Progress 12/01/01 to 04/30/05
Outputs
For these studies a dry powder aerosol generator was designed that is capable of producing well dispersed dust suspensions using only a small quantity of PM2.5-enriched powders. This system was developed to allow for dust resuspension for inhalation experiments with limited amounts of coal flyash materials collected and size-fractionated from source sampling. Premixing of the test powder with large diameter glass beads (100 mm), the device is able to deliver concentrations from 100 - 1000 mg/m3 to a test chamber using only a few milligrams of the test powder per hour. Scanning electron microscope examination of filter samples show the aerosol contains well-dispersed particles resuspended from the test powder with no evidence of glass bead fragments. This unique system for coal flyash allows us to measure biological effects due to inhalation of well-characterized sample materials that are available in only limited quantities.
Impacts
This work shows short, repeated exposure to coal flyash can produce a small, but significant inflammatory response in the lungs of young adult rats. This inflammatory response due to particle exposure also shows evidence of systemic passage of the particles or inflammatory mediators into the circulation, suggesting further adverse health effects associated with exposure to the byproducts of coal combustion.
Publications
- Jasinski, J., Pinkerton, K.E., Kennedy, I.M., Leppert, V.J. 2005. Surface oxidation state of combustion-synthesized V-Fe2O3 nanoparticles determined by electron energy loss spectroscopy in the transmission electron microscope. Sensors and Actuators B 109:19-23.
- Teague, S.V., Veranth, J.M., Aust, A.E., Pinkerton, K.E. 2005. Dust Generator for Inhalation Studies with Limited Amounts of Archived Particulate Matter. Aerosol Science and Technology 39:85-91.
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Progress 01/01/04 to 12/31/04
Outputs
Premixing of the test particles with larger diameter glass beads permits delivery of aerosol concentrations from 100-1000 micrograms/m3 to an exposure chamber using only milligrams quantities of the test powder per hour. Examination of exposure chamber filter samples by scanning electron microscopy showed well-dispersed particles of the test powder free of glass spheres or fragments. Data are presented from experiments using coal fly ash as the test powder to illustrate the system performance.
Impacts
A novel design for a dry-aerosol generator that efficiently produces a well-dispersed dust suspension using small quantities of a PM2.5-enriced powder sample is described. The motivation to develop a highly efficient dry-aerosol particle generator was to facilitate collaborative projects that combine in vitro cell culture experiments and multi-day inhalation exposures using a single batch of well-characterized particles.
Publications
- No publications reported this period
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