Source: UNIV OF IDAHO submitted to NRP
MANAGEMENT AND ENVIRONMENTAL FACTORS AFFECTING MASTITIS CONTROL ON IDAHO DAIRIES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0204083
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2005
Project End Date
Jun 30, 2011
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF IDAHO
875 PERIMETER DRIVE
MOSCOW,ID 83844-9803
Performing Department
Animal & Veterinary Science
Non Technical Summary
Clinical and subclinical mastitis cause economic losses to milk producers and the dairy industry. This project examines the effect of cow cleanliness and the effect of facility design on milk quality.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
30734501060100%
Knowledge Area
307 - Animal Management Systems;

Subject Of Investigation
3450 - Milk;

Field Of Science
1060 - Biology (whole systems);
Goals / Objectives
The first objective of this study is to determine the effect of cow cleanliness during the close-up period on milk quality following calving in open-lot dairies. The second objective is to determine the effect of cow cleanliness during the first 7 days following dry-off on milk quality following calving in open-lot dairies. The third objective is to determine the effect of dairy facility design (free-stalls vs. open-lots) on milk quality and profile of mastitis pathogens in bulk tank milk samples.
Project Methods
Seven open-lot commercial herds located in Southern Idaho will be used in the trials. A minimum number of 525 close-up cows will be scored for cleanliness of udder and lower rear legs at least twice during the close-up period (525/7 = 75 cows per dairy). Udder and lower rear leg scores will be averaged and a composite score will be created. SCC data from the first post-calving test will be obtained from DHI and converted to a linear score. Clinical mastitis data will be collected from dairy records. Hygiene scores and clinical mastitis incidence will be correlated to SCC. The trial will be conducted in summer, winter, and spring during 2 consecutive years to account for seasonal and yearly variation. The same design will be repeated with dry cows in the first 7-10 days following dry-off. A minimum of 20 open lot dairies and 20 free-stall dairies will be visited in spring, summer, and winter and a bulk tank sample will be obtained from 5 different days, composited, and then analysed. The prevalence of contagious pathogens, environmental pathogens and minor pathogens will be determined and compared between both housing designs (free-stalls vs open lots). The average SCC during an entire year will be obtained and used as a variable in the correlation analyses. The correlation analyses will include the effect of bedding (sand vs. composted manure vs. shavings vs. straw).

Progress 07/01/05 to 06/30/11

Outputs
OUTPUTS: Several reserach studies were conducted to demonstarte the effect of cow cleanliness and facility design on milk quality and somatic cell counts in feedlot and openlot dairies in Idaho. The results of the research were incorporated in multiple milker trianing workshops. More than 1500 individuals attended the workshops which were presented in both English and Spanish. A curricula addressing milk quality as a well a serie of train the trainer slides were developped. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Dairy owners as well as English speaking and Spanish speaking employees were the target audience of this project. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
A pre- and post- test administered to a sample of 43 students attending the milker training found that the average score significantly improved from 55.3 to 82.6 percent. A follow-up with two dairies demonstrated that the milkers' increased knowledge of proper milking procedures decreased the incidence of subclinical and clinical mastitis and greatly impacted dairies profit.

Publications

  • Chahine, M., J. K. Reneau, R. J. Norell, J. C. Dalton. and J. M. Lukas. 2005. Relationship of cow cleanliness during the close-up period and milk quality following calving. J. Dairy Sci. 88(Suppl. 1):304.
  • Chahine, M. 2006. Spanish language educational opportunities for Idaho dairy employees-milker school. J. Dairy Sci. 89(Suppl. 1):185.
  • Chahine, M. 2007. Controlling mastitis through an appropriate milking routine. Controlar la mastitis a traves de unos procediminetos de ordeno adecuados. El lechero. September issue.
  • Chahine, M. and M. E. de Haro Marti. 2010. Como reducir el recuento de celulas somaticas (How to reduce Somatic Cell Counts). Albeitar, Spain. July 2010 (136): 4-6.
  • de Haro, M. E and M. Chahine, M. 2011. Como reducir el recuento de celulas somaticas (How to reduce somatic cell counts). http://www.pymeslacteas.com.ar/articulos.phpid=381, February 2011.
  • Chahine, M. 2011. Teat dipping and the cold weather. Dairy Focus Newsletter. February 2011.
  • de Haro, M. E and M. Chahine. 2011. Como reducir el recuento de celulas somaticas (How to reduce somatic cell counts). http://www.ganaderia.com.mx/ganaderia/home/articulos.aspcve_autor=80 1 January 2011.


Progress 01/01/10 to 12/31/10

Outputs
OUTPUTS: -More than 1500 dairy workers have completed milk quality training in both English and Spanish -Results of the research project have been disseminated through numerous milker schools that have been conducted by the PI across the state of Idaho -Materials associated with improving milk quality on dairies have been posted in both English and Spanish on the daireXnet web site. PARTICIPANTS: Principal Investiagtor: Dr. Mireille Chahine Collaborators: eXtension, Extension dairy specialists at other land grant universities, Idaho dairy producers, extension educators, allied industry members. TARGET AUDIENCES: Dairy owners, dairy employees, extension educators. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
-Significant decrease in SCC and improvement in milk quality have been achieved on collaborating dairies. -Recommendations have been extended summarizing the effect of maintining a clean environment on freestall and open lot dairies on cleanliness scores and milk quality.

Publications

  • No publications reported this period


Progress 01/01/09 to 12/31/09

Outputs
OUTPUTS: Data collection and laboratory analyses of bulk tank samples from Idaho dairies have been completed. Data are currently being summarized to determine pertinent conclusions. Preliminary results from the studies have been included in several clientele presentations and have been disseminated through milker schools to English and Spanish speaking dairy employees. PARTICIPANTS: Several dairy producers, University of Idaho graduate students and dairy workers. TARGET AUDIENCES: Dairy producers and dairy employees (English and Spanish speaking). PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Preliminary results from the project demonstrate a greater effect of individual dairy management practices on milk quality on Idaho dairies exceed the effect of facility type (freestall or open-lot).

Publications

  • No publications reported this period


Progress 01/01/08 to 12/31/08

Outputs
OUTPUTS: Funds were used to purchase a CO2 incubator which is needed to determine the presence of mycoplasma in bulk tank samples that were collected last year. We are in the process of analyzing bulk tank samples that were collected last year from forty dairies in Twin Falls, Jerome and Gooding Counties. We will also be starting a new study examining the effect of cleanliness of close-up cows immediately prior to calving on SCC and infection status in early lactation. Data were also obtained from DHIA and are currently being analyzed to determine the effect of season on SCC on Idaho dairies. PARTICIPANTS: Two undergraduate students are currently helping with the culturing process in the lab. A research scholar from Bolivia has worked on setting up the milk culturing protocol. TARGET AUDIENCES: Target audiences are Idaho dairy producers as well as Idaho dairy employees. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Preliminary data from the study were shared with participants in milker schools that were conducted in Southern Idaho. This study is allowing us to generate milk quality management recommendations that are applicable to Idaho's high desert conditions.

Publications

  • No publications reported this period


Progress 01/01/07 to 12/31/07

Outputs
OUTPUTS: Fourty dairies (20 openlots and 20 freestalls) in Twin Falls, Jerome, and Gooding counties were visited and a bulk tank sample were obtained from several days and composited. Milk samples were frozen and will be analysed in summer 2008 for Streptococcus agalactiae, Staphylococcus aureus, Streptococcus non-agalactiae, coliforms and coagulase-negative. The prevalence of contagious pathogens, environmental pathogens and minor pathogens will be determined and compared between both housing designs (freestalls vs open lots). The average SCC during an entire year will be obtained and used as a variable in the correlation analyses. PARTICIPANTS: Dr. Mireille Chahine (PI)

Impacts
Resources provided allowed us to collect milk bulk tank samples from different dairy housing types. Those samples will be analysed for contagious and environmental mastitis. Resources provided will also allow a graduate student to perform the analysis in 2008.

Publications

  • No publications reported this period


Progress 01/01/06 to 12/31/06

Outputs
During the last year, we have been extensively preparing the lab for culturing capacity. This is necessary in order to be able to determine the effect of dairy facility design (free-stalls vs. open-lots) on milk quality and profile of mastitis pathogens in bulk tank milk samples. When we are ready to run the samples, we will be collecting samples from a minimum of 20 open-lot dairies and 20 free-stall dairies. Visits will occur in spring and summer 2007 and in winter 2008, and a bulk tank sample will be obtained from 5 different days, composited, and then analysed. Milk samples will be frozen and subsequently cultured to determine the presence of contagious (Streptococcus agalactiae, Staphylococcus aureus, and coagulase-negative) and environmental (Streptococcus non-agalactiae and coliforms) pathogens. The prevalence of contagious pathogens, environmental pathogens and minor pathogens will be compared between both housing designs (free-stalls vs open lots). The average SCC during an entire year will be obtained and used as a variable in the correlation analyses. The correlation analyses will include the effect of bedding (sand vs. composted manure vs. shavings vs. straw).

Impacts
Results are not yet available for the past year. It is anticipated, that when completed, this part of the project will allow us to determine the effect of dairy facility design (free-stalls vs. open-lots) on milk quality and profile of mastitis pathogens in bulk tank milk samples during different seasons. This will provide dairy producers with more information to decide what type facility they prefer to build or purchase.

Publications

  • No publications reported this period


Progress 07/01/05 to 12/31/05

Outputs
Seven open-lot commercial herds located in Southern Idaho were used in the study. A scoring system from 1 to 5 was selected. Score 1 indicates a cow that is absolutely clean while a score 5 indicates a very dirty cow. A total of 681 close-up cows were hygiene scored at least twice during the close-up period. Each cow was scored for cleanliness of udder (HU) and lower rear legs (HL). Udder and lower rear leg scores were averaged and a composite score was created (HUL). SCC data were obtained from DHI and converted to a linear score (LS). HU, HL, and HUL were correlated to SCC and LS using PROC CORR and regression analyses were conducted using PROC REG of SAS. SCC for the 681 cows on their first DHI test date following calving averaged 251 x 103 cells/ml. HU, HL, and HUL averaged 2.3, 3.1, and 2.7 respectively. HU were significantly correlated to HL (P < 0.001, R2 = 0.57). There were no significant correlations between SCC and the hygiene scores (P > 0.05). LS was significantly correlated to HU and HUL (P < 0.03) but not correlated with HL (P > 0.05). However, using a linear regression model in which HU and HUL were the explanatory variables and LS was the dependent variable resulted in a very low R2 (R2 < 0.10).

Impacts
Dependence of first SCC and LS following calving on hygiene scores during the close-up period in the high desert area of Southern Idaho is either absent or relatively small in cattle housed in open-lot facilities during the dry summer season.The study will be repeated in Spring and is anticipated to demonstrate to dairy producers the importance of accurately managing their facilities in the short muddy period that occur in Spring ultimaltely leading to a decrease in subclinical mastitis.

Publications

  • Chahine, M., J. K. Reneau, R. J. Norell, J. C. Dalton. And J. M. Lukas. 2005. Relationship of cow cleanliness during the close-up period and milk quality following calving. J. Dairy Sci. 88(Suppl. 1):304.