Progress 06/01/05 to 07/31/06
Outputs Brimrose developed a handheld miniature spectrometer capable of measuring sucrose content in cut pieces and whole sugar beets. The spectrometer is based on acousto-optic tunable filter (AOTF) technology. An AOTF device consists of a thin plate of a piezoelectric transducer mounted on a piece of AO crystal. By applying electric radio frequency (RF) signals to the electrodes on the transducer faces, acoustic waves are generated by way of the piezoelectric effect and then transmitted into the AO crystal. Through AO diffraction, optical beams passing through the AO crystal can thus be manipulated (deflected, modulated, or diffracted) by electric signals applied to the transducer. Acousto-optic tunable filter (AOTF) spectrometers have several advantages over traditional spectrometers. Traditional spectrometers require careful handling and frequent calibration. They also suffer lower scan speed and lower reliability. An AOTF is an all solid-state tunable filter with no
moving parts and is therefore immune to orientation changes or even severe mechanical shock and vibrations, making it ideal for operation in harsh industrial environments. Moreover, the AOTF is a high throughput and high-speed programmable device capable of randomly accessing thousands of precise wavelengths in less than a second, making it an excellent tool for in-situ near infrared (NIR) spectroscopy. AOTF technology is a novel engineering approach to measure sucrose content in cut pieces, whole sugar beets, and/or sugar beet brei. This engineering approach will aid biological/genomic approaches to develop a strategy to enhance the efficiency of sugar beet crop production. This work will provide a better understanding of the dynamic of sucrose accumulation in roots during the growing season and may help to determine better selection targets in order to maximize total sucrose (e.g. recoverable white sugar per beet) at the end of the season. This information may also be used to
maximize the efficiency of selection early in the season among divergent breeding lines. These measurements are theoretically non-destructive, raising a potential to perform repeated measurements over the season of the same sugar beet plants. Project was to combine an engineering approach with a biological approach to maximize total sucrose in the sugar beet (e.g. recoverable white sugar per beet) at the end of the season. We developed a simple, compact, and cost-efficient piece of equipment based on an AOTF spectrometer to measure the sucrose content in cut pieces and whole sugar beets. Such measurements will provide a better understanding of the dynamic of sucrose accumulation in roots during the beginning of the growing season, and information may be used to maximize the efficiency of selection early in the season among divergent breeding lines. Using AOTF-based spectrometry to monitor the sucrose content of the sugar beet is non-destructive giving the possibility to perform
repeated measurements over the season of the same sugar beet plants. It is suitable for examining large populations of individuals as they exist in advanced breeding programs.
Impacts The goal of this work was to develop simple, compact, and cost-efficient equipment that sugar beet growers can afford and easily operate. Using AOTF technology is a novel engineering approach to measure sucrose content in cut pieces, whole sugar beets, and/or sugar beet brei. This engineering approach will aid biological/genomic approaches to develop a strategy to enhance the efficiency of sugar beet crop production. This work will provide a better understanding of the dynamic of sucrose accumulation in roots during the growing season and may help to determine better selection targets in order to maximize total sucrose (e.g. recoverable white sugar per beet) at the end of the season. This information may also be used to maximize the efficiency of selection early in the season among divergent breeding lines. These measurements are theoretically non-destructive, raising a potential to perform repeated measurements over the season of the same sugar beet plants.
Publications
- No publications reported this period
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