEST VIRGINIA STATE UNIVERSITY submitted to NRP
MAKING GENES COME TRUE: INFUSION OF INQUIRY USING MOLECULAR DIAGNOSTICS TO ADDRESS PLANT, ANIMAL AND HUMAN HEALTH ISSUES AND US BIOSAFETY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0204820
Grant No.
2005-38820-16354
Cumulative Award Amt.
(N/A)
Proposal No.
2005-03522
Multistate No.
(N/A)
Project Start Date
Sep 1, 2005
Project End Date
Aug 31, 2009
Grant Year
2005
Program Code
[EP]- Teaching Project
Recipient Organization
WEST VIRGINIA STATE UNIVERSITY
PO BOX 1000
INSTITUTE,WV 25112
Performing Department
BIOLOGY
Non Technical Summary
There is an explosion of genomic based technologies for use in molecular diagnostics. There is a paucity of individuals trained for careers in the areas of genomics. This is especially true of minority student populations. Under the umbrella of MAKING GENES COME TRUE, a team of scientists with wide range of biological research expertise will collectively offer supervision, guidance and direct participation to fill the gap of awareness that currently exists between minority schools and cutting edge science
Animal Health Component
40%
Research Effort Categories
Basic
40%
Applied
40%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2012499104020%
2022499106040%
2067410103010%
3043199104010%
7213199104010%
7237410104010%
Goals / Objectives
WVSUs concept is to assimilate and strengthen the institutions the existing proficiency in molecular diagnostics, animal population biology and systematics, model organisms/genomic resources, and plant biology. Specifically, our intent is to: 1) Design and implement a new dual level, undergraduate/graduate course, Introduction to Molecular Diagnostics; 2) Organize special workshops for faculty, undergraduate or graduate students, and K-12 level science teachers for extensive training in molecular diagnostics; 3) Intensify development of faculty research and teaching proficiency in the field of molecular diagnostics at WVSU and across participating 1890 Land-Grant universities by providing sequencing and genotyping services on a no-cost basis; 4) Develop creative thinking and infuse inquiry into student experimental learning by encouraging students to take up independent projects; and 5) Develop an internet resource to support student experimental learning related to human/animal/plant health topics, thereby augmenting efforts for independent study for teaching and research, and highlighting capacity-building efforts in genomics at WVSU.
Project Methods
The overall MAKING GENES COME TRUE program was conceived to bring together and strengthen existing expertise in DNA diagnostics and plant, animal and human health issues at WVSU and other 1890 Land-Grant universities. We have developed a course in molecular diagnostics that address this need. Upon completion of this 4-credit course of combined lecture and laboratory, students will be able to describe current molecular approaches to disease diagnostics and screening. We are proposing a workshop to be held at all the participating 1890 land grant universities. The workshop is intended for researchers, students and teachers. The participants will learn isolation/quantification of genomic DNA/RNA for various organisms, basics of PCR and DNA amplification, and basics of electrophoresis. This workshop will be centered on a variety of DNA diagnostic technologies, such as Single Nucleotide Polymorphisms (SNPs), microsatellites, AFLPs and PCR based RFLP, Real Time PCR, molecular beacons for disease diagnostics and bio-safety. The course is designed for students and researchers in genetics/molecular biology/plant breeding that have successfully completed Basic DNA LAB workshop. The participants learn the basic principles and uses of different DNA diagnostics with special reference to plant, animal and human diseases. A main thrust of this proposal is to set up a servicing sequencing and genotyping center that satisfies the research needs of the entire 1890 land-grant system. Researchers, teachers and graduate researchers from any of the 1890 land-grant university will be encouraged to submit new or expanded research proposals that incorporate aspects of DNA diagnostics. The faculty associated with MAKING GENES COME TRUE will review these proposals and extend genotyping and sequencing services at no cost. This objective is designed to encourage students and faculty at 1890 institutions to incorporate a genomic focus into their research. The mission of MAKING GENES COME TRUE is to improve classroom laboratory curricula. Our goal is to allow students to develop a biological question and then we will help him/her by devising series of experiments that would enable them to gather evidence to support or refute their hypothesis. New assessments will be created with an eye to go beyond this level and perhaps approach Application, Analysis, and Synthesis. We strive to create structured learning teams that experience active and cooperative inquiry during their independent research projects. Based on successful completion of an independent project, six students from participating 1890 land grant universities will be selected for summer internships at Dr.Molly Jahn laboratory at Cornell University. The students will receive intensive training in cutting edge molecular research. A set of web pages will be developed at WVSU for MAKING GENES COME TRUE, with the links to all participating universities. These web pages will communicate/disseminate all aspects of MAKING GENES COME TRUE.

Progress 09/01/05 to 08/31/09

Outputs
Workshops As planned, two workshops related to genomics were held at WVSU in the summers of 2006 and 2007. MGCT also supported, in part, a third workshop related to genomics at WVSU in 2008. A total of over 60 participants attended these workshops. Student training MGCT funds were used to support student research for seven students at WVSU. Three of these students completed thesis research supported in part by MGCT. Five students completed MGCT internships at Cornell University. PRODUCTS: Workshop manuals and CDs were prepared for the 2006 and 2007 workshops and made available to participants. These are available to others upon request. OUTCOMES: Of the three of the students supported as MGCT interns, one is now a research associate at the Smithsonian Institution, another is a PhD. Candidate at UNLV, and the third is a law student at WVU. Three of the students who completed internships at Cornell University are either entered graduate school in an area related to genomics, or plan to do so. Twenty students have received training in DNA sequencing using chemistry for analysis on the ABI 3130 analyzer purchased with MGCT funds. DISSEMINATION ACTIVITIES: Workshop manuals and CDs were prepared for the 2006 and 2007 workshops and made available to participants. These are available to others upon request. FUTURE INITIATIVES: This program is planned to continue and expand.

Impacts
60 students had a unique opportunity to be exposed to new techniques in the field of genomics. All 7 of the funded interns have used their training as part of their continued education.

Publications

  • No publications reported this period


Progress 09/01/07 to 08/31/08

Outputs
In Year 06-07, several projects related to MGCT were completed. In Fall, 06, Ms. Robin Turner completed her Master's thesis. In Spring, 2007, Ms. Jooha Jeong completed her Master's thesis. Both graduate student projects were supported in part by MGCT. In Fall, 2006, Dr. Umesh Reddy held a MAP Plant workshop that was also sponsored in part by MGCT. There were 22 workshop participants. Dr. Reddy's travel to the Land Grant Initiative for Genomics (sponsored by CSREES)was supported by MGCT. In January, 2007, all 1890 institutions were contacted by emailing an attached letter regarding the research opportunities offered to students and faculty at 1890 schools. In June, 2007, a second DNA workshop was held at West Virginia State University. There were 12 participants at this workshop. MGCT is providing sequence analysis services free of charge to all WVSU scientists working on DNA sequencing projects. PRODUCTS: A CD containing the workshop manual, in addition to DNA sequence files and information on obtaining DNA analysis freeware, was produced and provided to workshop participants. OUTCOMES: At present, MGCT funding has contributed to the training of 12 WVSU graduate students, and five WVSU undergraduate students. DISSEMINATION ACTIVITIES: An article on the DNA workshop was published in WVSU's Land-Grant Quarterly. FUTURE INITIATIVES: Dr. Reddy and the PI have discussed extending the idea of MGCT to other capacity building initiatives that will focus on training of undergraduate students in genomics.

Impacts
In the second year of the MGCT project, research project opportunities were provided to 25 students and faculty. Educational opportunities via workshop attendance was provided to 34 high school teachers and other educators.

Publications

  • DNA workshop manual No.2, 2007: produced for workshop participants.


Progress 09/01/06 to 08/31/07

Outputs
In Year 06-07, several projects related to MGCT were completed. In Fall, 06, Ms. Robin Turner completed her Master's thesis. In Spring, 2007, Ms. Jooha Jeong completed her Master's thesis. Both graduate student projects were supported in part by MGCT. In Fall, 2006, Dr. Umesh Reddy held a MAP Plant workshop that was also sponsored in part by MGCT. There were 22 workshop participants. Dr. Reddy's travel to the Land Grant Initiative for Genomics (sponsored by CSREES)was supported by MGCT. In January, 2007, all 1890 institutions were contacted by emailing an attached letter regarding the research opportunities offered to students and faculty at 1890 schools. In June, 2007, a second DNA workshop was held at West Virginia State University. There were 12 participants at this workshop. MGCT is providing sequence analysis services free of charge to all WVSU scientists working on DNA sequencing projects. PRODUCTS: A CD containing the workshop manual, in addition to DNA sequence files and information on obtaining DNA analysis freeware, was produced and provided to workshop participants. OUTCOMES: At present, MGCT funding has contributed to the training of 12 WVSU graduate students, and five WVSU undergraduate students. DISSEMINATION ACTIVITIES: An article on the DNA workshop was published in WVSU's Land-Grant Quarterly. FUTURE INITIATIVES: Dr. Reddy and the PI have discussed extending the idea of MGCT to other capacity building initiatives that will focus on training of undergraduate students in genomics.

Impacts
In the second year of the MGCT project, research project opportunities were provided to 25 students and faculty. Educational opportunities via workshop attendance was provided to 34 high school teachers and other educators.

Publications

  • DNA workshop manual No.2, 2007: produced for workshop participants.


Progress 09/01/05 to 09/01/06

Outputs
The following actions/activities were completed during Year 1 of MCGT: 1. An ABI 3130 genetic analyzer was purchased and located in a dedicated room in Hamblin Hall on the WVSU campus. The machine is fully functional. In addition, an incubator and PCR system were purchased using MGCT funds. 2. WVSU's Basic DNA workshop was held June 19-23 on the Institute campus. Participants received hand-on experience in genomic extraction, PCR amplification and clean-up, analysis of variable DNA markers, and analysis of DNA sequence. Three WVSU faculty (Ruhnke, Reddy and Huber) led the workshop, and 19 individuals participated. WVSU graduate student Robin Turner assisted in lab preparations. 3. Ms. Tiffany Blair participated in a MGCT research internship at Cornell University. Ms. Adrienne Thornburg completed training as a MGCT research assistant at WVSU. 4. Dr. Joshi from Fort Valley State University augmented a project on genetics of medicinal plants by completed AFLP fingerprint 4. A web site for the activities of the grant is available at http://fozzy.wvstateu.edu/mgct/. 5. A poster describing MGCT was presented at the ARD meetings in Atlanta, GA in spring of 2006. PRODUCTS: A manual for the Basic DNA workshop was completed and made available to the participants. OUTCOMES: Individuals ranging from middle school students to university professors were provided a range of training levels in genomics and biotechnology during the workshop. Organizational capacity to deliver training in biotechnology was strengthened via equipment purchases. DISSEMINATION ACTIVITIES: The MGCT workshop and activities of the grant were featured in the September, 2006 issue of the WVSU Research News Report. Activities of grant are also available at the website for the grant, http://fozzy.wvstateu.edu/mgct/. FUTURE INITIATIVES: In the coming semester, the Advanced DNA workshop, to be held on the WVSU campus in the Summer of 2007, will be planned. Scientists and students at other 1890 universities will be contacted and encouraged to submit proposals to MGCT faculty. It is hoped that several research projects can be augmented with a genomics component in this fashion. Additional students will travel to Cornell University for advanced biotechnical training as part of the grant.

Impacts
The project will positively impact students ranging from middle school to graduate school, either by exposing them to aspects of biotechnology and genomics, or increasing their capacity to perform research in those areas. WVSU's ability to provide genetic analysis services has been positively affected by equipment funded during this project.

Publications

  • No publications reported this period