Source: UNIV OF HAWAII submitted to NRP
CONTROL OF COQUI FROG POPULATIONS IN HAWAII
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0201517
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2003
Project End Date
Sep 30, 2006
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF HAWAII
3190 MAILE WAY
HONOLULU,HI 96822
Performing Department
MOLECULAR BIOSCIENCES & BIOSYSTEMS
Non Technical Summary
Coqui frogs are reproducing rapidly in Hawaii because they lack natural predators. Their levels are about ten times the levels in their native Puerto Rico. They disturb the sleep of residents and visitors and may put endangered bird species at risk by competing for food insects and serving to raise populations of rats and mongooses which are predators of both frogs and native birds. The purpose of the project is to control coqui frog populations through use of pesticides. Two groups of pesticides are currently being thought about, lime and caffeine/pyrethrin.
Animal Health Component
70%
Research Effort Categories
Basic
10%
Applied
70%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1350860107030%
1353899115070%
Goals / Objectives
1) Determine the efficacy of the 1% caffeine/0.01% pyrethrin cocktail in fields when sprayed at low rates (100 gal/acre). This rate contrasts to so called drench sprays. 2) Develop methods of counting coqui frogs in the field. 3) Determine needed re-treatment intervals for pesticides.
Project Methods
1) To determine the efficacy of the 1% caffeine/0.01% pyrethrin cocktail whether laboratory studies using pyrethrins and culled coffee bean extracts are efficacious in the field, conduct field trials after spraying a mist at 100 gal/acre. Such trials require permits from the Institutional Animal Care and Use Committee and the State Department of Agriculture (for pesticide application). 2) Preliminary studies suggest counted frogs in treated and control plots will not be easy. No one has published pesticide efficacy data probably for this reason. The areas in which they live are heavily overgrown. Counting frogs in control and treatment areas requires extremely manpower intensive and time consuming clearing of the brush. Moreover, clearing of the brush changes things so each counting may only be done once (not before and after spraying). On the other hand, counting frogs at night by listening for them and identifying them after illuminating them with flashlights risks under-counting as females do not call at night. Frogs that do not come out from under cover to call and breed may also be missed. Several counting experiments followed by statistical analysis will be conducted. 3) To determine the time intervals for re-treatments, repeat counts in treated areas at time intervals after a treatment/kill.

Progress 10/01/03 to 09/30/06

Outputs
Coqui frogs are a nuisance in Hawaii emitting loud shrieks keeping residents awake at night. They threaten the potted plant industry because they often hide away in plants and recipient importers threaten banning Hawaii plants. Coqui frogs appear to be depleting the ecosystem of insect prey and therefore could alter the ecology of Hawaii. Initial work was with caffeine and pyrethrin which were identified by others as potential pesticides. The toxicities of caffeine and pyrethrin were tested in the laboratory and in the field. Their effects were found to be additive and not synergistic. It was also observed that concentrated populations of coqui frogs in Hawaii that were reported in the gray literature could not be confirmed. Populations were relatively low. Effective concentrations of caffeine were at levels that would be toxic to mammals. For this reason it was concluded that caffeine and pyrethrin should not be pursued as remedies for the coqui frog. The project moved forward to warm water disinfestation which will be reported under another number.

Impacts
Work on caffeine and pyrethrin was terminated. Work was begun on warm water disinfestations.

Publications

  • No publications reported this period


Progress 10/01/04 to 09/30/05

Outputs
Coqui frogs are a nuisance in Hawaii emitting loud shrieks keeping residents awake at night. Also, they threaten the potted plant industry because they often hide away in plants and recipient importers threaten banning Hawaii plants. Initial work was with caffeine and pyrethrin. This proved not to be a desirable treatment. Current work is with hot water disinfectation of potted plants going out for sale. With the help of Charles Nelson, hot water units were constructed. They all have propane burner heaters and are designed to re-use hot water. The prototype was sent to Hilo for trials by Arnold Hara. A large unit was built for a farm on Oahu. This unit will be used by all the farmers who care to use it. Plans were prepared for the State of Hawaii Department of Agriculture. They will build units for stakeholders around the state.

Impacts
Threats to ban import of Hawaii potted plants will disappear because plants will be hot water treated.

Publications

  • No publications reported this period


Progress 10/01/03 to 09/30/04

Outputs
Simulated field trials in the laboratory were conducted using a 1% caffeine/0.01% pyrethrin cocktail on coqui frog. When hidden in moss such as in the day, the pesticide was ineffective even though it was lethal when frogs were in bare aquaria devoid of cover. Field trials were initiated. They were conducted at night when frogs emerge from cover to feed and breed. Counting was explored and statistically reproducible values were obtained before and after spraying were counted as a grid. The counting was very labor intensive and relied on prior training of the counter. The counting may not be as accurate as toe clipping methods discovered in the literature after the current studies were started but would probably draw less attention than methods relying on cutting off frog toes without anesthetic. Other methods of validation were tried but preliminary data suggest that spraying must be done at night and that an 80% kill rate may be expected.

Impacts
More effective methods for determining pesticide efficacy have been developed. They require less time and manpower than existing methods.

Publications

  • No publications reported this period