Progress 09/01/05 to 08/31/10
Outputs
The objectives were the successful training, graduation and placement of highly trained individuals into fields to benefit agriculture. As detailed below, we have been able to train and graduate one of the students, and the other student, who began his graduate work only three years ago, is completing draft publications of his research and is expected to graduate in 2011. Sarah Beth Redmond presented her research at the 2010 Plant and Animal Genome Conference in San Diego, CA; the Genetics of Animal Health Symposium in Paris, France; and the Poultry Science Association Annual Meeting in Denver, CO. Ms. Redmond is first author of an article published in Poultry Science, and first author or co-author on 6 manuscripts that have been submitted for publication. Research from Ms. Redmond's dissertation will be presented in the Poultry Workshop at the 2011 Plant and Animal Genome Conference. Ms. Redmond successfully defended her dissertation and completed training through the Preparing Future Faculty and Graduate Student Teaching Certification programs. Ms. Redmond also received several awards for excellence in graduate teaching and research. Ms. Redmond has begun post-doctoral training in the Biology Department of DePauw University under Dr. Janet Vaglia. Curt Christian attended two national conferences and presented initial results on pathway analysis of porcine response to Salmonella infection in whole blood at the International Plant and Animal Genome conference in January 2009. Mr. Christian completed work on a project investigating the porcine macrophage response to Salmonella endotoxin using state-of-the-art transcriptional profiling methods, while additionally investigating the NFkB pathway in this response by use of the NFkB pathway inhibitory drug SC-514. Mr. Christian is currently writing a first-author manuscript describing this work that will be submitted early in 2011. He is also a co-author on two other manuscripts to be submitted for publication in 2011 by a Ph.D. student collaborator as well as a postdoctoral collaborator. PRODUCTS: Altogether the two students supported on this project have attended and presented their research at 10 different conferences, and nine abstracts have been published. Sarah Beth Redmond presented components of her research at three conferences, published six abstracts, and prepared and submitted three first author manuscripts, one of which has been published in Poultry Science. Curt Christian presented initial pathway analysis results as a poster at the Plant and Animal Genome Conference in San Diego, California in January of 2009, and two other presentations have been made at conferences that included his work; all of these have been published as abstracts. Mr. Christian has co-authored two manuscripts that will both be submitted for publication early in 2011. OUTCOMES: Sarah Beth Redmond is an accomplished PhD. scientist who has been placed into a postdoctoral research lab. She has completed training to prepare her to become a future faculty member, and has had the opportunity to gain experience in teaching at Iowa State University both as a teaching assistant and as a sole instructor of AnS 233, Poultry Science. Curt Christian is trained in molecular biology and bioinformatics approaches and is currently completing his thesis research and has gained experience in manuscript development and completion. DISSEMINATION ACTIVITIES: Eight manuscripts and abstracts have been published, and 12 posters have been presented by the Fellows or by collaborators presenting work performed in part by the Fellows. FUTURE INITIATIVES: Based on the published and unpublished research of these students, we plan to prepare grant proposals to further the projects to which they contributed. Also, due to the success of this training model, we submitted a new National Needs proposal to train students in the area of immunogenomics and immunogenetics as this is an important National Need area due to the increasing emphasis on Animal and Food Safety.
Impacts
In addition to the research results that these students have already produced and will create in the future, the creation of well-trained science professionals will positively impact the agricultural and biological knowledge base and human resources base for agricultural research for many years to come.
Publications
- Christian, C., O. Couture, C. Y. Demirkale, D. Nettleton, J.J. Uthe, S.M.D. Bearson, V. Honavar, C. K. Tuggle. Identification of Common Functions of Differentially Expressed Gene Sets in Porcine Whole Blood in Response to Infection with Salmonella enterica serovar Typhimurium Plant and Animal Genome meeting: January 10-14, 2009, San Diego; abstract #P641; page 120.
- Redmond, S.B., R. Tell, D. Coble, C. Mueller, C.B. Andreasen, D. Palić, and S.J. Lamont. 2010. Differential responses to dietary immune modulation by diverse chicken lines measured via expression of cytokine and chemokine mRNA in the spleen. Poultry Science. 89:1635-1641.
- Redmond, S.B., P. Chuammitri, D. Palić, C.B. Andreasen, and S. J. Lamont. 2010. Genetic line and dietary immunomodulator effects on expression of CXCLi2 in chicken heteorphils responding to Salmonella enteritidis. In: Poult. Sci. 89:E-Suppl. 1, abstract 271.
- Redmond, S.B., P. Chuammiti, C.B. Andreasen, D. Palić, and S.J. Lamont. 2010. SNP association of chicken heteorphil funcitonal traits and response to dietary β-glucans in two advanced intercross lines [abstract #12]. In: Proceedings of the 2nd Animal Genomics for Animal Health Conference. May 21-June 2. Maison al la Chiame, Paris, France.
- Redmond, S.B. and S.J. Lamont. 2010. SNP association analysis of two chicken advanced intercross lines for growth [abstract #588]. In: Proceedings of Plant and Animal Genomes XVIII Conference. January 9-13. Town and Country Convention Center, San Diego, California.
- Coble, D.J., S.B. Redmond, B. Hale, and S.J. Lamont. 2010. The effect of Salmonella enteritidis on immune genes in genetically distinct lines of chickens [abstract #74]. In: Proceedings of the 15th Pacific Symposium on Biocomputing. January 4-8. Fairmont Orchid, Big Island of Hawaii.
- Tuggle, C.K., Huang, T., Couture, O., Ramer-Tait, A., Christian, C. Uthe, U., Demirkale, C. Y., Ji, T., Nettleton, D., Wannemuehler, M.J., and S.M.D. Bearson. The porcine transcriptomic response to Salmonella Typhimurium and its endotoxin. The International. Plant & Animal Genome XIX Conference. January 15-19, 2011, San Diego, California.
- Tuggle, C.K. Jolita J Uthe, C. Christian, O. Couture, C.Y. Demirkale, D. Nettleton, V. Honavar, J.K. Lunney, S.M.D. Bearson. 2010. Developing Predictive Models For Identifying Pigs With Superior Immune Response And Improved Food Safety. The International. Plant & Animal Genome XVIII Conference. January 9-13, 2010, San Diego, California. Abstract #W610, page 156.
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Progress 09/01/08 to 08/31/09
Outputs
The objectives are the successful training, graduation and placement of highly trained individuals into the above fields to benefit agriculture. Both Fellows (Sarah Beth Redmond, Curtis Christian) are being trained for the PhD. Sarah Beth Redmond presented some of her work at the July 2009 Systems Biology themed Growth Factors and Signal Transduction Symposium in Ames, IA, and a manuscript is in preparation. Additional research by Ms. Redmond will be presented at the 2010 Plant and Animal Genome Conference in San Diego, CA. The initial experiments from Ms. Redmond's work have been published in Veterinary Immunology and Immunopathology. Ms. Redmond was awarded a scholarship to attend the Wakonse Conference on College Teaching in May 2009, and completed training through the Preparing Future Faculty and Graduate Student Teaching Certification programs. Curt Christian presented initial results on pathway analysis of porcine response to Salmonella infection in whole blood at the Plant and Animal Genome conference in January 2009. Currently he is optimizing a chromatin immunoprecipitation procedure to study transcriptional networks in vivo. The initial work looking at activation of the NF-ĸB pathway in RAW 264.7 murine macrophage cell line should be completed soon and prepared as part of a manuscript to submit for publication as a co-author. PRODUCTS: Several manuscripts have been published and several posters have been presented in the last year by the fellows or by collaborators presenting work performed in part by the Fellows. OUTCOMES: Sarah Beth Redmond completed the experiments that will be included in her dissertation and is first-author or co-author on four manuscripts currently in preparation. Curt Christian presented initial pathway analysis results as a poster at the Plant and Animal Genome Conference in San Diego, California in January of 2009. DISSEMINATION ACTIVITIES: Several manuscripts have been published and several posters have been presented in the last year by the fellows or by collaborators presenting work performed in part by the Fellows. FUTURE INITIATIVES: It is anticipated that the two Fellows will complete their studies and obtain the degree they are seeking.
Impacts
In addition to the research results that these students are creating currently as well as in the future, the creation of well-trained science professionals will positively impact the agricultural and biological knowledge base and human resources base for agricultural research.
Publications
- Redmond, SB, P Chuammitri, CB Andreasen, D Palic, and SJ Lamont. 2009. Chicken heterophils from commercially selected and non-selected genetic lines express cytokines differently after in vitro exposure to Salmonella enteritidis. Vet. Immunol. Immunopathol. 132:129-134.
- Chuammitri, P, J Ostojic, CB Andreasen, SB Redmond, SJ Lamont, and D Palic. 2009. Chicken heterophil extracellular traps (HETs): novel defense mechanism of chicken heterophils. Vet. Immunol. Immunopathol. 129:126-131.
- Redmond, SB, R Tell, D Coble, C Mueller, D Palic, CB Andreasen, and SJ Lamont. Immuno nutrigenomics of the chicken spleen [abstract]. In: Proceedings of the 18th Annual Growth Factors and Signal transduction Symposium. 2009 June 11-14.
- Christian, C., O. Couture, C. Y. Demirkale, D. Nettleton, J.J. Uthe, S.M.D. Bearson, V. Honavar, C. K. Tuggle. Identification of Common Functions of Differentially Expressed Gene Sets in Porcine Whole Blood in Response to Infection with Salmonella enterica serovar Typhimurium Plant and Animal Genome meeting: January 10-14, 2009, San Diego; abstract #P641; page 120.
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Progress 09/01/07 to 08/31/08
Outputs
Sarah Beth Redmond (maiden name Sherrill) presented initial research project results as a poster at the Avian Immunology Research Group meeting in Gold Coast, Australia in June 2008. Ms. Redmond is a coauthor on a manuscript from a collaborating lab that is currently under review. Ms. Redmond also mentored an undergraduate intern in a project based on her own work. Curt Christian completed 3 rotations as required by the interdepartmental genetics program. The first was with Dr. Matthew Ellinwood working with the lysosomal storage disease Mucopolysacharidosis. The second was with Dr. Chris Tuggle doing Electromobility Shift Assays to determine binding motifs of the transcription factor NFκB. The final rotation was with Dr. Vasant Honavar working on developing an algorithm to predict secondary protein structure. PRODUCTS: S. Redmond has begun preparing a manuscript for publication, and presented initial research results as a poster at the Avian Immunology Research Group meeting in Australia in June 2008. The initial results from C. Christian's research will be presented as a poster at the Plant and Animal Genome Conference in San Diego, California in January of 2009. In addition to publications listed, the products of this Training grant will be trained doctoral students who can contribute to state-of-the-art agricultural research in the future.in the future OUTCOMES: Ms. Redmond completed the first planned portion of her research project. She is a coauthor on a manuscript from a collaborating lab that is currently under review. C. Christian decided to join the lab of Dr. Tuggle for his thesis work and has begun formulating a research project. The initial results from this will be presented as a poster at the Plant and Animal Genome Conference in San Diego, California in January of 2009. At the end of the grant period, two students will be trained as animal geneticists. DISSEMINATION ACTIVITIES: Sarah Beth Redmond presented her research results as a poster at the Avian Immunology Research Group meeting in Gold Coast, Australia in June 2008. Curt Christian will be presenting his preliminary studies at the Plant and Animal Genome meetings in January 2009 in San Diego. FUTURE INITIATIVES: None at this time
Impacts
In addition to the research results that these students are creating currently as well as in the future, the creation of well-trained science professionals will positively impact the agricultural and biological knowledge base and human resources base for agricultural research.
Publications
- Redmond, Sarah Beth, Phongsakorn Chuammitri, Dusan Palic, Claire Andreasen, and Susan J. Lamont. Genetic differences in expression of immune response genes by chicken heterophils stimulated with Salmonella enteritidis [abstract]. In: Proceedings of the Avian Immunology Research Group; 2008 June 24-27; Gold Coast, Australia. Abstract number 40.
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Progress 09/01/06 to 08/31/07
Outputs
In this reporting period, we have completed the second recruiting season. We interviewed over 7 U.S. applicants from a pool of 40 such applicants. We made offers and successfully enrolled one Fellow into the program, to finish our recruiting for two National Needs Fellowship positions. This Fellow matriculated to Ames in summer 2007 and initiated exploratory rotation experiences in August 2007.
PRODUCTS: We have recruited a second Fellow to our training group, to complete our Fellows roster. This person is Curt Christian.
OUTCOMES: Our first Fellow, S.B. Sherrill, participated successfully in two additional research rotations in October 2006-April 2007. She joined the lab of S.J. Lamont in Spring 2007. Research completed by Ms. Sherrill during Summer 2007 will contribute to a manuscript in preparation by members of the labs of Claire Andreasen and Dusan Palic. Our second Fellow, C. Christian, participated successfully in his first research rotation in August 2007.
DISSEMINATION ACTIVITIES: We disseminated specific USDA National Needs recruiting materials to Midwest colleges and universities, as well as posted similar materials at many meetings during 2007. We also send announcements of available Fellowships to more than 1,200 scientists working in the animal genomics fields via electronic mail.
FUTURE INITIATIVES: Mr. Christian will complete his exploratory laboratory rotations during 2007-8 and select his permanent thesis advisor in the Spring of 2008.
Impacts
Our proposed and now initiated training of PhD students in Animal Molecular Biology, Genomics and Bioinformatics will provide excellent and broadly educated professional that will be able to address new opportunities in utilizing genome tools and information to improve animal genetics for US agriculture.
Publications
- No publications reported this period
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Progress 09/01/05 to 09/01/06
Outputs
In this reporting period, we have completed the first recruiting season. We interviewed over 10 U.S. applicants from a pool of over 30 such applicants. We made offers to two outstanding students and successfully enrolled one Fellow into the program. This Fellow matriculated to Ames in summer 2006 and initiated exploratory laboratory rotation experiences in August 2006.
PRODUCTS: We have recruited one Fellow to our training group (S.B. Sherrill).
OUTCOMES: Our first Fellow, S.B. Sherrill, participated successfully in her first research rotation in August 2006. Research initiated by Ms. Sherrill will be part of a manuscript in preparation by members of the rotation lab of Lisa Nolan.
DISSEMINATION ACTIVITIES: We disseminated specific USDA National Needs recruiting materials to Midwest colleges and universities, as well as posted similar materials at many meetings during 2006. We also send announcements of available Fellowships to more than 1,200 scientists working in the animal genomics fields via electronic mail.
FUTURE INITIATIVES: We will recruit a student to fill our last Fellowship position during the 2006-2007 recruiting season. Ms. Sherrill will complete here exploratory laboratory rotations duign 2007 and select her permanent thesis advisor in the Spring of 2007.
Impacts
Our proposed and now initiated training of PhD students in Animal Molecular Biology, Genomics and Bioinformatics will provide excellent and broadly educated professional that will be able to address new opportunities in utilizing genome tools and information to improve animal genetics for US agriculture.
Publications
- No publications reported this period
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