Source: NORTH CAROLINA STATE UNIV submitted to NRP
GENETIC RELATEDNESS AND BEHAVIOR OF A SOLITARY MAMMAL: FORAGING ASSOCIATIONS AND KIN SELECTION IN BLACK BEARS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0204413
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2005
Project End Date
Sep 30, 2010
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
NORTH CAROLINA STATE UNIV
(N/A)
RALEIGH,NC 27695
Performing Department
Biology
Non Technical Summary
To understand behavior of animals, we need data on what the animals do as well as data on the genetic relatedness of animals. This project will determine whether female black bears that forage together are always relatives.
Animal Health Component
20%
Research Effort Categories
Basic
80%
Applied
20%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
13508301070100%
Knowledge Area
135 - Aquatic and Terrestrial Wildlife;

Subject Of Investigation
0830 - Wild animals;

Field Of Science
1070 - Ecology;
Goals / Objectives
I propose a long-term study of the spatial organization and genetic structure of black bears. By analyzing radiotelemetry, home range and food productivity data, and tissue samples (for DNA analysis) collected since 1988, in combination with additional, more detailed data collected using satellite telemetry over the coming 5 years, I shall have a substantial long-term data set with which to test hypotheses of behavioral-ecological theory and conservation relevance.
Project Methods
The 220 km2 Pisgah Black Bear Sanctuary lies in the Pisgah National Forest in the mountains of western North Carolina. My research team will live-trap black bears. All bears will receive unique eartags and tattoos. We shall draw blood into EDTA-treated vacutainers and clip a small section of skin from an ear of each bear for genetic analyses. The blood and ear tissue will be placed on ice immediately and frozen at -20 C upon leaving the field. All bears shall receive state-of-the art transmitter collars. Five adult female bears will receive GPS telemetry collars. We shall estimate bears' locations daily. I shall estimate bears' home ranges (utility distributions) using a fixed kernel estimator with band width chosen using least squares cross validation. Home range overlap will be quantified with Lloyd's (1967) overlap index. To test for a temporal component of overlap, I shall determine if females with overlapping home ranges are associated. I shall follow collared, breeding females to their dens in winter to sample their cubs. Cubs will be marked with unique ear notches for later identification (they are too small to be given eartags or tattoos) using clean, sharp scissors. To test my hypotheses about use of space by females and foraging associations, my research team will collect data on the productivity of major bear foods, during different seasons, throughout the study area. To do this, we shall expand upon our previous habitat sampling research. My research team extract DNA from the approximately 350 tissue samples (275 samples in hand, since 1988, and approximately 70 samples to be collected 2005-2009) using commercially available kits and amplify DNA using fluorescent dye-labeled primers for 8 microsatellite loci that are highly polymorphic in black bears, using previously established PCR protocols. Amplified products will be electrophoresed using an automated DNA sequencer (ABI 377) from which data will be collected using GENESCAN software. We shall use computer programs BIOSYS-1 and GENEPOP to calculate population genetic parameters, including allelic diversity, tests for Hardy-Weinberg Equilibrium, heterozygote deficiency, the presence of null alleles and linkage disequilibrium. We shall calculate individual relatedness from the microsatellite data using Queller & Goodnight's r, which measures the average relatedness of individuals within a group relative to the whole population and we shall use the computer program RELATEDNESS 5.0 to estimate pair-wise relatedness values for all female, male, and male-female pairs. Estimates of relatedness (r) will be calibrated for first order relatives, based on known mother-offspring pairs. To provide baseline information, we shall evaluate the mean relatedness of males and females with a two-sample randomization test, using the program RT 2.0. We shall compare mean relatedness estimates among males, females, and male-female pairs, to test for sex differences in relatedness.

Progress 10/01/05 to 09/30/10

Outputs
OUTPUTS: Long-term research begun in 1981 on the ecology and behavior of the black bear (Ursus americanus) population in the Pisgah Bear Sanctuary in the mountains of western North Carolina continued through 2009 with analyses of data collected through 2002 and publication of papers. We analyzed bears vital rates and population growth of black bears and showed that bears under different hunting regimes differ in the age-specific vital rates that are most important. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Long-term research begun in 1981 on the ecology and behavior of the black bear (Ursus americanus) population in the Pisgah Bear Sanctuary in the mountains of western North Carolina continued through 2009 with analyses of data collected through 2002 and publication of papers. We analyzed bears vital rates and population growth of black bears and showed that bears under different hunting regimes differ in the age-specific vital rates that are most important.

Publications

  • Mitchell, M S, L B Pacifici, J B Grand, & R A Powell. 2009. Contributions of vital rates to growth of a protected population of American black bears. Ursus 20: 77-84.


Progress 10/01/07 to 09/30/08

Outputs
OUTPUTS: Long-term research begun in 1981 on the ecology and behavior of the black bear (Ursus americanus) population in the Pisgah Bear Sanctuary in the mountains of western North Carolina continued through 2008 with analyses of data collected through 2002 and publication of papers. We analyzed bears' movements following capture, and blood samples and physical measurements collected during handling. We modelled home ranges of animals on a patchy landscape, similar to the landscapes occupied by bears. Because home ranges estimated from animal locations are largely continuous in space, the resource-bearing patches selected by an animal from a fragmented distribution of patches could be difficult to discern. PARTICIPANTS: Principal Investigator: Roger A Powell TARGET AUDIENCES: The scientific community, wildlife managers, environmental organizations PROJECT MODIFICATIONS: Funding has not become available for genetic analyses. Therefore, all aspects of the project dependent on genetic analyses have been put been suspended until funding becomes available.

Impacts
Analyses of bears' movements following capture show that activity is depressed for about 2 weeks before returning to normal levels. Struggling in snares can lead to muscle injury and frequent capture can retard growth by young bears. We modelled home ranges of animals on a patchy landscape, similar to the landscapes occupied by bears. The potential bias of including unselected, largely irrelevant patches in the field estimates of home ranges of animals can be high, particularly for home range estimators that assume uniform use of space within home range boundaries. Thus, inferences about the habitat relationships that ultimately define an animal's home range can be misleading where animals occupy landscapes with patchily distributed resources.

Publications

  • Cattet, M, J Boulanger, G Stenhouse, R A Powell & M J Reynolds-Hogland. 2008. An evaluation of long-term capture effects in ursids: implications for wildlife welfare and research. Journal of Mammalogy 89: 973-990.
  • Mitchell, M S & R A Powell. 2008. Estimated home ranges can misrepresent habitat relationships on patchy landscapes. Ecological Modelling 216: 409-414.


Progress 10/01/06 to 09/30/07

Outputs
Long-term research begun in 1981 on the ecology and behavior of the black bear (Ursus americanus) population in the Pisgah Bear Sanctuary in the mountains of western North Carolina continued through 2007. Analyses of vegetation data show that berry plants that provide important foods for bears are most abundant and produce the most berries during the 10 years following logging, then drop to very low levels for >60 years, and then rise to intermediate levels thereafter. Bears selected den sites far from gravel roads, on steep slopes, and at high elevations relative to what was available. Selection of den sites did not differ by sex or age, but did differ for bears of different reproductive status. Adult females with cubs preferred to den in areas that were relatively far from gravel roads while adult females without cubs did not.

Impacts
Because black bears are the only bears with high population sizes, broad distributions, and are not endangered, the information gained from this research can be applied to bear populations requiring conservation management. In addition, because all mammals have the potential for inbreeding and kin recognition, the understanding gained about home ranges can be applied to understand other mammals.

Publications

  • Reynolds-Hogland, M.J., Mitchell, M.S., and Powell, R.A. 2006. Spatio-temporal Availability of Soft Mast in Clearcuts in the Southern Appalachians. Forest Ecology and Management 237: 103-114.
  • Mitchell, M.S., and Powell, R.A. 2007. The optimal use of resources structures home ranges and spatial distribution of black bears. Animal Behaviour 74: 219-230.
  • Reynolds-Hogland, M.J., Mitchell, M.S., Brown, D. and Powell, R.A. 2007. Den site selection by black bears in the Southern Appalachians. Journal of Mammalogy 88:1062-1073.


Progress 10/01/05 to 09/30/06

Outputs
Long-term research begun in 1981 on the ecology and behavior of the black bear (Ursus americanus) population in the Pisgah Bear Sanctuary in the mountains of western North Carolina continued through 2006. Analyses of data show that the foraging behavior of bears during their active season is optimal and that foraging rules used by bears are the same as those used by small passerine birds foraging during each day in winter to be able to survive a night-time fast. Although trapping methods used in this research meet accepted standards for animal welfare, being trapping still affects foraging behavior, and probably social behavior, of black bears for at least 2 weeks following capture.

Impacts
Because black bears are the only bears with high population sizes, broad distributions, and are not endangered, the information gained from this research can be applied to bear populations requiring conservation management. In addition, because all mammals have the potential for inbreeding and kin recognition, the understanding gained about home ranges can be applied to understand other mammals.

Publications

  • Reynolds-Hogland, M. J., M. S. Mitchell, and R. A. Powell. 2007. Spatio-temporal Availability of Soft Mast in Clearcuts in the Southern Appalachians. Forest Ecology and Management
  • Mitchell, M. S., and R. A. Powell. 2007. The optimal use of resources structures home ranges and spatial distribution of black bears. Animal Behaviour.